Pseudonocardia benzenivorans B5 is an aerobe, mesophilic, Gram-positive prokaryote that builds an aerial mycelium and was isolated from enrichment culture from soil.
Gram-positive rod-shaped aerobe mesophilic genome sequence 16S sequence| @ref 20215 |
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Last LPSN update
2025-12-16 09:50:05 |
| Domain Bacillati |
| Phylum Actinomycetota |
| Class Actinomycetes |
| Order Pseudonocardiales |
| Family Pseudonocardiaceae |
| Genus Pseudonocardia |
| Species Pseudonocardia benzenivorans |
| Full scientific name Pseudonocardia benzenivorans Kämpfer and Kroppenstedt 2004 |
| BacDive ID | Other strains from Pseudonocardia benzenivorans (1) | Type strain |
|---|---|---|
| 153296 | P. benzenivorans |
| @ref | Name | Growth | Medium link | Composition | |
|---|---|---|---|---|---|
| 11894 | GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) | Medium recipe at MediaDive  | Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water | ||
| 11894 | GYM+S MEDIUM (DSMZ Medium 214) | Medium recipe at MediaDive | Name: GYM+S MEDIUM (DSMZ Medium 214) Composition: Starch 20.0 g/l Agar 12.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water | ||
| 20097 | ISP 2 | Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes | |||
| 20097 | ISP 3 | Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms: | |||
| 20097 | ISP 4 | Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml | |||
| 20097 | ISP 5 | Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes | |||
| 20097 | ISP 7 | Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes | |||
| 20097 | ISP 6 | Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes |
| 30010 | Spore formationno |
| 67770 | Observationquinones: MK-8(H4) |
| @ref | Chebi-ID | Metabolite | Utilization activity | Kind of utilization tested | |
|---|---|---|---|---|---|
| 30010 | 17879 ChEBI | 4-hydroxybenzoate | + | carbon source | |
| 20097 | 22599 ChEBI | arabinose | - | ||
| 20097 | 62968 ChEBI | cellulose | + | ||
| 20097 | 28757 ChEBI | fructose | - | ||
| 20097 | 17234 ChEBI | glucose | + | ||
| 30010 | 17240 ChEBI | itaconate | + | carbon source | |
| 30010 | 24996 ChEBI | lactate | + | carbon source | |
| 20097 | 29864 ChEBI | mannitol | - | ||
| 30010 | 37684 ChEBI | mannose | + | carbon source | |
| 20097 | 17268 ChEBI | myo-inositol | + | ||
| 30010 | 28044 ChEBI | phenylalanine | + | carbon source | |
| 20097 | 16634 ChEBI | raffinose | + | ||
| 20097 | 26546 ChEBI | rhamnose | + | ||
| 30010 | 33942 ChEBI | ribose | + | carbon source | |
| 30010 | 17822 ChEBI | serine | + | carbon source | |
| 20097 | 17992 ChEBI | sucrose | - | ||
| 30010 | 27082 ChEBI | trehalose | + | carbon source | |
| 20097 | 18222 ChEBI | xylose | + | ||
| 30010 | 18222 ChEBI | xylose | + | carbon source |
| @ref | Value | Activity | Ec | |
|---|---|---|---|---|
| 68382 | acid phosphatase | - | 3.1.3.2 | from API zym |
| 68382 | alkaline phosphatase | - | 3.1.3.1 | from API zym |
| 68382 | alpha-chymotrypsin | - | 3.4.21.1 | from API zym |
| 68382 | alpha-fucosidase | - | 3.2.1.51 | from API zym |
| 68382 | alpha-galactosidase | - | 3.2.1.22 | from API zym |
| 68382 | alpha-glucosidase | + | 3.2.1.20 | from API zym |
| 68382 | alpha-mannosidase | - | 3.2.1.24 | from API zym |
| 68382 | beta-galactosidase | - | 3.2.1.23 | from API zym |
| 68382 | beta-glucosidase | - | 3.2.1.21 | from API zym |
| 68382 | beta-glucuronidase | - | 3.2.1.31 | from API zym |
| 68382 | cystine arylamidase | - | 3.4.11.3 | from API zym |
| 30010 | cytochrome oxidase | + | 1.9.3.1 | |
| 68382 | esterase (C 4) | + | from API zym | |
| 68382 | esterase lipase (C 8) | + | from API zym | |
| 68382 | leucine arylamidase | + | 3.4.11.1 | from API zym |
| 68382 | lipase (C 14) | - | from API zym | |
| 68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 | from API zym |
| 68382 | naphthol-AS-BI-phosphohydrolase | - | from API zym | |
| 68382 | trypsin | - | 3.4.21.4 | from API zym |
| 68382 | valine arylamidase | - | from API zym |
| Cat1 | Cat2 | Cat3 | |
|---|---|---|---|
| #Engineered | #Laboratory | #Lab enrichment | |
| #Environmental | #Terrestrial | #Soil |
Global distribution of 16S sequence AJ556156 (>99% sequence identity) for Pseudonocardia from Microbeatlas 
 Aquatic Samples |
241 |
 Soil Samples |
1233 |
 Animal Samples |
242 |
 Plant Samples |
132 |
| Total Samples | 1848 |
| @ref | Description | Assembly level | INSDC accession | NCBI tax ID | Score | |
|---|---|---|---|---|---|---|
| 124043 | ASM3954394v1 assembly for Pseudonocardia benzenivorans JCM 12694 | scaffold | GCA_039543945   | 228005 | 51.19 | |
| 124043 | ASM4266594v1 assembly for Pseudonocardia benzenivorans CCUG 49018 | contig | GCA_042665945 | 228005 | 11.65 | |
| 124043 | ASM4267918v1 assembly for Pseudonocardia benzenivorans CCUG 49018 | scaffold | GCA_042679185 | 228005 | |
| @ref | Description | Accession | Length | Database | NCBI tax ID | |
|---|---|---|---|---|---|---|
| 11894 | Pseudonocardia benzenivorans partial 16S rRNA gene, type strain B5 | AJ556156 | 1494 |  | 228005 |
| Topic | Title | Authors | Journal | DOI | Year | |
|---|---|---|---|---|---|---|
| Phylogeny | Pseudonocardia benzenivorans sp. nov. | Kampfer P, Kroppenstedt RM | Int J Syst Evol Microbiol | 10.1099/ijs.0.02825-0 | 2004 | |
How to citeIf you want to cite this particular strain cite the following doi:
https://doi.org/10.13145/bacdive13387.20251217.10
When using BacDive for research please cite the following paper
BacDive in 2025: the core database for prokaryotic strain data
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