Metabacillus iocasae (DSM 104297, CGMCC 1.16030, KCTC 33864)

Name: Metabacillus iocasae (DSM 104297, CGMCC 1.16030, KCTC 33864)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 140408

Type strain

Strain Designation S36

Culture col. no. DSM 104297 CGMCC 1.16030 KCTC 33864 S36

NCBI tax ID(s) 2291674

Special Collections DSMZ KCTC Literature strains

History DSMZ <- H. Wang; Chinese Academy of Sciences, Qingdao, P.R. CHINA; S36

Links

Metabacillus iocasae S36 is an aerobe, spore-forming, Gram-positive bacterium that forms circular colonies and was isolated from deep-sea sediment from a hydrothermal field.

spore-forming Gram-positive motile rod-shaped colony-forming aerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 405018

KCTC 33864,DSM 104297

@ref 20215

Last LPSN update 2026-05-29 08:39:42

Domain Bacteria

Phylum Bacillota

Class Bacilli

Order Caryophanales

Family Bacillaceae

Genus Metabacillus

Species Metabacillus iocasae

Full scientific name Metabacillus iocasae (Wang et al. 2017) Gupta et al. 2020

Synonyms (2)

Priestia iocasae
Bacillus iocasae

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Motility	Flagellum arrangement
43901	positive	2.9-4.5 µm	0.7-1.0 µm	rod-shaped		peritrichous

Colony morphology

@ref	Colony size	Colony color	Colony shape	Incubation period	Medium used
43901	1-3 mm	beige	circular	3 days	Marine agar 2216E

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
43901	Marine agar 2216E
43015	BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)		Medium recipe at MediaDive	Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
43015	positive	growth	30
43901	positive	growth	16-50
43901	positive	optimum	28

Culture pH

@ref	Ability	Type	PH	PH range
43901	positive	optimum	8
43901	positive	growth	6.0-10.0	alkaliphile

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
43901	aerobe
125439	obligate aerobe	97.804

Spore formation

@ref	Spore description	Type of spore	Confidence
43901	oval to round, centrally located in unswollen sporangia	endospore
125438			94.621
125439			90.654

Halophily

@ref	Salt	Growth	Tested relation	Concentration
43901	NaCl	positive	growth	0-11 %(w/v)
43901	NaCl	positive	optimum	7 %(w/v)

