Name: Peribacillus alkalitolerans T3-209
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 158068

Type strain:

Species: Peribacillus alkalitolerans

Strain Designation: T3-209

Culture col. no.: DSM 29135, KCTC 33631, MCCC 1K00503

Strain history: <- Xiao-Hua Zhang, Ocean Univ. of China

NCBI tax ID(s): 1550385 (species)

SI-ID 406060

DSM 29135

Special Collections

Section

Peribacillus alkalitolerans T3-209 is an aerobe, spore-forming, Gram-positive bacterium that forms circular colonies and was isolated from sediment, depth of 1206 m.

colony-forming
Gram-positive
motile
rod-shaped
spore-forming
aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Peribacillus*
	Species	**Peribacillus alkalitolerans**
	Full Scientific Name (LPSN)	Peribacillus alkalitolerans (Liu et al. 2018) Gupta et al. 2020

Synonym

Bacillus alkalitolerans

Information on morphological and physiological properties Morphology

[Ref.: #65445]	Gram stain	positive
[Ref.: #67771]	Gram stain	positive

[Ref.: #65445]	Cell length	2.6-3.5 µm

[Ref.: #65445]	Cell width	0.5 µm

[Ref.: #65445]	Cell shape	rod-shaped
[Ref.: #67771]	Cell shape	rod-shaped

[Ref.: #65445]	Motility	yes
[Ref.: #67771]	Motility	yes

[Ref.: #65445]	Colony size	1-2 mm
[Ref.: #65445]	Colony color	beige
[Ref.: #65445]	Colony shape	circular
[Ref.: #65445]	Incubation period	2 days
[Ref.: #65445]	Cultivation medium used	marine agar

Information on culture and growth conditions Culture and growth conditions

[Ref.: #64642]

Culture medium

R2A MEDIUM (DSMZ Medium 830)

[Ref.: #64642]

Culture medium growth

[Ref.: #64642]

Culture medium link

Medium recipe at MediaDive

[Ref.: #64642]

Culture medium composition

expand / minimize

[Ref.: #65445]

Culture medium

Marine agar (MA)

[Ref.: #65445]

Culture medium growth

		Temperature
[Ref.: #64642]	growth	28 °C
[Ref.: #65445]	growth	10-42 °C
[Ref.: #65445]	optimum	28 °C
[Ref.: #67771]	growth	28 °C

	pH	Kind of pH		pH
[Ref.: #65445]			optimum	8
[Ref.: #65445]			growth	7-9

Information on physiology and metabolism Physiology and metabolism

[Ref.: #65445]	Oxygen tolerance	aerobe
[Ref.: #67771]	Oxygen tolerance	aerobe

[Ref.: #65445]	Spore description	terminal endospore-forming
[Ref.: #65445]	Type of spore	endospore
[Ref.: #65445]	Ability of spore formation	yes
[Ref.: #67771]	Type of spore	endospore
[Ref.: #67771]	Ability of spore formation	yes
[Ref.: #69480]	Ability of spore formation	yes Confidence in %92.462
[Ref.: #69481]	Ability of spore formation	yes Confidence in %100

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #65445]		NaCl		growth	0-6	%(w/v)
[Ref.: #65445]		NaCl		optimum	0	%(w/v)

[Ref.: #65445]	Observation	occur either singly or in chains
[Ref.: #65445]	Observation	the respiratory quinone is MK-7
[Ref.: #65445]	Observation	the polar lipid profile comprises phosphatidylglycerol, two unidentified phospholipids and one unidentified aminolipid
[Ref.: #67771]	Observation	quinones: MK-7

