Alcaligenes aquatilis (CCUG 50924, CIP 108999, LMG 22996)

Name: Alcaligenes aquatilis (CCUG 50924, CIP 108999, LMG 22996)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 134159

Type strain

Culture col. no. CCUG 50924 CIP 108999 LMG 22996

NCBI tax ID(s) 323284

Special Collections CCUG CIP Literature strains

History CIP <- 2005, CCUG <- 2005, LMG

Links

Alcaligenes aquatilis CCUG 50924 is an anaerobe, Gram-negative, motile bacterium that was isolated from Sediment,estuary.

Gram-negative motile rod-shaped anaerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 127553

LMG 22996,CCUG 50924,CIP 108999

@ref 20215

Last LPSN update 2026-05-29 08:38:16

Domain Bacteria

Phylum Pseudomonadota

Class Betaproteobacteria

Order Burkholderiales

Family Alcaligenaceae

Genus Alcaligenes

Species Alcaligenes aquatilis

Full scientific name Alcaligenes aquatilis Van Trappen et al. 2005

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Confidence
31582	negative	1.75 µm	0.9 µm	rod-shaped
116439	negative			rod-shaped
125439	negative				99.581

Pigmentation

31582

Productionyes

Culture and growth conditions

Culture medium

@ref	Name	Growth	Composition	Medium link
36307	MEDIUM 328- for nutrient agar		Distilled water make up to (1000.000 ml);Agar (15.000 g);Peptone (5.000g);Beef extract (3.000 g)
116439	CIP Medium 328			Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
31582	positive	growth	04-35
31582	positive	optimum	21
36307	positive	growth	30
59049	positive	growth	28-37

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance
31582	anaerobe
59049	aerobe
116439	obligate aerobe

Spore formation

@ref	Spore formation	Confidence
125439		99.976

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
116439	17632 ChEBI	nitrate	-	reduction
116439	16301 ChEBI	nitrite	+	reduction

Metabolite production

@ref	Chebi-ID	Metabolite	Production
116439	35581 ChEBI	indole

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
116439	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	+	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	+	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	+	3.2.1.24	from API zym
68382	beta-galactosidase	+	3.2.1.23	from API zym
68382	beta-glucosidase	+	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
31582	catalase	+	1.11.1.6
116439	catalase	+	1.11.1.6
68382	cystine arylamidase	+	3.4.11.3	from API zym
31582	cytochrome oxidase	+	1.9.3.1
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
116439	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
116439	ornithine decarboxylase	-	4.1.1.17
116439	oxidase	+
68382	trypsin	+	3.4.21.4	from API zym
116439	urease	-	3.5.1.5
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
116439	-	+	+	+	-	+	-	+	+	+	+	+	+	+	-	+	+	-	+	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Estuary
#Environmental	#Aquatic	#Sediment

Isolation

@ref	Sample type	Sampling date	Geographic location	Country	Country ISO 3 Code	Continent
59049	Sediment,estuary	1983	Weser	Germany	DEU	Europe
116439	Environment, Sediments		Weser estuary	Germany	DEU	Europe

Taxonmaps

69479

Global distribution of 16S sequence AJ937889 (>99% sequence identity) for Alcaligenes from Microbeatlas

Aquatic Samples	1182
Soil Samples	516
Animal Samples	2918
Plant Samples	286
Total Samples	4902

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
116439	1	Risk group (French classification)

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	NCBI tax ID	Score
124043	ASM4264964v1 assembly for Alcaligenes aquatilis CCUG 50924	contig	GCA_042649645	323284	58.34

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
31582	Alcaligenes aquatilis 16S rRNA gene, type strain LMG 22996T	AJ937889	1082	323284
124043	Alcaligenes aquatilis strain LMG 22996 16S ribosomal RNA gene, partial sequence.	OR801642	1491	323284
124043	Alcaligenes aquatilis strain LMG 22996 16S ribosomal RNA gene, partial sequence.	JX986974	1491	323284
124043	Alcaligenes aquatilis strain LMG 22996 16S ribosomal RNA gene, partial sequence.	KX345927	917	323284
124043	Alcaligenes aquatilis strain LMG 22996 16S ribosomal RNA gene, partial sequence.	KX345319	912	323284

GC content

31582

GC-content (mol%)56

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM4264964v1 assembly for Alcaligenes aquatilis CCUG 50924
NCBI: GCA_042649645

