[Ref.: #64906] |
Sample type/isolated from |
soil (silt) from the bottom of a river |
[Ref.: #64906] |
Geographic location (country and/or sea, region) |
Lac region (13° 38' N, 15° 22' E) |
[Ref.: #64906] |
Country |
Chad |
[Ref.: #64906] |
Country ISO 3 Code |
TCD |
[Ref.: #64906] |
Continent |
Africa |
[Ref.: #64906] |
Geographic location |
13.6333°/15.3667° |
|
[Ref.: #65399] |
Sample type/isolated from |
soil sample collected from the bottom of a river |
[Ref.: #65399] |
Geographic location (country and/or sea, region) |
Chad |
[Ref.: #65399] |
Enrichment culture |
sodium succinate-asparagine agar medium |
[Ref.: #65399] |
Enrichment culture composition |
(0.2 g asparagine; 1 g sodium succinate; 0.2 g CaCl2 x 2H2O; 1 mg FeSO4 x 7H2O; 0.3 g KCl; 0.9 g KH2PO4; 0.6 g K2HPO4 x 3H2O; 20 g agar; 1 l distilled water; pH, 7.2) supplemented with cycloheximide (50 mg l-1) and nalidixic acid (20 mg l-1). |
[Ref.: #65399] |
Enrichment culture duration |
20 days |
[Ref.: #65399] |
Enrichment culture temperature |
28 ̊C |
[Ref.: #65399] |
Isolation/enrichment procedure |
The soil sample (5 g) was suspended in distilled water (2 ml) followed by ultra sonic treatment (160 W) for 3 min. After the addition of distilled water (43 ml), the soil suspension was incubated at 28°C and 250 r.p.m. on a rotary shaker for 30 min. Subsequently, a 200 µl sample of the suspension was spread on a plate of sodium succinate-asparagine agar medium. 20 days of aerobic incubation at 28°C |
|
[Ref.: #67770] |
Sample type/isolated from |
Soil from the bottom of a river,(13° 38' N, 15° 22' E) |
[Ref.: #67770] |
Country |
Chad |
[Ref.: #67770] |
Country ISO 3 Code |
TCD |
[Ref.: #67770] |
Continent |
Africa |
* marker position based on {}
|
|
Isolation sources categories |
#Environmental |
#Aquatic |
#River (Creek) |
#Environmental |
#Terrestrial |
#Soil |
|
|
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Availability in culture collections External links
[Ref.: #64906] |
Culture collection no. |
DSM 105123, CGMCC 4.7397, JCM 32387 |
[Ref.: #111072] |
SI-ID 407439
|
* |
|
Literature: |
|
Topic |
Title |
Authors |
Journal |
DOI |
Year |
|
Phylogeny |
Pseudonocardia lutea sp. nov., a novel actinobacterium isolated from soil in Chad. |
Gao Y, Piao C, Wang H, Shi L, Guo X, Song J, Xiang W, Zhao J, Wang X |
Int J Syst Evol Microbiol |
10.1099/ijsem.0.002780 |
2018 |
* |
|
- References
-
-
#64906 |
Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ;
DSM 105123
|
-
#65399 |
Yuhang Gao, Chenyu Piao, Han Wang, Linlin Shi, Xiaowei Guo, Jia Song, Wensheng Xiang, Junwei Zhao, Xiangjing Wang:
Pseudonocardia lutea sp. nov., a novel actinobacterium isolated from soil in Chad.
IJSEM 2018:
1992 - 1997
68 (
DOI 10.1099/ijsem.0.002780 , PubMed 29683415 )
|
-
#67770 |
Japan Collection of Microorganism (JCM) ; Curators of the JCM;
|
-
#111072 |
Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.:
StrainInfo: A central database for resolving microbial strain identifiers
.
(
DOI 10.60712/SI-ID407439.1 )
|
- * These data were automatically processed and therefore are not curated
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