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #65445]	(S)-lactate ChEBI	-	assimilation
[Ref.: #65445]	2,2-dimethylsuccinic acid ChEBI	-	assimilation
[Ref.: #65445]	2,3-butanediol ChEBI	-	assimilation
[Ref.: #65445]	2-deoxyadenosine ChEBI	-	assimilation
[Ref.: #65445]	2-hydroxybutyrate ChEBI	-	assimilation
[Ref.: #65445]	2-oxopentanoate ChEBI	+	assimilation
[Ref.: #65445]	3-O-methyl alpha-D-glucopyranoside	-	assimilation
[Ref.: #65445]	3-O-methyl-D-glucose ChEBI	-	assimilation
[Ref.: #65445]	4-hydroxybutyrate ChEBI	-	assimilation
[Ref.: #65445]	4-hydroxyphenylacetic acid ChEBI	-	assimilation
[Ref.: #65445]	4-nitrophenyl beta-D-galactopyranoside ChEBI	+	hydrolysis
[Ref.: #65445]	acetate ChEBI	+	assimilation
[Ref.: #65445]	adenosine ChEBI	-	assimilation
[Ref.: #65445]	alpha-cyclodextrin ChEBI	-	assimilation
[Ref.: #65445]	alpha-D-glucose 6-phosphate ChEBI	-	assimilation
[Ref.: #65445]	alpha-D-glucose 6-phosphate ChEBI	+	assimilation
[Ref.: #65445]	amygdalin ChEBI	-	assimilation
[Ref.: #65445]	amygdalin ChEBI	+	builds acid from
[Ref.: #65445]	arbutin ChEBI	-	assimilation
[Ref.: #65445]	arbutin ChEBI	+	builds acid from
[Ref.: #65445]	beta-cyclodextrin ChEBI	-	assimilation
[Ref.: #65445]	beta-D-galactoside ChEBI	-	assimilation
[Ref.: #65445]	beta-gentiobiose ChEBI	+	builds acid from
[Ref.: #65445]	beta-hydroxybutyrate ChEBI	-	assimilation
[Ref.: #65445]	carboxymethylcellulose ChEBI	-	hydrolysis
[Ref.: #65445]	casein	-	hydrolysis
[Ref.: #65445]	cellobiose ChEBI	-	assimilation
[Ref.: #65445]	cellobiose ChEBI	+	builds acid from
[Ref.: #65445]	D-alanine ChEBI	-	assimilation
[Ref.: #65445]	D-arabitol ChEBI	-	assimilation
[Ref.: #65445]	D-fructose ChEBI	-	assimilation
[Ref.: #65445]	D-fructose ChEBI	+	builds acid from
[Ref.: #65445]	D-fructose 6-phosphate ChEBI	-	assimilation
[Ref.: #65445]	D-galacturonic acid ChEBI	-	assimilation
[Ref.: #65445]	D-gluconate ChEBI	-	assimilation
[Ref.: #65445]	D-glucose ChEBI	+	builds acid from
[Ref.: #65445]	D-malate ChEBI	-	assimilation
[Ref.: #65445]	D-mannitol ChEBI	-	assimilation
[Ref.: #65445]	D-mannitol ChEBI	+	builds acid from
[Ref.: #65445]	D-mannose ChEBI	-	assimilation
[Ref.: #65445]	D-mannose ChEBI	+	builds acid from
[Ref.: #65445]	D-psicose ChEBI	-	assimilation
[Ref.: #65445]	D-ribose ChEBI	-	assimilation
[Ref.: #65445]	D-sorbitol ChEBI	-	assimilation
[Ref.: #65445]	D-tagatose ChEBI	-	assimilation
[Ref.: #65445]	D-xylose ChEBI	-	assimilation
[Ref.: #65445]	dna ChEBI	+	hydrolysis
[Ref.: #65445]	esculin ChEBI	+	hydrolysis
[Ref.: #65445]	esculin ferric citrate	+	builds acid from
[Ref.: #65445]	galactose ChEBI	-	assimilation
[Ref.: #65445]	gamma-cyclodextrin ChEBI	-	assimilation
[Ref.: #65445]	gelatin ChEBI	+	hydrolysis
[Ref.: #65445]	gentiobiose ChEBI	-	assimilation
[Ref.: #65445]	glycerol 1-phosphate ChEBI	-	assimilation
[Ref.: #65445]	glycerol 3-phosphate ChEBI	-	assimilation
[Ref.: #65445]	glycine-proline ChEBI	+	assimilation
[Ref.: #65445]	glycogen ChEBI	-	assimilation
[Ref.: #65445]	glycogen ChEBI	+	builds acid from
[Ref.: #65445]	glycyl-l-glutamate ChEBI	-	assimilation
[Ref.: #65445]	glycyl-l-glutamate ChEBI	+	assimilation
[Ref.: #65445]	hypoxanthine ChEBI	-	hydrolysis
[Ref.: #65445]	inosine ChEBI	-	assimilation
[Ref.: #65445]	inulin ChEBI	-	assimilation
[Ref.: #65445]	L-alaninamide ChEBI	-	assimilation
[Ref.: #65445]	L-alanylglycine ChEBI	-	assimilation
[Ref.: #65445]	L-arabinose ChEBI	-	assimilation
