Name: Spiroplasma litorale TN-1
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 14371

Type strain:

Species: Spiroplasma litorale

Strain Designation: TN-1

Culture col. no.: DSM 21781, ATCC 43211

Strain history: <- J. K. Davis {2008} <- J. G. Tully <- T. B. Clark, USDA, Beltsville

NCBI tax ID(s): 216942 (species)

SI-ID 45117

ATCC 43211

Section

Spiroplasma litorale TN-1 is a microaerophile, mesophilic bacterium that was isolated from gut of horsefly, Tabanus nigrovittatus.

mesophilic
microaerophile
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Mycoplasmatota*
	Class	*Mollicutes*
	Order	*Mycoplasmatales*
	Family	*Spiroplasmataceae*
	Genus	*Spiroplasma*
	Species	**Spiroplasma litorale**
	Full Scientific Name (LPSN)	Spiroplasma litorale Konai et al. 1997

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	positive Confidence in %99.719

[Ref.: #69480]	Motility	no Confidence in %91.546

Information on culture and growth conditions Culture and growth conditions

[Ref.: #15946]

Culture medium

SP4-Z MEDIUM (DSMZ Medium 1076b)

[Ref.: #15946]

Culture medium growth

[Ref.: #15946]

Culture medium link

Medium recipe at MediaDive

[Ref.: #15946]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #15946]			growth	30 °C

[Ref.: #15946]

Temperature range

mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #15946]

Oxygen tolerance

microaerophile

[Ref.: #69480]

Ability of spore formation

no Confidence in %99.999

	Pathway	Enzyme coverage	Annotated reactions
	Pathway annotation	Computationally determined by
[Ref.: #66794]	L-lactaldehyde degradation	100	3 of 3
[Ref.: #66794]	coenzyme A metabolism	100	4 of 4
[Ref.: #66794]	formaldehyde oxidation	100	3 of 3
[Ref.: #66794]	CDP-diacylglycerol biosynthesis	100	2 of 2
[Ref.: #66794]	folate polyglutamylation	100	1 of 1
[Ref.: #66794]	palmitate biosynthesis	77.27	17 of 22
[Ref.: #66794]	ppGpp biosynthesis	75	3 of 4
[Ref.: #66794]	photosynthesis	71.43	10 of 14
[Ref.: #66794]	glycolysis	70.59	12 of 17
[Ref.: #66794]	degradation of sugar alcohols	68.75	11 of 16
[Ref.: #66794]	aspartate and asparagine metabolism	66.67	6 of 9
[Ref.: #66794]	acetoin degradation	66.67	2 of 3
[Ref.: #66794]	UDP-GlcNAc biosynthesis	66.67	2 of 3
[Ref.: #66794]	purine metabolism	60.64	57 of 94
[Ref.: #66794]	pyrimidine metabolism	60	27 of 45
[Ref.: #66794]	NAD metabolism	55.56	10 of 18
[Ref.: #66794]	pentose phosphate pathway	54.55	6 of 11
[Ref.: #66794]	butanoate fermentation	50	2 of 4
[Ref.: #66794]	ketogluconate metabolism	50	4 of 8
[Ref.: #66794]	acetate fermentation	50	2 of 4
[Ref.: #66794]	ethanol fermentation	50	1 of 2
[Ref.: #66794]	sulfopterin metabolism	50	2 of 4
[Ref.: #66794]	cis-vaccenate biosynthesis	50	1 of 2
[Ref.: #66794]	adipate degradation	50	1 of 2
[Ref.: #66794]	oxidative phosphorylation	49.45	45 of 91
[Ref.: #66794]	d-xylose degradation	45.45	5 of 11
[Ref.: #66794]	metabolism of disaccharids	45.45	5 of 11
[Ref.: #66794]	d-mannose degradation	44.44	4 of 9
[Ref.: #66794]	serine metabolism	44.44	4 of 9
[Ref.: #66794]	propanol degradation	42.86	3 of 7
[Ref.: #66794]	lipoate biosynthesis	40	2 of 5
[Ref.: #66794]	cellulose degradation	40	2 of 5
[Ref.: #66794]	metabolism of amino sugars and derivatives	40	2 of 5
[Ref.: #66794]	methylglyoxal degradation	40	2 of 5
[Ref.: #66794]	vitamin B1 metabolism	38.46	5 of 13
[Ref.: #66794]	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	37.5	3 of 8
[Ref.: #66794]	isoprenoid biosynthesis	34.62	9 of 26
[Ref.: #66794]	acetyl CoA biosynthesis	33.33	1 of 3
[Ref.: #66794]	octane oxidation	33.33	1 of 3
[Ref.: #66794]	selenocysteine biosynthesis	33.33	2 of 6
[Ref.: #66794]	IAA biosynthesis	33.33	1 of 3
[Ref.: #66794]	lipid metabolism	32.26	10 of 31
[Ref.: #66794]	degradation of pentoses	32.14	9 of 28
[Ref.: #66794]	non-pathway related	31.58	12 of 38
[Ref.: #66794]	methionine metabolism	30.77	8 of 26
[Ref.: #66794]	Entner Doudoroff pathway	30	3 of 10
[Ref.: #66794]	threonine metabolism	30	3 of 10
[Ref.: #66794]	reductive acetyl coenzyme A pathway	28.57	2 of 7
[Ref.: #66794]	glutamate and glutamine metabolism	28.57	8 of 28
[Ref.: #66794]	tetrahydrofolate metabolism	28.57	4 of 14
[Ref.: #66794]	flavin biosynthesis	26.67	4 of 15
[Ref.: #66794]	androgen and estrogen metabolism	25	4 of 16
[Ref.: #66794]	cyclohexanol degradation	25	1 of 4
[Ref.: #66794]	lactate fermentation	25	1 of 4
[Ref.: #66794]	glycogen biosynthesis	25	1 of 4
[Ref.: #66794]	degradation of aromatic, nitrogen containing compounds	25	3 of 12
[Ref.: #66794]	tryptophan metabolism	23.68	9 of 38
[Ref.: #66794]	phenylalanine metabolism	23.08	3 of 13
[Ref.: #66794]	valine metabolism	22.22	2 of 9
[Ref.: #66794]	CO2 fixation in Crenarchaeota	22.22	2 of 9
[Ref.: #66794]	lipid A biosynthesis	22.22	2 of 9
[Ref.: #66794]	nitrate assimilation	22.22	2 of 9
[Ref.: #66794]	glutathione metabolism	21.43	3 of 14
		Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #15946]	Sample type/isolated from	gut of horsefly, Tabanus nigrovittatus
[Ref.: #15946]	Host species	Tabanus nigrovittatus
[Ref.: #15946]	Geographic location (country and/or sea, region)	North Carolina, Ocracoke
[Ref.: #15946]	Country	USA
[Ref.: #15946]	Country ISO 3 Code	USA
[Ref.: #15946]	Continent	North America
* marker position based on {}

