Name: Haloplanus salinarum SP28
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 158747

Type strain:

Species: Haloplanus salinarum

Strain Designation: SP28

Culture col. no.: JCM 31424, KCCM 43210

Strain history: S.-J. Park; Dept. of Biol., Jeju Natl. Univ., South Korea; SP28.

NCBI tax ID(s): 1912324 (species)

Section

Haloplanus salinarum SP28 is an obligate aerobe, Gram-negative, motile archaeon that forms circular colonies and was isolated from brine sample from a solar saltern.

colony-forming
Gram-negative
motile
pleomorphic-shaped
obligate aerobe
16S sequence
Archaea
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Archaea"
	Phylum	*Methanobacteriota*
	Class	*Halobacteria*
	Order	*Haloferacales*
	Family	*Haloferacaceae*
	Genus	*Haloplanus*
	Species	**Haloplanus salinarum**
	Full Scientific Name (LPSN)	Haloplanus salinarum Hwang et al. 2017

Information on morphological and physiological properties Morphology

[Ref.: #65498]	Gram stain	negative

[Ref.: #65498]	Cell length	0.6-3.5 µm

[Ref.: #65498]	Cell width	0.6-2.3 µm

[Ref.: #65498]	Cell shape	pleomorphic-shaped

[Ref.: #65498]	Motility	yes

[Ref.: #65498]	Colony color	red
[Ref.: #65498]	Colony shape	circular

Information on culture and growth conditions Culture and growth conditions

[Ref.: #65498]

Culture medium

DBCM2 agar

[Ref.: #65498]

Culture medium growth

[Ref.: #65498]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #65498]	growth	25-50 °C
[Ref.: #65498]	optimum	37 °C
[Ref.: #67770]	growth	37 °C

[Ref.: #65498]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #65498]			optimum	8.0
[Ref.: #65498]			growth	6.5-8.5

Information on physiology and metabolism Physiology and metabolism

[Ref.: #65498]

Oxygen tolerance

obligate aerobe

[Ref.: #65498]

Halophily / tolerance level

extremely halophilic

	Salt		Salt conc.
[Ref.: #65498]	NaCl	growth	2.5-4.5	M
[Ref.: #65498]	NaCl	optimum	3.1	M
[Ref.: #65498]	MgCl2	growth	0.05-0.5	M
[Ref.: #65498]	MgCl2	optimum	0.1	M

