Name: Gracilimonas halophila WDS2C40
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 140707

Type strain:

Species: Gracilimonas halophila

Strain Designation: WDS2C40

Culture col. no.: KCTC 52042, MCCC 1H00135

Strain history: <- Zongjun Du, Shandong Univ., China

NCBI tax ID(s): 1834464 (species)

Section

Gracilimonas halophila WDS2C40 is a facultative anaerobe, Gram-negative, rod-shaped bacterium that forms circular colonies and was isolated from marine solar saltern.

colony-forming
Gram-negative
rod-shaped
facultative anaerobe
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Balneolota*
	Class	*Balneolia*
	Order	*Balneolales*
	Family	*Balneolaceae*
	Genus	*Gracilimonas*
	Species	**Gracilimonas halophila**
	Full Scientific Name (LPSN)	Gracilimonas halophila Lu et al. 2017

Information on morphological and physiological properties Morphology

[Ref.: #43480]	Gram stain	negative
[Ref.: #67771]	Gram stain	negative

[Ref.: #43480]	Cell length	4-9 µm

[Ref.: #43480]	Cell width	0.4-0.5 µm

[Ref.: #43480]	Cell shape	rod-shaped
[Ref.: #67771]	Cell shape	rod-shaped

[Ref.: #43480]	Motility	no
[Ref.: #67771]	Motility	no

[Ref.: #43480]	Colony size	1 mm
[Ref.: #43480]	Colony color	opaque, pink
[Ref.: #43480]	Colony shape	circular
[Ref.: #43480]	Incubation period	4 days
[Ref.: #43480]	Cultivation medium used	Modified MA

Information on culture and growth conditions Culture and growth conditions

[Ref.: #43480]

Culture medium

Modified MA

[Ref.: #43480]

Culture medium growth

		Temperature
[Ref.: #43480]	growth	20-42 °C
[Ref.: #43480]	optimum	37-40 °C
[Ref.: #67771]	growth	33-37 °C

[Ref.: #67771]

Temperature range

mesophilic

	pH	Kind of pH		pH
[Ref.: #43480]			optimum	7.5-8
[Ref.: #43480]			growth	7-8.5

Information on physiology and metabolism Physiology and metabolism

[Ref.: #43480]	Oxygen tolerance	facultative anaerobe
[Ref.: #67771]	Oxygen tolerance	aerobe

[Ref.: #43480]	Ability of spore formation	no
[Ref.: #67771]	Ability of spore formation	no

[Ref.: #43480]

Halophily / tolerance level

moderately halophilic

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43480]		NaCl		growth	2-16	%(w/v)
[Ref.: #43480]		NaCl		optimum	6-8	%(w/v)

[Ref.: #67771]