Isolation sources categories

#Host	#Arthropoda	#Insecta
#Host Body-Site	#Gastrointestinal tract	#Stomach

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AY189306 (>99% sequence identity) for Spiroplasma litorale from Microbeatlas

Aquatic Samples	3
Soil Samples	1
Animal Samples	111
Plant Samples
Total Samples	115

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #15946]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #15946]	Spiroplasma litorale 16S ribosomal RNA gene, partial sequence	AY189306	1516	ENA	216942 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Spiroplasma litorale TN-1	GCA_001267155	complete	GenBank	216942 tax ID	*
[Ref.: #66792]	Spiroplasma litorale strain TN-1	216942.3	complete	PATRIC	216942 tax ID	*
[Ref.: #66792]	Spiroplasma litorale TN-1	2636415681	complete	JGI IMG/M	216942 tax ID	*

[Ref.: #15946]

GC-content

25 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Spiroplasma litorale TN-1 NCBI: GCA_001267155.1
[Ref.: #69481]	spore-forming	no	80	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Spiroplasma litorale TN-1 NCBI: GCA_001267155.1
[Ref.: #69480]	motile	no	92.584	no
[Ref.: #69480]	flagellated	no	98.083	no
[Ref.: #69480]	gram-positive	yes	75.566	no
[Ref.: #69480]	anaerobic	no	93.851	no
[Ref.: #69480]	aerobic	no	98.487	yes
[Ref.: #69480]	halophile	yes	53.037	no
[Ref.: #69480]	spore-forming	no	97.832	no
[Ref.: #69480]	thermophile	no	95.287	no
[Ref.: #69480]	glucose-util	yes	88.471	no
[Ref.: #69480]	glucose-ferment	yes	76.632	no

Availability in culture collections External links

[Ref.: #15946]

Culture collection no.

DSM 21781, ATCC 43211

[Ref.: #83514]

SI-ID 45117

Literature:

Topic	Title	Authors	Journal	DOI	Year
Genetics	Complete Genome Sequence of Spiroplasma litorale TN-1T (DSM 21781), a Bacterium Isolated from a Green-Eyed Horsefly (Tabanus nigrovittatus).	Lo WS, Lai YC, Lien YW, Wang TH, Kuo CH	Genome Announc	10.1128/genomeA.01116-15	2015	*

References

#15946

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 21781

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#66794

Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#83514

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID45117.1 )

* These data were automatically processed and therefore are not curated

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