Pseudonocardia alni subsp. alni DSM 44104 is a spore-forming bacterium that builds an aerial mycelium and was isolated from root nodules of Alnus incana.
spore-forming genome sequence 16S sequence Bacteria| @ref 20215 |
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Last LPSN update
2026-05-29 08:28:01 |
| Domain Bacteria |
| Phylum Actinomycetota |
| Class Actinomycetes |
| Order Pseudonocardiales |
| Family Pseudonocardiaceae |
| Genus Pseudonocardia |
| Species Pseudonocardia alni subsp. alni |
| Full scientific name Pseudonocardia alni subsp. alni (Evtushenko et al. 1989) Butdee et al. 2025 |
| @ref | Name | Growth | Medium link | Composition | |
|---|---|---|---|---|---|
| 11399 | BENNETT'S AGAR (DSMZ Medium 548) | Medium recipe at MediaDive  | Name: BENNETT'S AGAR (DSMZ Medium 548) Composition: Agar 15.0 g/l Glucose 10.0 g/l N-Z amine 2.0 g/l Yeast extract 1.0 g/l Beef extract 1.0 g/l Distilled water | ||
| 19627 | ISP 2 | Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes | |||
| 19627 | ISP 3 | Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms: | |||
| 19627 | ISP 4 | Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml | |||
| 19627 | ISP 5 | Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes | |||
| 19627 | ISP 6 | Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes | |||
| 19627 | ISP 7 | Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes |
| @ref | Spore description | Type of spore | Spore formation | |
|---|---|---|---|---|
| 19627 | Formation of spores, spore surface smooth, fragmentation | spore |
| @ref | Salt | Growth | Tested relation | Concentration | |
|---|---|---|---|---|---|
| 19627 | NaCl | positive | maximum | 2.5 % |
| 67770 | Observationquinones: MK-8(H4) |
| @ref | Chebi-ID | Metabolite | Utilization activity | Kind of utilization tested | |
|---|---|---|---|---|---|
| 68368 | 29016 ChEBI | arginine | + | hydrolysis | from API 20E |
| 68368 | 16947 ChEBI | citrate | + | assimilation | from API 20E |
| 68368 | 5291 ChEBI | gelatin | - | hydrolysis | from API 20E |
| 68368 | 25094 ChEBI | lysine | + | degradation | from API 20E |
| 68368 | 18257 ChEBI | ornithine | + | degradation | from API 20E |
| 68368 | 27897 ChEBI | tryptophan | - | energy source | from API 20E |
| 68368 | 16199 ChEBI | urea | + | hydrolysis | from API 20E |
| @ref | Value | Activity | Ec | |
|---|---|---|---|---|
| 68382 | acid phosphatase | + | 3.1.3.2 | from API zym |
| 68382 | alkaline phosphatase | + | 3.1.3.1 | from API zym |
| 68382 | alpha-chymotrypsin | - | 3.4.21.1 | from API zym |
| 68382 | alpha-fucosidase | - | 3.2.1.51 | from API zym |
| 68382 | alpha-galactosidase | - | 3.2.1.22 | from API zym |
| 68382 | alpha-glucosidase | + | 3.2.1.20 | from API zym |
| 68382 | alpha-mannosidase | - | 3.2.1.24 | from API zym |
| 68368 | arginine dihydrolase | + | 3.5.3.6 | from API 20E |
| 68382 | beta-galactosidase | + | 3.2.1.23 | from API zym |
| 68368 | beta-galactosidase | - | 3.2.1.23 | from API 20E |
| 68382 | beta-glucosidase | + | 3.2.1.21 | from API zym |
| 68382 | beta-glucuronidase | - | 3.2.1.31 | from API zym |
| 68382 | cystine arylamidase | - | 3.4.11.3 | from API zym |
| 68382 | esterase (C 4) | + | from API zym | |
| 68382 | esterase lipase (C 8) | + | from API zym | |
| 68368 | gelatinase | - | from API 20E | |
| 68382 | leucine arylamidase | + | 3.4.11.1 | from API zym |
| 68382 | lipase (C 14) | - | from API zym | |
| 68368 | lysine decarboxylase | + | 4.1.1.18 | from API 20E |
| 68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 | from API zym |
| 68382 | naphthol-AS-BI-phosphohydrolase | - | from API zym | |
| 68368 | ornithine decarboxylase | + | 4.1.1.17 | from API 20E |
| 68382 | trypsin | + | 3.4.21.4 | from API zym |
