Name: Staphylococcus kloosii DSM 20676
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 14556

Type strain:

Species: Staphylococcus kloosii

Culture col. no.: DSM 20676, ATCC 43959, CCUG 30110, CCM 3834, SC 210, CIP 103503, GIFU 10764

Strain history: CIP <- 1995, N. El Solh, Inst. Pasteur, Paris, France <- K.H. Schleifer <- W.E. Kloos: strain SC 210

NCBI tax ID(s): 29384 (species)

SI-ID 105406

LMG 19130, ATCC 43959, DSM 20676, CCUG 30110, CCM 3834, CIP 103503, KCTC 3590, NCTC 12415, CECT 5328, NRRL B-14766, BCRC 13973, CCRC 13973

Other strains from Staphylococcus kloosii

S. kloosii GV 253, DSM 20677
S. kloosii FR2-36, DSM 30744
S. kloosii CCUG 38376, CCM 4532

Section

Staphylococcus kloosii DSM 20676 is an aerobe, mesophilic, Gram-positive bacterium that was isolated from skin of squirrel.

Gram-positive
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Staphylococcaceae*
	Genus	*Staphylococcus*
	Species	**Staphylococcus kloosii**
	Full Scientific Name (LPSN)	Staphylococcus kloosii Schleifer et al. 1985

Information on morphological and physiological properties Morphology

[Ref.: #120451]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #120451]	Cell shape	coccus-shaped

[Ref.: #120451]	Motility	no

[Ref.: #120451]

Hemolysis ability

Information on culture and growth conditions Culture and growth conditions

[Ref.: #8998]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #8998]

Culture medium growth

[Ref.: #8998]

Culture medium link

Medium recipe at MediaDive

[Ref.: #8998]

Culture medium composition

expand / minimize

[Ref.: #8998]

Culture medium

TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)

[Ref.: #8998]

Culture medium growth

[Ref.: #8998]

Culture medium link

Medium recipe at MediaDive

[Ref.: #8998]

Culture medium composition

expand / minimize

[Ref.: #37980]

Culture medium

MEDIUM 3 - Columbia agar

[Ref.: #37980]

Culture medium growth

[Ref.: #37980]

Culture medium composition

expand / minimize

[Ref.: #120451]

Culture medium

CIP Medium 3

[Ref.: #120451]

Culture medium growth

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Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #8998]		growth	37 °C
[Ref.: #37980]		growth	37 °C
[Ref.: #49913]		growth	37 °C
[Ref.: #120451]		growth	15-37 °C
[Ref.: #120451]	no	growth	45 °C

[Ref.: #8998]	Temperature range	mesophilic
[Ref.: #37980]	Temperature range	mesophilic
[Ref.: #49913]	Temperature range	mesophilic
[Ref.: #120451]	Temperature range	thermophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #49913]	Oxygen tolerance	aerobe
[Ref.: #120451]	Oxygen tolerance	obligate aerobe

[Ref.: #69480]

Ability of spore formation

no Confidence in %99.743

[Ref.: #8998]	Murein short key	A11.02
[Ref.: #8998]	Murein types	A3alpha L-Lys-Gly_5-6

