Peribacillus alkalitolerans (DSM 29135, KCTC 33631, MCCC 1K00503)

Name: Peribacillus alkalitolerans (DSM 29135, KCTC 33631, MCCC 1K00503)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 158068

Type strain

Strain Designation T3-209

Culture col. no. DSM 29135 KCTC 33631 MCCC 1K00503 T3-209

NCBI tax ID(s) 1550385

Special Collections DSMZ KCTC Literature strains

History <- X.-H. Zhang, Ocean Univ. China, Qingdao, P. R. China <- Y. Liu; T3-209 <- Xiao-Hua Zhang, Ocean Univ. of China

Links

Peribacillus alkalitolerans T3-209 is an aerobe, spore-forming, mesophilic prokaryote that forms circular colonies and was isolated from sediment, depth of 1206 m.

spore-forming Gram-positive motile rod-shaped colony-forming aerobe mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 406060

KCTC 33631,DSM 29135

@ref 20215

Last LPSN update 2026-02-09 11:38:13

Domain Bacteria

Phylum Bacillota

Class Bacilli

Order Caryophanales

Family Bacillaceae

Genus Peribacillus

Species Peribacillus alkalitolerans

Full scientific name Peribacillus alkalitolerans (Liu et al. 2018) Gupta et al. 2020

Synonyms (1)

Bacillus alkalitolerans

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape
65445	positive	2.6-3.5 µm	0.5 µm	rod-shaped
67771				rod-shaped
67771	positive

Colony morphology

@ref	Colony size	Colony color	Colony shape	Incubation period	Medium used
65445	1-2 mm	beige	circular	2 days	marine agar

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
64642	R2A MEDIUM (DSMZ Medium 830)		Medium recipe at MediaDive	Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water
65445	Marine agar (MA)

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
64642	positive	growth	28	mesophilic
65445	positive	growth	10-42
65445	positive	optimum	28	mesophilic
67771	positive	growth	28	mesophilic

Culture pH

@ref	Ability	Type	PH	PH range
65445	positive	optimum	8
65445	positive	growth	7-9	alkaliphile

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
65445	aerobe
67771	aerobe
125439	obligate aerobe	96.3

Spore formation

@ref	Spore description	Type of spore	Confidence
65445	terminal endospore-forming	endospore
67771		endospore
125439			93.4
125438			92.462

Halophily

@ref	Salt	Growth	Tested relation	Concentration
65445	NaCl	positive	growth	0-6 %(w/v)
65445	NaCl	positive	optimum	0 %(w/v)

Observation

@ref	Observation
65445	occur either singly or in chains
65445	the respiratory quinone is MK-7
65445	the polar lipid profile comprises phosphatidylglycerol, two unidentified phospholipids and one unidentified aminolipid
67771	quinones: MK-7