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	gram_stain	BacteriaNetⓘ	negative	99.58	no
125439	oxygen_tolerance	BacteriaNetⓘ	facultative anaerobe	89.99	no
125439	motility	BacteriaNetⓘ	yes	89.82	no
125439	spore_formation	BacteriaNetⓘ	no	99.98	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Enzymology	Isolation and Characterization of Highly Active Uricase from Alcaligenes spp. Strain UR1.	Alshareef A, El-Readi MZ, Neyaz LA, Abulreesh HH, Alsaigh AA, Khalel AF, Alshehri WA, Elbanna K.	Pol J Microbiol	10.33073/pjm-2025-009	2025
	Antimicrobial Activity of Some Refractory Ceramic Bodies (RCBs) Prepared from Petroleum Waste Sludge (PWS) and Local Bauxite Mineral.	Saddiq AA, Baghdadi AM, Algamal Y.	ACS Omega	10.1021/acsomega.4c09076	2025
Phylogeny	Symbiotic and Nonsymbiotic Bacteria Associated With the Entomo-Pathogenic Nematode, Heterorhabditis spp (Rhabditida: Heterorhabditidae) From South India.	Menon AG, Bhaskar H, Gopal KS, M R, Subramanian SM.	J Basic Microbiol	10.1002/jobm.202400108	2024
	Optimization of fermentation conditions for enhanced L-arginase production by Alcaligenes aquatilis BC2 using response surface methodology	Assega B, Getahun K, Jiru T, Yohannes T, Aemero M, Andualem B.	J Genet Eng Biotechnol		2025
	Extracellular biosynthesis of CuO-TiO₂ nanocomposites using Alcaligenes aquatilis for the photodegradation of reactive and azo dyes under visible light irradiation.	Agarwalla S, Shetty Kodialbail V.	Environ Sci Pollut Res Int	10.1007/s11356-023-28489-7	2025
Phylogeny	Production of extracellular L-arginase by Alcaligenes aquatilis BC2 isolated from soda lakes (Lake Chitu) of Ethiopia.	Assega BG, Getahun KA, Milkessa T, Yohannes TG, Moges F, Aemero M, Andualem B.	J Ind Microbiol Biotechnol	10.1093/jimb/kuaf017	2024
	Long-term stability of reactor microbiome through bioaugmentation with Alcaligenes aquatilis AS1 promotes nitrogen removal of piggery wastewater.	Xianhe Cao, Hu C, Sun X, Zhang L, Wang H, Dong L, Li S.	J Environ Manage	10.1016/j.jenvman.2022.117146	2023
	Characterization of Alcaligenes aquatilis as a novel member of heterotrophic nitrifier-aerobic denitrifier and its performance in treating piggery wastewater.	Cao X, Zhao B, Wu Y, Huang J, Wang H, Sun X, Li S.	Bioresour Technol	10.1016/j.biortech.2022.127176	2022
	Dirammox-dominated microbial community for biological nitrogen removal from wastewater.	Hu Y, Wang Y, Wang R, Wang X, Liu SJ.	Appl Microbiol Biotechnol	10.1007/s00253-024-13214-2	2024
	Microbiologically influenced corrosion of Cu by marine ammonifying Alcaligenes aquatilis bacterium.	Guo Z, Ruan Q, Liu T, Mao X, Chai Z, Guo N, Dong L.	Bioelectrochemistry	10.1016/j.bioelechem.2022.108052	2022
	Antimicrobial Activity of Nanozirconium Oxide.	Bahammam HA, Bahammam LA, Baghdadi AM, Saddiq A, Algamal Y.	ACS Omega	10.1021/acsomega.3c08580	2024
	Characterization of biofilm formation and reduction of hexavalent chromium by bacteria isolated from tannery sludge.	Maurya A, Kumar PS, Raj A.	Chemosphere	10.1016/j.chemosphere.2021.131795	2022
	Lab-scale autothermal thermophilic aerobic digestion can maintain and remove nitrogen by controlling shear stress and oxygen supply system.	Zhang M, Tashiro Y, Asakura Y, Ishida N, Watanabe K, Yue S, Akiko MN, Sakai K.	J Biosci Bioeng	10.1016/j.jbiosc.2021.05.008	2021
	Inhibition of RNase to Attenuate Fungal-Manipulated Rhizosphere Microbiome and Diseases.	Yang B, Yang S, Wang X, Zhang Y, Zhao Y, Tao M, Zhu J, Zhang W, Wang Y, Duan K, Wang Y, Ye W, Guo Z, Wang Y.	Adv Sci (Weinh)	10.1002/advs.202503146	2025
	Complete Biodegradation of Diclofenac by New Bacterial Strains: Postulated Pathways and Degrading Enzymes.	Mohamed MSM, Asair AA, Fetyan NAH, Elnagdy SM.	Microorganisms	10.3390/microorganisms11061445	2023