[Ref.: #65445]	L-arabinose ChEBI	+	builds acid from
[Ref.: #65445]	L-asparagine ChEBI	-	assimilation
[Ref.: #65445]	L-fructose ChEBI	-	assimilation
[Ref.: #65445]	L-malate ChEBI	+	assimilation
[Ref.: #65445]	L-pyroglutamic acid ChEBI	-	assimilation
[Ref.: #65445]	L-rhamnose ChEBI	-	assimilation
[Ref.: #65445]	L-serine ChEBI	+	assimilation
[Ref.: #65445]	lactamide ChEBI	-	assimilation
[Ref.: #65445]	lactose ChEBI	-	assimilation
[Ref.: #65445]	lactulose ChEBI	-	assimilation
[Ref.: #65445]	maltose ChEBI	+	builds acid from
[Ref.: #65445]	maltose hydrate	+	assimilation
[Ref.: #65445]	maltotriose ChEBI	+	assimilation
[Ref.: #65445]	mannan ChEBI	+	assimilation
[Ref.: #65445]	melezitose ChEBI	-	assimilation
[Ref.: #65445]	melibiose ChEBI	-	assimilation
[Ref.: #65445]	melibiose ChEBI	+	builds acid from
[Ref.: #65445]	methyl alpha-D-galactoside ChEBI	-	assimilation
[Ref.: #65445]	methyl alpha-D-glucopyranoside ChEBI	+	builds acid from
[Ref.: #65445]	methyl alpha-D-mannoside ChEBI	-	assimilation
[Ref.: #65445]	methyl alpha-D-mannoside ChEBI	+	builds acid from
[Ref.: #65445]	methyl pyruvate ChEBI	+	assimilation
[Ref.: #65445]	myo-inositol ChEBI	+	assimilation
[Ref.: #65445]	N-acetyl-D-mannosamine ChEBI	-	assimilation
[Ref.: #65445]	N-acetyl-L-glutamate ChEBI	-	assimilation
[Ref.: #65445]	N-acetylglucosamine ChEBI	-	assimilation
[Ref.: #65445]	N-acetylglucosamine ChEBI	+	builds acid from
[Ref.: #65445]	nitrate ChEBI	+	reduction
[Ref.: #65445]	O-nitrophenyl-beta-D-galactopyranosid	+	hydrolysis
[Ref.: #65445]	palatinose ChEBI	-	assimilation
[Ref.: #65445]	propionate ChEBI	+	assimilation
[Ref.: #65445]	putrescine ChEBI	-	assimilation
[Ref.: #65445]	raffinose ChEBI	-	assimilation
[Ref.: #65445]	ribose ChEBI	+	builds acid from
[Ref.: #65445]	salicin ChEBI	-	assimilation
[Ref.: #65445]	salicin ChEBI	+	builds acid from
[Ref.: #65445]	sedoheptulosan ChEBI	-	assimilation
[Ref.: #65445]	stachyose ChEBI	-	assimilation
[Ref.: #65445]	starch ChEBI	+	builds acid from
[Ref.: #65445]	starch ChEBI	+	hydrolysis
[Ref.: #65445]	sucrose ChEBI	+	assimilation
[Ref.: #65445]	sucrose ChEBI	+	builds acid from
[Ref.: #65445]	thymidine ChEBI	+	assimilation
[Ref.: #65445]	thymidine 5'-monophosphate ChEBI	-	assimilation
[Ref.: #65445]	trehalose ChEBI	-	assimilation
[Ref.: #65445]	trehalose ChEBI	+	builds acid from
[Ref.: #65445]	turanose ChEBI	-	assimilation
[Ref.: #65445]	turanose ChEBI	+	builds acid from
[Ref.: #65445]	tween 20 ChEBI	+	hydrolysis
[Ref.: #65445]	tween 40 ChEBI	+	assimilation
[Ref.: #65445]	tween 40 ChEBI	+	hydrolysis
[Ref.: #65445]	tween 80 ChEBI	+	assimilation
[Ref.: #65445]	tween 80 ChEBI	+	hydrolysis
[Ref.: #65445]	tyrosine ChEBI	-	hydrolysis
[Ref.: #65445]	uridine ChEBI	+	assimilation
[Ref.: #65445]	uridine 5'-monophosphate ChEBI	-	assimilation
[Ref.: #65445]	xanthine ChEBI	-	hydrolysis
[Ref.: #65445]	xylitol ChEBI	-	assimilation
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Concentration		Antibiotic resistance	Concentration
[Ref.: #65445]	ampicillin ChEBI	yes	10	µg (disc)
[Ref.: #65445]	chloramphenicol ChEBI	yes	30	µg (disc)
[Ref.: #65445]	kanamycin ChEBI	yes	30	µg (disc)
[Ref.: #65445]	penicillin g ChEBI				yes	10	µg
[Ref.: #65445]	polymyxin b ChEBI				yes	300	Unit
[Ref.: #65445]	rifampicin ChEBI	yes	5	µg (disc)
[Ref.: #65445]	spectinomycin ChEBI	yes	100	µg (disc)
[Ref.: #65445]	streptomycin ChEBI				yes	10	µg
[Ref.: #65445]	tetracycline ChEBI	yes	30	µg (disc)

