Name: Spiroplasma alleghenense PLHS-1
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 14377

Type strain:

Species: Spiroplasma alleghenense

Strain Designation: PLHS-1

Culture col. no.: DSM 19900, ATCC 51752

Strain history: <- J. K. Davis {2007} <- E. A. Clark and R. F. Whitcomb

NCBI tax ID(s): 216931 (species)

SI-ID 43161

ATCC 51752

Section

Spiroplasma alleghenense PLHS-1 is a microaerophile, mesophilic, Gram-positive bacterium that was isolated from hemolyph of scorpion fly .

Gram-positive
mesophilic
microaerophile
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Mycoplasmatota*
	Class	*Mollicutes*
	Order	*Mycoplasmatales*
	Family	*Spiroplasmataceae*
	Genus	*Spiroplasma*
	Species	**Spiroplasma alleghenense**
	Full Scientific Name (LPSN)	Spiroplasma alleghenense Adams et al. 1997

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	positive Confidence in %98.914

Information on culture and growth conditions Culture and growth conditions

[Ref.: #8374]

Culture medium

SP4-Z MEDIUM (DSMZ Medium 1076b)

[Ref.: #8374]

Culture medium growth

[Ref.: #8374]

Culture medium link

Medium recipe at MediaDive

[Ref.: #8374]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #8374]			growth	30 °C

[Ref.: #8374]

Temperature range

mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #8374]

Oxygen tolerance

microaerophile

[Ref.: #69480]

Ability of spore formation

no Confidence in %99.999

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #8374]	Sample type/isolated from	hemolyph of scorpion fly ( Panorpa helena)
[Ref.: #8374]	Host species	Panorpa helena
[Ref.: #8374]	Country	USA
[Ref.: #8374]	Country ISO 3 Code	USA
[Ref.: #8374]	Continent	North America
* marker position based on {}

Isolation sources categories

#Host	#Arthropoda	#Insecta
#Host Body Product	#Fluids	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AY189125 (>99% sequence identity) for Spiroplasma from Microbeatlas

Aquatic Samples	1
Soil Samples	2
Animal Samples	22
Plant Samples
Total Samples	25

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #8374]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Spiroplasma alleghenense 16S ribosomal RNA gene, partial sequence	AY189125	1516	ENA	216931 tax ID	*
[Ref.: #20218]	Spiroplasma alleghenense strain PLHS-1 16S ribosomal RNA gene, partial sequence; 16S-23S intergenic spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence	DQ004922	912	ENA	216931 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Spiroplasma alleghenense PLHS-1	GCA_003363775	complete	GenBank	216931 tax ID	*
[Ref.: #66792]	Spiroplasma alleghenense strain PLHS-1	216931.3	complete	PATRIC	216931 tax ID	*
[Ref.: #66792]	Spiroplasma alleghenense PLHS-1	2843673047	complete	JGI IMG/M	1336750 tax ID	*

[Ref.: #8374]

GC-content

31 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Spiroplasma alleghenense PLHS-1 NCBI: GCA_003363775.1
[Ref.: #69481]	spore-forming	no	75	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Spiroplasma alleghenense PLHS-1 NCBI: GCA_003363775.1
[Ref.: #69480]	motile	no	91.675	no
[Ref.: #69480]	flagellated	no	98.162	no
[Ref.: #69480]	gram-positive	yes	65.528	no
[Ref.: #69480]	anaerobic	no	94.057	yes
[Ref.: #69480]	aerobic	no	97.954	yes
[Ref.: #69480]	halophile	no	52.288	no
[Ref.: #69480]	spore-forming	no	97.996	no
[Ref.: #69480]	glucose-util	yes	92.318	no
[Ref.: #69480]	thermophile	no	88.736	no
[Ref.: #69480]	glucose-ferment	yes	80.365	no

Availability in culture collections External links

[Ref.: #8374]

Culture collection no.

DSM 19900, ATCC 51752

[Ref.: #83520]

SI-ID 43161

Literature:

Topic	Title	Authors	Journal	DOI	Year
Genetics	Complete Genome Sequence of Spiroplasma alleghenense PLHS-1(T) (ATCC 51752), a Bacterium Isolated from Scorpion Fly (Panorpa helena).	Chou L, Lee TY, Tsai YM, Kuo CH	Microbiol Resour Announc	10.1128/MRA.00317-19	2019	*
Phylogeny	Revised group classification of the genus Spiroplasma.	Williamson DL, Whitcomb RF, Tully JG, Gasparich GE, Rose DL, Carle P, Bove JM, Hackett KJ, Adams JR, Henegar RB, Konai M, Chastel C, French FE	Int J Syst Bacteriol	10.1099/00207713-48-1-1	1998	*

References

#8374

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 19900

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#83520

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID43161.1 )

* These data were automatically processed and therefore are not curated

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