	Physicochemical and Microbiological Water Quality Assessment of a Northwestern Algerian Dam: Detection of Ichtyopathogenic Bacteria.	Boumerdassi H, Djouadi LN, Hambli A, Fardeau ML, Ouzari HI, Nateche F.	Pol J Microbiol	10.33073/pjm-2023-020	2023
Metabolism	The genome of Alcaligenes aquatilis strain BU33N: Insights into hydrocarbon degradation capacity.	Mahjoubi M, Aliyu H, Cappello S, Naifer M, Souissi Y, Cowan DA, Cherif A.	PLoS One	10.1371/journal.pone.0221574	2019
	Alcaligenes aquatilis GTE53: Phosphate solubilising and bioremediation bacterium isolated from new biotope "phosphate sludge enriched-compost".	Haouas A, El Modafar C, Douira A, Ibnsouda-Koraichi S, Filali-Maltouf A, Moukhli A, Amir S.	Saudi J Biol Sci	10.1016/j.sjbs.2020.10.015	2021
Biotechnology	Comparative Genomics Reveals Novel Species and Insights into the Biotechnological Potential, Virulence, and Resistance of Alcaligenes.	Pedrosa-Silva F, Venancio TM.	Genes (Basel)	10.3390/genes14091783	2023
	A Preliminary Study on Microbiota Characteristics of Bronchoalveolar Lavage Fluid in Patients with Pulmonary Nodules Based on Metagenomic Next-Generation Sequencing.	Yuan Q, Wang X, Li Z, Guo W, Cheng H, Cao Q.	Biomedicines	10.3390/biomedicines11020631	2023
Genetics	Whole-genome sequencing of Alcaligenes sp. strain MMA: insight into the antibiotic and heavy metal resistant genes.	Sodhi KK, Singh CK, Kumar M, Singh DK.	Front Pharmacol	10.3389/fphar.2023.1144561	2023
Phylogeny	Culture dependent and independent characterization of endophytic bacteria in the seeds of highland barley.	Chen Y, Liang J, Zia A, Gao X, Wang Y, Zhang L, Xiang Q, Zhao K, Yu X, Chen Q, Penttinen P, Nyima T, Gu Y.	Front Microbiol	10.3389/fmicb.2022.981158	2022
	Degradation of azo dyes by Alcaligenes aquatilis 3c and its potential use in the wastewater treatment.	Ajaz M, Rehman A, Khan Z, Nisar MA, Hussain S.	AMB Express	10.1186/s13568-019-0788-3	2019
	Phenotypic and genotypic characterization of phosphate solubilizing bacteria and their efficiency on the growth of maize.	Pande A, Pandey P, Mehra S, Singh M, Kaushik S.	J Genet Eng Biotechnol	10.1016/j.jgeb.2017.06.005	2017
	Current overview of the mechanistic pathways and influence of physicochemical parameters on the microbial synthesis and applications of metallic nanoparticles.	Bhatnagar S, Aoyagi H.	Bioprocess Biosyst Eng	10.1007/s00449-025-03190-w	2025
	Metabolomic analysis of halotolerant endophytic bacterium Salinivibrio costicola isolated from Suaeda maritima (L.) dumort.	Lee J, Um S, Kim SH.	Front Mol Biosci	10.3389/fmolb.2022.967945	2022
Metabolism	Decolorization and discovery of metabolic pathway for the degradation of Mordant Black 11 dye by Klebsiella sp. MB398.	Tahir U, Yasmin A.	Braz J Microbiol	10.1007/s42770-021-00470-x	2021
Metabolism	Anoxic Biodegradation of Isosaccharinic Acids at Alkaline pH by Natural Microbial Communities.	Rout SP, Charles CJ, Doulgeris C, McCarthy AJ, Rooks DJ, Loughnane JP, Laws AP, Humphreys PN.	PLoS One	10.1371/journal.pone.0137682	2015
	Genetic Foundations of Direct Ammonia Oxidation (Dirammox) to N₂ and MocR-Like Transcriptional Regulator DnfR in Alcaligenes faecalis Strain JQ135.	Xu SQ, Qian XX, Jiang YH, Qin YL, Zhang FY, Zhang KY, Hong Q, He J, Miao LL, Liu ZP, Li DF, Liu SJ, Qiu JG.	Appl Environ Microbiol	10.1128/aem.02261-21	2022
	Single step biotransformation of corn oil phytosterols to boldenone by a newly isolated Pseudomonas aeruginosa.	Eisa M, El-Refai H, Amin M.	Biotechnol Rep (Amst)	10.1016/j.btre.2016.05.002	2016
Enzymology	UV Resistance of bacteria from the Kenyan Marine cyanobacterium Moorea producens.	Dzeha T, Nyiro C, Kardasopoulos D, Mburu D, Mwafaida J, Hall MJ, Burgess JG.	Microbiologyopen	10.1002/mbo3.697	2019
	The Development of the Bacterial Community of Brown Trout (Salmo trutta) during Ontogeny.	Keiz K, Ulrich S, Wenderlein J, Keferloher P, Wiesinger A, Neuhaus K, Lagkouvardos I, Wedekind H, Straubinger RK.	Microorganisms	10.3390/microorganisms11010211	2023
