Name: Gellertiella hungarica RAM11
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 158276

Type strain:

Species: Gellertiella hungarica

Strain Designation: RAM11

Culture col. no.: DSM 29853, NCAIM B.02618

Strain history: <- E. Toth, Eötvös Loránd Univ. Budapest, Hungary; RAM11 <- S. Szuróczki and Z. Kéki

NCBI tax ID(s): 1572859 (species)

SI-ID 404510

DSM 29853

Section

Gellertiella hungarica RAM11 is an obligate aerobe, mesophilic, Gram-negative bacterium that forms circular colonies and was isolated from water of a thermal bath.

colony-forming
Gram-negative
motile
rod-shaped
mesophilic
obligate aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Alphaproteobacteria*
	Order	*Hyphomicrobiales*
	Family	*Rhizobiaceae*
	Genus	*Gellertiella*
	Species	**Gellertiella hungarica**
	Full Scientific Name (LPSN)	Gellertiella hungarica Tóth et al. 2017

Information on morphological and physiological properties Morphology

[Ref.: #65496]	Gram stain	negative

[Ref.: #65496]	Cell length	1.1-2.1 µm

[Ref.: #65496]	Cell width	0.4-0.6 µm

[Ref.: #65496]	Cell shape	rod-shaped

[Ref.: #65496]	Motility	yes

[Ref.: #65496]	Flagellum arrangement	polar

[Ref.: #65496]	Colony size	1-2 mm
[Ref.: #65496]	Colony color	white
[Ref.: #65496]	Colony shape	circular
[Ref.: #65496]	Cultivation medium used	R2A

Information on culture and growth conditions Culture and growth conditions

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Culture medium

NUTRIENT AGAR (DSMZ Medium 1)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

expand / minimize

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Culture medium

YMA

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Culture medium growth

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Culture medium

Reasoner's 2A agar (R2A)

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Culture medium growth

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Culture medium

Trypticase Soy Agar (TSA)

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Culture medium growth

		Temperature
[Ref.: #64936]	growth	28 °C
[Ref.: #65496]	growth	15-45 °C
[Ref.: #65496]	optimum	20-37 °C

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Temperature range

mesophilic

	pH	Kind of pH		pH
[Ref.: #65496]			optimum	7-9
[Ref.: #65496]			growth	5-10

Information on physiology and metabolism Physiology and metabolism

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Oxygen tolerance

obligate aerobe

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Halophily / tolerance level

non-halophilic

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #65496]		NaCl		growth	0-1	%(w/v)
[Ref.: #65496]		NaCl		optimum	0	%(w/v)

