Name: Sporosalibacterium tautonense MRo-4
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 140261

Type strain:

Species: Sporosalibacterium tautonense

Strain Designation: MRo-4

Culture col. no.: DSM 28179, VKM B-2948

Strain history: <- O. A. Podosokorskaya, Winogradsky Inst. Microbiol., RAS, Moscow, Russia; MRo-4 <- O. A. Podosokorskaya {2013}

NCBI tax ID(s): 1859103 (species)

SI-ID 405281

DSM 28179

Section

Sporosalibacterium tautonense MRo-4 is an obligate anaerobe, moderately halophilic, organotroph bacterium that was isolated from microbial mat developed under the flow of subsurface water in a gold mine at a depth of 3.54 km below the surface.

Gram-positive
rod-shaped
spore-forming
moderately halophilic
obligate anaerobe
organotroph
thermophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Clostridia*
	Order	*Eubacteriales*
	Family	*Clostridiaceae*
	Genus	*Sporosalibacterium*
	Species	**Sporosalibacterium tautonense**
	Full Scientific Name (LPSN)	Sporosalibacterium tautonense Podosokorskaya et al. 2017

Information on morphological and physiological properties Morphology

[Ref.: #43213]	Gram stain	positive

[Ref.: #43213]	Cell length	5-20 µm

[Ref.: #43213]	Cell width	0.2-0.3 µm

[Ref.: #43213]	Cell shape	rod-shaped

Information on culture and growth conditions Culture and growth conditions

[Ref.: #42868]

Culture medium

MARINITOGA MEDIUM (DSMZ Medium 904)

[Ref.: #42868]

Culture medium growth

[Ref.: #42868]

Culture medium link

Medium recipe at MediaDive

[Ref.: #42868]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #42868]	growth	40 °C
[Ref.: #43213]	maximum	50 °C
[Ref.: #43213]	minimum	25 °C
[Ref.: #43213]	optimum	42 °C
[Ref.: #43213]	growth	20-50 °C

[Ref.: #42868]	Temperature range	thermophilic
[Ref.: #43213]	Temperature range	thermophilic
[Ref.: #43213]	Temperature range	mesophilic
[Ref.: #43213]	Temperature range	thermophilic

		pH
[Ref.: #43213]	maximum	8.8
[Ref.: #43213]	minimum	7
[Ref.: #43213]	optimum	7.4-7.9
[Ref.: #43213]	growth	7.0-8.8

Information on physiology and metabolism Physiology and metabolism

[Ref.: #43213]

Oxygen tolerance

obligate anaerobe

[Ref.: #43213]

Nutrition type

organotroph

[Ref.: #43213]

Ability of spore formation

yes

[Ref.: #43213]

