Name: Pseudobacteroides cellulosolvens WM 2
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 1581

Type strain:

Species: Pseudobacteroides cellulosolvens

Strain Designation: WM 2

Culture col. no.: DSM 2933, ATCC 35603, NRCC 2944

Strain history: <- W.D. Murray, WM 2

NCBI tax ID(s): 35825 (species)

SI-ID 41189

ATCC 35603, DSM 2933, CCUG 52234

Section

Pseudobacteroides cellulosolvens WM 2 is an anaerobe, spore-forming, mesophilic bacterium that was isolated from municipal sewage sludge.

motile
spore-forming
anaerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Clostridia*
	Order	*Eubacteriales*
	Family	*Oscillospiraceae*
	Genus	*Pseudobacteroides*
	Species	**Pseudobacteroides cellulosolvens**
	Full Scientific Name (LPSN)	Pseudobacteroides cellulosolvens (Murray et al. 1984) Horino et al. 2014

Synonym

Bacteroides cellulosolvens

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	positive Confidence in %99.999

[Ref.: #69480]	Motility	yes Confidence in %92.413

[Ref.: #1253]

Incubation period

3-7 days

Information on culture and growth conditions Culture and growth conditions

[Ref.: #1253]

Culture medium

BACTEROIDES CELLULOSOLVENS MEDIUM (DSMZ Medium 315)

[Ref.: #1253]

Culture medium growth

[Ref.: #1253]

Culture medium link

Medium recipe at MediaDive

[Ref.: #1253]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #1253]			growth	35 °C

[Ref.: #1253]

Temperature range

mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #1253]	Oxygen tolerance	anaerobe
[Ref.: #69480]	Oxygen tolerance	anaerobe Confidence in %99.621

[Ref.: #69480]

Ability of spore formation

yes Confidence in %100

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68380]	arginine ChEBI	-	hydrolysis	* from API rID32A
[Ref.: #68380]	D-mannose ChEBI	-	fermentation	* from API rID32A
[Ref.: #68380]	L-glutamate ChEBI	-	degradation	* from API rID32A
[Ref.: #68380]	nitrate ChEBI	-	reduction	* from API rID32A
[Ref.: #68380]	raffinose ChEBI	-	fermentation	* from API rID32A
[Ref.: #68380]	tryptophan ChEBI	-	energy source	* from API rID32A
[Ref.: #68380]	urea ChEBI	-	hydrolysis	* from API rID32A

	Metabolite production	Metabolite	Production
[Ref.: #68380]		indole ChEBI	no	* from API rID32A

	Physiological tests	Metabolite	Indole test
[Ref.: #68380]		indole ChEBI	-	* from API rID32A

	Enzyme	Enzyme activity	EC number
[Ref.: #68380]	alanine arylamidase	-	3.4.11.2	* from API rID32A
[Ref.: #68380]	alkaline phosphatase	-	3.1.3.1	* from API rID32A
[Ref.: #68380]	alpha-fucosidase	-	3.2.1.51	* from API rID32A
[Ref.: #68380]	alpha-galactosidase	-	3.2.1.22	* from API rID32A
[Ref.: #68380]	alpha-glucosidase	-	3.2.1.20	* from API rID32A
[Ref.: #68380]	arginine dihydrolase	-	3.5.3.6	* from API rID32A
[Ref.: #68380]	beta-galactosidase	-	3.2.1.23	* from API rID32A
[Ref.: #68380]	beta-Galactosidase 6-phosphate	-		* from API rID32A
[Ref.: #68380]	beta-glucosidase	+	3.2.1.21	* from API rID32A
[Ref.: #68380]	beta-glucuronidase	-	3.2.1.31	* from API rID32A
[Ref.: #68380]	glutamate decarboxylase	-	4.1.1.15	* from API rID32A
[Ref.: #68380]	glutamyl-glutamate arylamidase	-		* from API rID32A
[Ref.: #68380]	glycin arylamidase	-		* from API rID32A
[Ref.: #68380]	histidine arylamidase	-		* from API rID32A
[Ref.: #68380]	L-arginine arylamidase	-		* from API rID32A
[Ref.: #68380]	leucine arylamidase	-	3.4.11.1	* from API rID32A
[Ref.: #68380]	leucyl glycin arylamidase	-	3.4.11.1	* from API rID32A
[Ref.: #68380]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API rID32A
[Ref.: #68380]	phenylalanine arylamidase	-		* from API rID32A
[Ref.: #68380]	proline-arylamidase	-	3.4.11.5	* from API rID32A
[Ref.: #68380]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API rID32A
[Ref.: #68380]	serine arylamidase	-		* from API rID32A
[Ref.: #68380]	tryptophan deaminase	-	4.1.99.1	* from API rID32A
[Ref.: #68380]	tyrosine arylamidase	-		* from API rID32A
[Ref.: #68380]	urease	-	3.5.1.5	* from API rID32A
	Only first 10 entries are displayed. Click here to see all.

