Saccharothrix longispora (DSM 43749, ATCC 35109, INA 10222)

Name: Saccharothrix longispora (DSM 43749, ATCC 35109, INA 10222)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 13476

Type strain

Culture col. no. DSM 43749 ATCC 35109 INA 10222 VKM Ac-907 NRRL B-16116 9 more

NCBI tax ID(s) 33920

Special Collections DSMZ JCM KCTC Wink compendium

History <- N.S. Agre, INA KCC A-0314 <-- IMET 9603 <-- INA 10222.

Links

Saccharothrix longispora DSM 43749 is a mesophilic prokaryote that was isolated from soil.

mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 46775

ATCC 35109,DSM 43749,JCM 3314,CCRC 13395,HUT 6594,IFO 14522,IMET 9603,NRRL B-16116,VKM Ac-907,AS 4.1357,KCTC 9394,NBRC 14522,BCRC 13395,CGMCC 4.1357

@ref 20215

Last LPSN update 2026-02-09 11:21:09

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Pseudonocardiales

Family Pseudonocardiaceae

Genus Saccharothrix

Species Saccharothrix longispora

Full scientific name Saccharothrix longispora (Preobrazhenskaya and Sveshnikova 1974) Grund and Kroppenstedt 1990

Synonyms (2)

Nocardiopsis longispora
Actinomadura longispora

Morphology

Cell morphology

@ref	Gram stain	Confidence
125439	positive	99.9

Colony morphology

@ref	Colony color	Incubation period	Medium used
19583	Yellow orange	10-14 days	ISP 2
19583	Yellow orange	10-14 days	ISP 3
19583	Yellow orange	10-14 days	ISP 4
19583	Yellow orange	10-14 days	ISP 5
19583	Yellow orange	10-14 days	ISP 6
19583	Yellow orange	10-14 days	ISP 7

Multicellular morphology

@ref	Forms multicellular complex	Medium name
19583		ISP 2
19583		ISP 3
19583		ISP 4
19583		ISP 5
19583		ISP 6
19583		ISP 7

Multimedia

@ref:	11246
multimedia content:	DSM_43749.jpg
multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_43749.jpg
caption:	Medium 65 28°C
intellectual property rights:	© Leibniz-Institut DSMZ
manual_annotation:	1

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
11246	GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)	Medium recipe at MediaDive	Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
19583	ISP 2		Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19583	ISP 3		Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19583	ISP 4		Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19583	ISP 5		Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19583	ISP 6		Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19583	ISP 7		Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
11246	positive	growth	28	mesophilic
19583	positive	optimum	28	mesophilic
67770	positive	growth	28	mesophilic

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
125439	obligate aerobe	99.2

Spore formation

@ref	Spore formation	Confidence
125439		90

Halophily

@ref	Salt	Growth	Tested relation	Concentration
19583	NaCl	positive	maximum	5 %

Observation

67770

Observationquinones: MK-9(H₄)

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
19583	22599 ChEBI	arabinose	+
68368	29016 ChEBI	arginine	+	hydrolysis	from API 20E
19583	62968 ChEBI	cellulose	+/-
68368	16947 ChEBI	citrate	+	assimilation	from API 20E
19583	28757 ChEBI	fructose	+
68368	5291 ChEBI	gelatin	+	hydrolysis	from API 20E
19583	17234 ChEBI	glucose	+
68368	25094 ChEBI	lysine	+	degradation	from API 20E
19583	29864 ChEBI	mannitol	+
19583	17268 ChEBI	myo-inositol	+
68368	18257 ChEBI	ornithine	+	degradation	from API 20E
19583	16634 ChEBI	raffinose	+/-
19583	26546 ChEBI	rhamnose	+/-
19583	17992 ChEBI	sucrose	+
68368	27897 ChEBI	tryptophan	-	energy source	from API 20E
68368	16199 ChEBI	urea	+	hydrolysis	from API 20E
19583	18222 ChEBI	xylose	-

Metabolite production

@ref	Chebi-ID	Metabolite
68368	15688 ChEBI	acetoin	from API 20E
68368	16136 ChEBI	hydrogen sulfide	from API 20E
68368	35581 ChEBI	indole	from API 20E

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Indole test
68368	15688 ChEBI	acetoin	+		from API 20E
68368	35581 ChEBI	indole		-	from API 20E

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	+	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68368	arginine dihydrolase	+	3.5.3.6	from API 20E
68382	beta-galactosidase	+	3.2.1.23	from API zym
68368	beta-galactosidase	-	3.2.1.23	from API 20E
68382	beta-glucosidase	+	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
68382	cystine arylamidase	-	3.4.11.3	from API zym
68382	esterase (C 4)	-		from API zym
68382	esterase lipase (C 8)	+		from API zym
68368	gelatinase	+		from API 20E
68382	leucine arylamidase	-	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
68368	lysine decarboxylase	+	4.1.1.18	from API 20E
68382	N-acetyl-beta-glucosaminidase	+	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
68368	ornithine decarboxylase	+	4.1.1.17	from API 20E
68382	trypsin	+	3.4.21.4	from API zym
68368	tryptophan deaminase	-	4.1.99.1	from API 20E
68368	urease	+	3.5.1.5	from API 20E
68382	valine arylamidase	-		from API zym

