Name: Marinococcus halotolerans DSM 16375
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 1370

Type strain:

Species: Marinococcus halotolerans

Culture col. no.: DSM 16375, CIP 108945, KCTC 19045, YIM 70157, NBRC 106070

Strain history: <- WJ Li, Yunnan Univ., China

NCBI tax ID(s): 1122203 (strain), 301092 (species)

SI-ID 133633

DSM 16375, KCTC 19045, CIP 108945

Special Collections

Section

Marinococcus halotolerans DSM 16375 is an aerobe, Gram-positive, motile bacterium that forms circular colonies and has a orange pigmentation.

coccus-shaped
colony-forming
Gram-positive
motile
pigmented
aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Marinococcus*
	Species	**Marinococcus halotolerans**
	Full Scientific Name (LPSN)	Marinococcus halotolerans Li et al. 2005

Information on morphological and physiological properties Morphology

[Ref.: #23006]	Gram stain	positive
[Ref.: #67771]	Gram stain	positive

[Ref.: #23006]	Cell length	1.0-1.2 µm

[Ref.: #23006]	Cell shape	coccus-shaped
[Ref.: #67771]	Cell shape	coccus-shaped
[Ref.: #67771]	Cell shape	sphere-shaped

[Ref.: #23006]	Motility	yes
[Ref.: #67771]	Motility	yes

[Ref.: #23006]	Flagellum arrangement	monotrichous, polar
[Ref.: #67771]	Flagellum arrangement	monotrichous, polar

[Ref.: #23006]	Colony size	1.5-1.8 mm
[Ref.: #23006]	Colony color	orange
[Ref.: #23006]	Colony shape	circular
[Ref.: #23006]	Incubation period	2 days

[Ref.: #23006]	Pigment production	yes
[Ref.: #23006]	Pigmentation color	orange

Information on culture and growth conditions Culture and growth conditions

[Ref.: #6412]

Culture medium

CORYNEBACTERIUM AGAR (DSMZ Medium 53)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

expand / minimize

[Ref.: #6412]

Culture medium

ISP 5 MEDIUM (DSMZ Medium 993)

[Ref.: #6412]

Culture medium growth

[Ref.: #6412]

Culture medium link

Medium recipe at MediaDive

[Ref.: #6412]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #6412]	growth	28 °C
[Ref.: #23006]	optimum	28.0 °C
[Ref.: #67771]	growth	28 °C

	pH	Kind of pH		pH
[Ref.: #23006]			optimum	7.0-7.5
[Ref.: #23006]			growth	6.5-9.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #23006]	Oxygen tolerance	aerobe
[Ref.: #67771]	Oxygen tolerance	aerobe

[Ref.: #23006]	Ability of spore formation	no
[Ref.: #67771]	Ability of spore formation	no
[Ref.: #69481]	Ability of spore formation	no Confidence in %96

	Salt		Salt conc.
[Ref.: #23006]	NaCl	growth	0.0-25.0	%
[Ref.: #23006]	KCl	growth	0.0-25.0	%
[Ref.: #23006]	NaCl	optimum	10.0	%(w/v)
[Ref.: #23006]	KCl	optimum	10.0	%(w/v)

[Ref.: #67771]