Observation

43901

Observationquinone MK-7

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
43901	30916 ChEBI	2-oxoglutarate	+	carbon source
43901	28644 ChEBI	2-oxopentanoate	+	carbon source
43901	73918 ChEBI	3-O-methyl-D-glucose	+	carbon source
43901	30089 ChEBI	acetate	+	carbon source
43901	17925 ChEBI	alpha-D-glucose	+	carbon source
43901	22599 ChEBI	arabinose	-	assimilation
43901	18305 ChEBI	arbutin	-	builds acid from
43901	8295 ChEBI	beta-hydroxybutyrate	+	carbon source
43901	17057 ChEBI	cellobiose	+	builds acid from
43901	23456 ChEBI	cyclodextrin	+	carbon source
43901	15570 ChEBI	D-alanine	+	carbon source
43901	17108 ChEBI	D-arabinose	-	builds acid from
43901	18333 ChEBI	D-arabitol	-	builds acid from
43901	15824 ChEBI	D-fructose	+	builds acid from
43901	15824 ChEBI	D-fructose	+	carbon source
43901	28847 ChEBI	D-fucose	-	builds acid from
43901	12936 ChEBI	D-galactose	-	builds acid from
43901	17634 ChEBI	D-glucose	+	builds acid from
43901	62318 ChEBI	D-lyxose	-	builds acid from
43901	16899 ChEBI	D-mannitol	-	builds acid from
43901	16024 ChEBI	D-mannose	-	builds acid from
43901	16024 ChEBI	D-mannose	+/-	carbon source
43901	16988 ChEBI	D-ribose	+/-	builds acid from
43901	17924 ChEBI	D-sorbitol	-	builds acid from
43901	16443 ChEBI	D-tagatose	-	builds acid from
43901	65327 ChEBI	D-xylose	-	builds acid from
43901	17113 ChEBI	erythritol	-	builds acid from
43901		esculin ferric citrate	+	builds acid from
43901	16813 ChEBI	galactitol	-	builds acid from
43901	5291 ChEBI	gelatin	+	hydrolysis
43901	17234 ChEBI	glucose	-	fermentation
43901	17234 ChEBI	glucose	+	assimilation
43901	28087 ChEBI	glycogen	+	builds acid from
43901	73784 ChEBI	glycyl-l-glutamate	+/-	carbon source
43901	15443 ChEBI	inulin	-	builds acid from
43901	30849 ChEBI	L-arabinose	-	builds acid from
43901	18403 ChEBI	L-arabitol	-	builds acid from
43901	18287 ChEBI	L-fucose	-	builds acid from
43901	18183 ChEBI	L-pyroglutamic acid	+/-	carbon source
43901	62345 ChEBI	L-rhamnose	-	builds acid from
43901	17115 ChEBI	L-serine	+/-	carbon source
43901	17266 ChEBI	L-sorbose	-	builds acid from
43901	65328 ChEBI	L-xylose	-	builds acid from
43901	75144 ChEBI	lactamide	+/-	carbon source
43901	17716 ChEBI	lactose	-	builds acid from
43901	17306 ChEBI	maltose	+	assimilation
43901	17306 ChEBI	maltose	+	carbon source
43901		maltose hydrate	+	builds acid from
43901	29864 ChEBI	mannitol	-	assimilation
43901	37684 ChEBI	mannose	-	assimilation
43901	37684 ChEBI	mannose	-	builds acid from
43901	6731 ChEBI	melezitose	+/-	builds acid from
43901	28053 ChEBI	melibiose	-	builds acid from
43901	320061 ChEBI	methyl alpha-D-glucopyranoside	+	carbon source
43901	17268 ChEBI	myo-inositol	-	builds acid from
43901	506227 ChEBI	N-acetylglucosamine	+	assimilation
43901	506227 ChEBI	N-acetylglucosamine	+	builds acid from
43901	17632 ChEBI	nitrate	-	reduction
43901	15361 ChEBI	pyruvate	+	carbon source
43901	16634 ChEBI	raffinose	+/-	builds acid from
43901	15963 ChEBI	ribitol	-	builds acid from
43901	17814 ChEBI	salicin	-	builds acid from
43901	53258 ChEBI	sodium citrate	-	assimilation
43901	28017 ChEBI	starch	+	builds acid from
43901	28017 ChEBI	starch	+	hydrolysis
43901	17992 ChEBI	sucrose	+	builds acid from
43901	17992 ChEBI	sucrose	+	carbon source
43901	17748 ChEBI	thymidine	+	carbon source
43901	27082 ChEBI	trehalose	+	builds acid from
43901	27082 ChEBI	trehalose	+	carbon source
43901	53426 ChEBI	tween 80	+	hydrolysis
43901	16704 ChEBI	uridine	+/-	carbon source
43901	16695 ChEBI	uridine 5'-monophosphate	+	carbon source
43901	17151 ChEBI	xylitol	-	builds acid from

Metabolite production

@ref	Chebi-ID	Metabolite	Production
43901	35581 ChEBI	indole

Enzymes

@ref	Value	Activity	Ec
43901	acid phosphatase	-	3.1.3.2
43901	alkaline phosphatase	-	3.1.3.1
43901	alpha-fucosidase	-	3.2.1.51
43901	alpha-galactosidase	-	3.2.1.22
43901	alpha-glucosidase	+	3.2.1.20
43901	alpha-mannosidase	-	3.2.1.24
43901	beta-galactosidase	+	3.2.1.23
43901	beta-glucosidase	-	3.2.1.21
43901	beta-glucuronidase	-	3.2.1.31
43901	chymotrypsin	+	3.4.4.5
43901	cystine arylamidase	+	3.4.11.3
43901	esterase (C 4)	+
43901	esterase Lipase (C 8)	+
43901	leucine arylamidase	+	3.4.11.1
43901	lipase (C 14)	-
43901	N-acetyl-beta-glucosaminidase	-	3.2.1.52
43901	naphthol-AS-BI-phosphohydrolase	+
43901	trypsin	-	3.4.21.4
43901	urease	-	3.5.1.5
43901	valine arylamidase	-