Genetics	Complete genome sequence and analysis of Alcaligenes faecalis strain Mc250, a new potential plant bioinoculant.	Felestrino EB, Sanchez AB, Caneschi WL, Lemes CGC, Assis RAB, Cordeiro IF, Fonseca NP, Villa MM, Vieira IT, Kamino LHY, do Carmo FF, da Silva AM, Thomas AM, Patane JSL, Ferreira FC, de Freitas LG, Varani AM, Ferro JA, Silva RS, Almeida NF, Garcia CCM, Setubal JC, Moreira LM.	PLoS One	10.1371/journal.pone.0241546	2020
Genetics	Genomic characterization of Kerstersia gyiorum SWMUKG01, an isolate from a patient with respiratory infection in China.	Li Y, Tang M, Wang G, Li C, Chen W, Luo Y, Zeng J, Hu X, Zhou Y, Gao Y, Zhang L.	PLoS One	10.1371/journal.pone.0214686	2019
Genetics	Genetic Determinants of Antagonistic Interactions and the Response of New Endophytic Strain Serratia quinivorans KP32 to Fungal Phytopathogens.	Chlebek D, Grebtsova V, Pinski A, Zur-Pinska J, Hupert-Kocurek K.	Int J Mol Sci	10.3390/ijms232415561	2022
	Mechanisms of halotolerant plant growth promoting Alcaligenes sp. involved in salt tolerance and enhancement of the growth of rice under salinity stress.	Fatima T, Mishra I, Verma R, Arora NK.	3 Biotech	10.1007/s13205-020-02348-5	2020
	Response surface methodology mediated optimization of Lignin peroxidase from Bacillus mycoides isolated from Simlipal Biosphere Reserve, Odisha, India.	Rath S, Paul M, Behera HK, Thatoi H.	J Genet Eng Biotechnol	10.1186/s43141-021-00284-2	2022
Enzymology	Decolorization and biodegradation of textile di-azo dye Acid Blue 113 by Pseudomonas stutzeri AK6.	Joshi AU, Hinsu AT, Kotadiya RJ, Rank JK, Andharia KN, Kothari RK.	3 Biotech	10.1007/s13205-020-02205-5	2020
	Diverse interactions between bacteria and microalgae: A review for enhancing harmful algal bloom mitigation and biomass processing efficiency.	Abate R, Oon YL, Oon YS, Bi Y, Mi W, Song G, Gao Y.	Heliyon	10.1016/j.heliyon.2024.e36503	2024
	Assessing Marine Microbial Induced Corrosion at Santa Catalina Island, California.	Ramirez GA, Hoffman CL, Lee MD, Lesniewski RA, Barco RA, Garber A, Toner BM, Wheat CG, Edwards KJ, Orcutt BN.	Front Microbiol	10.3389/fmicb.2016.01679	2016
Phylogeny	Alcaligenes endophyticus sp. nov., isolated from roots of Ammodendron bifolium.	Lu CY, Li YQ, Tian Y, Han MX, Rao MPN, Li YR, Zhu ZN, Wei DQ, An DD, Li WJ.	Int J Syst Evol Microbiol	10.1099/ijsem.0.001719	2017
Phylogeny	Castellaniella gen. nov., to accommodate the phylogenetic lineage of Alcaligenes defragrans, and proposal of Castellaniella defragrans gen. nov., comb. nov. and Castellaniella denitrificans sp. nov.	Kampfer P, Denger K, Cook AM, Lee ST, Jackel U, Denner EBM, Busse HJ.	Int J Syst Evol Microbiol	10.1099/ijs.0.63989-0	2006
Phylogeny	Alcaligenes aquatilis sp. nov., a novel bacterium from sediments of the Weser Estuary, Germany, and a salt marsh on Shem Creek in Charleston Harbor, USA.	Van Trappen S, Tan TL, Samyn E, Vandamme P	Int J Syst Evol Microbiol	10.1099/ijs.0.63849-0	2005

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#27871	IJSEM 2571 2005 ( DOI 10.1099/ijs.0.63849-0 , PubMed 16280529 )
#31582	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27871
#36307	; Curators of the CIP;
#59049	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 50924
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#116439	Collection of Institut Pasteur ; Curators of the CIP; CIP 108999
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Alcaligenes aquatilis (CCUG 50924, CIP 108999, LMG 22996)

Name and taxonomic classification

Morphology

Cell morphology

Pigmentation

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Metabolite utilization

Metabolite production

Enzymes

API zym

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_042649645

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

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