Aminipila butyrica (DSM 103574, JCM 31555, FH042)

Name: Aminipila butyrica (DSM 103574, JCM 31555, FH042)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 158376

Type strain

Strain Designation FH042

Culture col. no. DSM 103574 JCM 31555 FH042

NCBI tax ID(s) 433296

Special Collections DSMZ JCM Literature strains

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Aminipila butyrica FH042 is a mesophilic, Gram-positive, motile prokaryote that was isolated from Methanogenic reactor of cattlefarms.

Gram-positive motile rod-shaped mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 396548

JCM 31555,DSM 103574

@ref 20215

Last LPSN update 2025-12-16 10:08:00

Domain Bacillati

Phylum Bacillota

Class Clostridia

Order Peptostreptococcales

Family Anaerovoracaceae

Genus Aminipila

Species Aminipila butyrica

Full scientific name Aminipila butyrica Ueki et al. 2018

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Motility	Flagellum arrangement
65320	positive	3-8 µm	0.7-1 µm	rod-shaped		polar

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
65052	PY + X MEDIUM (N2/CO2) (DSMZ Medium 104c)	Medium recipe at MediaDive	Name: PY + X MEDIUM (N2/CO2) (DSMZ Medium 104c; with strain-specific modifications) Composition: Yeast extract 10.0 g/l Trypticase peptone 5.0 g/l Meat peptone 5.0 g/l Casamino acids 3.0 g/l Na2CO3 1.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l KH2PO4 0.04 g/l K2HPO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Sodium resazurin 0.0005 g/l Distilled water
65320	PY4S
65320	chopped meat broth

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
65052	positive	growth	30	mesophilic
65320	positive	growth	10-35
65320	positive	optimum	30	mesophilic
67770	positive	growth	30	mesophilic

Culture pH

@ref	Ability	Type	PH
65320	positive	optimum	6.1-6.8
65320	positive	growth	5.3-8.2

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
125438	anaerobe	92.073
125439	anaerobe	99

Spore formation

65320

Spore formationno

Halophily

@ref	Salt	Growth	Tested relation	Concentration
65320	NaCl	positive	growth	0-20 g/L
65320	NaCl	positive	optimum	0 g/L

Observation

@ref	Observation
65320	Longer rods up to 40 µm often occur.
65320	B-vitamins are not required for growth.

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
65320	27613 ChEBI	amygdalin	-	assimilation
65320	22599 ChEBI	arabinose	-	assimilation
65320	85146 ChEBI	carboxymethylcellulose	-	assimilation
65320		casamino acids	+	assimilation
65320	17057 ChEBI	cellobiose	-	assimilation
65320	62968 ChEBI	cellulose	-	assimilation
65320	23652 ChEBI	dextrin	-	assimilation
65320	4853 ChEBI	esculin	-	assimilation
65320	4853 ChEBI	esculin	-	hydrolysis
65320	28757 ChEBI	fructose	-	assimilation
65320	29806 ChEBI	fumarate	-	assimilation
65320	16813 ChEBI	galactitol	-	assimilation
65320	28260 ChEBI	galactose	-	assimilation
65320	16865 ChEBI	gamma-aminobutyric acid	-	assimilation
65320	5291 ChEBI	gelatin	-	hydrolysis
65320	17234 ChEBI	glucose	-	assimilation
65320	17754 ChEBI	glycerol	-	assimilation
65320	15428 ChEBI	glycine	-	assimilation
65320	28087 ChEBI	glycogen	-	assimilation
65320	15443 ChEBI	inulin	-	assimilation
65320	16977 ChEBI	L-alanine	-	assimilation
65320	16467 ChEBI	L-arginine	+	assimilation
65320	17196 ChEBI	L-asparagine	-	assimilation
65320	29985 ChEBI	L-glutamate	-	assimilation
65320	15971 ChEBI	L-histidine	-	assimilation
65320	17191 ChEBI	L-isoleucine	-	assimilation
65320	15603 ChEBI	L-leucine	-	assimilation
65320	18019 ChEBI	L-lysine	+	assimilation
65320	16643 ChEBI	L-methionine	-	assimilation
65320	17295 ChEBI	L-phenylalanine	-	assimilation
65320	17203 ChEBI	L-proline	-	assimilation
65320	17115 ChEBI	L-serine	+	assimilation
65320	16857 ChEBI	L-threonine	-	assimilation
65320	16828 ChEBI	L-tryptophan	-	assimilation
65320	17895 ChEBI	L-tyrosine	-	assimilation
65320	16414 ChEBI	L-valine	-	assimilation
65320	24996 ChEBI	lactate	-	assimilation
65320	17716 ChEBI	lactose	-	assimilation
65320	25115 ChEBI	malate	-	assimilation
65320	17306 ChEBI	maltose	-	assimilation
65320	29864 ChEBI	mannitol	-	assimilation
65320	37684 ChEBI	mannose	-	assimilation
65320	6731 ChEBI	melezitose	-	assimilation
65320	28053 ChEBI	melibiose	-	assimilation
65320		milk	-	assimilation
65320	17268 ChEBI	myo-inositol	-	assimilation
65320	17632 ChEBI	nitrate	-	reduction
65320	17309 ChEBI	pectin	-	assimilation
65320	15361 ChEBI	pyruvate	-	assimilation
65320	16634 ChEBI	raffinose	-	assimilation
65320	26546 ChEBI	rhamnose	-	assimilation
65320	33942 ChEBI	ribose	-	assimilation
65320	17814 ChEBI	salicin	-	assimilation
65320	30911 ChEBI	sorbitol	-	assimilation
65320	27922 ChEBI	sorbose	-	assimilation
65320	28017 ChEBI	starch	-	assimilation
65320	28017 ChEBI	starch	-	hydrolysis
65320	30031 ChEBI	succinate	-	assimilation
65320	17992 ChEBI	sucrose	-	assimilation
65320	27082 ChEBI	trehalose	-	assimilation
65320	37166 ChEBI	xylan	-	assimilation
65320	18222 ChEBI	xylose	-	assimilation