Observation

quinones: MK-7 and MK-6

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #23006]	acetamide ChEBI	+	carbon source
[Ref.: #23006]	arabinitol ChEBI	-	carbon source
[Ref.: #23006]	arabinose ChEBI	-	carbon source
[Ref.: #23006]	casein	-	hydrolysis
[Ref.: #23006]	cellobiose ChEBI	+	carbon source
[Ref.: #23006]	cellulose ChEBI	-	growth
[Ref.: #23006]	dextrin ChEBI	+	carbon source
[Ref.: #23006]	esculin ChEBI	+	builds acid from
[Ref.: #23006]	fructose ChEBI	+	carbon source
[Ref.: #23006]	galactose ChEBI	+	carbon source
[Ref.: #23006]	gelatin ChEBI	-	hydrolysis
[Ref.: #23006]	glucose ChEBI	-	fermentation
[Ref.: #23006]	glucose ChEBI	+	builds acid from
[Ref.: #23006]	glucose ChEBI	+	carbon source
[Ref.: #23006]	maltose ChEBI	+	carbon source
[Ref.: #23006]	mannitol ChEBI	+	builds acid from
[Ref.: #23006]	mannitol ChEBI	+	carbon source
[Ref.: #23006]	mannose ChEBI	+	carbon source
[Ref.: #23006]	myo-inositol ChEBI	-	carbon source
[Ref.: #23006]	nitrate ChEBI	+	reduction
[Ref.: #23006]	rhamnose ChEBI	-	carbon source
[Ref.: #23006]	ribitol ChEBI	-	carbon source
[Ref.: #23006]	salicin ChEBI	+	carbon source
[Ref.: #23006]	sorbitol ChEBI	-	carbon source
[Ref.: #23006]	starch ChEBI	-	degradation
[Ref.: #23006]	starch ChEBI	-	hydrolysis
[Ref.: #23006]	tween 20 ChEBI	-	degradation
[Ref.: #23006]	tween 80 ChEBI	-	degradation
[Ref.: #23006]	xylose ChEBI	+	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #23006]	acetoin ChEBI	no
[Ref.: #23006]	ammonium ChEBI	no
[Ref.: #23006]	hydrogen sulfide ChEBI	no
[Ref.: #23006]	melanin ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test	Methylred-test
[Ref.: #23006]		acetoin ChEBI	-
[Ref.: #23006]		glucose ChEBI		-

	Enzyme	Enzyme activity	EC number
[Ref.: #23006]	alpha-galactosidase	-	3.2.1.22
[Ref.: #23006]	arginine dihydrolase	-	3.5.3.6
[Ref.: #23006]	beta-galactosidase	+	3.2.1.23
[Ref.: #23006]	beta-glucosidase	+	3.2.1.21
[Ref.: #23006]	catalase	+	1.11.1.6
[Ref.: #23006]	cytochrome oxidase	-	1.9.3.1
[Ref.: #23006]	L-aspartate arylamidase	-	3.4.11.21
[Ref.: #23006]	lipase	+
[Ref.: #23006]	lysine decarboxylase	-	4.1.1.18
[Ref.: #23006]	ornithine decarboxylase	-	4.1.1.17

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #23006]	Sample type/isolated from	hypersaline soil sample
[Ref.: #23006]	Geographic location (country and/or sea, region)	Qinghai
[Ref.: #23006]	Country	China
[Ref.: #23006]	Country ISO 3 Code	CHN
[Ref.: #23006]	Continent	Asia
[Ref.: #23006]	Enrichment culture	Modified medium SG
[Ref.: #23006]	Enrichment culture composition	containing (g l-1): Casamino acids (7.5), yeast extract (10.0),trisodium citrate (3.0), NaCl (2.0), KCl (2.0), MgCl2.6H2O (250), MgSO4.7H2O (1.0), FeSO4.7H2O (0.05), MnSO4.7H2O (0.0002)
[Ref.: #23006]	Enrichment culture duration	14 days
[Ref.: #23006]	Enrichment culture temperature	28.0 ̊C

[Ref.: #6412]	Sample type/isolated from	soil
[Ref.: #6412]	Geographic location (country and/or sea, region)	Qinghai Province
[Ref.: #6412]	Country	China
[Ref.: #6412]	Country ISO 3 Code	CHN
[Ref.: #6412]	Continent	Asia

[Ref.: #67771]	Sample type/isolated from	From saline soil
[Ref.: #67771]	Geographic location (country and/or sea, region)	Qinghai
[Ref.: #67771]	Country	China
[Ref.: #67771]	Country ISO 3 Code	CHN
[Ref.: #67771]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental	#Terrestrial	#Soil
#Condition	#Saline	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AB769482 (>99% sequence identity) for Marinococcus from Microbeatlas