	Enzyme	Enzyme activity	EC number
[Ref.: #65445]	acid phosphatase	+	3.1.3.2
[Ref.: #65445]	alkaline phosphatase	+	3.1.3.1
[Ref.: #65445]	alpha-chymotrypsin	+	3.4.21.1
[Ref.: #65445]	alpha-fucosidase	-	3.2.1.51
[Ref.: #65445]	alpha-galactosidase	-	3.2.1.22
[Ref.: #65445]	alpha-glucosidase	+	3.2.1.20
[Ref.: #65445]	alpha-mannosidase	-	3.2.1.24
[Ref.: #65445]	beta-galactosidase	+	3.2.1.23
[Ref.: #65445]	beta-glucosidase	-	3.2.1.21
[Ref.: #65445]	beta-glucuronidase	-	3.2.1.31
[Ref.: #65445]	catalase	+	1.11.1.6
[Ref.: #65445]	cystine arylamidase	+	3.4.11.3
[Ref.: #65445]	cytochrome oxidase	+	1.9.3.1
[Ref.: #65445]	esterase	+
[Ref.: #65445]	esterase Lipase (C 8)	+
[Ref.: #65445]	leucine arylamidase	-	3.4.11.1
[Ref.: #65445]	lipase (C 14)	-
[Ref.: #65445]	N-acetyl-beta-glucosaminidase	+	3.2.1.52
[Ref.: #65445]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #65445]	trypsin	-	3.4.21.4
[Ref.: #65445]	valine arylamidase	-
	Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #64642]	Sample type/isolated from	sediment, depth of 1206 m
[Ref.: #64642]	Geographic location (country and/or sea, region)	Okinawa Trough
[Ref.: #64642]	Country	China
[Ref.: #64642]	Country ISO 3 Code	CHN
[Ref.: #64642]	Continent	Asia

