Name: Tuwongella immobilis MBLW1
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 157132

Type strain:

Species: Tuwongella immobilis

Strain Designation: MBLW1

Culture col. no.: CCUG 69661, DSM 105045

Strain history: <- B. Yee; Uppsala University, Uppsala, Sweden; MBLW1 <- M. Butler

NCBI tax ID(s): 692036 (species)

SI-ID 398427

DSM 105045, CCUG 69661

Special Collections

Section

When using BacDive for research please cite our paper

Tuwongella immobilis MBLW1 is an aerobe, chemoorganotroph, mesophilic bacterium that was isolated from Lake water.

Gram-negative
sphere-shaped
aerobe
chemoorganotroph
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	09-12-2024 (DD-MM-YYYY)
	Domain	*Bacteria*
	Phylum	*Planctomycetota*
	Class	*Planctomycetia*
	Order	*Gemmatales*
	Family	*Gemmataceae*
	Genus	*Tuwongella*
	Species	**Tuwongella immobilis**
	Full Scientific Name (LPSN)	Tuwongella immobilis Seeger et al. 2017

Information on morphological and physiological properties Morphology

[Ref.: #65238]	Gram stain	negative
[Ref.: #125438]	Gram stain	negative Confidence in %94

[Ref.: #65238]	Cell length	2.2-3.1 µm

[Ref.: #65238]	Cell shape	sphere-shaped

[Ref.: #65238]	Motility	no

[Ref.: #65238]	Colony color	pink

[Ref.: #63639]	Incubation period	7 days

Information on culture and growth conditions Culture and growth conditions

[Ref.: #64967]

Culture medium

ALKALIPHILES PIRELLULA MEDIUM (M1) (DSMZ Medium 1441)

[Ref.: #64967]

Culture medium growth

[Ref.: #64967]

Culture medium link

Medium recipe at MediaDive

[Ref.: #64967]

Culture medium composition

expand / minimize

[Ref.: #65238]

Culture medium

M1 agar

[Ref.: #65238]

Culture medium growth

[Ref.: #65238]

Culture medium

M1 liquid medium

[Ref.: #65238]

Culture medium growth

		Temperature
[Ref.: #63639]	growth	30 °C
[Ref.: #64967]	growth	28 °C
[Ref.: #65238]	growth	20-40 °C
[Ref.: #65238]	optimum	32-36 °C

	pH	Kind of pH		pH
[Ref.: #65238]			optimum	7.5-8
[Ref.: #65238]			growth	6-10.5

Information on physiology and metabolism Physiology and metabolism

[Ref.: #63639]	Oxygen tolerance	aerobe
[Ref.: #65238]	Oxygen tolerance	facultative aerobe
[Ref.: #125439]	Oxygen tolerance	obligate aerobe Confidence in %97.6

[Ref.: #65238]

Nutrition type

chemoorganotroph

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #65238]		NaCl		growth	0.4	%(w/v)

[Ref.: #65238]

Observation

Growth is inhibited by more than 50 % at NaCl concentrations greater than 0.4 % (w/v).

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #65238]	arabinose ChEBI	+	carbon source
[Ref.: #65238]	fructose ChEBI	-	assimilation
[Ref.: #65238]	galactose ChEBI	+	carbon source
[Ref.: #65238]	glucose ChEBI	+	carbon source
[Ref.: #65238]	glucose ChEBI	+	fermentation
[Ref.: #65238]	glycerol ChEBI	-	assimilation
[Ref.: #65238]	maltose ChEBI	+	carbon source
[Ref.: #65238]	mannitol ChEBI	-	assimilation
[Ref.: #65238]	N-acetylglucosamine ChEBI	+	carbon source
[Ref.: #65238]	N-acetylglucosamine ChEBI	+	fermentation
[Ref.: #65238]	rhamnose ChEBI	+	carbon source
[Ref.: #65238]	sucrose ChEBI	-	assimilation
[Ref.: #65238]	xylose ChEBI	-	assimilation
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Concentration		Antibiotic resistance	Concentration
[Ref.: #65238]	ampicillin ChEBI				yes	10	µg (disc)
[Ref.: #65238]	gentamicin ChEBI				yes	10	µg (disc)
[Ref.: #65238]	kanamycin ChEBI	yes	30	µg (disc)
[Ref.: #65238]	neomycin ChEBI	yes	10	µg (disc)
[Ref.: #65238]	streptomycin ChEBI				yes	10	µg (disc)

	Enzyme	Enzyme activity	EC number
[Ref.: #65238]	catalase	-	1.11.1.6
[Ref.: #65238]	cytochrome oxidase	+	1.9.3.1
[Ref.: #65238]	urease	+	3.5.1.5

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #63639]	Sample type/isolated from	Lake water
[Ref.: #63639]	Sampling date	2014-03-18
[Ref.: #63639]	Geographic location (country and/or sea, region)	University of Queensland
[Ref.: #63639]	Country	Australia
[Ref.: #63639]	Country ISO 3 Code	AUS
[Ref.: #63639]	Continent	Australia and Oceania

