Name: Metabacillus iocasae S36
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 140408

Type strain:

Species: Metabacillus iocasae

Strain Designation: S36

Culture col. no.: DSM 104297, CGMCC 1.16030, KCTC 33864

Strain history: <- H. Wang; Chinese Academy of Sciences, Qingdao, P.R. CHINA; S36

NCBI tax ID(s): 2291674 (species)

SI-ID 405018

DSM 104297

Special Collections

Section

Metabacillus iocasae S36 is an aerobe, spore-forming, Gram-positive bacterium that forms circular colonies and was isolated from deep-sea sediment from a hydrothermal field.

colony-forming
Gram-positive
motile
rod-shaped
spore-forming
aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Metabacillus*
	Species	**Metabacillus iocasae**
	Full Scientific Name (LPSN)	Metabacillus iocasae (Wang et al. 2017) Gupta et al. 2020

Synonym

Bacillus iocasae

Information on morphological and physiological properties Morphology

[Ref.: #43901]	Gram stain	positive

[Ref.: #43901]	Cell length	2.9-4.5 µm

[Ref.: #43901]	Cell width	0.7-1.0 µm

[Ref.: #43901]	Cell shape	rod-shaped

[Ref.: #43901]	Motility	yes

[Ref.: #43901]	Flagellum arrangement	peritrichous

[Ref.: #43901]	Colony size	1-3 mm
[Ref.: #43901]	Colony color	beige
[Ref.: #43901]	Colony shape	circular
[Ref.: #43901]	Incubation period	3 days
[Ref.: #43901]	Cultivation medium used	Marine agar 2216E

Information on culture and growth conditions Culture and growth conditions

[Ref.: #43901]

Culture medium

Marine agar 2216E

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Culture medium growth

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Culture medium

BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

expand / minimize

		Temperature
[Ref.: #43015]	growth	30 °C
[Ref.: #43901]	growth	16-50 °C
[Ref.: #43901]	optimum	28 °C

	pH	Kind of pH		pH
[Ref.: #43901]			optimum	8
[Ref.: #43901]			growth	6.0-10.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #43901]

Oxygen tolerance

aerobe

[Ref.: #43901]	Spore description	oval to round, centrally located in unswollen sporangia
[Ref.: #43901]	Type of spore	endospore
[Ref.: #43901]	Ability of spore formation	yes
[Ref.: #69480]	Ability of spore formation	yes Confidence in %94.621
[Ref.: #69481]	Ability of spore formation	yes Confidence in %100

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43901]		NaCl		growth	0-11	%(w/v)
[Ref.: #43901]		NaCl		optimum	7	%(w/v)

[Ref.: #43901]