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68371]	amygdalin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	arbutin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68375]	arginine ChEBI	-	hydrolysis	* from API ID32STA
[Ref.: #68371]	cellobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68375]	cellobiose ChEBI	-	fermentation	* from API ID32STA
[Ref.: #68371]	D-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fructose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68375]	D-fructose ChEBI	+	fermentation	* from API ID32STA
[Ref.: #68371]	D-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-galactose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-glucose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68375]	D-glucose ChEBI	+	fermentation	* from API ID32STA
[Ref.: #68371]	D-lyxose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannitol ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68375]	D-mannose ChEBI	-	fermentation	* from API ID32STA
[Ref.: #68371]	D-ribose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68375]	D-ribose ChEBI	+	fermentation	* from API ID32STA
[Ref.: #68371]	D-sorbitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-tagatose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #120451]	esculin ChEBI	-	hydrolysis
[Ref.: #68371]	esculin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68375]	esculin ChEBI	-	hydrolysis	* from API ID32STA
[Ref.: #68371]	galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gentiobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycerol ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycogen ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	inulin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabinose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-rhamnose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-sorbose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	lactose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	maltose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	melezitose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	melibiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-mannoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	myo-inositol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	N-acetylglucosamine ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68375]	N-acetylglucosamine ChEBI	-	fermentation	* from API ID32STA
[Ref.: #120451]	nitrate ChEBI	-	reduction
[Ref.: #68375]	nitrate ChEBI	-	reduction	* from API ID32STA
[Ref.: #120451]	nitrite ChEBI	-	reduction
[Ref.: #68375]	ornithine ChEBI	-	degradation	* from API ID32STA
[Ref.: #68371]	Potassium 2-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	Potassium 5-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	raffinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	salicin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	starch ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	sucrose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68375]	sucrose ChEBI	-	fermentation	* from API ID32STA
[Ref.: #68371]	trehalose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	turanose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68375]	turanose ChEBI	-	fermentation	* from API ID32STA
[Ref.: #68375]	urea ChEBI	+	hydrolysis	* from API ID32STA
[Ref.: #68371]	xylitol ChEBI	-	builds acid from	* from API 50CH acid
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #120451]		indole ChEBI	no
[Ref.: #68375]		acetoin ChEBI	no	* from API ID32STA

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #120451]		acetoin ChEBI	-
[Ref.: #68375]		acetoin ChEBI	-	* from API ID32STA

	Enzyme	Enzyme activity	EC number
[Ref.: #120451]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68375]	alkaline phosphatase	+	3.1.3.1	* from API ID32STA
[Ref.: #68375]	arginine dihydrolase	-	3.5.3.6	* from API ID32STA
[Ref.: #120451]	beta-galactosidase	+	3.2.1.23
[Ref.: #68375]	beta-galactosidase	+	3.2.1.23	* from API ID32STA
[Ref.: #68375]	beta-glucosidase	-	3.2.1.21	* from API ID32STA
[Ref.: #68375]	beta-glucuronidase	+	3.2.1.31	* from API ID32STA
[Ref.: #120451]	caseinase	+	3.4.21.50
[Ref.: #120451]	catalase	+	1.11.1.6
[Ref.: #120451]	coagulase	-
[Ref.: #120451]	DNase	-
[Ref.: #120451]	gelatinase	-
[Ref.: #68375]	L-arginine arylamidase	-		* from API ID32STA
[Ref.: #120451]	lecithinase	-
[Ref.: #120451]	lysine decarboxylase	-	4.1.1.18
[Ref.: #120451]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #68375]	ornithine decarboxylase	-	4.1.1.17	* from API ID32STA
[Ref.: #120451]	oxidase	-
[Ref.: #120451]	urease	+	3.5.1.5
[Ref.: #68375]	urease	+	3.5.1.5	* from API ID32STA
	Only first 10 entries are displayed. Click here to see all.

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #120451]	9290	not determinedn.d.	+	-	-	+	+	-	-	-	-	+	+	+	+	-	-	-	-	+	-	-	-	-	-	-	-	-	-	+	+	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

API® ID32STA:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Urease/urea hydrolysisURE	Arginine dihydrolaseADH (Arg)	Ornithine decarboxylaseODC	Esculin hydrolysisESC	Fermentation of D-glucoseGLU	Fermentation of D-fructoseFRU	Fermentation of D-mannoseMNE	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of trehaloseTRE	Fermentation of D-mannitolMAN	Fermentation of raffinoseRAF	Fermentation of D-riboseRIB	Fermentation of cellobioseCEL	Reduction of nitrateNIT	Acetoin production (Voges Proskauer test)VP	beta-Galactosidasebeta GAL	L-arginine arylamidaseArgA	Alkaline phosphatasePAL	Pyrrolidonyl arylamidasePyrA	Novobiocin resistanceNOVO	Fermentation of sucroseSAC	Fermentation of N-acetyl-glucosamineNAG	Fermentation of turanoseTUR	Fermentation of arabinoseARA	beta-Glucuronidasebeta GUR
[Ref.: #8998]	6077	+	-	-	-	+	+	-	+	+	+	+	-	+	-	-	-	+	-	+	+	+	-	-	-	+	+
[Ref.: #8998]	6076	+	-	-	-	+	+	-	+/-	+/-	+/-	+/-	+	+	-	not determinedn.d.	-	+	-	+	+/-	+/-	-	-	-	+/-	+

