Saccharothrix mutabilis subsp. mutabilis (DSM 43853, ATCC 31520, IFO 14310)

Name: Saccharothrix mutabilis subsp. mutabilis (DSM 43853, ATCC 31520, IFO 14310)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 13478

Type strain

Strain Designation AAA-0025

Culture col. no. DSM 43853 ATCC 31520 IFO 14310 JCM 3380 NBRC 14310 8 more

NCBI tax ID(s) 33921 66855

Special Collections DSMZ JCM KCTC Wink compendium

History <- ATCC <- Smith, Kline and French Labs, AAA-0025 IFM 0240 <-- IFO 14310 <-- ATCC 31520 <-- Smith Kline & French Labs.; AAA-025.

Links

Saccharothrix mutabilis subsp. mutabilis AAA-0025 is a spore-forming, mesophilic prokaryote that builds an aerial mycelium and was isolated from soil.

spore-forming mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 92835

ATCC 31520,DSM 43853,IFO 14310,NRRL B-16077,JCM 3380,CCRC 12528,VKM Ac-2023,AS 4.1354,KCTC 9397,NBRC 14310,BCRC 12528,CGMCC 4.1354

@ref 20215

Last LPSN update 2026-02-09 11:21:09

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Pseudonocardiales

Family Pseudonocardiaceae

Genus Saccharothrix

Species Saccharothrix mutabilis subsp. mutabilis

Full scientific name Saccharothrix mutabilis subsp. mutabilis (Shearer et al. 1983) Grund and Kroppenstedt 1990

BacDive ID	Other strains from **Saccharothrix mutabilis subsp. mutabilis** (1)	Type strain
13477	S. mutabilis subsp. mutabilis N838, DSM 43386, IMRU 1593, CDC W2104

Morphology

Colony morphology

@ref	Colony color	Incubation period	Medium used
19968	Orange	10-14 days	ISP 2
19968	Yellow	10-14 days	ISP 3
19968	Yellow	10-14 days	ISP 4
19968	Yellow	10-14 days	ISP 5
19968	Bright yellow	10-14 days	ISP 6
19968	Orange	10-14 days	ISP 7

Multicellular morphology

@ref	Complex name	Complex color	Medium name
19968	Aerial Mycelium	White	ISP 2
19968	Aerial Mycelium	White	ISP 3
19968	Aerial Mycelium	White	ISP 4
19968	Aerial Mycelium	White	ISP 5
19968			ISP 6
19968	Aerial Mycelium	White	ISP 7

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
11335	GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)	Medium recipe at MediaDive	Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
19968	ISP 2		Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19968	ISP 3		Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19968	ISP 4		Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19968	ISP 5		Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19968	ISP 6		Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19968	ISP 7		Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
11335	positive	growth	28	mesophilic
19968	positive	optimum	25	mesophilic
67770	positive	growth	28	mesophilic

Physiology and metabolism

Spore formation

@ref	Spore description	Spore formation
19968	Formation of spore chains (rectiflixibilis), spore surface smooth

Compound production

@ref	Compound
11335	polynitrosin
20216	Polynitroxin
19968	Tetrazomine
67770	Polynitroxin

Halophily

@ref	Salt	Growth	Tested relation	Concentration
19968	NaCl	positive	maximum	5 %

Observation

67770

Observationquinones: MK-9(H₄)

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
19968	22599 ChEBI	arabinose	+
68368	29016 ChEBI	arginine	+	hydrolysis	from API 20E
19968	62968 ChEBI	cellulose	-
68368	16947 ChEBI	citrate	+	assimilation	from API 20E
19968	28757 ChEBI	fructose	+
68368	5291 ChEBI	gelatin	+	hydrolysis	from API 20E
19968	17234 ChEBI	glucose	+
68368	25094 ChEBI	lysine	+	degradation	from API 20E
19968	29864 ChEBI	mannitol	-
19968	17268 ChEBI	myo-inositol	+
68368	18257 ChEBI	ornithine	+	degradation	from API 20E
19968	16634 ChEBI	raffinose	-
19968	26546 ChEBI	rhamnose	-
19968	17992 ChEBI	sucrose	-
68368	27897 ChEBI	tryptophan	-	energy source	from API 20E
68368	16199 ChEBI	urea	+	hydrolysis	from API 20E
19968	18222 ChEBI	xylose	-

Metabolite production

@ref	Chebi-ID	Metabolite
68368	15688 ChEBI	acetoin	from API 20E
68368	16136 ChEBI	hydrogen sulfide	from API 20E
68368	35581 ChEBI	indole	from API 20E

