Staphylococcus kloosii (DSM 20676, ATCC 43959, CCUG 30110)

Name: Staphylococcus kloosii (DSM 20676, ATCC 43959, CCUG 30110)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 14556

Type strain

Culture col. no. DSM 20676 ATCC 43959 CCUG 30110 CCM 3834 SC 210 2 more

NCBI tax ID(s) 29384

Special Collections DSMZ CCUG CIP

Links

Staphylococcus kloosii DSM 20676 is an aerobe, mesophilic, Gram-positive prokaryote that was isolated from skin of squirrel.

Gram-positive coccus-shaped aerobe mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 105406

LMG 19130,ATCC 43959,DSM 20676,PCM 2440,CCUG 30110,CCM 3834,CIP 103503,KCTC 3590,NCTC 12415,CECT 5328,NRRL B-14766,BCRC 13973,CCRC 13973

@ref 20215

Last LPSN update 2025-12-16 09:50:23

Domain Bacillati

Phylum Bacillota

Class Bacilli

Order Caryophanales

Family Staphylococcaceae

Genus Staphylococcus

Species Staphylococcus kloosii

Full scientific name Staphylococcus kloosii Schleifer et al. 1985

BacDive ID	Other strains from **Staphylococcus kloosii** (3)	Type strain
14557	S. kloosii GV 253, DSM 20677
14558	S. kloosii FR2-36, DSM 30744
149773	S. kloosii CCUG 38376, CCM 4532

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility
120451	positive	coccus-shaped

Colony morphology

120451

Hemolysis ability1

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
8998	COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)	Medium recipe at MediaDive	Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
8998	TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)	Medium recipe at MediaDive	Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
37980	MEDIUM 3 - Columbia agar		Columbia agar (39.000 g);distilled water (1000.000 ml)
120451	CIP Medium 3	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
8998	positive	growth	37	mesophilic
37980	positive	growth	37	mesophilic
49913	positive	growth	37	mesophilic
120451	positive	growth	15-37
120451	negative	growth	45

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance
49913	aerobe
120451	obligate aerobe
125439	obligate aerobe

Murein

@ref	Murein short key	Type
8998	A11.02	A3alpha L-Lys-Gly_5-6

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68371	27613 ChEBI	amygdalin	-	builds acid from	from API 50CH acid
68371	18305 ChEBI	arbutin	-	builds acid from	from API 50CH acid
68375	29016 ChEBI	arginine	-	hydrolysis	from API ID32STA
68375	17057 ChEBI	cellobiose	-	fermentation	from API ID32STA
68371	17057 ChEBI	cellobiose	-	builds acid from	from API 50CH acid
68371	17108 ChEBI	D-arabinose	-	builds acid from	from API 50CH acid
68371	18333 ChEBI	D-arabitol	-	builds acid from	from API 50CH acid
68375	15824 ChEBI	D-fructose	+	fermentation	from API ID32STA
68371	15824 ChEBI	D-fructose	+	builds acid from	from API 50CH acid
68371	28847 ChEBI	D-fucose	-	builds acid from	from API 50CH acid
68371	12936 ChEBI	D-galactose	+	builds acid from	from API 50CH acid
68375	17634 ChEBI	D-glucose	+	fermentation	from API ID32STA
68371	17634 ChEBI	D-glucose	+	builds acid from	from API 50CH acid
68371	62318 ChEBI	D-lyxose	-	builds acid from	from API 50CH acid
68371	16899 ChEBI	D-mannitol	+	builds acid from	from API 50CH acid
68375	16024 ChEBI	D-mannose	-	fermentation	from API ID32STA
68371	16024 ChEBI	D-mannose	+	builds acid from	from API 50CH acid
68375	16988 ChEBI	D-ribose	+	fermentation	from API ID32STA
68371	16988 ChEBI	D-ribose	+	builds acid from	from API 50CH acid
68371	17924 ChEBI	D-sorbitol	-	builds acid from	from API 50CH acid
68371	16443 ChEBI	D-tagatose	-	builds acid from	from API 50CH acid
68371	65327 ChEBI	D-xylose	-	builds acid from	from API 50CH acid
68371	17113 ChEBI	erythritol	-	builds acid from	from API 50CH acid
68375	4853 ChEBI	esculin	-	hydrolysis	from API ID32STA
120451	4853 ChEBI	esculin	-	hydrolysis
68371	4853 ChEBI	esculin	-	builds acid from	from API 50CH acid
68371	16813 ChEBI	galactitol	-	builds acid from	from API 50CH acid
68371	28066 ChEBI	gentiobiose	-	builds acid from	from API 50CH acid
68371	24265 ChEBI	gluconate	-	builds acid from	from API 50CH acid
68371	17754 ChEBI	glycerol	+	builds acid from	from API 50CH acid
68371	28087 ChEBI	glycogen	-	builds acid from	from API 50CH acid
68371	15443 ChEBI	inulin	-	builds acid from	from API 50CH acid
68371	30849 ChEBI	L-arabinose	+	builds acid from	from API 50CH acid
68371	18403 ChEBI	L-arabitol	-	builds acid from	from API 50CH acid
68371	18287 ChEBI	L-fucose	-	builds acid from	from API 50CH acid
68371	62345 ChEBI	L-rhamnose	-	builds acid from	from API 50CH acid
68371	17266 ChEBI	L-sorbose	-	builds acid from	from API 50CH acid
68371	65328 ChEBI	L-xylose	-	builds acid from	from API 50CH acid
68371	17716 ChEBI	lactose	+	builds acid from	from API 50CH acid
68371	17306 ChEBI	maltose	+	builds acid from	from API 50CH acid
68371	6731 ChEBI	melezitose	-	builds acid from	from API 50CH acid
68371	28053 ChEBI	melibiose	-	builds acid from	from API 50CH acid
68371	320061 ChEBI	methyl alpha-D-glucopyranoside	-	builds acid from	from API 50CH acid
68371	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from	from API 50CH acid
68371	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from	from API 50CH acid
68371	17268 ChEBI	myo-inositol	-	builds acid from	from API 50CH acid
68375	59640 ChEBI	N-acetylglucosamine	-	fermentation	from API ID32STA
68371	59640 ChEBI	N-acetylglucosamine	-	builds acid from	from API 50CH acid
68375	17632 ChEBI	nitrate	-	reduction	from API ID32STA
120451	17632 ChEBI	nitrate	-	reduction
120451	16301 ChEBI	nitrite	-	reduction
68375	18257 ChEBI	ornithine	-	degradation	from API ID32STA
68371		Potassium 2-ketogluconate	-	builds acid from	from API 50CH acid
68371		Potassium 5-ketogluconate	-	builds acid from	from API 50CH acid
68371	16634 ChEBI	raffinose	-	builds acid from	from API 50CH acid
68371	15963 ChEBI	ribitol	-	builds acid from	from API 50CH acid
68371	17814 ChEBI	salicin	-	builds acid from	from API 50CH acid
68371	28017 ChEBI	starch	-	builds acid from	from API 50CH acid
68375	17992 ChEBI	sucrose	-	fermentation	from API ID32STA
68371	17992 ChEBI	sucrose	-	builds acid from	from API 50CH acid
68371	27082 ChEBI	trehalose	+	builds acid from	from API 50CH acid
68375	32528 ChEBI	turanose	-	fermentation	from API ID32STA
68371	32528 ChEBI	turanose	-	builds acid from	from API 50CH acid
68375	16199 ChEBI	urea	+	hydrolysis	from API ID32STA
68371	17151 ChEBI	xylitol	-	builds acid from	from API 50CH acid

