Name: Aminipila butyrica FH042
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 158376

Type strain:

Species: Aminipila butyrica

Strain Designation: FH042

Culture col. no.: DSM 103574, JCM 31555

Strain history: A. Ueki; Fac. of Agric., Yamagata Univ., Japan; FH042.

NCBI tax ID(s): 433296 (species)

SI-ID 396548

DSM 103574

Section

Aminipila butyrica FH042 is an anaerobe, mesophilic, Gram-positive bacterium that was isolated from Methanogenic reactor of cattlefarms.

Gram-positive
motile
rod-shaped
anaerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Clostridia*
	Order	*Eubacteriales*
	Family	"Anaerovoracaceae"
	Genus	*Aminipila*
	Species	**Aminipila butyrica**
	Full Scientific Name (LPSN)	Aminipila butyrica Ueki et al. 2018

Information on morphological and physiological properties Morphology

[Ref.: #65320]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #65320]	Cell length	3-8 µm

[Ref.: #65320]	Cell width	0.7-1 µm

[Ref.: #65320]	Cell shape	rod-shaped

[Ref.: #65320]	Motility	yes

[Ref.: #65320]	Flagellum arrangement	polar

Information on culture and growth conditions Culture and growth conditions

[Ref.: #65052]

Culture medium

PY + X MEDIUM (N2/CO2) (DSMZ Medium 104c)

[Ref.: #65052]

Culture medium growth

[Ref.: #65052]

Culture medium link

Medium recipe at MediaDive

[Ref.: #65052]

Culture medium composition

expand / minimize

[Ref.: #65320]

Culture medium

PY4S

[Ref.: #65320]

Culture medium growth

[Ref.: #65320]

Culture medium

chopped meat broth

[Ref.: #65320]

Culture medium growth

		Temperature
[Ref.: #65052]	growth	30 °C
[Ref.: #65320]	growth	10-35 °C
[Ref.: #65320]	optimum	30 °C
[Ref.: #67770]	growth	30 °C

[Ref.: #65052]	Temperature range	mesophilic
[Ref.: #65320]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #65320]			optimum	6.1-6.8
[Ref.: #65320]			growth	5.3-8.2

Information on physiology and metabolism Physiology and metabolism

[Ref.: #69480]

Oxygen tolerance

anaerobe Confidence in %99.999

[Ref.: #65320]

Ability of spore formation

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #65320]		NaCl		growth	0-20	g/L
[Ref.: #65320]		NaCl		optimum	0	g/L

[Ref.: #65320]	Observation	Longer rods up to 40 µm often occur.
[Ref.: #65320]	Observation	B-vitamins are not required for growth.