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
65445	16651 ChEBI	(S)-lactate	-	assimilation
65445	86537 ChEBI	2,2-dimethylsuccinic acid	-	assimilation
65445	62064 ChEBI	2,3-butanediol	-	assimilation
65445	17256 ChEBI	2-deoxyadenosine	-	assimilation
65445	64552 ChEBI	2-hydroxybutyrate	-	assimilation
65445	28644 ChEBI	2-oxopentanoate	+	assimilation
65445		3-O-methyl alpha-D-glucopyranoside	-	assimilation
65445	73918 ChEBI	3-O-methyl-D-glucose	-	assimilation
65445	16724 ChEBI	4-hydroxybutyrate	-	assimilation
65445	18101 ChEBI	4-hydroxyphenylacetic acid	-	assimilation
65445	355715 ChEBI	4-nitrophenyl beta-D-galactopyranoside	+	hydrolysis
65445	30089 ChEBI	acetate	+	assimilation
65445	16335 ChEBI	adenosine	-	assimilation
65445	40585 ChEBI	alpha-cyclodextrin	-	assimilation
65445	17665 ChEBI	alpha-D-glucose 6-phosphate	-	assimilation
65445	17665 ChEBI	alpha-D-glucose 6-phosphate	+	assimilation
65445	27613 ChEBI	amygdalin	-	assimilation
65445	27613 ChEBI	amygdalin	+	builds acid from
65445	18305 ChEBI	arbutin	-	assimilation
65445	18305 ChEBI	arbutin	+	builds acid from
65445	495055 ChEBI	beta-cyclodextrin	-	assimilation
65445	28034 ChEBI	beta-D-galactoside	-	assimilation
65445	71422 ChEBI	beta-gentiobiose	+	builds acid from
65445	8295 ChEBI	beta-hydroxybutyrate	-	assimilation
65445	85146 ChEBI	carboxymethylcellulose	-	hydrolysis
65445		casein	-	hydrolysis
65445	17057 ChEBI	cellobiose	-	assimilation
65445	17057 ChEBI	cellobiose	+	builds acid from
65445	15570 ChEBI	D-alanine	-	assimilation
65445	18333 ChEBI	D-arabitol	-	assimilation
65445	15824 ChEBI	D-fructose	-	assimilation
65445	15824 ChEBI	D-fructose	+	builds acid from
65445	78697 ChEBI	D-fructose 6-phosphate	-	assimilation
65445	18024 ChEBI	D-galacturonic acid	-	assimilation
65445	8391 ChEBI	D-gluconate	-	assimilation
65445	17634 ChEBI	D-glucose	+	builds acid from
65445	15588 ChEBI	D-malate	-	assimilation
65445	16899 ChEBI	D-mannitol	-	assimilation
65445	16899 ChEBI	D-mannitol	+	builds acid from
65445	16024 ChEBI	D-mannose	-	assimilation
65445	16024 ChEBI	D-mannose	+	builds acid from
65445	27605 ChEBI	D-psicose	-	assimilation
65445	16988 ChEBI	D-ribose	-	assimilation
65445	17924 ChEBI	D-sorbitol	-	assimilation
65445	16443 ChEBI	D-tagatose	-	assimilation
65445	65327 ChEBI	D-xylose	-	assimilation
65445	16991 ChEBI	dna	+	hydrolysis
65445	4853 ChEBI	esculin	+	hydrolysis
65445		esculin ferric citrate	+	builds acid from
65445	28260 ChEBI	galactose	-	assimilation
65445	495056 ChEBI	gamma-cyclodextrin	-	assimilation
65445	5291 ChEBI	gelatin	+	hydrolysis
65445	28066 ChEBI	gentiobiose	-	assimilation
65445	14336 ChEBI	glycerol 1-phosphate	-	assimilation
65445	15978 ChEBI	glycerol 3-phosphate	-	assimilation
65445	70744 ChEBI	glycine-proline	+	assimilation
65445	28087 ChEBI	glycogen	-	assimilation
65445	28087 ChEBI	glycogen	+	builds acid from
65445	73784 ChEBI	glycyl-l-glutamate	-	assimilation
65445	73784 ChEBI	glycyl-l-glutamate	+	assimilation