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #8998]	Sample type/isolated from	skin of squirrel

[Ref.: #49913]	Sample type/isolated from	Squirrel skin
[Ref.: #49913]	Country	USA
[Ref.: #49913]	Country ISO 3 Code	USA
[Ref.: #49913]	Continent	North America

[Ref.: #120451]	Sample type/isolated from	Animal, Squirrel, skin
* marker position based on {}

Isolation sources categories

#Host	#Mammals	#Rodentia (Other)
#Host Body-Site	#Organ	#Skin, Nail, Hair

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AB009940 (>99% sequence identity) for Staphylococcus from Microbeatlas

Aquatic Samples	8
Soil Samples	7
Animal Samples	454
Plant Samples	12
Total Samples	481

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #8998]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #120451]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #8998]	Staphylococcus kloosii rrn gene for 16S ribosomal RNA	AB009940	1494	ENA	29384 tax ID
[Ref.: #20218]	Staphylococcus kloosii strain DSM 20676 16S ribosomal RNA gene, partial sequence	AY688071	464	ENA	29384 tax ID	*
[Ref.: #20218]	Staphylococcus kloosii 16S ribosomal RNA gene, partial sequence	AF041360	913	ENA	29384 tax ID	*
[Ref.: #20218]	S.arlettae gene for 16S ribosomal RNA	Z26888	771	ENA	29378 tax ID	*
[Ref.: #20218]	S.kloosii gene for 16S ribosomal RNA	Z26898	771	ENA	29384 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Staphylococcus kloosii ATCC 43959	GCA_003019255	complete	GenBank	29384 tax ID	*
[Ref.: #66792]	Staphylococcus kloosii strain ATCC 43959	29384.15	complete	PATRIC	29384 tax ID	*

[Ref.: #8998]

GC-content

32.3 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Staphylococcus kloosii strain ATCC 43959 PATRIC: 29384.15
[Ref.: #69481]	spore-forming	no	89	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Staphylococcus kloosii ATCC 43959 NCBI: GCA_003019255.1
[Ref.: #69480]	flagellated	no	89.593	no
[Ref.: #69480]	gram-positive	yes	89.042	no
[Ref.: #69480]	anaerobic	no	99.326	yes
[Ref.: #69480]	halophile	yes	95.339	no
[Ref.: #69480]	spore-forming	no	88.846	no
[Ref.: #69480]	glucose-util	yes	89.1	no
[Ref.: #69480]	aerobic	yes	91.246	no
[Ref.: #69480]	thermophile	no	99.826	yes
[Ref.: #69480]	motile	no	87.576	no
[Ref.: #69480]	glucose-ferment	yes	81.708	yes

Availability in culture collections External links

[Ref.: #8998]

Culture collection no.

DSM 20676, ATCC 43959, CCUG 30110, CCM 3834, SC 210, CIP 103503, GIFU 10764

[Ref.: #83687]

SI-ID 105406

Literature:

Topic	Title	Authors	Journal	DOI	Year
Genetics	Complete Genome Sequences of the Potential Zoonotic Pathogens Staphylococcus felis and Staphylococcus kloosii.	Mascarenhas Dos Santos AC, Jie R, Antunes Godoy H, Alves M, Pombert JF	Genome Announc	10.1128/genomeA.00404-18	2018	*

References

#8998

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 20676

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#37980 ; Curators of the CIP;
#49913 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 30110

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68371

Automatically annotated from API 50CH acid .

#68375

Automatically annotated from API ID32STA .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#83687

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID105406.1 )

#120451 Collection of Institut Pasteur ; Curators of the CIP; CIP 103503
* These data were automatically processed and therefore are not curated

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Staphylococcus kloosii strain ATCC 43959

Staphylococcus kloosii ATCC 43959

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Change proposal