Observation

quinone MK-7

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43901]	2-oxoglutarate ChEBI	+	carbon source
[Ref.: #43901]	2-oxopentanoate ChEBI	+	carbon source
[Ref.: #43901]	3-O-methyl-D-glucose ChEBI	+	carbon source
[Ref.: #43901]	acetate ChEBI	+	carbon source
[Ref.: #43901]	alpha-D-glucose ChEBI	+	carbon source
[Ref.: #43901]	arabinose ChEBI	-	assimilation
[Ref.: #43901]	arbutin ChEBI	-	builds acid from
[Ref.: #43901]	beta-hydroxybutyrate ChEBI	+	carbon source
[Ref.: #43901]	cellobiose ChEBI	+	builds acid from
[Ref.: #43901]	cyclodextrin ChEBI	+	carbon source
[Ref.: #43901]	D-alanine ChEBI	+	carbon source
[Ref.: #43901]	D-arabinose ChEBI	-	builds acid from
[Ref.: #43901]	D-arabitol ChEBI	-	builds acid from
[Ref.: #43901]	D-fructose ChEBI	+	builds acid from
[Ref.: #43901]	D-fructose ChEBI	+	carbon source
[Ref.: #43901]	D-fucose ChEBI	-	builds acid from
[Ref.: #43901]	D-galactose ChEBI	-	builds acid from
[Ref.: #43901]	D-glucose ChEBI	+	builds acid from
[Ref.: #43901]	D-lyxose ChEBI	-	builds acid from
[Ref.: #43901]	D-mannitol ChEBI	-	builds acid from
[Ref.: #43901]	D-mannose ChEBI	-	builds acid from
[Ref.: #43901]	D-mannose ChEBI	+/-	carbon source
[Ref.: #43901]	D-ribose ChEBI	+/-	builds acid from
[Ref.: #43901]	D-sorbitol ChEBI	-	builds acid from
[Ref.: #43901]	D-tagatose ChEBI	-	builds acid from
[Ref.: #43901]	D-xylose ChEBI	-	builds acid from
[Ref.: #43901]	erythritol ChEBI	-	builds acid from
[Ref.: #43901]	esculin ferric citrate	+	builds acid from
[Ref.: #43901]	galactitol ChEBI	-	builds acid from
[Ref.: #43901]	gelatin ChEBI	+	hydrolysis
[Ref.: #43901]	glucose ChEBI	-	fermentation
[Ref.: #43901]	glucose ChEBI	+	assimilation
[Ref.: #43901]	glycogen ChEBI	+	builds acid from
[Ref.: #43901]	glycyl-l-glutamate ChEBI	+/-	carbon source
[Ref.: #43901]	inulin ChEBI	-	builds acid from
[Ref.: #43901]	L-arabinose ChEBI	-	builds acid from
[Ref.: #43901]	L-arabitol ChEBI	-	builds acid from
[Ref.: #43901]	L-fucose ChEBI	-	builds acid from
[Ref.: #43901]	L-pyroglutamic acid ChEBI	+/-	carbon source
[Ref.: #43901]	L-rhamnose ChEBI	-	builds acid from
[Ref.: #43901]	L-serine ChEBI	+/-	carbon source
[Ref.: #43901]	L-sorbose ChEBI	-	builds acid from
[Ref.: #43901]	L-xylose ChEBI	-	builds acid from
[Ref.: #43901]	lactamide ChEBI	+/-	carbon source
[Ref.: #43901]	lactose ChEBI	-	builds acid from
[Ref.: #43901]	maltose ChEBI	+	assimilation
[Ref.: #43901]	maltose ChEBI	+	carbon source
[Ref.: #43901]	maltose hydrate	+	builds acid from
[Ref.: #43901]	mannitol ChEBI	-	assimilation
[Ref.: #43901]	mannose ChEBI	-	assimilation
[Ref.: #43901]	mannose ChEBI	-	builds acid from
[Ref.: #43901]	melezitose ChEBI	+/-	builds acid from
[Ref.: #43901]	melibiose ChEBI	-	builds acid from
[Ref.: #43901]	methyl alpha-D-glucopyranoside ChEBI	+	carbon source
[Ref.: #43901]	myo-inositol ChEBI	-	builds acid from
[Ref.: #43901]	N-acetylglucosamine ChEBI	+	assimilation
[Ref.: #43901]	N-acetylglucosamine ChEBI	+	builds acid from
[Ref.: #43901]	nitrate ChEBI	-	reduction
[Ref.: #43901]	pyruvate ChEBI	+	carbon source
[Ref.: #43901]	raffinose ChEBI	+/-	builds acid from
[Ref.: #43901]	ribitol ChEBI	-	builds acid from
[Ref.: #43901]	salicin ChEBI	-	builds acid from
[Ref.: #43901]	sodium citrate ChEBI	-	assimilation
[Ref.: #43901]	starch ChEBI	+	builds acid from
[Ref.: #43901]	starch ChEBI	+	hydrolysis
[Ref.: #43901]	sucrose ChEBI	+	builds acid from
[Ref.: #43901]	sucrose ChEBI	+	carbon source
[Ref.: #43901]	thymidine ChEBI	+	carbon source
[Ref.: #43901]	trehalose ChEBI	+	builds acid from
[Ref.: #43901]	trehalose ChEBI	+	carbon source
[Ref.: #43901]	tween 80 ChEBI	+	hydrolysis
[Ref.: #43901]	uridine ChEBI	+/-	carbon source
[Ref.: #43901]	uridine 5'-monophosphate ChEBI	+	carbon source
[Ref.: #43901]	xylitol ChEBI	-	builds acid from
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #43901]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #43901]	acid phosphatase	-	3.1.3.2
[Ref.: #43901]	alkaline phosphatase	-	3.1.3.1
[Ref.: #43901]	alpha-fucosidase	-	3.2.1.51
[Ref.: #43901]	alpha-galactosidase	-	3.2.1.22
[Ref.: #43901]	alpha-glucosidase	+	3.2.1.20
[Ref.: #43901]	alpha-mannosidase	-	3.2.1.24
[Ref.: #43901]	beta-galactosidase	+	3.2.1.23
[Ref.: #43901]	beta-glucosidase	-	3.2.1.21
[Ref.: #43901]	beta-glucuronidase	-	3.2.1.31
[Ref.: #43901]	chymotrypsin	+	3.4.4.5
[Ref.: #43901]	cystine arylamidase	+	3.4.11.3
[Ref.: #43901]	esterase (C 4)	+
[Ref.: #43901]	esterase Lipase (C 8)	+
[Ref.: #43901]	leucine arylamidase	+	3.4.11.1
[Ref.: #43901]	lipase (C 14)	-
[Ref.: #43901]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #43901]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #43901]	trypsin	-	3.4.21.4
[Ref.: #43901]	urease	-	3.5.1.5
[Ref.: #43901]	valine arylamidase	-
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #43901]