Observation

quinones: MK-7

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43480]	2-oxoglutarate ChEBI	+	carbon source
[Ref.: #43480]	2-oxoglutarate ChEBI	+	energy source
[Ref.: #43480]	2-oxoglutarate ChEBI	+	oxidation
[Ref.: #43480]	acetoacetate ChEBI	+	carbon source
[Ref.: #43480]	acetoacetate ChEBI	+	energy source
[Ref.: #43480]	acetoacetate ChEBI	+	oxidation
[Ref.: #43480]	D-arabitol ChEBI	+	builds acid from
[Ref.: #43480]	D-fructose ChEBI	+	builds acid from
[Ref.: #43480]	D-fructose 6-phosphate ChEBI	+	carbon source
[Ref.: #43480]	D-fructose 6-phosphate ChEBI	+	energy source
[Ref.: #43480]	D-fructose 6-phosphate ChEBI	+	oxidation
[Ref.: #43480]	D-glucose ChEBI	+	builds acid from
[Ref.: #43480]	D-glucuronate ChEBI	+	carbon source
[Ref.: #43480]	D-glucuronate ChEBI	+	energy source
[Ref.: #43480]	D-glucuronate ChEBI	+	oxidation
[Ref.: #43480]	D-tagatose ChEBI	+	builds acid from
[Ref.: #43480]	gelatin ChEBI	+	hydrolysis
[Ref.: #43480]	glucuronamide ChEBI	+	carbon source
[Ref.: #43480]	glucuronamide ChEBI	+	energy source
[Ref.: #43480]	glucuronamide ChEBI	+	oxidation
[Ref.: #43480]	glycogen ChEBI	+	builds acid from
[Ref.: #43480]	hypoxanthine ChEBI	-	hydrolysis
[Ref.: #43480]	maltose ChEBI	+	builds acid from
[Ref.: #43480]	melibiose ChEBI	+	builds acid from
[Ref.: #43480]	nitrate ChEBI	-	reduction
[Ref.: #43480]	potassium 5-dehydro-D-gluconate	+	builds acid from
[Ref.: #43480]	raffinose ChEBI	+	builds acid from
[Ref.: #43480]	starch ChEBI	+	builds acid from
[Ref.: #43480]	starch ChEBI	+	hydrolysis
[Ref.: #43480]	sucrose ChEBI	+	builds acid from
[Ref.: #43480]	trehalose ChEBI	+	builds acid from
[Ref.: #43480]	turanose ChEBI	+	carbon source
[Ref.: #43480]	turanose ChEBI	+	energy source
[Ref.: #43480]	turanose ChEBI	+	oxidation
[Ref.: #43480]	tween 20 ChEBI	-	hydrolysis
[Ref.: #43480]	tween 40 ChEBI	-	hydrolysis
[Ref.: #43480]	tween 60 ChEBI	+	hydrolysis
[Ref.: #43480]	tween 80 ChEBI	-	hydrolysis
[Ref.: #43480]	xanthine ChEBI	-	hydrolysis
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #43480]	acid phosphatase	+	3.1.3.2
[Ref.: #43480]	alkaline phosphatase	+	3.1.3.1
[Ref.: #43480]	alpha-chymotrypsin	+	3.4.21.1
[Ref.: #43480]	alpha-galactosidase	-	3.2.1.22
[Ref.: #43480]	alpha-glucosidase	-	3.2.1.20
[Ref.: #43480]	alpha-mannosidase	-	3.2.1.24
[Ref.: #43480]	beta-D-fucosidase	-	3.2.1.38
[Ref.: #43480]	beta-galactosidase	-	3.2.1.23
[Ref.: #43480]	beta-glucosidase	-	3.2.1.21
[Ref.: #43480]	beta-glucuronidase	-	3.2.1.31
[Ref.: #43480]	catalase	+	1.11.1.6
[Ref.: #43480]	cystine arylamidase	+	3.4.11.3
[Ref.: #43480]	cytochrome oxidase	-	1.9.3.1
[Ref.: #43480]	esterase Lipase (C 8)	-
[Ref.: #43480]	leucine arylamidase	+	3.4.11.1
[Ref.: #43480]	lipase (C 14)	-
[Ref.: #43480]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #43480]	naphthol-AS-BI-phosphohydrolase	-
[Ref.: #43480]	trypsin	+	3.4.21.4
[Ref.: #43480]	valine arylamidase	+
	Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #43480]	Sample type/isolated from	marine solar saltern
[Ref.: #43480]	Geographic location (country and/or sea, region)	Weihai coast, Shandong Province
[Ref.: #43480]	Country	China
[Ref.: #43480]	Country ISO 3 Code	CHN
[Ref.: #43480]	Continent	Asia

[Ref.: #67771]	Sample type/isolated from	From marine solar saltern
[Ref.: #67771]	Geographic location (country and/or sea, region)	the coastal area of Weihai
[Ref.: #67771]	Country	China
[Ref.: #67771]	Country ISO 3 Code	CHN
[Ref.: #67771]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Marine
#Condition	#Saline	-
#Engineered	#Industrial	#Industrial production
#Environmental	#Terrestrial	#Coast

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence KU987442 (>99% sequence identity) for Gracilimonas halophila subclade from Microbeatlas

Aquatic Samples	40
Soil Samples
Animal Samples
Plant Samples
Total Samples	40

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #43480]	16S rRNA gene sequence	KU987442		GenBank

[Ref.: #43480]	GC-content	41.7 mol%	high performance liquid chromatography (HPLC)
[Ref.: #67771]	GC-content	41.7 mol%

Availability in culture collections External links

[Ref.: #43480]

Culture collection no.

KCTC 52042, MCCC 1H00135

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Gracilimonas amylolytica sp. nov., isolated from deep-sea sediment.	Wu YH, Yan J, Fang C, Huo YY, Ma WL, Zhang DM, Wang CS, Xu XW	Int J Syst Evol Microbiol	10.1099/ijsem.0.002734	2018	*
Phylogeny	Gracilimonas halophila sp. nov., isolated from a marine solar saltern.	Lu DC, Xia J, Dunlap CA, Rooney AP, Du ZJ	Int J Syst Evol Microbiol	10.1099/ijsem.0.002093	2017	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#43480

De-Chen Lu, Jun Xia, Christopher A. Dunlap, Alejandro P. Rooney, Zong-Jun Du: Gracilimonas halophila sp. nov., isolated from a marine solar saltern. IJSEM 67: 3251 - 3255 2017 ( DOI 10.1099/ijsem.0.002093 , PubMed 28829029 )

#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

* These data were automatically processed and therefore are not curated

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