Pseudomonas asiatica (DSM 107182, JCM 32716, RYU-5)

Name: Pseudomonas asiatica (DSM 107182, JCM 32716, RYU-5)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 157812

Type strain

Strain Designation RYU-5, RYU5

Culture col. no. DSM 107182 JCM 32716 RYU-5 RYU5

NCBI tax ID(s) 2219225

Special Collections DSMZ JCM Literature strains

History <- M. Tohya, Juntendo Univerity of School of Medicine Department of Microbiology; RYU5 <- K. Uechi; RYU5 M. Tohya; Juntendo Univ. Sch. of Med., Japan; RYU5.

Links

Pseudomonas asiatica RYU-5 is an aerobe, mesophilic, Gram-negative prokaryote that forms circular colonies and has multiple antibiotic resistances.

antibiotic resistance Gram-negative motile rod-shaped colony-forming aerobe mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 402840

JCM32716,DSM 107182

@ref 20215

Last LPSN update 2026-02-09 11:38:09

Domain Bacteria

Phylum Pseudomonadota

Class Gammaproteobacteria

Order Pseudomonadales

Family Pseudomonadaceae

Genus Pseudomonas

Species Pseudomonas asiatica

Full scientific name Pseudomonas asiatica Tohya et al. 2019

Synonyms (1)

Pseudomonas shirazica

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Confidence
67868	negative	2-2.5 µm	0.5-1 µm	rod-shaped
125439	negative				98.8
125438	negative				99

Colony morphology

@ref	Colony size	Colony shape	Incubation period	Medium used
67868	0.5-2.5 mm	circular	2 days	Trypticase Soy Agar (TSA)

Pigmentation

@ref	Production	Name
67868		fluorescent pigment

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
64384	COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)		Medium recipe at MediaDive	Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
64384	TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)		Medium recipe at MediaDive	Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
64384	positive	growth	30	mesophilic
67770	positive	growth	30	mesophilic
67868	positive	growth	4-40
67868	positive	optimum	28-36	mesophilic

Culture pH

@ref	Ability	Type	PH	PH range
67868	positive	optimum	7-8
67868	positive	growth	6-9	alkaliphile

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
67868	aerobe
125438	aerobe	93.521

Antibiotic susceptibility testing

	Test 1	Test 2
@ref	64384	64384
Medium	Mueller-Hinton Agar	Mueller-Hinton Agar
Manual annotation	1	1
	Inhibition zone diameter in mm	Inhibition zone diameter in mm
Amikacin 30µg (disc)	22-24	22-24
Ampicillin 10µg (disc)	0	0
Aztreonam 30µg (disc)	18	18
Bacitracin 10Unit	0	0
Cefalotin 30µg (disc)	0	0
Cefazolin 30µg (disc)	0	0
Cefotaxime 30µg (disc)	0	0
Ceftriaxone 30µg (disc)	6	6
Chloramphenicol 30µg (disc)	0	0
Clindamycin 10µg (disc)	0	0
Colistin 10µg (disc)	14	14
Doxycycline 30µg (disc)	20	20
Erythromycin 15µg (disc)	0	0
Fosfomycin 50µg (disc)	0	0
Gentamycin 10µg (disc)
Imipenem 10µg (disc)	10	10
Kanamycin 30µg (disc)	28	28
Lincomycin 15µg (disc)	0	0
Linezolid 10µg (disc)	0	0
Mezlocillin 30µg (disc)	8	8
Moxifloxacin 5µg (disc)	26	26
Neomycin 30µg (disc)	20-22	20-22
Nitrofurantoin 100µg (disc)	0	0
Norfloxacin 10µg (disc)	30	30
Nystatin 100Unit	0	0
Ofloxacin 5µg (disc)	22-24	22-24
Oxacillin 5µg (disc)	0	0
Penicillin G 6µg (disc)	0	0
Pipemidic acid 20µg (disc)	18	18
Piperacillin/Tazobactam 40µg (disc)	16	16
Polymyxin B 300Unit	16	16
Quinupristin/Dalfopristin 15µg (disc)	0	0
Teicoplanin 30µg (disc)	0	0
Tetracycline 30µg (disc)	20	20
Ticarcillin 75µg (disc)	0	0
Vancomycin 30µg (disc)	0	0

Spore formation

@ref	Spore formation	Confidence
67868
125439		98.7

Halophily

@ref	Salt	Growth	Tested relation	Concentration
67868	NaCl	positive	growth	0-5.5 %(w/v)
67868	NaCl	positive	growth	0-3 %
67868	NaCl	positive	growth	8 %

Observation

67868

ObservationFluorescent pigment is produced on King?s B agar at 30°C.