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Observation

budding

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #65496]	amygdalin ChEBI	-	builds acid from
[Ref.: #65496]	arbutin ChEBI	-	builds acid from
[Ref.: #65496]	cellobiose ChEBI	-	builds acid from
[Ref.: #65496]	D-arabinose ChEBI	-	builds acid from
[Ref.: #65496]	D-arabitol ChEBI	-	builds acid from
[Ref.: #65496]	D-fructose ChEBI	+	builds acid from
[Ref.: #65496]	D-fucose ChEBI	-	builds acid from
[Ref.: #65496]	D-galactose ChEBI	-	builds acid from
[Ref.: #65496]	D-glucose ChEBI	-	builds acid from
[Ref.: #65496]	D-lyxose ChEBI	-	builds acid from
[Ref.: #65496]	D-mannitol ChEBI	-	builds acid from
[Ref.: #65496]	D-mannose ChEBI	-	builds acid from
[Ref.: #65496]	D-ribose ChEBI	-	builds acid from
[Ref.: #65496]	D-sorbitol ChEBI	-	builds acid from
[Ref.: #65496]	D-tagatose ChEBI	-	builds acid from
[Ref.: #65496]	D-xylose ChEBI	-	builds acid from
[Ref.: #65496]	erythritol ChEBI	-	builds acid from
[Ref.: #65496]	esculin ChEBI	-	hydrolysis
[Ref.: #65496]	esculin ferric citrate	-	builds acid from
[Ref.: #65496]	galactitol ChEBI	-	builds acid from
[Ref.: #65496]	gentiobiose ChEBI	-	builds acid from
[Ref.: #65496]	glycerol ChEBI	-	builds acid from
[Ref.: #65496]	glycogen ChEBI	-	builds acid from
[Ref.: #65496]	inulin ChEBI	-	builds acid from
[Ref.: #65496]	L-arabinose ChEBI	-	builds acid from
[Ref.: #65496]	L-arabitol ChEBI	-	builds acid from
[Ref.: #65496]	L-fucose ChEBI	-	builds acid from
[Ref.: #65496]	L-rhamnose ChEBI	-	builds acid from
[Ref.: #65496]	L-sorbose ChEBI	-	builds acid from
[Ref.: #65496]	L-xylose ChEBI	-	builds acid from
[Ref.: #65496]	lactose ChEBI	-	builds acid from
[Ref.: #65496]	maltose ChEBI	-	builds acid from
[Ref.: #65496]	melezitose ChEBI	-	builds acid from
[Ref.: #65496]	melibiose ChEBI	-	builds acid from
[Ref.: #65496]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from
[Ref.: #65496]	methyl alpha-D-mannoside ChEBI	-	builds acid from
[Ref.: #65496]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from
[Ref.: #65496]	myo-inositol ChEBI	-	builds acid from
[Ref.: #65496]	N-acetylglucosamine ChEBI	-	builds acid from
[Ref.: #65496]	nitrate ChEBI	-	reduction
[Ref.: #65496]	potassium 2-dehydro-D-gluconate	-	builds acid from
[Ref.: #65496]	potassium 5-dehydro-D-gluconate	-	builds acid from
[Ref.: #65496]	potassium gluconate ChEBI	-	builds acid from
[Ref.: #65496]	raffinose ChEBI	-	builds acid from
[Ref.: #65496]	ribitol ChEBI	-	builds acid from
[Ref.: #65496]	salicin ChEBI	-	builds acid from
[Ref.: #65496]	starch ChEBI	-	builds acid from
[Ref.: #65496]	sucrose ChEBI	-	builds acid from
[Ref.: #65496]	trehalose ChEBI	-	builds acid from
[Ref.: #65496]	turanose ChEBI	-	builds acid from
[Ref.: #65496]	xylitol ChEBI	-	builds acid from
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Concentration		Antibiotic resistance	Concentration
[Ref.: #65496]	carbenicillin ChEBI	yes	100	µg (disc)
[Ref.: #65496]	cefamandole ChEBI				yes	30	µg (disc)
[Ref.: #65496]	cefoxitin ChEBI	yes	30	µg (disc)
[Ref.: #65496]	cefuroxime ChEBI	yes	30	µg (disc)
[Ref.: #65496]	ciprofloxacin ChEBI	yes	5	µg (disc)
[Ref.: #65496]	clindamycin ChEBI				yes	2	µg (disc)
[Ref.: #65496]	erythromycin ChEBI	yes	15	µg (disc)
[Ref.: #65496]	gentamicin ChEBI	yes	120	µg (disc)
[Ref.: #65496]	imipenem ChEBI	yes	10	µg (disc)
[Ref.: #65496]	linezolid ChEBI	yes	30	µg (disc)
[Ref.: #65496]	meropenem ChEBI	yes	10	µg (disc)
[Ref.: #65496]	neomycin ChEBI	yes	30	Unit (disc)
[Ref.: #65496]	netilmycin ChEBI	yes	30	µg (disc)
[Ref.: #65496]	penicillin g ChEBI	yes	6	µg (disc)
[Ref.: #65496]	polymyxin b ChEBI				yes	300	Unit (disc)
[Ref.: #65496]	tobramycin ChEBI	yes	10	µg (disc)
[Ref.: #65496]	vancomycin ChEBI	yes	30	µg (disc)
	Only first 10 entries are displayed. Click here to see all.

	Group ID of combined antibiotics	Metabolite	Antibiotic sensitivity	Concentration
[Ref.: #65496]	11	amoxicillin ChEBI	yes	20	µg (disc)
[Ref.: #65496]	11	clavulanic acid ChEBI	yes	10	µg (disc)
[Ref.: #65496]	16	piperacillin ChEBI	yes	100	µg (disc)
[Ref.: #65496]	16	tazobactam ChEBI	yes	10	µg (disc)