Fatty acid	Percentage
C15:0 iso	31.9
C15:0 anteiso	17
C16:0 iso	6
C14:0 iso	5.7
C16:1ω7c alcohol	5.5
C16:1ω11c	5.1
C17:1 I / anteiso-C17:1 B	4.7
C17:0 anteiso	4.6
C16:0	3.7
C17:0 iso	3.2
C17:1 iso ω10c	2.5
C14:0	2.4
C16:1ω7c / C16:1ω6c	2
C16:0 N-alcohol	1.8
C18:1ω9c	0.8
C17:1 anteiso A	0.4
C17:0	0.4

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #43015]	Sample type/isolated from	deep-sea sediment from a hydrothermal field
[Ref.: #43015]	Geographic location (country and/or sea, region)	Manus Basin, Pacmanus hydrothermal field (151° 40' 37.970'' E 3° 44' 03.222'' S)
[Ref.: #43015]	Country	Papua New Guinea
[Ref.: #43015]	Country ISO 3 Code	PNG
[Ref.: #43015]	Continent	Australia and Oceania
[Ref.: #43015]	Geographic location	-4.6283°/151.6770°

[Ref.: #43901]	Sample type/isolated from	marine sediment sample collected in Pacmanus hydrothermal field, Manus Basin, Papua New Guinea, at a depth of 1851 m
[Ref.: #43901]	Geographic location (country and/or sea, region)	Pacmanus hydrothermal field, Manus Basin
[Ref.: #43901]	Country	Papua New Guinea
[Ref.: #43901]	Country ISO 3 Code	PNG
[Ref.: #43901]	Continent	Australia and Oceania
[Ref.: #43901]	Geographic location	-3.7342°/151.6770°
[Ref.: #43901]	Enrichment culture	Marine agar 2216E
* marker position based on {}

Isolation sources categories

#Condition	#Thermophilic (>45°C)	-
#Environmental	#Aquatic	#Hydrothermal vent
#Environmental	#Aquatic	#Marine
#Environmental	#Aquatic	#Sediment

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #43015]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #43015]	Metabacillus iocasae 16S ribosomal RNA gene, partial sequence	KY462210	1510	GenBank ENA	2291674 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Metabacillus iocasae DSM 104297	GCA_016909075	contig	GenBank ENA	2291674 tax ID	*
[Ref.: #66792]	Bacillus iocasae strain DSM 104297	2291674.3	wgs	PATRIC	2291674 tax ID	*
[Ref.: #66792]	Bacillus iocasae DSM 104297	2901267961	draft	JGI IMG/M	2291674 tax ID	*

[Ref.: #43901]	GC-content	43 mol%	high performance liquid chromatography (HPLC)
[Ref.: #43015]	GC-content	43.0 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Bacillus iocasae strain DSM 104297 PATRIC: 2291674.3
[Ref.: #69481]	spore-forming	spore-formingⓘ	yes	100	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Bacillus iocasae strain DSM 104297 PATRIC: 2291674.3
[Ref.: #69480]	gram-positive	gram-positiveⓘ	yes	80.121	yes
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	93.066	yes
[Ref.: #69480]	spore-forming	spore-formingⓘ	yes	94.621	yes
[Ref.: #69480]	aerobic	aerobicⓘ	yes	79.93	yes
[Ref.: #69480]	thermophilic	thermophileⓘ	no	85.179	no
[Ref.: #69480]	flagellated	motile2+ⓘ	yes	83.965	no

Availability in culture collections External links

[Ref.: #43015]

Culture collection no.

DSM 104297, CGMCC 1.16030, KCTC 33864

[Ref.: #96684]

SI-ID 405018

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Bacillus iocasae sp. nov., isolated from Pacmanus hydrothermal field, Manus Basin.	Wang HL, Zhang J, Sun L	Int J Syst Evol Microbiol	10.1099/ijsem.0.002164	2017	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#43015

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 104297

#43901

Hai-liang Wang, Jian Zhang, Li Sun: Bacillus iocasae sp. nov., isolated from Pacmanus hydrothermal field, Manus Basin. IJSEM 67: 3547 - 3552 2017 ( DOI 10.1099/ijsem.0.002164 , PubMed 28866991 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#96684

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID405018.1 )

* These data were automatically processed and therefore are not curated

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Bacillus iocasae strain DSM 104297

Bacillus iocasae strain DSM 104297

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