Name: Polynucleobacter hirudinilacicola MWH-EgelM1-30-B4
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 158406

Type strain:

Species: Polynucleobacter hirudinilacicola

Strain Designation: MWH-EgelM1-30-B4

Culture col. no.: DSM 23911, LMG 30144

Strain history: <- M. W. Hahn, Austrian Academy of Sci., Inst. Limnology; MWH-EgelM1-30-B4 <-

NCBI tax ID(s): 1743166 (species)

SI-ID 403346

DSM 23911

Special Collections

Section

Polynucleobacter hirudinilacicola MWH-EgelM1-30-B4 is an aerobe, chemoorganoheterotroph, rod-shaped bacterium that forms circular colonies and was isolated from freshwater.

colony-forming
rod-shaped
aerobe
chemoorganoheterotroph
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Betaproteobacteria*
	Order	*Burkholderiales*
	Family	*Burkholderiaceae*
	Genus	*Polynucleobacter*
	Species	**Polynucleobacter hirudinilacicola**
	Full Scientific Name (LPSN)	Polynucleobacter hirudinilacicola Hahn et al. 2018

Information on morphological and physiological properties Morphology

[Ref.: #66620]	Cell length	0.5-1.2 µm

[Ref.: #66620]	Cell width	0.3-0.5 µm

[Ref.: #66620]	Cell shape	rod-shaped

[Ref.: #66620]	Colony color	non-pigmented
[Ref.: #66620]	Colony shape	circular
[Ref.: #66620]	Cultivation medium used	nutrient broth soytone yeast extract agar

Information on culture and growth conditions Culture and growth conditions

[Ref.: #65082]

Culture medium

NSY-MEDIUM FOR POLYNUCLEOBACTER (DSMZ Medium 1167)

[Ref.: #65082]

Culture medium growth

[Ref.: #65082]

Culture medium link

Medium recipe at MediaDive

[Ref.: #65082]

Culture medium composition

expand / minimize

[Ref.: #65082]

Culture medium

R2A MEDIUM (DSMZ Medium 830)

[Ref.: #65082]

Culture medium growth

[Ref.: #65082]

Culture medium link

Medium recipe at MediaDive

[Ref.: #65082]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #65082]	growth	22 °C
[Ref.: #65082]	growth	28 °C
[Ref.: #66620]	growth	5-31 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #66620]

Oxygen tolerance

aerobe

[Ref.: #66620]

Nutrition type

chemoorganoheterotroph

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #66620]		NaCl		growth	0-0.1	%(w/v)
[Ref.: #66620]		NaCl	no	growth	>0.2	%(w/v)

[Ref.: #66620]

Observation

Contains free-living Polynucleobacter strains dwelling in the water body of alkaline and probably also circum-neutral freshwater systems