Metabolite production

@ref	Chebi-ID	Metabolite	Production
65320	16136 ChEBI	hydrogen sulfide
65320	35581 ChEBI	indole

Enzymes

@ref	Value	Activity	Ec
65320	catalase	-	1.11.1.6
65320	cytochrome oxidase	-	1.9.3.1
65320	lecithinase	-
65320	lipase	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Waste	#Solid animal waste
#Engineered	#Waste	#Solid waste
#Engineered	#Bioreactor	-

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent	Isolation procedure
65052	Methanogenic reactor of cattlefarms	Hokkaido	Japan	JPN	Asia
65320	methanogenic reactor treating waste from cattle farms	Betsukai-machi, Hokkaido	Japan	JPN	Asia	anaerobic roll-tube method
67770	Methanogenic reactor treating waste from cattle farms	Betsukai-machi, Hokkaido	Japan	JPN	Asia

Taxonmaps

69479

Global distribution of 16S sequence AB298771 (>99% sequence identity) for Aminipila butyrica subclade from Microbeatlas

Aquatic Samples	14
Soil Samples	15
Animal Samples	90
Plant Samples	23
Total Samples	142

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
65052	1	Risk group (German classification)

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM1066930v1 assembly for Aminipila butyrica DSM 103574	complete	GCA_010669305	433296.3	2883526296	433296	96.26

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
65052	Aminipila butyrica gene for 16S rRNA, partial sequence	AB298771	1460		433296

GC content

@ref	GC-content (mol%)	Method
65052	44.7
65320	44.5-44.9	high performance liquid chromatography (HPLC)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Aminipila butyrica strain DSM 103574
PATRIC: 433296.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	yes	76.60	no
125439	motility	BacteriaNetⓘ	yes	83.30	no
125439	gram_stain	BacteriaNetⓘ	variable	74.90	no
125439	oxygen_tolerance	BacteriaNetⓘ	anaerobe	99.00	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Aminipila butyrica DSM 103574
NCBI: GCA_010669305.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	53.02	no
125438	anaerobic	anaerobicⓘ	yes	92.07	no
125438	aerobic	aerobicⓘ	no	97.24	no
125438	spore-forming	spore-formingⓘ	yes	59.51	no
125438	thermophilic	thermophileⓘ	no	82.28	yes
125438	flagellated	motile2+ⓘ	yes	74.30	yes

Literature

Topic	Title	Authors	Journal	DOI	Year
	Genomic and physiological properties of Anoxybacterium hadale gen. nov. sp. nov. reveal the important role of dissolved organic sulfur in microbial metabolism in hadal ecosystems.	Cao J, Shao B, Lin J, Liu J, Cui Y, Wang J, Fang J.	Front Microbiol	10.3389/fmicb.2024.1423245	2024
	Hominibacterium faecale gen. nov., sp. nov., an anaerobic L-arginine-degrading bacterium isolated from human feces.	Borhanudin N, Yang M, Chaplin AV, Li J, Wang Q, Dai LR, Wu KJ, Ma SC, Efimov BA, Cheng L.	Arch Microbiol	10.1007/s00203-022-03365-z	2022
Phylogeny	Aminipila luticellarii sp. nov., an anaerobic bacterium isolated from the pit mud of strong aromatic Chinese liquor, and emended description of the genus Aminipila.	Wei Z, Ma S, Chen R, Wu W, Fan H, Dai L, Deng Y	Int J Syst Evol Microbiol	10.1099/ijsem.0.004710	2021
Phylogeny	Aminipila terrae sp. nov., a strictly anaerobic bacterium isolated from river sediment.	Kim YB, Kim JY, Kim J, Song HS, Whon TW, Lee SH, Yoo S, Myoung J, Son HS, Roh SW	Arch Microbiol	10.1007/s00203-021-02301-x	2021
Phylogeny	Aminipila butyrica gen. nov., sp. nov., a strictly anaerobic, arginine-decomposing bacterium isolated from a methanogenic reactor of cattle waste.	Ueki A, Goto K, Kaku N, Ueki K	Int J Syst Evol Microbiol	10.1099/ijsem.0.002534	2017

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#65052	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 103574
#65320	Atsuko Ueki, Kazushi Goto, Nobuo Kaku, Katsuji Ueki: Aminipila butyrica gen. nov., sp. nov., a strictly anaerobic, arginine-decomposing bacterium isolated from a methanogenic reactor of cattle waste. IJSEM 68: 443 - 448 2018 ( DOI 10.1099/ijsem.0.002534 , PubMed 29235979 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Aminipila butyrica (DSM 103574, JCM 31555, FH042)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Observation

Metabolite utilization

Metabolite production

Enzymes

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_010669305

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

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