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
67868	16651 ChEBI	(S)-lactate	+	carbon source
67868	16810 ChEBI	2-oxoglutarate	+	carbon source
67868	37054 ChEBI	3-hydroxybutyrate	+	carbon source
67868	30089 ChEBI	acetate	+	carbon source
67868	17128 ChEBI	adipate	+	assimilation
67868	17925 ChEBI	alpha-D-glucose	+	carbon source
68369	29016 ChEBI	arginine	+	hydrolysis	from API 20NE
67868	16947 ChEBI	citrate	+	carbon source
67868	15824 ChEBI	D-fructose	+	carbon source
67868	28847 ChEBI	D-fucose	+	carbon source
67868	30612 ChEBI	D-glucarate	+	carbon source
67868	8391 ChEBI	D-gluconate	+	carbon source
68369	17634 ChEBI	D-glucose	+	assimilation	from API 20NE
68369	17634 ChEBI	D-glucose	-	fermentation	from API 20NE
67868	16024 ChEBI	D-mannose	+	carbon source
68369	16024 ChEBI	D-mannose	+	assimilation	from API 20NE
67868	16523 ChEBI	D-serine	+	carbon source
67868	27689 ChEBI	decanoate	+	assimilation
68369	27689 ChEBI	decanoate	+	assimilation	from API 20NE
68369	4853 ChEBI	esculin	-	hydrolysis	from API 20NE
67868	15740 ChEBI	formate	+	carbon source
67868	16537 ChEBI	galactarate	+	carbon source
67868	16865 ChEBI	gamma-aminobutyric acid	+	carbon source
68369	5291 ChEBI	gelatin	-	hydrolysis	from API 20NE
68369	24265 ChEBI	gluconate	+	assimilation	from API 20NE
67868	32323 ChEBI	glucuronamide	+	carbon source
67868	17754 ChEBI	glycerol	+	carbon source
67868	17596 ChEBI	inosine	+	carbon source
67868	16977 ChEBI	L-alanine	+	carbon source
68369	30849 ChEBI	L-arabinose	+	assimilation	from API 20NE
67868	16467 ChEBI	L-arginine	+	carbon source
67868	29991 ChEBI	L-aspartate	+	carbon source
67868	29988 ChEBI	L-glutamate	+	carbon source
67868	15971 ChEBI	L-histidine	+	carbon source
67868	15589 ChEBI	L-malate	+	carbon source
67868	57606 ChEBI	L-pyroglutamate	+	carbon source
67868	17115 ChEBI	L-serine	+	carbon source
67868	16828 ChEBI	L-tryptophan	+	assimilation
67868	25115 ChEBI	malate	+	assimilation
68369	25115 ChEBI	malate	+	assimilation	from API 20NE
68369	17632 ChEBI	nitrate	-	reduction	from API 20NE
67868	18401 ChEBI	phenylacetate	+	assimilation
67868	32032 ChEBI	potassium gluconate	+	assimilation
67868	17272 ChEBI	propionate	+	carbon source
67868	26490 ChEBI	quinate	+	carbon source
67868	53258 ChEBI	sodium citrate	+	assimilation
68369	27897 ChEBI	tryptophan	-	energy source	from API 20NE
68369	16199 ChEBI	urea	-	hydrolysis	from API 20NE

Antibiotic resistance

@ref	ChEBI	Metabolite	Resistance conc.	Sensitivity conc.
67868	2637	amikacin		<4 µg/mL
64384	28971	Ampicillin	10 µg (disc)		from Antibiotic test
64384	28669	Bacitracin	10 Unit		from Antibiotic test
64384	124991	Cefalotin	30 µg (disc)		from Antibiotic test
64384	474053	Cefazolin	30 µg (disc)		from Antibiotic test
67868	478164	cefepime		16 µg/mL
64384	204928	Cefotaxime	30 µg (disc)		from Antibiotic test
67868	3508	ceftazidime		32 µg/mL
64384	17698	Chloramphenicol	30 µg (disc)		from Antibiotic test
67868	100241	ciprofloxacin		<0.5 µg/mL
64384	3745	Clindamycin	10 µg (disc)		from Antibiotic test
67868	37943	colistin		<0.5 µg/mL
64384	48923	Erythromycin	15 µg (disc)		from Antibiotic test
64384	28915	Fosfomycin	50 µg (disc)		from Antibiotic test
67868	17833	gentamicin		1 µg/mL
67868	471744	imipenem		16 µg/mL
64384	6472	Lincomycin	15 µg (disc)		from Antibiotic test
64384	63607	Linezolid	10 µg (disc)		from Antibiotic test
67868	43968	meropenem		32 µg/mL
64384	71415	Nitrofurantoin	100 µg (disc)		from Antibiotic test
64384	7660	Nystatin	100 Unit		from Antibiotic test
64384	7809	Oxacillin	5 µg (disc)		from Antibiotic test
64384	18208	Penicillin G	6 µg (disc)		from Antibiotic test
64384		Quinupristin/Dalfopristin	15 µg (disc)		from Antibiotic test
64384	29687	Teicoplanin	30 µg (disc)		from Antibiotic test
64384	9587	Ticarcillin	75 µg (disc)		from Antibiotic test
67868	28864	tobramycin		<0.5 µg/mL
64384	28001	Vancomycin	30 µg (disc)		from Antibiotic test

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68369	35581 ChEBI	indole		from API 20NE