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #66620]	4-oxopentanoate ChEBI	-	assimilation
[Ref.: #66620]	acetic acid ChEBI	+	assimilation
[Ref.: #66620]	betaine ChEBI	-	assimilation
[Ref.: #66620]	citric acid ChEBI	-	assimilation
[Ref.: #66620]	D-fructose ChEBI	-	assimilation
[Ref.: #66620]	D-galactose ChEBI	-	assimilation
[Ref.: #66620]	D-galacturonic acid ChEBI	-	assimilation
[Ref.: #66620]	D-glucose ChEBI	-	assimilation
[Ref.: #66620]	D-lyxose ChEBI	-	assimilation
[Ref.: #66620]	D-mannose ChEBI	-	assimilation
[Ref.: #66620]	D-sorbitol ChEBI	-	assimilation
[Ref.: #66620]	fumarate ChEBI	+	assimilation
[Ref.: #66620]	glycolate ChEBI	-	assimilation
[Ref.: #66620]	glyoxylate ChEBI	-	assimilation
[Ref.: #66620]	L-alanine ChEBI	+	assimilation
[Ref.: #66620]	L-asparagine ChEBI	-	assimilation
[Ref.: #66620]	L-aspartate ChEBI	-	assimilation
[Ref.: #66620]	L-cysteine ChEBI	+	assimilation
[Ref.: #66620]	L-fucose ChEBI	-	assimilation
[Ref.: #66620]	L-glutamate ChEBI	-	assimilation
[Ref.: #66620]	L-histidine ChEBI	-	assimilation
[Ref.: #66620]	L-leucine ChEBI	-	assimilation
[Ref.: #66620]	malate ChEBI	-	assimilation
[Ref.: #66620]	malonate ChEBI	-	assimilation
[Ref.: #66620]	oxalate ChEBI	-	assimilation
[Ref.: #66620]	oxaloacetate ChEBI	+	assimilation
[Ref.: #66620]	propionate ChEBI	-	assimilation
[Ref.: #66620]	pyruvate ChEBI	+	assimilation
[Ref.: #66620]	succinate ChEBI	+	assimilation
	Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #65082]	Sample type/isolated from	freshwater
[Ref.: #65082]	Geographic location (country and/or sea, region)	Lake Egelsee (near Mattsee)
[Ref.: #65082]	Country	Austria
[Ref.: #65082]	Country ISO 3 Code	AUT
[Ref.: #65082]	Continent	Europe

[Ref.: #66620]	Sample type/isolated from	watersamples from the water columns of freshwater systems
[Ref.: #66620]	Geographic location (country and/or sea, region)	Lake Egelsee
[Ref.: #66620]	Country	Austria
[Ref.: #66620]	Country ISO 3 Code	AUT
[Ref.: #66620]	Continent	Europe
[Ref.: #66620]	Geographic location	47.9660°/13.1250°
[Ref.: #66620]	Enrichment culture	nutrient broth soytone yeast extract medium
[Ref.: #66620]	Enrichment culture composition	2006-04-21
[Ref.: #66620]	Isolation/enrichment procedure	filtration acclimation method
* marker position based on {}

Isolation sources categories

#Environmental

#Aquatic

#Freshwater

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence FN429662 (>99% sequence identity) for Polynucleobacter from Microbeatlas

Aquatic Samples	34169
Soil Samples	1815
Animal Samples	6310
Plant Samples	555
Total Samples	42849

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #65082]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #66620]	Polynucleobacter necessarius subsp. asymbioticus partial 16S rRNA gene and ITS1, strain MWH-EgelM1-30-B4	FN429662	2006	GenBank ENA	1743166 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Polynucleobacter hirudinilacicola MWH-EgelM1-30-B4	GCA_002192535	contig	GenBank ENA	1743166 tax ID	*
[Ref.: #66792]	Polynucleobacter hirudinilacicola MWH-EgelM1-30-B4	2590828818	complete	JGI IMG/M	1743166 tax ID	*

[Ref.: #66620]

GC-content

45.3 mol%

genome sequence analysis

Availability in culture collections External links

[Ref.: #65082]

Culture collection no.

DSM 23911, LMG 30144

[Ref.: #111209]

SI-ID 403346

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Polynucleobacter hirudinilacicola sp. nov. and Polynucleobacter campilacus sp. nov., both isolated from freshwater systems.	Hahn MW, Koll U, Schmidt J, Huymann LR, Karbon G, Lang E	Int J Syst Evol Microbiol	10.1099/ijsem.0.002880	2018	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#65082

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 23911

#66620

Martin W. Hahn, Ulrike Koll, Johanna Schmidt, Lesley R. Huymann, Gerlinde Karbon, Elke Lang: Polynucleobacter hirudinilacicola sp. nov. and Polynucleobacter campilacus sp. nov., both isolated from freshwater systems. IJSEM 68: 2593 - 2601 2018 ( DOI 10.1099/ijsem.0.002880 , PubMed 29939120 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#111209

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID403346.1 )

* These data were automatically processed and therefore are not curated

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