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #65320]	amygdalin ChEBI	-	assimilation
[Ref.: #65320]	arabinose ChEBI	-	assimilation
[Ref.: #65320]	carboxymethylcellulose ChEBI	-	assimilation
[Ref.: #65320]	casamino acids	+	assimilation
[Ref.: #65320]	cellobiose ChEBI	-	assimilation
[Ref.: #65320]	cellulose ChEBI	-	assimilation
[Ref.: #65320]	dextrin ChEBI	-	assimilation
[Ref.: #65320]	esculin ChEBI	-	assimilation
[Ref.: #65320]	esculin ChEBI	-	hydrolysis
[Ref.: #65320]	fructose ChEBI	-	assimilation
[Ref.: #65320]	fumarate ChEBI	-	assimilation
[Ref.: #65320]	galactitol ChEBI	-	assimilation
[Ref.: #65320]	galactose ChEBI	-	assimilation
[Ref.: #65320]	gamma-aminobutyric acid ChEBI	-	assimilation
[Ref.: #65320]	gelatin ChEBI	-	hydrolysis
[Ref.: #65320]	glucose ChEBI	-	assimilation
[Ref.: #65320]	glycerol ChEBI	-	assimilation
[Ref.: #65320]	glycine ChEBI	-	assimilation
[Ref.: #65320]	glycogen ChEBI	-	assimilation
[Ref.: #65320]	inulin ChEBI	-	assimilation
[Ref.: #65320]	L-alanine ChEBI	-	assimilation
[Ref.: #65320]	L-arginine ChEBI	+	assimilation
[Ref.: #65320]	L-asparagine ChEBI	-	assimilation
[Ref.: #65320]	L-glutamate ChEBI	-	assimilation
[Ref.: #65320]	L-histidine ChEBI	-	assimilation
[Ref.: #65320]	L-isoleucine ChEBI	-	assimilation
[Ref.: #65320]	L-leucine ChEBI	-	assimilation
[Ref.: #65320]	L-lysine ChEBI	+	assimilation
[Ref.: #65320]	L-methionine ChEBI	-	assimilation
[Ref.: #65320]	L-phenylalanine ChEBI	-	assimilation
[Ref.: #65320]	L-proline ChEBI	-	assimilation
[Ref.: #65320]	L-serine ChEBI	+	assimilation
[Ref.: #65320]	L-threonine ChEBI	-	assimilation
[Ref.: #65320]	L-tryptophan ChEBI	-	assimilation
[Ref.: #65320]	L-tyrosine ChEBI	-	assimilation
[Ref.: #65320]	L-valine ChEBI	-	assimilation
[Ref.: #65320]	lactate ChEBI	-	assimilation
[Ref.: #65320]	lactose ChEBI	-	assimilation
[Ref.: #65320]	malate ChEBI	-	assimilation
[Ref.: #65320]	maltose ChEBI	-	assimilation
[Ref.: #65320]	mannitol ChEBI	-	assimilation
[Ref.: #65320]	mannose ChEBI	-	assimilation
[Ref.: #65320]	melezitose ChEBI	-	assimilation
[Ref.: #65320]	melibiose ChEBI	-	assimilation
[Ref.: #65320]	milk	-	assimilation
[Ref.: #65320]	myo-inositol ChEBI	-	assimilation
[Ref.: #65320]	nitrate ChEBI	-	reduction
[Ref.: #65320]	pectin ChEBI	-	assimilation
[Ref.: #65320]	pyruvate ChEBI	-	assimilation
[Ref.: #65320]	raffinose ChEBI	-	assimilation
[Ref.: #65320]	rhamnose ChEBI	-	assimilation
[Ref.: #65320]	ribose ChEBI	-	assimilation
[Ref.: #65320]	salicin ChEBI	-	assimilation
[Ref.: #65320]	sorbitol ChEBI	-	assimilation
[Ref.: #65320]	sorbose ChEBI	-	assimilation
[Ref.: #65320]	starch ChEBI	-	assimilation
[Ref.: #65320]	starch ChEBI	-	hydrolysis
[Ref.: #65320]	succinate ChEBI	-	assimilation
[Ref.: #65320]	sucrose ChEBI	-	assimilation
[Ref.: #65320]	trehalose ChEBI	-	assimilation
[Ref.: #65320]	xylan ChEBI	-	assimilation
[Ref.: #65320]	xylose ChEBI	-	assimilation
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #65320]		hydrogen sulfide ChEBI	yes
[Ref.: #65320]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #65320]	catalase	-	1.11.1.6
[Ref.: #65320]	cytochrome oxidase	-	1.9.3.1
[Ref.: #65320]	lecithinase	-
[Ref.: #65320]	lipase	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #65052]	Sample type/isolated from	Methanogenic reactor of cattlefarms
[Ref.: #65052]	Geographic location (country and/or sea, region)	Hokkaido
[Ref.: #65052]	Country	Japan
[Ref.: #65052]	Country ISO 3 Code	JPN
[Ref.: #65052]	Continent	Asia

[Ref.: #65320]	Sample type/isolated from	methanogenic reactor treating waste from cattle farms
[Ref.: #65320]	Geographic location (country and/or sea, region)	Betsukai-machi, Hokkaido
[Ref.: #65320]	Country	Japan
[Ref.: #65320]	Country ISO 3 Code	JPN
[Ref.: #65320]	Continent	Asia
[Ref.: #65320]	Isolation/enrichment procedure	anaerobic roll-tube method

