Bacillus tropicus N24 | Type strain | MCCC 1A01406, KCTC 33711, LMG 28874

Strain identifier

BacDive ID: 140962

Type strain:

Species: Bacillus tropicus

Strain Designation: N24

Culture col. no.: MCCC 1A01406, KCTC 33711, LMG 28874

Section

Name and taxonomic classification
Morphology and physiology
Culture and growth conditions
Isolation, sampling and environmental information
Molecular biology
Strain availability

External links

Information on the name and the taxonomic classification.Name and taxonomic classification

[Ref.: #43860]	Domain	Bacteria
[Ref.: #43860]	Phylum	Firmicutes
[Ref.: #43860]	Class	Bacilli
[Ref.: #43860]	Family	Bacillaceae
[Ref.: #43860]	Genus	Bacillus
[Ref.: #43860]	Species	Bacillus tropicus
[Ref.: #43860]	Strain Designation	N24
[Ref.: #43860]	Type strain

		Prokaryotic Nomenclature Up-to-date (PNU)	Taxonomical status	Literature reference
	Last PNU update	20-11-2019 (DD-MM-YYYY)
[Ref.: #20215]	Domain	Bacteria
[Ref.: #20215]	Phylum	Firmicutes
[Ref.: #20215]	Class	Bacilli		Int. J. Syst. Bacteriol. 38:220
[Ref.: #20215]	Family	Bacillaceae		Int. J. Syst. Bacteriol. 30:225
[Ref.: #20215]	Genus	Bacillus	genus (AL)	Int. J. Syst. Bacteriol. 30:225
[Ref.: #20215]	Species	Bacillus tropicus	sp. nov. (VP)	Int. J. Syst. Evol. Microbiol. 67:2499*
[Ref.: #20215]	Full Scientific Name	Bacillus tropicus Liu et al. 2017

Information on morphological and physiological propertiesMorphology and physiology

[Ref.: #43860]	Gram stain	positive
[Ref.: #43860]	Cell length	2.2-2.6 µm
[Ref.: #43860]	Cell width	1.3-1.6 µm
[Ref.: #43860]	Cell shape	rod-shaped
[Ref.: #43860]	Motility	yes
[Ref.: #43860]	Flagellum arrangement	peritrichous

[Ref.: #43860]	Colony size	2-3 mm
[Ref.: #43860]	Colony color	off-white
[Ref.: #43860]	Colony shape	circular
[Ref.: #43860]	Incubation period	2 days
[Ref.: #43860]	Cultivation medium used	Luria-Bertani agar

	Enzyme	Enzyme activity	EC number
[Ref.: #43860]	arginine dihydrolase	+	3.5.3.6
[Ref.: #43860]	beta-galactosidase	-	3.2.1.23
[Ref.: #43860]	catalase	+	1.11.1.6
[Ref.: #43860]	cytochrome oxidase	+	1.9.3.1
[Ref.: #43860]	gelatinase	+
[Ref.: #43860]	lysine decarboxylase	-	4.1.1.18
[Ref.: #43860]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #43860]	tryptophan deaminase	-	4.1.99.1
[Ref.: #43860]	urease	-	3.5.1.5

Fatty acid profile

Type of FA analysis

whole cell analysis

Incubation medium

Tripticase soy agar

Agar/liquid

agar

Incubation temperature (°C)

Incubation time (d)

Incubation pH

Incubation oxygen condition

Treatment/condition

Software version

Sherlock 6.0B

Library/peak naming table

TSBA 6.0

System

MIS MIDI

Instrument

Agilent 6850 gas chromatograph

Method/protocol

Cutoff value

1.00

Reference

[Ref.: #43860]

Fatty acid	Percentage
anteiso-C13:0	1
anteiso-C15:0	4.4
anteiso-C17:0	1.7
C12:0	1
C14:0	5.5
C14:0 3-OH / iso-C16:1 I	1.9
C16:0	12
C16:1ω11c	1
C16:1ω7c / iso-C15:0 2-OH	9.9
C16:1ω7c alcohol	1.3
iso-C13:0	7.1
iso-C14:0	5.9
iso-C15:0	18.9
iso-C16:0	7.8
iso-C17:0	5.2
iso-C17:1ω10c	3.5
iso-C17:1ω5c	2.6

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43860]		NaCl		growth	0-9	%(w/v)
[Ref.: #43860]		NaCl		optimum	0-0.5	%(w/v)

	Metabolite	Production
[Ref.: #43860]	Acetoin	yes
[Ref.: #43860]	Hydrogen sulfide	no
[Ref.: #43860]	Indole	no

