Name: Epidermidibacterium keratini EPI-7
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 158621

Type strain:

Species: Epidermidibacterium keratini

Strain Designation: EPI-7

Culture col. no.: KCCM 90264, JCM 31644

Strain history: D.-G. Lee; COSMAX R&I Center, South Korea; EPI-7.

NCBI tax ID(s): 1891644 (species)

Section

Epidermidibacterium keratini EPI-7 is an aerobe, chemoheterotroph, Gram-positive bacterium that forms circular colonies and was isolated from human skin .

colony-forming
Gram-positive
rod-shaped
aerobe
chemoheterotroph
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Geodermatophilales*
	Family	*Antricoccaceae*
	Genus	*Epidermidibacterium*
	Species	**Epidermidibacterium keratini**
	Full Scientific Name (LPSN)	Epidermidibacterium keratini Lee et al. 2018

Information on morphological and physiological properties Morphology

[Ref.: #65346]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #65346]	Cell length	0.7-1 µm

[Ref.: #65346]	Cell width	0.3-0.5 µm

[Ref.: #65346]	Cell shape	rod-shaped

[Ref.: #65346]	Motility	no
[Ref.: #69480]	Motility	no Confidence in %90.93

[Ref.: #65346]	Colony color	pale yellow
[Ref.: #65346]	Colony shape	circular
[Ref.: #65346]	Cultivation medium used	R2A

Information on culture and growth conditions Culture and growth conditions

[Ref.: #65346]

Culture medium

Reasoner's 2A agar (R2A)

[Ref.: #65346]

Culture medium growth

[Ref.: #65346]

Culture medium

Nutrient agar (NA)

[Ref.: #65346]

Culture medium growth

[Ref.: #65346]

Culture medium

ISP2

[Ref.: #65346]

Culture medium growth

[Ref.: #65346]

Culture medium

tryptic soy agar

[Ref.: #65346]

Culture medium growth

	Kind of temperature		Temperature
[Ref.: #65346]		growth	15-35 °C
[Ref.: #65346]		optimum	25 °C
[Ref.: #65346]	no	growth	10 °C
[Ref.: #65346]	no	growth	40 °C
[Ref.: #67770]		growth	28 °C

[Ref.: #65346]	Temperature range	mesophilic
[Ref.: #65346]	Temperature range	psychrophilic
[Ref.: #65346]	Temperature range	thermophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #65346]			optimum	6
[Ref.: #65346]			growth	5-8.5

Information on physiology and metabolism Physiology and metabolism

[Ref.: #65346]

Oxygen tolerance

aerobe

[Ref.: #65346]

Nutrition type

chemoheterotroph

[Ref.: #65346]	Ability of spore formation	no
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.758
[Ref.: #69481]	Ability of spore formation	no Confidence in %96

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #65346]		NaCl		growth	0-10	%
[Ref.: #65346]		NaCl		optimum	0	%

[Ref.: #67770]