Halophily / tolerance level

moderately halophilic

	Salt		Salt conc.
[Ref.: #43213]		growth	100	g/L
[Ref.: #43213]	NaCl	optimum	20	g/L
[Ref.: #43213]	NaCl	minimum	0.5	%(w/v)
[Ref.: #43213]	NaCl	optimum	2	%(w/v)
[Ref.: #43213]	NaCl	maximum	10	%(w/v)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43213]	acetate ChEBI	-	growth
[Ref.: #43213]	arabinose ChEBI	-	growth
[Ref.: #43213]	arabinoxylan ChEBI	-	growth
[Ref.: #43213]	arsenate ChEBI	-	electron acceptor
[Ref.: #43213]	carboxymethylcellulose ChEBI	+	fermentation
[Ref.: #43213]	casein hydrolysate	-	growth
[Ref.: #43213]	cellobiose ChEBI	+	fermentation
[Ref.: #43213]	cellulose ChEBI	+	fermentation
[Ref.: #43213]	chitin ChEBI	+	fermentation
[Ref.: #43213]	dihydrogen ChEBI	-	growth
[Ref.: #43213]	elemental sulfur ChEBI	-	electron acceptor
[Ref.: #43213]	ethanol ChEBI	-	growth
[Ref.: #43213]	fructose ChEBI	+	fermentation
[Ref.: #43213]	fumarate ChEBI	-	growth
[Ref.: #43213]	fumarate ChEBI	-	electron acceptor
[Ref.: #43213]	galactose ChEBI	-	growth
[Ref.: #43213]	gelatin ChEBI	-	growth
[Ref.: #43213]	glucose ChEBI	-	growth
[Ref.: #43213]	glycerol ChEBI	-	growth
[Ref.: #43213]	lactate ChEBI	-	growth
[Ref.: #43213]	lactose ChEBI	-	growth
[Ref.: #43213]	maltose ChEBI	-	growth
[Ref.: #43213]	mannitol ChEBI	-	growth
[Ref.: #43213]	methanol ChEBI	-	growth
[Ref.: #43213]	nitrate ChEBI	-	electron acceptor
[Ref.: #43213]	nitrite ChEBI	-	electron acceptor
[Ref.: #43213]	pectin ChEBI	-	growth
[Ref.: #43213]	peptone	+	fermentation
[Ref.: #43213]	propionate ChEBI	-	growth
[Ref.: #43213]	pyruvate ChEBI	-	growth
[Ref.: #43213]	starch ChEBI	+	fermentation
[Ref.: #43213]	sucrose ChEBI	-	growth
[Ref.: #43213]	sulfate ChEBI	-	electron acceptor
[Ref.: #43213]	sulfite ChEBI	-	electron acceptor
[Ref.: #43213]	thiosulfate ChEBI	-	electron acceptor
[Ref.: #43213]	tryptone	-	growth
[Ref.: #43213]	xanthan ChEBI	+	fermentation
[Ref.: #43213]	xylan ChEBI	-	growth
[Ref.: #43213]	xylose ChEBI	-	growth
[Ref.: #43213]	yeast extract	+	fermentation
	Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #42868]	Sample type/isolated from	microbial mat developed under the flow of subsurface water in a gold mine at a depth of 3.54 km below the surface
[Ref.: #42868]	Geographic location (country and/or sea, region)	northern margin of the Witwatersrand Basin, near Carletonville, TauTona gold mine at a depth of 3.54 km below the surface
[Ref.: #42868]	Country	South Africa
[Ref.: #42868]	Country ISO 3 Code	ZAF
[Ref.: #42868]	Continent	Africa
[Ref.: #42868]	Geographic location	-26.4161°/27.4275°

[Ref.: #43213]	Sample type/isolated from	microbial mat of TauTona gold mine
[Ref.: #43213]	Sampling date	2013-04-17
[Ref.: #43213]	Geographic location (country and/or sea, region)	TauTona gold mine located at the northern margin of the Witwatersrand Basin, near Carletonville, South Africa
[Ref.: #43213]	Continent	Africa
[Ref.: #43213]	Geographic location	-26.0000°/27.0000°
* marker position based on {}

Isolation sources categories

#Engineered	#Other	#Mine
#Environmental	#Aquatic	-
#Environmental	#Microbial community	#Microbial mat

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence KX371568 (>99% sequence identity) for Sporosalibacterium tautonense subclade from Microbeatlas

Aquatic Samples	96
Soil Samples	20
Animal Samples	63
Plant Samples	3
Total Samples	182

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #42868]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #42868]	Sporosalibacterium tautonense strain MRo-4 16S ribosomal RNA gene, partial sequence	KX371568	1520	ENA	1859103 tax ID

[Ref.: #43213]	GC-content	32.9 mol%	genome sequence analysis
[Ref.: #42868]	GC-content	32.9 mol%	sequence analysis

Availability in culture collections External links

[Ref.: #42868]

Culture collection no.

DSM 28179, VKM B-2948

[Ref.: #96567]

SI-ID 405281

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Sporosalibacterium tautonense sp. nov., a thermotolerant, halophilic, hydrolytic bacterium isolated from a gold mine, and emended description of the genus Sporosalibacterium.	Podosokorskaya OA, Merkel AY, Heerden EV, Cason ED, Kopitsyn DS, Vasilieva M, Bonch-Osmolovskaya EA, Kublanov IV	Int J Syst Evol Microbiol	10.1099/ijsem.0.001737	2017	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#42868

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 28179

#43213

Olga A. Podosokorskaya, Alexander Y. Merkel, Esta van Heerden, Errol D. Cason, Dmitry S. Kopitsyn, Maria Vasilieva, Elizaveta A. Bonch-Osmolovskaya and Ilya V. Kublanov: Sporosalibacterium tautonense sp. nov., a thermotolerant, halophilic, hydrolytic bacterium isolated from a gold mine, and emended description of the genus Sporosalibacterium. IJSEM 67: 1457 - 1461 2017 ( DOI 10.1099/ijsem.0.001737 , PubMed 27974092 )

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#96567

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID405281.1 )

* These data were automatically processed and therefore are not curated

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