API® rID32A:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Urease/urea hydrolysisURE	Arginine dihydrolaseADH (Arg)	alpha-Galactosidasealpha GAL	beta-Galactosidasebeta GAL	beta-Galactosidase 6-phosphatebeta GP	alpha-Glucosidasealpha GLU	beta-Glucosidasebeta GLU	alpha-Arabinosidasealpha ARA	beta-Glucuronidasebeta GUR	beta-N-Acetyl-beta-glucosaminidasebeta NAG	Fermentation of D-mannoseMNE	Fermentation of raffinoseRAF	Glutamate decarboxylaseGDC	alpha-Fucosidasealpha FUC	Reduction of nitrateNIT	Indole productionIND	Alkaline phosphatasePAL	L-arginine arylamidaseArgA	Proline arylamidaseProA	Leucyl glycin arylamidaseLGA	Phenylalanine arylamidasePheA	Leucine arylamidaseLeuA	Pyrrolidonyl arylamidasePyrA	Tyrosine arylamidaseTyrA	Alanine arylamidaseAlaA	Glycin arylamidaseGlyA	Histidine arylamidaseHisA	Glutamyl-glutamate arylamidaseGGA	Serine arylamidaseSerA
[Ref.: #1253]	12139	-	-	-	-	-	-	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
[Ref.: #1253]	12138	-	-	-	-	-	-	+	+/-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #1253]	Sample type/isolated from	municipal sewage sludge
[Ref.: #1253]	Country	Canada
[Ref.: #1253]	Country ISO 3 Code	CAN
[Ref.: #1253]	Continent	North America
* marker position based on {}

Isolation sources categories

#Engineered

#Waste

#Sewage sludge

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #1253]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #1253]	Bacteroides cellulosolvens (ATCC 35603) 16S ribosomal RNA (16S rRNA)	L35517	1464	ENA	35825 tax ID
[Ref.: #1253]	Pseudobacteroides cellulosolvens ATCC 35603 = DSM 2933 16S ribosomal RNA, partial sequence	NR_025918	1464	GenBank	398512 tax ID
[Ref.: #20218]	Clostridium sp. K12-15-18 partial 16S rRNA gene, clone K12-15-18	HE862233	1507	ENA	1200986 tax ID	*
[Ref.: #20218]	Clostridium sp. K13-19 partial 16S rRNA gene, clone K13-19	HE862234	1506	ENA	1200987 tax ID	*

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Pseudobacteroides cellulosolvens ATCC 35603 = DSM 2933	GCA_001262605	contig	GenBank	398512 tax ID	*
[Ref.: #66792]	Pseudobacteroides cellulosolvens ATCC 35603 = DSM 2933	GCA_000745845	scaffold	GenBank	398512 tax ID	*
[Ref.: #66792]	Pseudobacteroides cellulosolvens ATCC 35603 = DSM 2933	398512.8	wgs	PATRIC	398512 tax ID	*
[Ref.: #66792]	Pseudobacteroides cellulosolvens ATCC 35603 = DSM 2933	398512.9	wgs	PATRIC	398512 tax ID	*
[Ref.: #66792]	Pseudobacteroides cellulosolvens DSM 2933	2695420353	draft	JGI IMG/M	398512 tax ID	*
[Ref.: #66792]	Pseudobacteroides cellulosolvens DSM 2933	2571042001	draft	JGI IMG/M	398512 tax ID	*

[Ref.: #1253]

GC-content

43.0 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Pseudobacteroides cellulosolvens ATCC 35603 = DSM 2933 PATRIC: 398512.9
[Ref.: #69481]	spore-forming	yes	51	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Pseudobacteroides cellulosolvens ATCC 35603 = DSM 2933 DSM 2933 NCBI: GCA_000745845.1
[Ref.: #69480]	motile	yes	75.519	no
[Ref.: #69480]	flagellated	no	57.884	no
[Ref.: #69480]	gram-positive	no	69.762	no
[Ref.: #69480]	anaerobic	yes	98.581	no
[Ref.: #69480]	aerobic	no	95.441	no
[Ref.: #69480]	halophile	no	97.674	no
[Ref.: #69480]	spore-forming	yes	97.035	no
[Ref.: #69480]	thermophile	no	94.021	yes
[Ref.: #69480]	glucose-util	yes	82.148	no
[Ref.: #69480]	glucose-ferment	no	58.342	no

Availability in culture collections External links

[Ref.: #1253]

Culture collection no.

DSM 2933, ATCC 35603, NRCC 2944

[Ref.: #71226]

SI-ID 41189

Literature:

Topic	Title	Authors	Journal	DOI	Year
Genetics	Near-Complete Genome Sequence of the Cellulolytic Bacterium Bacteroides (Pseudobacteroides) cellulosolvens ATCC 35603.	Dassa B, Utturkar S, Hurt RA, Klingeman DM, Keller M, Xu J, Reddy YH, Borovok I, Rozman Grinberg I, Lamed R, Zhivin O, Bayer EA, Brown SD	Genome Announc	10.1128/genomeA.01022-15	2015	*
Phylogeny	Description of Anaerobacterium chartisolvens gen. nov., sp. nov., an obligately anaerobic bacterium from Clostridium rRNA cluster III isolated from soil of a Japanese rice field, and reclassification of Bacteroides cellulosolvens Murray et al. 1984 as Pseudobacteroides cellulosolvens gen. nov., comb. nov.	Horino H, Fujita T, Tonouchi A	Int J Syst Evol Microbiol	10.1099/ijs.0.059378-0	2014	*

References

#1253

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 2933

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68380

Automatically annotated from API rID32A .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#71226

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID41189.1 )

* These data were automatically processed and therefore are not curated

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Pseudobacteroides cellulosolvens ATCC 35603 = DSM 2933

Pseudobacteroides cellulosolvens ATCC 35603 = DSM 2933 DSM 2933

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