Observation

quinones: MK-9(H₄)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #65346]	(S)-lactate ChEBI	+	assimilation
[Ref.: #65346]	2-deoxyadenosine ChEBI	+	assimilation
[Ref.: #65346]	2-hydroxybutyrate ChEBI	+	assimilation
[Ref.: #65346]	2-oxoglutarate ChEBI	+	assimilation
[Ref.: #65346]	2-oxopentanoate ChEBI	+	assimilation
[Ref.: #65346]	3-hydroxybutyrate ChEBI	+	assimilation
[Ref.: #65346]	4-hydroxybutyrate ChEBI	+	assimilation
[Ref.: #65346]	4-hydroxyphenylacetic acid ChEBI	+	assimilation
[Ref.: #65346]	acetate ChEBI	-	assimilation
[Ref.: #65346]	adenosine ChEBI	+	assimilation
[Ref.: #65346]	alpha-D-glucose ChEBI	+	assimilation
[Ref.: #65346]	alpha-D-glucose 6-phosphate ChEBI	-	assimilation
[Ref.: #65346]	amp ChEBI	+	assimilation
[Ref.: #65346]	arbutin ChEBI	-	assimilation
[Ref.: #65346]	bromosuccinate ChEBI	+	assimilation
[Ref.: #65346]	carboxymethylcellulose ChEBI	-	degradation
[Ref.: #65346]	casein	+	degradation
[Ref.: #65346]	cellobiose ChEBI	-	assimilation
[Ref.: #65346]	citrate ChEBI	+	assimilation
[Ref.: #65346]	D-alanine ChEBI	-	assimilation
[Ref.: #65346]	D-aspartate ChEBI	-	assimilation
[Ref.: #65346]	D-fructose ChEBI	+	assimilation
[Ref.: #65346]	D-fucose ChEBI	+	assimilation
[Ref.: #65346]	D-galactose ChEBI	+	assimilation
[Ref.: #65346]	D-galacturonic acid ChEBI	-	assimilation
[Ref.: #65346]	D-glucose 6-phosphate ChEBI	-	assimilation
[Ref.: #65346]	D-glucuronate ChEBI	-	assimilation
[Ref.: #65346]	D-malate ChEBI	-	assimilation
[Ref.: #65346]	D-mannitol ChEBI	-	assimilation
[Ref.: #65346]	D-mannose ChEBI	+	assimilation
[Ref.: #65346]	D-psicose ChEBI	+	assimilation
[Ref.: #65346]	D-ribose ChEBI	+	assimilation
[Ref.: #65346]	D-serine ChEBI	+	assimilation
[Ref.: #65346]	D-sorbitol ChEBI	-	assimilation
[Ref.: #65346]	D-trehalose ChEBI	-	assimilation
[Ref.: #65346]	dextrin ChEBI	+	assimilation
[Ref.: #65346]	dna ChEBI	-	degradation
[Ref.: #65346]	formate ChEBI	-	assimilation
[Ref.: #65346]	galactarate ChEBI	-	assimilation
[Ref.: #65346]	gamma-aminobutyric acid ChEBI	-	assimilation
[Ref.: #65346]	gelatin ChEBI	-	assimilation
[Ref.: #65346]	gentiobiose ChEBI	-	assimilation
[Ref.: #65346]	glucuronamide ChEBI	-	assimilation
[Ref.: #65346]	glycerol ChEBI	-	assimilation
[Ref.: #65346]	glycerol 1-phosphate ChEBI	-	assimilation
[Ref.: #65346]	glycine-proline ChEBI	-	assimilation
[Ref.: #65346]	inosine ChEBI	+	assimilation
[Ref.: #65346]	L-arabinose ChEBI	-	assimilation
[Ref.: #65346]	L-aspartate ChEBI	-	assimilation
[Ref.: #65346]	L-fucose ChEBI	-	assimilation
[Ref.: #65346]	L-galactonic acid gamma-lactone ChEBI	-	assimilation
[Ref.: #65346]	L-histidine ChEBI	-	assimilation
[Ref.: #65346]	L-malate ChEBI	-	assimilation
[Ref.: #65346]	L-pyroglutamic acid ChEBI	-	assimilation
[Ref.: #65346]	L-rhamnose ChEBI	-	assimilation
[Ref.: #65346]	L-serine ChEBI	+	assimilation
[Ref.: #65346]	lactamide ChEBI	+	assimilation
[Ref.: #65346]	maltose ChEBI	-	assimilation
[Ref.: #65346]	maltotriose ChEBI	+	assimilation
[Ref.: #65346]	melibiose ChEBI	-	assimilation
[Ref.: #65346]	methyl (R)-lactate ChEBI	+	assimilation
[Ref.: #65346]	methyl alpha-D-galactoside ChEBI	-	assimilation
[Ref.: #65346]	methyl alpha-D-glucopyranoside ChEBI	-	assimilation
[Ref.: #65346]	methyl beta-D-galactoside ChEBI	-	assimilation
[Ref.: #65346]	methyl beta-D-glucopyranoside ChEBI	-	assimilation
[Ref.: #65346]	methyl pyruvate ChEBI	+	assimilation
[Ref.: #65346]	myo-inositol ChEBI	-	assimilation
[Ref.: #65346]	N-acetyl-D-mannosamine ChEBI	+	assimilation
[Ref.: #65346]	N-acetylglucosamine ChEBI	+	assimilation
[Ref.: #65346]	nitrate ChEBI	+	reduction
[Ref.: #65346]	pectin ChEBI	+	assimilation
[Ref.: #65346]	propionate ChEBI	+	assimilation
[Ref.: #65346]	pyruvate ChEBI	+	assimilation
[Ref.: #65346]	quinate ChEBI	-	assimilation
[Ref.: #65346]	raffinose ChEBI	-	assimilation
[Ref.: #65346]	starch ChEBI	+	degradation
[Ref.: #65346]	sucrose ChEBI	-	assimilation
[Ref.: #65346]	thymidine ChEBI	+	assimilation
[Ref.: #65346]	turanose ChEBI	-	assimilation
[Ref.: #65346]	tween 80 ChEBI	+	assimilation
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #65346]	acid phosphatase	+	3.1.3.2
[Ref.: #65346]	alkaline phosphatase	+	3.1.3.1
[Ref.: #65346]	alpha-chymotrypsin	+	3.4.21.1
[Ref.: #65346]	alpha-fucosidase	-	3.2.1.51
[Ref.: #65346]	alpha-galactosidase	-	3.2.1.22
[Ref.: #65346]	alpha-glucosidase	-	3.2.1.20
[Ref.: #65346]	alpha-mannosidase	-	3.2.1.24
[Ref.: #65346]	beta-glucosidase	-	3.2.1.21
[Ref.: #65346]	beta-glucuronidase	-	3.2.1.31
[Ref.: #65346]	catalase	-	1.11.1.6
[Ref.: #65346]	cystine arylamidase	+	3.4.11.3
[Ref.: #65346]	cytochrome oxidase	-	1.9.3.1
[Ref.: #65346]	esterase (C 4)	+
[Ref.: #65346]	esterase Lipase (C 8)	+
[Ref.: #65346]	leucine arylamidase	+	3.4.11.1
[Ref.: #65346]	lipase (C 14)	-
[Ref.: #65346]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #65346]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #65346]	trypsin	-	3.4.21.4
[Ref.: #65346]	valine arylamidase	+
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #65346]