	Enzyme	Enzyme activity	EC number
[Ref.: #65496]	acid phosphatase	+	3.1.3.2
[Ref.: #65496]	alkaline phosphatase	-	3.1.3.1
[Ref.: #65496]	alpha-Amylase	+	3.2.1.1
[Ref.: #65496]	alpha-fucosidase	-	3.2.1.51
[Ref.: #65496]	alpha-galactosidase	-	3.2.1.22
[Ref.: #65496]	alpha-glucosidase	+	3.2.1.20
[Ref.: #65496]	alpha-mannosidase	-	3.2.1.24
[Ref.: #65496]	beta-galactosidase	-	3.2.1.23
[Ref.: #65496]	beta-glucosidase	-	3.2.1.21
[Ref.: #65496]	beta-glucuronidase	-	3.2.1.31
[Ref.: #65496]	caseinase	+	3.4.21.50
[Ref.: #65496]	catalase	+	1.11.1.6
[Ref.: #65496]	cytochrome oxidase	+	1.9.3.1
[Ref.: #65496]	esterase (C 4)	+
[Ref.: #65496]	esterase Lipase (C 8)	+
[Ref.: #65496]	gelatinase	+
[Ref.: #65496]	leucine arylamidase	+	3.4.11.1
[Ref.: #65496]	lipase (C 14)	-
[Ref.: #65496]	N-acetyl-beta-glucosaminidase	+	3.2.1.52
[Ref.: #65496]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #65496]	phosphatase	+
[Ref.: #65496]	trypsin	+	3.4.21.4
[Ref.: #65496]	urease	-	3.5.1.5
[Ref.: #65496]	valine arylamidase	-
	Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #64936]	Sample type/isolated from	water of a thermal bath
[Ref.: #64936]	Geographic location (country and/or sea, region)	Budapest
[Ref.: #64936]	Country	Hungary
[Ref.: #64936]	Country ISO 3 Code	HUN
[Ref.: #64936]	Continent	Europe

[Ref.: #65496]	Sample type/isolated from	water draining from a well into the pool of a thermal bath
[Ref.: #65496]	Sampling date	2013-10-28
[Ref.: #65496]	Geographic location (country and/or sea, region)	Gellért Thermanl Bath, Budapest
[Ref.: #65496]	Country	Hungary
[Ref.: #65496]	Country ISO 3 Code	HUN
[Ref.: #65496]	Continent	Europe
[Ref.: #65496]	Enrichment culture	R2A
[Ref.: #65496]	Enrichment culture duration	3 weeks
[Ref.: #65496]	Enrichment culture temperature	37 ̊C
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Thermal spring
#Engineered	#Built environment	#Water reservoir (Aquarium/pool)

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

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Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #64936]	Sinorhizobium sp. RAM11 partial 16S rRNA gene, strain RAM11, isolate 60	LN651200	1493	ENA	1572859 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Gellertiella hungarica DSM 29853	GCA_014197145	scaffold	GenBank	1572859 tax ID	*
[Ref.: #66792]	Gellertiella hungarica strain DSM 29853	1572859.3	wgs	PATRIC	1572859 tax ID	*
[Ref.: #66792]	Gellertiella hungarica DSM 29853	2830095549	draft	JGI IMG/M	1572859 tax ID	*

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GC-content

62.9 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Gellertiella hungarica DSM 29853 NCBI: GCA_014197145.1
[Ref.: #69480]	flagellated	no	56.14	yes
[Ref.: #69480]	gram-positive	no	98.281	no
[Ref.: #69480]	anaerobic	no	97.863	yes
[Ref.: #69480]	aerobic	yes	88.989	yes
[Ref.: #69480]	halophile	no	94.34	yes
[Ref.: #69480]	spore-forming	no	95.935	no
[Ref.: #69480]	glucose-util	yes	89.66	no
[Ref.: #69480]	thermophile	no	97.437	yes
[Ref.: #69480]	motile	yes	87.198	no
[Ref.: #69480]	glucose-ferment	no	92.281	no

Availability in culture collections External links

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Culture collection no.

DSM 29853, NCAIM B.02618

[Ref.: #111094]

SI-ID 404510

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Gellertiella hungarica gen. nov., sp. nov., a novel bacterium of the family Rhizobiaceae isolated from a spa in Budapest.	Toth E, Szuroczki S, Keki Z, Boka K, Szili-Kovacs T, Schumann P	Int J Syst Evol Microbiol	10.1099/ijsem.0.002332	2017	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#64936

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 29853

#65496

Erika Tóth, Sára Szuróczki, Zsuzsa Kéki, Károly Bóka, Tibor Szili-Kovács and Peter Schumann: Gellertiella hungarica gen. nov., sp. nov., a novel bacterium of the family Rhizobiaceae isolated from a spa in Budapest. IJSEM 67: 4565 - 4571 2017 ( DOI 10.1099/ijsem.0.002332 , PubMed 28945538 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#111094

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID404510.1 )

* These data were automatically processed and therefore are not curated

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Gellertiella hungarica DSM 29853

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