Metabolite tests

@ref	Chebi-ID	Metabolite	Indole test
68369	35581 ChEBI	indole	-	from API 20NE

Enzymes

@ref	Value	Activity	Ec
67868	acid phosphatase	+	3.1.3.2
67868	alkaline phosphatase	+	3.1.3.1
68369	arginine dihydrolase	+	3.5.3.6	from API 20NE
68369	beta-glucosidase	-	3.2.1.21	from API 20NE
67868	catalase	+	1.11.1.6
67868	cytochrome oxidase	+	1.9.3.1
68369	cytochrome oxidase	+	1.9.3.1	from API 20NE
67868	esterase (C 4)	+
67868	esterase Lipase (C 8)	+
68369	gelatinase	-		from API 20NE
67868	leucine arylamidase	+	3.4.11.1
67868	naphthol-AS-BI-phosphohydrolase	+
68369	urease	-	3.5.1.5	from API 20NE

API 20NE

@ref	Reduction of nitratesNO3	TRP	GLU_ Ferm	ADH (Arg)	URE	ESC	GEL	PNPG	GLU_ Assim	ARA	MNE	MAN	NAG	MAL	GNT	CAP	ADI	MLT	CIT	PAC	OX
64384	-	-	-	+	-	-	-	-	+	+	+	+/-	+/-	+/-	+	+	+/-	+	+	+	+

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host Body Product	#Gastrointestinal tract	#Feces (Stool)
#Infection	#Patient	-

Isolation

@ref	Sample type	Sampling date	Geographic location	Country	Country ISO 3 Code	Continent	Enrichment culture
64384	faecal sample of patient with diarrhoea		Division of Clinical Laboratory and Blood Transfusion, University Hospital of the Ryukyus, Okinawa, Japan	Japan	JPN	Asia
67770	Stool sample of a patient hospitalized with diarrhoea at the University of the Ryukyus Hospital		Okinawa	Japan	JPN	Asia
67868	stool samples of male and female patients hospitalized with diarrhoea	2013-07	University of the Ryukyus Hospital, Okinawa	Japan	JPN	Asia	blood agar

Taxonmaps

69479

Global distribution of 16S sequence MH517510 (>99% sequence identity) for Pseudomonas from Microbeatlas

Aquatic Samples	543
Soil Samples	368
Animal Samples	799
Plant Samples	423
Total Samples	2133

Interaction and safety

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67868	ASM993233v1 assembly for Pseudomonas asiatica RYU5	contig	GCA_009932335	2219225.11	8035111415	2219225	79.21

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
64384	Pseudomonas sp. strain RYU5 16S ribosomal RNA gene, partial sequence	MH517510	1540		306

GC content

@ref	GC-content (mol%)	Method
64384	62.25	sequence analysis
67868	62.25	genome sequence analysis

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Pseudomonas asiatica RYU5
NCBI: GCA_009932335

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	98.70	no
125439	motility	BacteriaNetⓘ	yes	89.70	no
125439	gram_stain	BacteriaNetⓘ	negative	98.80	no
125439	oxygen_tolerance	BacteriaNetⓘ	aerobe	83.30	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Pseudomonas asiatica strain RYU5
PATRIC: 2219225.11

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	99.00	yes
125438	anaerobic	anaerobicⓘ	no	99.66	no
125438	spore-forming	spore-formingⓘ	no	87.57	yes
125438	thermophilic	thermophileⓘ	no	98.99	no
125438	aerobic	aerobicⓘ	yes	93.52	yes
125438	flagellated	motile2+ⓘ	yes	87.95	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Pseudomonas asiatica sp. nov., isolated from hospitalized patients in Japan and Myanmar.	Tohya M, Watanabe S, Teramoto K, Uechi K, Tada T, Kuwahara-Arai K, Kinjo T, Maeda S, Nakasone I, Zaw NN, Mya S, Zan KN, Tin HH, Fujita J, Kirikae T	Int J Syst Evol Microbiol	10.1099/ijsem.0.003316	2019

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#64384	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 107182
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#67868	Mari Tohya, Shin Watanabe, Kanae Teramoto, Kohei Uechi, Tatsuya Tada, Kyoko Kuwahara-Arai, Takeshi Kinjo, Shiro Maeda, Isamu Nakasone, Ni Ni Zaw, San Mya, Khin Nyein Zan, Htay Htay Tin, Jiro Fujita, Teruo Kirikae: Pseudomonas asiatica sp. nov., isolated from hospitalized patients in Japan and Myanmar. IJSEM 69: 1361 - 1368 2019 ( DOI 10.1099/ijsem.0.003316 )
#68369	Automatically annotated from API 20NE .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

Rate our new design

Content

About us

Pseudomonas asiatica (DSM 107182, JCM 32716, RYU-5)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Pigmentation

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Antibiotic susceptibility testing

Spore formation

Halophily

Observation

Metabolite utilization

Antibiotic resistance

Metabolite production

Metabolite tests

Enzymes

API 20NE

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Sequence information

Genome sequences

Genome browser: GCA_009932335

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

Help

Social Media