65445	17368 ChEBI	hypoxanthine	-	hydrolysis
65445	17596 ChEBI	inosine	-	assimilation
65445	15443 ChEBI	inulin	-	assimilation
65445	21217 ChEBI	L-alaninamide	-	assimilation
65445	73786 ChEBI	L-alanylglycine	-	assimilation
65445	30849 ChEBI	L-arabinose	-	assimilation
65445	30849 ChEBI	L-arabinose	+	builds acid from
65445	17196 ChEBI	L-asparagine	-	assimilation
65445	28120 ChEBI	L-fructose	-	assimilation
65445	15589 ChEBI	L-malate	+	assimilation
65445	18183 ChEBI	L-pyroglutamic acid	-	assimilation
65445	62345 ChEBI	L-rhamnose	-	assimilation
65445	17115 ChEBI	L-serine	+	assimilation
65445	75144 ChEBI	lactamide	-	assimilation
65445	17716 ChEBI	lactose	-	assimilation
65445	6359 ChEBI	lactulose	-	assimilation
65445	17306 ChEBI	maltose	+	builds acid from
65445		maltose hydrate	+	assimilation
65445	61993 ChEBI	maltotriose	+	assimilation
65445	28808 ChEBI	mannan	+	assimilation
65445	6731 ChEBI	melezitose	-	assimilation
65445	28053 ChEBI	melibiose	-	assimilation
65445	28053 ChEBI	melibiose	+	builds acid from
65445	55507 ChEBI	methyl alpha-D-galactoside	-	assimilation
65445	320061 ChEBI	methyl alpha-D-glucopyranoside	+	builds acid from
65445	43943 ChEBI	methyl alpha-D-mannoside	-	assimilation
65445	43943 ChEBI	methyl alpha-D-mannoside	+	builds acid from
65445	51850 ChEBI	methyl pyruvate	+	assimilation
65445	17268 ChEBI	myo-inositol	+	assimilation
65445	63153 ChEBI	N-acetyl-D-mannosamine	-	assimilation
65445	44337 ChEBI	N-acetyl-L-glutamate	-	assimilation
65445	506227 ChEBI	N-acetylglucosamine	-	assimilation
65445	506227 ChEBI	N-acetylglucosamine	+	builds acid from
65445	17632 ChEBI	nitrate	+	reduction
65445		O-nitrophenyl-beta-D-galactopyranosid	+	hydrolysis
65445	18394 ChEBI	palatinose	-	assimilation
65445	17272 ChEBI	propionate	+	assimilation
65445	17148 ChEBI	putrescine	-	assimilation
65445	16634 ChEBI	raffinose	-	assimilation
65445	33942 ChEBI	ribose	+	builds acid from
65445	17814 ChEBI	salicin	-	assimilation
65445	17814 ChEBI	salicin	+	builds acid from
65445	32488 ChEBI	sedoheptulosan	-	assimilation
65445	17164 ChEBI	stachyose	-	assimilation
65445	28017 ChEBI	starch	+	builds acid from
65445	28017 ChEBI	starch	+	hydrolysis
65445	17992 ChEBI	sucrose	+	assimilation
65445	17992 ChEBI	sucrose	+	builds acid from
65445	17748 ChEBI	thymidine	+	assimilation
65445	63528 ChEBI	thymidine 5'-monophosphate	-	assimilation
65445	27082 ChEBI	trehalose	-	assimilation
65445	27082 ChEBI	trehalose	+	builds acid from
65445	32528 ChEBI	turanose	-	assimilation
65445	32528 ChEBI	turanose	+	builds acid from
65445	53424 ChEBI	tween 20	+	hydrolysis
65445	53423 ChEBI	tween 40	+	assimilation
65445	53423 ChEBI	tween 40	+	hydrolysis
65445	53426 ChEBI	tween 80	+	assimilation
65445	53426 ChEBI	tween 80	+	hydrolysis
65445	18186 ChEBI	tyrosine	-	hydrolysis
65445	16704 ChEBI	uridine	+	assimilation
65445	16695 ChEBI	uridine 5'-monophosphate	-	assimilation
65445	15318 ChEBI	xanthine	-	hydrolysis
65445	17151 ChEBI	xylitol	-	assimilation

