Polynucleobacter hirudinilacicola (DSM 23911, LMG 30144, MWH-EgelM1-30-B4)

Name: Polynucleobacter hirudinilacicola (DSM 23911, LMG 30144, MWH-EgelM1-30-B4)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 158406

Type strain

Strain Designation MWH-EgelM1-30-B4

Culture col. no. DSM 23911 LMG 30144 MWH-EgelM1-30-B4

NCBI tax ID(s) 1743166

Special Collections DSMZ Literature strains

Links

Polynucleobacter hirudinilacicola MWH-EgelM1-30-B4 is an aerobe, chemoorganoheterotroph, psychrophilic prokaryote that forms circular colonies and was isolated from freshwater.

rod-shaped colony-forming aerobe chemoorganoheterotroph psychrophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 403346

LMG 30144,DSM 23911

@ref 20215

Last LPSN update 2025-12-16 10:08:01

Domain Pseudomonadati

Phylum Pseudomonadota

Class Betaproteobacteria

Order Burkholderiales

Family Burkholderiaceae

Genus Polynucleobacter

Species Polynucleobacter hirudinilacicola

Full scientific name Polynucleobacter hirudinilacicola Hahn et al. 2018

Morphology

Cell morphology

@ref	Cell length	Cell width	Cell shape
66620	0.5-1.2 µm	0.3-0.5 µm	rod-shaped
125439

Colony morphology

@ref	Colony color	Colony shape	Medium used
66620	non-pigmented	circular	nutrient broth soytone yeast extract agar

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
65082	NSY-MEDIUM FOR POLYNUCLEOBACTER (DSMZ Medium 1167)		Medium recipe at MediaDive	Name: NSY-MEDIUM FOR POLYNUCLEOBACTER (DSMZ Medium 1167) Composition: Soy peptone 1.0 g/l Nutrient broth 1.0 g/l Yeast extract 1.0 g/l MgSO4 x 7 H2O 0.075 g/l Ca(NO3)2 x 4 H2O 0.043 g/l NaHCO3 0.016 g/l KCl 0.005 g/l K2HPO4 x 3 H2O 0.0037 g/l Na2-EDTA 5e-05 g/l H3BO3 3e-05 g/l CoCl2 x 6 H2O 2e-05 g/l FeSO4 x 7 H2O 2e-05 g/l ZnSO4 x 7 H2O 1e-05 g/l MnCl2 x 4 H2O 3e-06 g/l Na2MoO4 x 2 H2O 3e-06 g/l NiCl2 x 6 H2O 2e-06 g/l CuCl2 x 2 H2O 1e-06 g/l Tap water Distilled water
65082	R2A MEDIUM (DSMZ Medium 830)		Medium recipe at MediaDive	Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
65082	positive	growth	22	psychrophilic
65082	positive	growth	28	mesophilic
66620	positive	growth	5-31

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance
66620	aerobe
125439	obligate aerobe

Nutrition type

66620

Typechemoorganoheterotroph

Spore formation

@ref	Spore formation	Confidence
125439		99.8

Halophily

@ref	Salt	Growth	Tested relation	Concentration
66620	NaCl	positive	growth	0-0.1 %(w/v)
66620	NaCl		growth	>0.2 %(w/v)

Observation

66620

ObservationContains free-living Polynucleobacter strains dwelling in the water body of alkaline and probably also circum-neutral freshwater systems

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
66620	39150 ChEBI	4-oxopentanoate	-	assimilation
66620	16411 ChEBI	acetic acid	+	assimilation
66620	17750 ChEBI	betaine	-	assimilation
66620	53258 ChEBI	citric acid	-	assimilation
66620	15824 ChEBI	D-fructose	-	assimilation
66620	12936 ChEBI	D-galactose	-	assimilation
66620	18024 ChEBI	D-galacturonic acid	-	assimilation
66620	17634 ChEBI	D-glucose	-	assimilation
66620	62318 ChEBI	D-lyxose	-	assimilation
66620	16024 ChEBI	D-mannose	-	assimilation
66620	17924 ChEBI	D-sorbitol	-	assimilation
66620	29806 ChEBI	fumarate	+	assimilation
66620	29805 ChEBI	glycolate	-	assimilation
66620	36655 ChEBI	glyoxylate	-	assimilation
66620	16977 ChEBI	L-alanine	+	assimilation
66620	17196 ChEBI	L-asparagine	-	assimilation
66620	29991 ChEBI	L-aspartate	-	assimilation
66620	17561 ChEBI	L-cysteine	+	assimilation
66620	18287 ChEBI	L-fucose	-	assimilation
66620	29985 ChEBI	L-glutamate	-	assimilation
66620	15971 ChEBI	L-histidine	-	assimilation
66620	15603 ChEBI	L-leucine	-	assimilation
66620	25115 ChEBI	malate	-	assimilation
66620	15792 ChEBI	malonate	-	assimilation
66620	30623 ChEBI	oxalate	-	assimilation
66620	16452 ChEBI	oxaloacetate	+	assimilation
66620	17272 ChEBI	propionate	-	assimilation
66620	15361 ChEBI	pyruvate	+	assimilation
66620	30031 ChEBI	succinate	+	assimilation

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Freshwater

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
65082	freshwater	Lake Egelsee (near Mattsee)	Austria	AUT	Europe
66620	watersamples from the water columns of freshwater systems	Lake Egelsee	Austria	AUT	Europe

Taxonmaps

69479

Global distribution of 16S sequence FN429662 (>99% sequence identity) for Polynucleobacter from Microbeatlas

Aquatic Samples	34169
Soil Samples	1815
Animal Samples	6310
Plant Samples	555
Total Samples	42849

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
65082	1	Risk group (German classification)

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM219253v1 assembly for Polynucleobacter hirudinilacicola MWH-EgelM1-30-B4	contig	GCA_002192535	1743166.3	2590828818	1743166	70.08

Genome browser: GCA_002192535

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
66620	Polynucleobacter necessarius subsp. asymbioticus partial 16S rRNA gene and ITS1, strain MWH-EgelM1-30-B4	FN429662	2006		1743166

GC content

@ref	GC-content (mol%)	Method
66620	45.3	genome sequence analysis

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Polynucleobacter hirudinilacicola MWH-EgelM1-30-B4
NCBI: GCA_002192535

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	99.80	no
125439	motility	BacteriaNetⓘ	yes	81.30	no
125439	gram_stain	BacteriaNetⓘ	negative	99.80	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	98.50	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Polynucleobacter hirudinilacicola sp. nov. and Polynucleobacter campilacus sp. nov., both isolated from freshwater systems.	Hahn MW, Koll U, Schmidt J, Huymann LR, Karbon G, Lang E	Int J Syst Evol Microbiol	10.1099/ijsem.0.002880	2018

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#65082	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 23911
#66620	Martin W. Hahn, Ulrike Koll, Johanna Schmidt, Lesley R. Huymann, Gerlinde Karbon, Elke Lang: Polynucleobacter hirudinilacicola sp. nov. and Polynucleobacter campilacus sp. nov., both isolated from freshwater systems. IJSEM 68: 2593 - 2601 2018 ( DOI 10.1099/ijsem.0.002880 , PubMed 29939120 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Polynucleobacter hirudinilacicola (DSM 23911, LMG 30144, MWH-EgelM1-30-B4)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Nutrition type

Spore formation

Halophily

Observation

Metabolite utilization

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_002192535

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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