Fatty acid	Percentage
C17:1ω8c	19.9
C16:0	18.4
C15:0 iso	10.1
C16:1ω6c and/or C16:1ω7c	10
C16:0 iso	8.5
C17:0	7.1
C16:1ω9c	3.2
C15:0 anteiso	3.2
C15:1ω8c	2.6
C18:1ω9c	2.5
C17:0 anteiso	2.1
C14:0 iso	1.9
C14:0	1.6
C18:1ω6c	1.2
C15:1ω6c	1.1
C18:0	1.1
C16:1 iso H	1

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #65346]	Sample type/isolated from	human skin (keratinocytes)
[Ref.: #65346]	Geographic location (country and/or sea, region)	Seong-nam City
[Ref.: #65346]	Country	Republic of Korea
[Ref.: #65346]	Country ISO 3 Code	KOR
[Ref.: #65346]	Continent	Asia
[Ref.: #65346]	Enrichment culture	R2A
[Ref.: #65346]	Enrichment culture duration	3 days
[Ref.: #65346]	Enrichment culture temperature	28 ̊C
[Ref.: #65346]	Isolation/enrichment procedure	The human epidermal keratinocytes sampled had a pH of 6.5 and a total aerobic microbial cell count of 6.4 x 10^3 c.f.u. ml-1. For isolation, serially diluted human epidermal keratinocyte samples were spread onto the following solid media (all from Difco): nutrient, R2A, ISP 2 and trypticase soy agars. The plates were incubated at 28°C for 3 days, after which single colonies were formed on R2A plates.

[Ref.: #67770]	Sample type/isolated from	Human skin (keratinocytes)
[Ref.: #67770]	Geographic location (country and/or sea, region)	Seong-nam City
[Ref.: #67770]	Country	Republic of Korea
[Ref.: #67770]	Country ISO 3 Code	KOR
[Ref.: #67770]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Host Body-Site	#Organ	#Skin, Nail, Hair
#Host	#Human	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence KX755247 (>99% sequence identity) for Actinomycetia from Microbeatlas

Aquatic Samples	250
Soil Samples	380
Animal Samples	1178
Plant Samples	32
Total Samples	1840

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #65346]	16S rRNA gene sequence	KX755247		GenBank

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Epidermidibacterium keratini EPI-7	GCA_009834025	complete	GenBank	1891644 tax ID	*
[Ref.: #66792]	Epidermidibacterium keratini EPI-7	2883308892	complete	JGI IMG/M	1891644 tax ID	*

[Ref.: #67770]

GC-content

68.9 mol%

high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Epidermidibacterium keratini EPI-7 NCBI: GCA_009834025.1
[Ref.: #69481]	spore-forming	no	96	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Epidermidibacterium keratini EPI-7 NCBI: GCA_009834025.1
[Ref.: #69480]	motile	no	93.053	no
[Ref.: #69480]	flagellated	no	98.506	yes
[Ref.: #69480]	gram-positive	yes	92.052	no
[Ref.: #69480]	anaerobic	no	98.759	yes
[Ref.: #69480]	aerobic	yes	93.885	no
[Ref.: #69480]	halophile	no	81.843	no
[Ref.: #69480]	spore-forming	no	89.796	yes
[Ref.: #69480]	glucose-util	yes	86.959	yes
[Ref.: #69480]	thermophile	no	98.547	no
[Ref.: #69480]	glucose-ferment	no	92.605	no

Availability in culture collections External links

[Ref.: #65346]

Culture collection no.

KCCM 90264, JCM 31644

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Epidermidibacterium keratini gen. nov., sp. nov., a member of the family Sporichthyaceae, isolated from keratin epidermis.	Lee DG, Trujillo ME, Kang S, Nam JJ, Kim YJ	Int J Syst Evol Microbiol	10.1099/ijsem.0.002579	2018	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#65346

Dong-Geol Lee, Martha E. Trujillo, Seunghyun Kang, Jin-Ju Nam, Yeon-Jun Kim: Epidermidibacterium keratini gen. nov., sp. nov., a member of the family Sporichthyaceae, isolated from keratin epidermis. IJSEM 68: 745 - 750 2018 ( DOI 10.1099/ijsem.0.002579 , PubMed 29458473 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Epidermidibacterium keratini in external resources:
SILVA
BRENDA
PANGAEA
StrainInfo
GBIF