Antibiotic resistance

@ref	ChEBI	Metabolite	Sensitivity conc.	Resistance conc.
65445	28971	ampicillin	10 µg (disc)
65445	17698	chloramphenicol	30 µg (disc)
65445	6104	kanamycin	30 µg (disc)
65445	18208	penicillin g		10 µg
65445	8309	polymyxin b		300 Unit
65445	28077	rifampicin	5 µg (disc)
65445	9215	spectinomycin	100 µg (disc)
65445	17076	streptomycin		10 µg
65445	27902	tetracycline	30 µg (disc)

Enzymes

@ref	Value	Activity	Ec
65445	acid phosphatase	+	3.1.3.2
65445	alkaline phosphatase	+	3.1.3.1
65445	alpha-chymotrypsin	+	3.4.21.1
65445	alpha-fucosidase	-	3.2.1.51
65445	alpha-galactosidase	-	3.2.1.22
65445	alpha-glucosidase	+	3.2.1.20
65445	alpha-mannosidase	-	3.2.1.24
65445	beta-galactosidase	+	3.2.1.23
65445	beta-glucosidase	-	3.2.1.21
65445	beta-glucuronidase	-	3.2.1.31
65445	catalase	+	1.11.1.6
65445	cystine arylamidase	+	3.4.11.3
65445	cytochrome oxidase	+	1.9.3.1
65445	esterase	+
65445	esterase Lipase (C 8)	+
65445	leucine arylamidase	-	3.4.11.1
65445	lipase (C 14)	-
65445	N-acetyl-beta-glucosaminidase	+	3.2.1.52
65445	naphthol-AS-BI-phosphohydrolase	+
65445	trypsin	-	3.4.21.4
65445	valine arylamidase	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Hydrothermal vent
#Environmental	#Aquatic	#Sediment

Isolation

@ref	Sample type	Sampling date	Geographic location	Country	Country ISO 3 Code	Continent	Latitude	Longitude	Enrichment culture temperature	Isolation procedure
64642	sediment, depth of 1206 m		Okinawa Trough	China	CHN	Asia
65445	sediment sampled at a water depth of 1206 m of the southern Okinawa Trough at station T1 near the Tangyin hydrothermal vent during an expedition on the R/V Kexue	May 2014	southern Okinawa Trough	China	CHN	Asia	25.07	122.58 25.07/122.58	28	was isolated by means of a standard dilution plating technique and a pure culture was obtained by repeated transfers of separate colonies on marine agar 2216 (MA; g Bacto peptone, 1g yeast extract, 0.01g FePO4 and 20g agar in 1l seawater)
67771	From marine sediment, depth of 1206 m		Okinawa Trough	China	CHN	Asia

Interaction and safety

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM1088212v1 assembly for Peribacillus alkalitolerans KCTC 33631	scaffold	GCA_010882125	1550385.3	2964380400	1550385	62.32

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
64642	Peribacillus alkalitolerans strain T3-209 16S ribosomal RNA gene, partial sequence	KM077161	1514		1550385

GC content

@ref	GC-content (mol%)	Method
64642	41.8
65445	41.8	high performance liquid chromatography (HPLC)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Peribacillus alkalitolerans KCTC 33631
NCBI: GCA_010882125

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	yes	93.40	no
125439	motility	BacteriaNetⓘ	yes	84.30	no
125439	gram_stain	BacteriaNetⓘ	variable	80.70	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	96.30	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Bacillus alkalitolerans KCTC 33631
NCBI: GCA_010882125.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	79.29	yes
125438	anaerobic	anaerobicⓘ	no	96.80	no
125438	aerobic	aerobicⓘ	yes	81.60	yes
125438	spore-forming	spore-formingⓘ	yes	92.46	no
125438	thermophilic	thermophileⓘ	no	88.34	yes
125438	flagellated	motile2+ⓘ	yes	79.61	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Transfer of Bacillus tepidiphilus Narsing Rao et al. 2021 to the genus Peribacillus as Peribacillus tepidiphilus comb. nov.	Narsing Rao MP, Dhulappa A, Banerjee A, Thamchaipenet A	Arch Microbiol	10.1007/s00203-022-03160-w	2022
Phylogeny	Bacillus alkalitolerans sp. nov., isolated from marine sediment near a hydrothermal vent.	Liu Y, Yu M, Zhang XH	Int J Syst Evol Microbiol	10.1099/ijsem.0.002648	2018

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#64642	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 29135
#65445	Yan Liu, Min Yu, Xiao-Hua Zhang: Bacillus alkalitolerans sp. nov., isolated from marine sediment near a hydrothermal vent. IJSEM 68: 1184 - 1189 2018 ( DOI 10.1099/ijsem.0.002648 , PubMed 29465340 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67771	Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Peribacillus alkalitolerans (DSM 29135, KCTC 33631, MCCC 1K00503)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Observation

Metabolite utilization

Antibiotic resistance

Enzymes

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Sequence information

Genome sequences

Genome browser: GCA_010882125

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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