Aquatic Samples	1141
Soil Samples	176
Animal Samples	1431
Plant Samples	69
Total Samples	2817

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #6412]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #6412]	Marinococcus halotolerans strain YIM 70157 16S ribosomal RNA gene, partial sequence	AY817493	1454	GenBank ENA	301092 tax ID
[Ref.: #20218]	Marinococcus halotolerans gene for 16S ribosomal RNA, partial sequence, strain: KCTC 19045	AB769482	1491	GenBank ENA	301092 tax ID	*
[Ref.: #124043]	Marinococcus halotolerans gene for 16S rRNA, partial sequence, strain: NBRC 106070.	AB682353	1498	GenBank ENA	301092 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Marinococcus halotolerans DSM 16375	GCA_000420725	contig	GenBank ENA	1122203 tax ID	*
[Ref.: #66792]	Marinococcus halotolerans DSM 16375	1122203.4	wgs	PATRIC	1122203 tax ID	*
[Ref.: #66792]	Marinococcus halotolerans DSM 16375	2524614596	draft	JGI IMG/M	1122203 tax ID	*

[Ref.: #6412]	GC-content	48.5 mol%
[Ref.: #23006]	GC-content	48.5 mol%	high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Marinococcus halotolerans DSM 16375 PATRIC: 1122203.4
[Ref.: #69481]	spore-forming	spore-formingⓘ	no	96	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Marinococcus halotolerans DSM 16375 PATRIC: 1122203.4
[Ref.: #69480]	gram-positive	gram-positiveⓘ	yes	81.858	yes
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	96.069	yes
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	66.079	yes
[Ref.: #69480]	aerobic	aerobicⓘ	yes	80.667	no
[Ref.: #69480]	thermophilic	thermophileⓘ	no	87.957	no
[Ref.: #69480]	flagellated	motile2+ⓘ	yes	78.587	yes

Availability in culture collections External links

[Ref.: #6412]

Culture collection no.

DSM 16375, CIP 108945, KCTC 19045, YIM 70157, NBRC 106070

[Ref.: #71021]

SI-ID 133633

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Marinococcus salis sp., nov., a moderately halophilic bacterium isolated from a salt marsh.	Vishnuvardhan Reddy S, Thirumala M, Farooq M, Sasikala C, Venkata Ramana C	Arch Microbiol	10.1007/s00203-016-1263-z	2016	*
Phylogeny	Marinococcus tarijensis sp. nov., a moderately halophilic bacterium isolated from a salt mine.	Balderrama-Subieta A, Guzman D, Minegishi H, Echigo A, Shimane Y, Hatada Y, Quillaguaman J	Int J Syst Evol Microbiol	10.1099/ijs.0.045401-0	2013	*
Phylogeny	Marinococcus luteus sp. nov., a halotolerant bacterium isolated from a salt lake, and emended description of the genus Marinococcus.	Wang Y, Cao LL, Tang SK, Lou K, Mao PH, Jin X, Jiang CL, Xu LH, Li WJ	Int J Syst Evol Microbiol	10.1099/ijs.0.009670-0	2009	*
Phylogeny	Marinococcus halotolerans sp. nov., isolated from Qinghai, north-west China.	Li WJ, Schumann P, Zhang YQ, Chen GZ, Tian XP, Xu LH, Stackebrandt E, Jiang CL	Int J Syst Evol Microbiol	10.1099/ijs.0.63596-0	2005	*

References

#6412

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 16375

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#23006

Wen-Jun Li,Peter Schumann,Yu-Qin Zhang,Guo-Zhong Chen,Xin-Peng Tian,Li-Hua Xu,Erko Stackebrandt,Cheng-Lin Jiang: Marinococcus halotolerans sp. nov., isolated from Qinghai, north-west China. IJSEM 55: 1801 - 1804 2005 ( DOI 10.1099/ijs.0.63596-0 , PubMed 16166669 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#71021

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID133633.1 )

#124043

Dr. Isabel Schober, Dr. Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .

* These data were automatically processed and therefore are not curated

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