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #65498]	casein	-	hydrolysis
[Ref.: #65498]	D-fructose ChEBI	-	builds acid from
[Ref.: #65498]	D-fructose ChEBI	-	carbon source
[Ref.: #65498]	D-fructose ChEBI	-	energy source
[Ref.: #65498]	D-galactose ChEBI	-	builds acid from
[Ref.: #65498]	D-galactose ChEBI	-	carbon source
[Ref.: #65498]	D-galactose ChEBI	-	energy source
[Ref.: #65498]	D-glucose ChEBI	-	builds acid from
[Ref.: #65498]	D-glucose ChEBI	-	carbon source
[Ref.: #65498]	D-glucose ChEBI	-	energy source
[Ref.: #65498]	D-xylose ChEBI	+	carbon source
[Ref.: #65498]	D-xylose ChEBI	+	energy source
[Ref.: #65498]	dimethyl sulfoxide ChEBI	-	electron acceptor
[Ref.: #65498]	dl-alanine ChEBI	+	carbon source
[Ref.: #65498]	dl-alanine ChEBI	+	energy source
[Ref.: #65498]	dna ChEBI	-	hydrolysis
[Ref.: #65498]	gelatin ChEBI	-	hydrolysis
[Ref.: #65498]	glycine ChEBI	-	carbon source
[Ref.: #65498]	glycine ChEBI	-	energy source
[Ref.: #65498]	L-arginine ChEBI	-	carbon source
[Ref.: #65498]	L-arginine ChEBI	-	electron acceptor
[Ref.: #65498]	L-arginine ChEBI	-	energy source
[Ref.: #65498]	L-aspartate ChEBI	-	carbon source
[Ref.: #65498]	L-aspartate ChEBI	-	energy source
[Ref.: #65498]	L-glutamine ChEBI	-	carbon source
[Ref.: #65498]	L-glutamine ChEBI	-	energy source
[Ref.: #65498]	L-lysine ChEBI	+	carbon source
[Ref.: #65498]	L-lysine ChEBI	+	energy source
[Ref.: #65498]	L-serine ChEBI	-	carbon source
[Ref.: #65498]	L-serine ChEBI	-	energy source
[Ref.: #65498]	L-tryptophan ChEBI	-	carbon source
[Ref.: #65498]	L-tryptophan ChEBI	-	energy source
[Ref.: #65498]	malate ChEBI	-	carbon source
[Ref.: #65498]	malate ChEBI	-	energy source
[Ref.: #65498]	maltose ChEBI	-	builds acid from
[Ref.: #65498]	maltose ChEBI	+	carbon source
[Ref.: #65498]	maltose ChEBI	+	energy source
[Ref.: #65498]	nitrate ChEBI	-	electron acceptor
[Ref.: #65498]	nitrate ChEBI	-	reduction
[Ref.: #65498]	propionate ChEBI	+	carbon source
[Ref.: #65498]	propionate ChEBI	+	energy source
[Ref.: #65498]	raffinose ChEBI	-	carbon source
[Ref.: #65498]	raffinose ChEBI	-	energy source
[Ref.: #65498]	ribitol ChEBI	-	carbon source
[Ref.: #65498]	ribitol ChEBI	-	energy source
[Ref.: #65498]	starch ChEBI	-	hydrolysis
[Ref.: #65498]	succinate ChEBI	+	carbon source
[Ref.: #65498]	succinate ChEBI	+	energy source
[Ref.: #65498]	sucrose ChEBI	-	carbon source
[Ref.: #65498]	sucrose ChEBI	-	energy source
[Ref.: #65498]	tween 20 ChEBI	-	hydrolysis
[Ref.: #65498]	tween 40 ChEBI	-	hydrolysis
[Ref.: #65498]	tween 80 ChEBI	-	hydrolysis
[Ref.: #65498]	xylitol ChEBI	-	carbon source
[Ref.: #65498]	xylitol ChEBI	-	energy source
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Concentration		Antibiotic resistance	Concentration
[Ref.: #65498]	(-)-anisomycin ChEBI	yes	30	µg (disc)
[Ref.: #65498]	ampicillin ChEBI				yes	10	µg (disc)
[Ref.: #65498]	bacitracin ChEBI	yes	0.04	Unit (disc)
[Ref.: #65498]	chloramphenicol ChEBI				yes	10	µg (disc)
[Ref.: #65498]	erythromycin ChEBI	yes	10	µg (disc)
[Ref.: #65498]	gentamicin ChEBI				yes	10	µg (disc)
[Ref.: #65498]	kanamycin ChEBI				yes	30	µg (disc)
[Ref.: #65498]	neomycin ChEBI				yes	30	µg (disc)
[Ref.: #65498]	novobiocin ChEBI	yes	30	µg (disc)
[Ref.: #65498]	penicillin ChEBI				yes	10	Unit (disc)
[Ref.: #65498]	rifampicin ChEBI				yes	10	µg (disc)
[Ref.: #65498]	streptomycin ChEBI				yes	10	µg (disc)
[Ref.: #65498]	tetracycline ChEBI				yes	30	µg (disc)
	Only first 10 entries are displayed. Click here to see all.