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68375	15688 ChEBI	acetoin		from API ID32STA
120451	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test
68375	15688 ChEBI	acetoin	-	from API ID32STA
120451	15688 ChEBI	acetoin	-

Enzymes

@ref	Value	Activity	Ec
120451	alcohol dehydrogenase	-	1.1.1.1
68375	alkaline phosphatase	+	3.1.3.1	from API ID32STA
68375	arginine dihydrolase	-	3.5.3.6	from API ID32STA
120451	beta-galactosidase	+	3.2.1.23
68375	beta-galactosidase	+	3.2.1.23	from API ID32STA
68375	beta-glucosidase	-	3.2.1.21	from API ID32STA
68375	beta-glucuronidase	+	3.2.1.31	from API ID32STA
120451	caseinase	+	3.4.21.50
120451	catalase	+	1.11.1.6
120451	coagulase	-
120451	DNase	-
120451	gelatinase	-
68375	L-arginine arylamidase	-		from API ID32STA
120451	lecithinase	-
120451	lysine decarboxylase	-	4.1.1.18
120451	ornithine decarboxylase	-	4.1.1.17
68375	ornithine decarboxylase	-	4.1.1.17	from API ID32STA
120451	oxidase	-
120451	urease	+	3.5.1.5
68375	urease	+	3.5.1.5	from API ID32STA

API 50CHac

@ref	ControlQ	GLY	ERY	DARA	LARA	RIB	DXYL	LXYL	ADO	MDX	GAL	GLU	FRU	MNE	SBE	RHA	DUL	INO	MAN	SOR	MDM	MDG	NAG	AMY	ARB	ESC	SAL	CEL	MAL	LAC	MEL	SAC	TRE	INU	MLZ	RAF	AMD	GLYG	XLT	GEN	TUR	LYX	TAG	DFUC	LFUC	DARL	LARL	GNT	2KG	5KG
120451	not determinedn.d.	+	-	-	+	+	-	-	-	-	+	+	+	+	-	-	-	-	+	-	-	-	-	-	-	-	-	-	+	+	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

API ID32STA

@ref	URE	ADH (Arg)	ODC	ESC	GLU	FRU	MNE	MAL	LAC	TRE	MAN	RAF	RIB	CEL	Reduction of nitrateNIT	Acetoin production (Voges Proskauer test)VP	beta GAL	L-arginine arylamidaseArgA	PAL	Pyrrolidonyl arylamidasePyrA	NOVO	SAC	NAG	TUR	ARA	beta GUR
8998	+	-	-	-	+	+	-	+	+	+	+	-	+	-	-	-	+	-	+	+	+	-	-	-	+	+
8998	+	-	-	-	+	+	-	+/-	+/-	+/-	+/-	+	+	-	not determinedn.d.	-	+	-	+	+/-	+/-	-	-	-	+/-	+