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #43860]		Acetoin	+

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43860]	2-Dehydro-D-gluconate	-	builds acid from
[Ref.: #43860]	5-Dehydro-D-gluconate	-	builds acid from
[Ref.: #43860]	Amygdalin	-	builds acid from
[Ref.: #43860]	Arabinose	-	builds acid from
[Ref.: #43860]	Arbutin	-	builds acid from
[Ref.: #43860]	Carboxymethylcellulose	+	hydrolysis
[Ref.: #43860]	Casein	+	hydrolysis
[Ref.: #43860]	Cellobiose	+	builds acid from
[Ref.: #43860]	Citric acid	+	carbon source
[Ref.: #43860]	D-Adonitol	-	builds acid from
[Ref.: #43860]	D-Arabinose	-	builds acid from
[Ref.: #43860]	D-Arabitol	-	builds acid from
[Ref.: #43860]	D-Fructose	+	builds acid from
[Ref.: #43860]	D-Fucose	-	builds acid from
[Ref.: #43860]	D-Galactose	-	builds acid from
[Ref.: #43860]	D-Glucose	-	builds acid from
[Ref.: #43860]	D-Lyxose	-	builds acid from
[Ref.: #43860]	D-Mannitol	-	builds acid from
[Ref.: #43860]	D-Mannose	-	builds acid from
[Ref.: #43860]	D-Ribose	+	builds acid from
[Ref.: #43860]	D-Sorbitol	-	builds acid from
[Ref.: #43860]	D-Tagatose	-	builds acid from
[Ref.: #43860]	D-Xylose	-	builds acid from
[Ref.: #43860]	Dulcitol	-	builds acid from
[Ref.: #43860]	Erythritol	-	builds acid from
[Ref.: #43860]	Esculin ferric citrate	+	builds acid from
[Ref.: #43860]	Gentiobiose	-	builds acid from
[Ref.: #43860]	Glucose	-	builds acid from
[Ref.: #43860]	Glycerol	+	builds acid from
[Ref.: #43860]	Glycogen	+	builds acid from
[Ref.: #43860]	Inositol	-	builds acid from
[Ref.: #43860]	Inulin	-	builds acid from
[Ref.: #43860]	L-Arabinose	-	builds acid from
[Ref.: #43860]	L-Arabitol	-	builds acid from
[Ref.: #43860]	L-Fucose	-	builds acid from
[Ref.: #43860]	L-Rhamnose	-	builds acid from
[Ref.: #43860]	L-Sorbose	-	builds acid from
[Ref.: #43860]	L-Xylose	-	builds acid from
[Ref.: #43860]	Lactose	-	builds acid from
[Ref.: #43860]	Maltose hydrate	+	builds acid from
[Ref.: #43860]	Mannitol	-	builds acid from
[Ref.: #43860]	Melezitose	-	builds acid from
[Ref.: #43860]	Melibiose	-	builds acid from
[Ref.: #43860]	Methyl alpha-D-glucopyranoside	-	builds acid from
[Ref.: #43860]	Methyl alpha-D-mannoside	-	builds acid from
[Ref.: #43860]	Methyl beta-D-xylopyranoside	-	builds acid from
[Ref.: #43860]	N-Acetylglucosamine	-	builds acid from
[Ref.: #43860]	Potassium gluconate	+	builds acid from
[Ref.: #43860]	Raffinose	-	builds acid from
[Ref.: #43860]	Rhamnose	-	builds acid from
[Ref.: #43860]	Salicin	+	builds acid from
[Ref.: #43860]	Skimmed milk	+	hydrolysis
[Ref.: #43860]	Sorbitol	-	builds acid from
[Ref.: #43860]	Starch	+	builds acid from
[Ref.: #43860]	Starch	+	hydrolysis
[Ref.: #43860]	Sucrose	-	builds acid from
[Ref.: #43860]	Trehalose	+	builds acid from
[Ref.: #43860]	Turanose	-	builds acid from
[Ref.: #43860]	Xylitol	-	builds acid from
	Only first 10 entries are displayed. Click here to see all.

[Ref.: #43860]

Oxygen tolerance

facultative anaerobe

[Ref.: #43860]	Spore description	central elliptical endospore
[Ref.: #43860]	Type of spore	endospore
[Ref.: #43860]	Ability of spore formation	yes

Information on culture and growth conditionsCulture and growth conditions

[Ref.: #43860]

Culture medium

Luria-Bertani agar

[Ref.: #43860]

Culture medium growth

[Ref.: #43860]

Culture medium

Trypticase soy agar

[Ref.: #43860]

Culture medium growth

	Temperatures	Kind of temperature		Temperature
[Ref.: #43860]			growth	15-45 ̊C
[Ref.: #43860]			optimum	30 ̊C

[Ref.: #43860]

Temperature range

mesophilic

	pH	Kind of pH		pH
[Ref.: #43860]			optimum	6
[Ref.: #43860]			growth	5.0-9.0

Information on isolation source, the sampling and environmental conditionsIsolation, sampling and environmental information

[Ref.: #43860]

Sample type/isolated from

sediment of the South China Sea

Isolation sources categories

#Environmental	#Aquatic	#Marine
#Environmental	#Aquatic	#Sediment

What are isolation sources categories?

* marker position based on {}

Information on genomic background e.g. entries in nucleic sequence databassMolecular biology

[Ref.: #43860]

GC-content

35.2 mol%

genome sequence analysis

Sequence information:

	Sequence database	Sequence accession description	Sequence accession number	Sequence type	Sequence length (bp)	Associated NCBI tax ID
[Ref.: #43860]	GenBank, EMBL, DDBJ	16S rRNA gene sequence	KJ812435	16S
[Ref.: #43860]	GenBank, EMBL, DDBJ	whole genome sequence	MACG00000000	WGS

Availability in culture collectionsStrain availability

[Ref.: #43860]

Culture collection no.

MCCC 1A01406, KCTC 33711, LMG 28874

References

#20215

Leibniz Institute DSMZ D.Gleim, M.Kracht, N.Weiss et. al.: Prokaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes since .

#43860

Yang Liu, Juan Du, Qiliang Lai, Runying Zeng, Dezan Ye, Jun Xu, Zongze Shao: Proposal of nine novel species of the Bacillus cereus group. IJSEM 67: 2499 - 2508 2017 ( DOI 10.1099/ijsem.0.001821 )

* These References are textmined

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