[Ref.: #67770]	Sample type/isolated from	Methanogenic reactor treating waste from cattle farms
[Ref.: #67770]	Geographic location (country and/or sea, region)	Betsukai-machi, Hokkaido
[Ref.: #67770]	Country	Japan
[Ref.: #67770]	Country ISO 3 Code	JPN
[Ref.: #67770]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Engineered	#Waste	#Solid animal waste
#Engineered	#Waste	#Solid waste
#Engineered	#Bioreactor	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AB298771 (>99% sequence identity) for Aminipila butyrica subclade from Microbeatlas

Aquatic Samples	14
Soil Samples	15
Animal Samples	90
Plant Samples	23
Total Samples	142

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #65052]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #65052]	Aminipila butyrica gene for 16S rRNA, partial sequence	AB298771	1460	ENA	433296 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Aminipila butyrica DSM 103574	GCA_010669305	complete	GenBank	433296 tax ID	*
[Ref.: #66792]	Aminipila butyrica strain DSM 103574	433296.3	complete	PATRIC	433296 tax ID	*
[Ref.: #66792]	Aminipila butyrica DSM 103574	2883526296	complete	JGI IMG/M	433296 tax ID	*

[Ref.: #65052]	GC-content	44.7 mol%
[Ref.: #65320]	GC-content	44.5-44.9 mol%	high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Aminipila butyrica DSM 103574 NCBI: GCA_010669305.1
[Ref.: #69481]	spore-forming	no	67	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Aminipila butyrica DSM 103574 NCBI: GCA_010669305.1
[Ref.: #69480]	motile	yes	89.24	yes
[Ref.: #69480]	flagellated	yes	54.131	yes
[Ref.: #69480]	gram-positive	no	66.749	no
[Ref.: #69480]	anaerobic	yes	99.44	no
[Ref.: #69480]	aerobic	no	97.819	no
[Ref.: #69480]	halophile	no	96.173	yes
[Ref.: #69480]	spore-forming	yes	80.29	yes
[Ref.: #69480]	thermophile	no	99.052	yes
[Ref.: #69480]	glucose-util	yes	56.457	yes
[Ref.: #69480]	glucose-ferment	yes	54.463	no

Availability in culture collections External links

[Ref.: #65052]

Culture collection no.

DSM 103574, JCM 31555

[Ref.: #111181]

SI-ID 396548

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Aminipila luticellarii sp. nov., an anaerobic bacterium isolated from the pit mud of strong aromatic Chinese liquor, and emended description of the genus Aminipila.	Wei Z, Ma S, Chen R, Wu W, Fan H, Dai L, Deng Y	Int J Syst Evol Microbiol	10.1099/ijsem.0.004710	2021	*
Phylogeny	Aminipila terrae sp. nov., a strictly anaerobic bacterium isolated from river sediment.	Kim YB, Kim JY, Kim J, Song HS, Whon TW, Lee SH, Yoo S, Myoung J, Son HS, Roh SW	Arch Microbiol	10.1007/s00203-021-02301-x	2021	*
Phylogeny	Aminipila butyrica gen. nov., sp. nov., a strictly anaerobic, arginine-decomposing bacterium isolated from a methanogenic reactor of cattle waste.	Ueki A, Goto K, Kaku N, Ueki K	Int J Syst Evol Microbiol	10.1099/ijsem.0.002534	2017	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#65052

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 103574

#65320

Atsuko Ueki, Kazushi Goto, Nobuo Kaku, Katsuji Ueki: Aminipila butyrica gen. nov., sp. nov., a strictly anaerobic, arginine-decomposing bacterium isolated from a methanogenic reactor of cattle waste. IJSEM 68: 443 - 448 2018 ( DOI 10.1099/ijsem.0.002534 , PubMed 29235979 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#111181

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID396548.1 )

* These data were automatically processed and therefore are not curated

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Aminipila butyrica DSM 103574

Aminipila butyrica DSM 103574

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