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host	#Mammals	#Rodentia (Other)
#Host Body-Site	#Organ	#Skin, Nail, Hair

Isolation

@ref	Sample type
8998	skin of squirrel
49913	Squirrel skin
120451	Animal, Squirrel, skin

Taxonmaps

69479

Global distribution of 16S sequence AB009940 (>99% sequence identity) for Staphylococcus from Microbeatlas

Aquatic Samples	8
Soil Samples	7
Animal Samples	454
Plant Samples	12
Total Samples	481

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
8998	1	Risk group (German classification) According to TRBA 466
120451	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM301925v1 assembly for Staphylococcus kloosii ATCC 43959	complete	GCA_003019255	29384.15	2969589784	29384	97.45

Genome browser: GCA_003019255

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Staphylococcus kloosii 16S ribosomal RNA gene, partial sequence	AF041360	913	29384
20218	Staphylococcus kloosii strain DSM 20676 16S ribosomal RNA gene, partial sequence	AY688071	464	29384
20218	S.arlettae gene for 16S ribosomal RNA	Z26888	771	29378
20218	S.kloosii gene for 16S ribosomal RNA	Z26898	771	29384
8998	Staphylococcus kloosii rrn gene for 16S ribosomal RNA	AB009940	1494	29384
124043	Staphylococcus kloosii strain ATCC 43959 16S ribosomal RNA gene, partial sequence.	ON041093	1411	29384

GC content

8998

GC-content (mol%)32.3

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Staphylococcus kloosii strain ATCC 43959
PATRIC: 29384.15

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	81.20	no
125439	motility	BacteriaNetⓘ	no	59.30	no
125439	gram_stain	BacteriaNetⓘ	positive	75.20	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	93.40	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Staphylococcus kloosii ATCC 43959
NCBI: GCA_003019255.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	80.29	no
125438	anaerobic	anaerobicⓘ	no	99.17	yes
125438	aerobic	aerobicⓘ	yes	74.40	yes
125438	spore-forming	spore-formingⓘ	no	72.41	no
125438	thermophilic	thermophileⓘ	no	94.67	yes
125438	flagellated	motile2+ⓘ	no	81.33	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Enzymology	Colonization patterns of Enterococcus cecorum in two different broiler production cycles detected with a newly developed quantitative real-time PCR.	Jung A, Petersen H, Teske L, Rautenschlein S.	BMC Microbiol	10.1186/s12866-017-1021-7	2017
Phylogeny	Comparative study using various methods for identification of Staphylococcus species in clinical specimens.	Layer F, Ghebremedhin B, Moder KA, Konig W, Konig B.	J Clin Microbiol	10.1128/jcm.00226-06	2006
	Bacterial contaminants from frozen puff pastry production process and their growth inhibition by antimicrobial substances from lactic acid bacteria.	Rumjuankiat K, Keawsompong S, Nitisinprasert S.	Food Sci Nutr	10.1002/fsn3.413	2017
Phylogeny	Identification of Staphylococcus spp. by PCR-restriction fragment length polymorphism of gap gene.	Yugueros J, Temprano A, Sanchez M, Luengo JM, Naharro G.	J Clin Microbiol	10.1128/jcm.39.10.3693-3695.2001	2001
	Mobilizable antibiotic resistance genes are present in dust microbial communities.	Ben Maamar S, Glawe AJ, Brown TK, Hellgeth N, Hu J, Wang JP, Huttenhower C, Hartmann EM.	PLoS Pathog	10.1371/journal.ppat.1008211	2020
Phylogeny	Rapid and accurate species-level identification of coagulase-negative staphylococci by using the sodA gene as a target.	Poyart C, Quesne G, Boumaila C, Trieu-Cuot P.	J Clin Microbiol	10.1128/jcm.39.12.4296-4301.2001	2001
Phylogeny	Identification of Staphylococcus species and subspecies by the chaperonin 60 gene identification method and reverse checkerboard hybridization.	Goh SH, Santucci Z, Kloos WE, Faltyn M, George CG, Driedger D, Hemmingsen SM.	J Clin Microbiol	10.1128/jcm.35.12.3116-3121.1997	1997
Genetics	Complete Genome Sequences of the Potential Zoonotic Pathogens Staphylococcus felis and Staphylococcus kloosii.	Mascarenhas Dos Santos AC, Jie R, Antunes Godoy H, Alves M, Pombert JF	Genome Announc	10.1128/genomeA.00404-18	2018

References

#8998	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 20676
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#37980	; Curators of the CIP;
#49913	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 30110
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#68371	Automatically annotated from API 50CH acid .
#68375	Automatically annotated from API ID32STA .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#120451	Collection of Institut Pasteur ; Curators of the CIP; CIP 103503
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Staphylococcus kloosii (DSM 20676, ATCC 43959, CCUG 30110)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Murein

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API 50CHac

API ID32STA

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_003019255

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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