	Antibiotica	Group ID of combined antibiotics	Metabolite	Antibiotic resistance	Concentration
[Ref.: #65498]		26	sulfamethoxazole ChEBI	yes	23.75	µg (disc)
[Ref.: #65498]		26	trimethoprim ChEBI	yes	1.25	µg (disc)

	Metabolite production	Metabolite	Production
[Ref.: #65498]		hydrogen sulfide ChEBI	no
[Ref.: #65498]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #65498]	alkaline phosphatase	-	3.1.3.1
[Ref.: #65498]	catalase	+	1.11.1.6
[Ref.: #65498]	cytochrome oxidase	+	1.9.3.1
[Ref.: #65498]	lysine decarboxylase	-	4.1.1.18
[Ref.: #65498]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #65498]	urease	-	3.5.1.5

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #65498]	Sample type/isolated from	brine sample from a solar saltern
[Ref.: #65498]	Geographic location (country and/or sea, region)	Gomso-ri, Buan
[Ref.: #65498]	Country	Republic of Korea
[Ref.: #65498]	Country ISO 3 Code	KOR
[Ref.: #65498]	Continent	Asia
[Ref.: #65498]	Geographic location	35.5833°/126.6000°
[Ref.: #65498]	Enrichment culture	modified-SG medium
[Ref.: #65498]	Enrichment culture composition	Modified-SG medium which contained the following ingredients (per litre): 7.5g casamino acids, 10g yeast extract, 3g trisodium citrate, 20g MgSO4 . 7H2O, 2g KCl, 0.05g FeSO4 . 7H2O, 200g NaCl and 0.1g NH4Cl. The pH was adjusted to 7.5 with 1 M NaOH solution.
[Ref.: #65498]	Enrichment culture duration	2 weeks
[Ref.: #65498]	Enrichment culture temperature	37 ̊C
[Ref.: #65498]	Isolation/enrichment procedure	After three subcultures, the enrichment culture was plated onto modified-SG agar plates (1.6 % (w/v) agar). The plates were incubated in the dark at 37 °C for at least 2 weeks under aerobic conditions.

[Ref.: #67770]	Sample type/isolated from	Gomso solar saltern
[Ref.: #67770]	Country	Republic of Korea
[Ref.: #67770]	Country ISO 3 Code	KOR
[Ref.: #67770]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Engineered	#Other	#Mine
#Condition	#Saline	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence KX151776 (>99% sequence identity) for Haloplanus from Microbeatlas

Aquatic Samples	25
Soil Samples	1
Animal Samples
Plant Samples
Total Samples	26

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #65498]	16S rRNA gene sequence	KX151776		GenBank

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Haloplanus salinarum JCM 31424	GCA_024498175	complete	GenBank	1912324 tax ID	*

[Ref.: #65498]	GC-content	66.2 mol%	fluorimetric
[Ref.: #67770]	GC-content	66.2 mol%	thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Haloplanus salinarum JCM 31424 NCBI: GCA_024498175.1
[Ref.: #69480]	flagellated	no	96.136	no
[Ref.: #69480]	gram-positive	no	97.659	yes
[Ref.: #69480]	anaerobic	no	94.789	no
[Ref.: #69480]	aerobic	yes	82.19	yes
[Ref.: #69480]	halophile	yes	93.91	no
[Ref.: #69480]	spore-forming	no	95.413	no
[Ref.: #69480]	motile	yes	53.294	yes
[Ref.: #69480]	glucose-ferment	no	90.174	no
[Ref.: #69480]	thermophile	no	82.008	no
[Ref.: #69480]	glucose-util	yes	79.198	no

Availability in culture collections External links

[Ref.: #65498]

Culture collection no.

JCM 31424, KCCM 43210

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Haloplanus salinarum sp. nov., an extremely halophilic archaeon isolated from a solar saltern.	Hwang HB, Kim YE, Koh HW, Song HS, Roh SW, Kim SJ, Nam SW, Park SJ	Int J Syst Evol Microbiol	10.1099/ijsem.0.002313	2017	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#65498

Han-Bit Hwang, Ye-Eun Kim, Hyeon-Woo Koh, Hye Seon Song, Seong Woon Roh, So-Jeong Kim, Seung Won Nam and Soo-Je Park: Haloplanus salinarum sp. nov., an extremely halophilic archaeon isolated from a solar saltern. IJSEM 67: 4456 - 4461 2017 ( DOI 10.1099/ijsem.0.002313 , PubMed 28945530 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

* These data were automatically processed and therefore are not curated

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Haloplanus salinarum JCM 31424

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