Fatty acid profile

Metadata FA analysis

@ref

43901

Fatty acid	Percentage
C15:0 iso	31.9
C15:0 anteiso	17
C16:0 iso	6
C14:0 iso	5.7
C16:1ω7c alcohol	5.5
C16:1ω11c	5.1
C17:1 I / anteiso-C17:1 B	4.7
C17:0 anteiso	4.6
C16:0	3.7
C17:0 iso	3.2
C17:1 iso ω10c	2.5
C14:0	2.4
C16:1ω7c / C16:1ω6c	2
C16:0 N-alcohol	1.8
C18:1ω9c	0.8
C17:1 anteiso A	0.4
C17:0	0.4

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Condition	#Thermophilic (>45°C)	-
#Environmental	#Aquatic	#Hydrothermal vent
#Environmental	#Aquatic	#Marine
#Environmental	#Aquatic	#Sediment

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent	Latitude	Longitude	Enrichment culture
43015	deep-sea sediment from a hydrothermal field	Manus Basin, Pacmanus hydrothermal field (151° 40' 37.970'' E 3° 44' 03.222'' S)	Papua New Guinea	PNG	Australia and Oceania	-4.6283	151.677 -4.6283/151.677
43901	marine sediment sample collected in Pacmanus hydrothermal field, Manus Basin, Papua New Guinea, at a depth of 1851 m	Pacmanus hydrothermal field, Manus Basin	Papua New Guinea	PNG	Australia and Oceania	-3.73423	151.677 -3.73423/151.677	Marine agar 2216E

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
43015	1	Risk group (German classification)

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM1690907v1 assembly for Priestia iocasae DSM 104297	contig	GCA_016909075	2291674.3	2901267961	2291674	74.54

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
43015	Metabacillus iocasae 16S ribosomal RNA gene, partial sequence	KY462210	1510		2291674
124043	Bacillus iocasae strain CGMCC 1.16030 16S ribosomal RNA gene, partial sequence.	MT759865	1347		2291674

GC content

@ref	GC-content (mol%)	Method
43901	43	high performance liquid chromatography (HPLC)
43015	43.0

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM1690907v1 assembly for Priestia iocasae DSM 104297
NCBI: GCA_016909075

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	97.80	no
125439	gram_stain	BacteriaNetⓘ	variable	58.24	no
125439	motility	BacteriaNetⓘ	yes	85.56	no
125439	spore_formation	BacteriaNetⓘ	yes	90.65	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Bacillus iocasae strain DSM 104297
PATRIC: 2291674.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	80.12	yes
125438	anaerobic	anaerobicⓘ	no	93.07	yes
125438	spore-forming	spore-formingⓘ	yes	94.62	yes
125438	aerobic	aerobicⓘ	yes	79.93	yes
125438	thermophilic	thermophileⓘ	no	85.18	no
125438	flagellated	motile2+ⓘ	yes	83.97	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Bacillus iocasae sp. nov., isolated from Pacmanus hydrothermal field, Manus Basin.	Wang HL, Zhang J, Sun L	Int J Syst Evol Microbiol	10.1099/ijsem.0.002164	2017

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#43015	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 104297
#43901	Hai-liang Wang, Jian Zhang, Li Sun: Bacillus iocasae sp. nov., isolated from Pacmanus hydrothermal field, Manus Basin. IJSEM 67: 3547 - 3552 2017 ( DOI 10.1099/ijsem.0.002164 , PubMed 28866991 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Metabacillus iocasae (DSM 104297, CGMCC 1.16030, KCTC 33864)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Observation

Metabolite utilization

Metabolite production

Enzymes

Fatty acid profile

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_016909075

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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