[Ref.: #64967]	Sample type/isolated from	lake
[Ref.: #64967]	Geographic location (country and/or sea, region)	University of Queensland, St. Lucia campus
[Ref.: #64967]	Country	Australia
[Ref.: #64967]	Country ISO 3 Code	AUS
[Ref.: #64967]	Continent	Australia and Oceania

[Ref.: #65238]	Sample type/isolated from	water sample from under the water surface at a level no deeper than 30 cm
[Ref.: #65238]	Geographic location (country and/or sea, region)	University freshwater Lake, on the St. Lucia campus of The University of Queensland, Brisbane, Queensland
[Ref.: #65238]	Country	Australia
[Ref.: #65238]	Country ISO 3 Code	AUS
[Ref.: #65238]	Continent	Australia and Oceania
[Ref.: #65238]	Enrichment culture	Lake Water (LW) agar plates
[Ref.: #65238]	Enrichment culture composition	15 g agar autoclaved in 250 ml distilled water, upon cooling 25 µg ml^-1 filter-sterilised ampicillin added and the volume made up to 1 l with the sterile filtrate that resulted above
[Ref.: #65238]	Enrichment culture duration	38 days
[Ref.: #65238]	Enrichment culture temperature	room temperature ̊C
[Ref.: #65238]	Isolation/enrichment procedure	Water samples were concentrated via filtration through a 0.45 µm membrane filter, with the concentrated particulate material on the filter being resuspended in approximately 3 ml of sterile lake water filtrate. Lake Water (LW) agar plates (15 g agar autoclaved in 250 ml distilled water, upon cooling 25 µg ml^-1 filter-sterilised ampicillin added and the volume made up to 1 l with the sterile filtrate that resulted above) were spread with 100 µl of the water concentrate. Plates were then incubate
* marker position based on {}

Isolation sources categories

#Environmental

#Aquatic

#Lake (large)

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence FJ811525 (>99% sequence identity) for Tuwongella immobilis subclade from Microbeatlas

Aquatic Samples	758
Soil Samples	155
Animal Samples	108
Plant Samples	28
Total Samples	1049

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #64967]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #64967]	Tuwongella immobilis strain MBLW1 16S ribosomal RNA gene, partial sequence	FJ811525	1455	GenBank ENA	692036 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Tuwongella immobilis MBLW1	GCA_901538355	complete	GenBank ENA	692036 tax ID	*
[Ref.: #66792]	Tuwongella immobilis MBLW1	2883404505	complete	JGI IMG/M	692036 tax ID	*

[Ref.: #64967]	GC-content	57 mol%	sequence analysis
[Ref.: #65238]	GC-content	57 mol%	genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Model	Trait	Prediction	Confidence in %	In training data
	Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Tuwongella immobilis MBLW1 NCBI: GCA_901538355.1
[Ref.: #125438]	gram-positive	gram-positiveⓘ	no	94	yes
[Ref.: #125438]	anaerobic	anaerobicⓘ	no	85.729	yes
[Ref.: #125438]	aerobic	aerobicⓘ	yes	72.633	no
[Ref.: #125438]	spore-forming	spore-formingⓘ	no	76.179	no
[Ref.: #125438]	thermophile	thermophilicⓘ	no	93.278	yes
[Ref.: #125438]	motile2+	flagellatedⓘ	no	78.911	yes

	Model	Trait	Prediction	Confidence in %
	Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Tuwongella immobilis MBLW1 IMG: 2883404505
[Ref.: #125439]	BacteriaNet	spore_formationⓘ	no	65.4
[Ref.: #125439]	BacteriaNet	motilityⓘ	yes	62.1
[Ref.: #125439]	BacteriaNet	gram_stainⓘ	negative	76.9
[Ref.: #125439]	BacteriaNet	oxygen_toleranceⓘ	obligate aerobe	97.6

Availability in culture collections External links

[Ref.: #64967]

Culture collection no.

CCUG 69661, DSM 105045

[Ref.: #110252]

SI-ID 398427

Literature:

Topic	Title	Authors	Journal	DOI	Year
Genetics	Complete genome sequence of the cellulolytic planctomycete Telmatocola sphagniphila SP2(T) and characterization of the first cellulolytic enzyme from planctomycetes.	Rakitin AL, Naumoff DG, Beletsky AV, Kulichevskaya IS, Mardanov AV, Ravin NV, Dedysh SN	Syst Appl Microbiol	10.1016/j.syapm.2021.126276	2021	*
Phylogeny	Tuwongella immobilis gen. nov., sp. nov., a novel non-motile bacterium within the phylum Planctomycetes.	Seeger C, Butler MK, Yee B, Mahajan M, Fuerst JA, Andersson SGE	Int J Syst Evol Microbiol	10.1099/ijsem.0.002271	2017	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#63639 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 69661

#64967

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 105045

#65238

Christian Seeger, Margaret K. Butler, Benjamin Yee, Mayank Mahajan, John A. Fuerst, Siv G. E. Andersson: Tuwongella immobilis gen. nov., sp. nov., a novel non-motile bacterium within the phylum Planctomycetes. IJSEM 67: 4923 - 4929 2017 ( DOI 10.1099/ijsem.0.002271 , PubMed 29087267 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#110252

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID398427.1 )

#125438

Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )

#125439

Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .

* These data were automatically processed and therefore are not curated

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