API 20E

@ref	ONPG	ADH (Arg)	LDC (Lys)	ODC	CIT	H2S productionH2S	URE	TDA (Trp)	IND	Acetoin production (Voges Proskauer test)VP	GEL	GLU	MAN	INO	Sor	RHA	SAC	MEL	AMY	ARA	OX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	OF-O	OF-F
19583	-	+	+	+	+	-	+	-	-	+	+	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
19583	not determinedn.d.	+	-	+	-	-	-	-	+	-	+	+	+	+	-	+	+	+	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Soil

Isolation

@ref	Sample type	Country	Country ISO 3 Code	Continent
11246	soil	India	IND	Asia
67770	Soil

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
11246	1	Risk group (German classification) According to TRBA 466
19583	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
124043	ASM3145522v1 assembly for Saccharothrix longispora DSM 43749	contig	GCA_031455225	33920.4	2919027109	33920	78.09
124043	ASM3953537v1 assembly for Saccharothrix longispora JCM 3314	contig	GCA_039535375			33920	76.58
124043	ASM4242856v1 assembly for Saccharothrix longispora JCM 3314	contig	GCA_042428565			33920	68.17

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	S.longispora (DSM 43749) 16S ribosomal RNA (partial)	X76964	1459	33920
20218	Saccharothrix longispora strain NRRL B-16116 16S ribosomal RNA gene, partial sequence	AF114809	1456	33920
124043	Saccharothrix longispora strain JCM 3314 16S ribosomal RNA gene, partial sequence.	MT760446	1329	33920

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Saccharothrix longispora DSM 43749
IMG: 2919027109

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	yes	90.00	no
125439	motility	BacteriaNetⓘ	no	89.70	no
125439	gram_stain	BacteriaNetⓘ	positive	99.90	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	99.20	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Biocontrol of toxinogenic Aspergillus flavus and Fusarium oxysporum f. sp. albedinis by two rare Saharan actinomycetes strains and LC-ESI/MS-MS profiling of their antimicrobial products.	Meliani H, Makhloufi A, Cherif A, Mahjoubi M, Makhloufi K.	Saudi J Biol Sci	10.1016/j.sjbs.2022.103288	2022
Phylogeny	Saccharothrix sp. PAL54, a new chloramphenicol-producing strain isolated from a Saharan soil.	Aouiche A, Sabaou N, Meklat A, Zitouni A, Bijani C, Mathieu F, Lebrihi A	World J Microbiol Biotechnol	10.1007/s11274-011-0892-2	2011
Phylogeny	Whole-Genome-Based Reassessment of Saccharothrix Phylogeny, with the Proposal of Two New Subspecies: Saccharothrix longispora subsp. Longispora subsp. nov. and Saccharothrix longispora subsp. Yanglingensis subsp. nov., comb. nov.	Dif G, Djemouai N, Bouras N, Meklat A, Zitouni A.	Curr Microbiol	10.1007/s00284-025-04361-4	2025
Phylogeny	Saccharothrix lopnurensis sp. nov., a filamentous actinomycete isolated from sediment of Lop Nur.	Li YQ, Liu L, Cheng C, Shi XH, Lu CY, Dong ZY, Salam N, An DD, Li WJ.	Antonie Van Leeuwenhoek	10.1007/s10482-015-0550-z	2015
Phylogeny	Saccharothrix hoggarensis sp. nov., an actinomycete isolated from Saharan soil.	Boubetra D, Zitouni A, Bouras N, Mathieu F, Lebrihi A, Schumann P, Sproer C, Klenk HP, Sabaou N.	Int J Syst Evol Microbiol	10.1099/ijs.0.039099-0	2013
Phylogeny	Saccharothrix isguenensis sp. nov., an actinobacterium isolated from desert soil.	Bouznada K, Bouras N, Mokrane S, Chaabane Chaouch F, Zitouni A, Potter G, Sproer C, Klenk HP, Sabaou N	Int J Syst Evol Microbiol	10.1099/ijsem.0.001430	2016
Phylogeny	Saccharothrix yanglingensis sp. nov., an antagonistic endophytic actinomycete isolated from cucumber plant.	Yan X, Huang LL, Tu X, Gao XN, Kang ZS	Antonie Van Leeuwenhoek	10.1007/s10482-011-9631-9	2011

References

#11246	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 43749
#19583	Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68368	Automatically annotated from API 20E .
#68382	Automatically annotated from API zym .
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Saccharothrix longispora (DSM 43749, ATCC 35109, INA 10222)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Multicellular morphology

Multimedia

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Observation

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API 20E

API zym

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_031455225

16S sequences

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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