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Indole test
68368	15688 ChEBI	acetoin	+		from API 20E
68368	35581 ChEBI	indole		-	from API 20E

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68368	arginine dihydrolase	+	3.5.3.6	from API 20E
68382	beta-galactosidase	+	3.2.1.23	from API zym
68368	beta-galactosidase	+	3.2.1.23	from API 20E
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
68382	cystine arylamidase	-	3.4.11.3	from API zym
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
68368	gelatinase	+		from API 20E
68382	leucine arylamidase	-	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
68368	lysine decarboxylase	+	4.1.1.18	from API 20E
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
68368	ornithine decarboxylase	+	4.1.1.17	from API 20E
68382	trypsin	+	3.4.21.4	from API zym
68368	tryptophan deaminase	-	4.1.99.1	from API 20E
68368	urease	+	3.5.1.5	from API 20E
68382	valine arylamidase	-		from API zym

API 20E

@ref	ONPG	ADH (Arg)	LDC (Lys)	ODC	CIT	H2S productionH2S	URE	TDA (Trp)	IND	Acetoin production (Voges Proskauer test)VP	GEL	GLU	MAN	INO	Sor	RHA	SAC	MEL	AMY	ARA	OX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	OF-O	OF-F
19968	+	+	+	+	+	-	+	-	-	+	+	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
19968	not determinedn.d.	+	+	+	-	-	-	-	+	-	+	+	-	+	-	+	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Soil

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
11335	soil
67770	Soil	Gujarat	India	IND	Asia

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
11335	1	Risk group (German classification) According to TRBA 466
19968	1	German classification According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	NCBI tax ID	Score
124043	ASM3952114v1 assembly for Saccharothrix mutabilis subsp. mutabilis JCM 3380	scaffold	GCA_039521145	66855	58.74

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
20218	S.mutabilis (ssp. mutabilis) DSM 43853 16S ribosomal RNA (partial)	X76966	1458		33921
124043	Saccharothrix mutabilis subsp. mutabilis strain JCM 3380 16S ribosomal RNA gene, partial sequence.	MT760461	1335		66855

GC content

@ref	GC-content (mol%)	Method
67770	73.1	thermal denaturation, midpoint method (Tm)

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
Metabolism	Dual-Mechanism Confers Self-Resistance to the Antituberculosis Antibiotic Capreomycin.	Pan YC, Wang YL, Toh SI, Hsu NS, Lin KH, Xu Z, Huang SC, Wu TK, Li TL, Chang CY.	ACS Chem Biol	10.1021/acschembio.1c00799	2022
	Newly Discovered Mechanisms of Antibiotic Self-Resistance with Multiple Enzymes Acting at Different Locations and Stages.	Chen X, Pan HX, Tang GL.	Antibiotics (Basel)	10.3390/antibiotics12010035	2022
	Discovery and characterization of genes conferring natural resistance to the antituberculosis antibiotic capreomycin.	Toh SI, Elaine Keisha J, Wang YL, Pan YC, Jhu YH, Hsiao PY, Liao WT, Chen PY, Ko TM, Chang CY.	Commun Biol	10.1038/s42003-023-05681-6	2023
Metabolism	Detection of antibiotics synthetized in microfluidic picolitre-droplets by various actinobacteria.	Mahler L, Wink K, Beulig RJ, Scherlach K, Tovar M, Zang E, Martin K, Hertweck C, Belder D, Roth M.	Sci Rep	10.1038/s41598-018-31263-2	2018
Enzymology	Fluvirucins A1, A2, B1, B2, B3, B4 and B5, new antibiotics active against influenza A virus. IV. Taxonomy on the producing organisms.	Tomita K, Oda N, Hoshino Y, Ohkusa N, Chikazawa H.	J Antibiot (Tokyo)	10.7164/antibiotics.44.940	1991

References

#11335	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 43853
#19968	Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20216	Curators of the HKI: Collection Description Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut (HKI) . Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut (HKI):
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68368	Automatically annotated from API 20E .
#68382	Automatically annotated from API zym .
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Saccharothrix mutabilis subsp. mutabilis (DSM 43853, ATCC 31520, IFO 14310)

Name and taxonomic classification

Morphology

Colony morphology

Multicellular morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Spore formation

Compound production

Halophily

Observation

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API 20E

API zym

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_039521145

16S sequences

GC content

Genome-based predictions

Literature

Literature

References

BacDive

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