[Ref.: #65445]	Sample type/isolated from	sediment sampled at a water depth of 1206 m of the southern Okinawa Trough at station T1 near the Tangyin hydrothermal vent during an expedition on the R/V Kexue
[Ref.: #65445]	Sampling date	May 2014
[Ref.: #65445]	Geographic location (country and/or sea, region)	southern Okinawa Trough
[Ref.: #65445]	Country	China
[Ref.: #65445]	Country ISO 3 Code	CHN
[Ref.: #65445]	Continent	Asia
[Ref.: #65445]	Geographic location	25.0700°/122.5800°
[Ref.: #65445]	Enrichment culture temperature	28 ̊C
[Ref.: #65445]	Isolation/enrichment procedure	was isolated by means of a standard dilution plating technique and a pure culture was obtained by repeated transfers of separate colonies on marine agar 2216 (MA; g Bacto peptone, 1g yeast extract, 0.01g FePO4 and 20g agar in 1l seawater)

[Ref.: #67771]	Sample type/isolated from	From marine sediment, depth of 1206 m
[Ref.: #67771]	Geographic location (country and/or sea, region)	Okinawa Trough
[Ref.: #67771]	Country	China
[Ref.: #67771]	Country ISO 3 Code	CHN
[Ref.: #67771]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Hydrothermal vent
#Environmental	#Aquatic	#Sediment

What are isolation sources categories?

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #64642]	Peribacillus alkalitolerans strain T3-209 16S ribosomal RNA gene, partial sequence	KM077161	1514	GenBank ENA	1550385 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Peribacillus alkalitolerans KCTC 33631	GCA_010882125	scaffold	GenBank ENA	1550385 tax ID	*
[Ref.: #66792]	Bacillus alkalitolerans strain KCTC 33631	1550385.3	wgs	PATRIC	1550385 tax ID	*

[Ref.: #64642]	GC-content	41.8 mol%
[Ref.: #65445]	GC-content	41.8 mol%	high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Bacillus alkalitolerans KCTC 33631 NCBI: GCA_010882125.1
[Ref.: #69481]	spore-forming	spore-formingⓘ	yes	100	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Bacillus alkalitolerans KCTC 33631 NCBI: GCA_010882125.1
[Ref.: #69480]	gram-positive	gram-positiveⓘ	yes	79.294	yes
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	96.795	no
[Ref.: #69480]	aerobic	aerobicⓘ	yes	81.599	yes
[Ref.: #69480]	spore-forming	spore-formingⓘ	yes	92.462	no
[Ref.: #69480]	thermophilic	thermophileⓘ	no	88.343	yes
[Ref.: #69480]	flagellated	motile2+ⓘ	yes	79.611	no

Availability in culture collections External links

[Ref.: #64642]

Culture collection no.

DSM 29135, KCTC 33631, MCCC 1K00503

[Ref.: #110912]

SI-ID 406060

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Bacillus alkalitolerans sp. nov., isolated from marine sediment near a hydrothermal vent.	Liu Y, Yu M, Zhang XH	Int J Syst Evol Microbiol	10.1099/ijsem.0.002648	2018	*
Phylogeny	Transfer of Bacillus tepidiphilus Narsing Rao et al. 2021 to the genus Peribacillus as Peribacillus tepidiphilus comb. nov.	Narsing Rao MP, Dhulappa A, Banerjee A, Thamchaipenet A	Arch Microbiol	10.1007/s00203-022-03160-w	2022	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#64642

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 29135

#65445

Yan Liu, Min Yu, Xiao-Hua Zhang: Bacillus alkalitolerans sp. nov., isolated from marine sediment near a hydrothermal vent. IJSEM 68: 1184 - 1189 2018 ( DOI 10.1099/ijsem.0.002648 , PubMed 29465340 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#110912

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID406060.1 )

* These data were automatically processed and therefore are not curated

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Bacillus alkalitolerans KCTC 33631

Bacillus alkalitolerans KCTC 33631

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