| 68368 | tryptophan deaminase | - | 4.1.99.1 | from API 20E |
| 68368 | urease | + | 3.5.1.5 | from API 20E |
| 68382 | valine arylamidase | + | from API zym |
Global distribution of 16S sequence Y08535 (>99% sequence identity) for Pseudonocardia from Microbeatlas 
 Aquatic Samples |
2199 |
 Soil Samples |
7999 |
 Animal Samples |
3094 |
 Plant Samples |
3610 |
| Total Samples | 16902 |
| @ref | Description | Assembly level | INSDC accession | BV-BRC accession | IMG accession | NCBI tax ID | Score | |
|---|---|---|---|---|---|---|---|---|
| 67770 | ASM281337v1 assembly for Pseudonocardia alni DSM 44104 | contig | GCA_002813375   | 33907.3  | 2627854291  | 33907 | 75.49 |
| @ref | Description | Accession | Length | Database | NCBI tax ID | |
|---|---|---|---|---|---|---|
| 20218 | Pseudonocardia alni gene for 16S ribosomal RNA, partial sequence, strain: DSM 44104 | AB915621 | 1193 | | 33907 | |
| 20218 | Pseudonocardia alni 16S ribosomal RNA gene, partial sequence | GU083568 | 1439 |  | 33907 | |
| 20218 | P.alni 16S rRNA gene | Y08535 | 1413 | | 33907 | |
| 20218 | Pseudonocardia alni 16S rRNA gene, strain IMSNU 20049T | AJ252823 | 1510 | | 33907 | |
| 20218 | A.alni (Ac901) 16S ribosomal RNA (partial) | X76954 | 1345 | | 33907 | |
| Topic | Title | Authors | Journal | DOI | Year | |
|---|---|---|---|---|---|---|
| Selenium nanoparticles conjugated with exopolysaccharides from marine hydrothermal vent crab-associated actinomycete Pseudonocardia alni SCSW01 and their antioxidant, antibacterial, and antibiofilm activities. | Munir Ahamed J, Dayalan S, Dahms HU, Khan MB, Upadhyaya U, Wu MT, Huang YL. | Biomater Adv | 10.1016/j.bioadv.2025.214593 | 2025 | | |
| Isolation of Pseudonocardia strains associated with the shallow water hydrothermal vent crab Xenograpsus testudinatus from a metal-rich environment: Biochemical characterization and enzymatic characterization, molecular identification, antibacterial, antibiofilm and antioxidant activity. | Munir Ahamed J, Dahms HU, Schizas NV, Rathinam AJ, Ouddane B, Huang YL. | Microb Pathog | 10.1016/j.micpath.2025.107457 | 2025 | | |
| Phylogeny | Identification of environmental Actinobacteria in buildings by means of chemotaxonomy, 16S rRNA sequencing, and MALDI-TOF MS. | Chudzik A, Jalkanen K, Taubel M, Szponar B, Pasciak M. | Microbiol Spectr | 10.1128/spectrum.03596-23 | 2024 | |
| Enabling Efficient Genetic Manipulations in a Rare Actinomycete Pseudonocardia alni Shahu. | Li J, Wang B, Yang Q, Si H, Zhao Y, Zheng Y, Peng W. | Front Microbiol | 10.3389/fmicb.2022.848964 | 2022 | | |
| Genetics | Targeted genome mining with GATOR-GC maps the evolutionary landscape of biosynthetic diversity. | Cediel-Becerra JDD, Cumsille A, Guerra S, Ding Y, de Crecy-Lagard V, Chevrette MG. | Nucleic Acids Res | 10.1093/nar/gkaf606 | 2025 | |
| Biotechnological and pharmaceutical potential of twenty-eight novel type strains of Actinomycetes from different environments worldwide. | Nouioui I, Boldt J, Zimmermann A, Makitrynskyy R, Potter G, Jando M, Doppner M, Kirstein S, Neumann-Schaal M, Gomez-Escribano JP, Nubel U, Mast Y. | Curr Res Microb Sci | 10.1016/j.crmicr.2024.100290 | 2024 | | |
| Genetics | Genome-Based Taxonomic Classification of the Phylum Actinobacteria. | Nouioui I, Carro L, Garcia-Lopez M, Meier-Kolthoff JP, Woyke T, Kyrpides NC, Pukall R, Klenk HP, Goodfellow M, Goker M. | Front Microbiol | 10.3389/fmicb.2018.02007 | 2018 | |
| Phylogeny | Pseudonocardia spirodelae sp. nov., isolated from duckweed and formal proposal to reclassify Pseudonocardia antarctica as a later heterotypic synonym of Pseudonocardia alni and reclassify Pseudonocardia carboxydivorans as Pseudonocardia alni subsp. carboxydivorans. | Butdee W, Saimee Y, Suriyachadkun C, Duangmal K. | Int J Syst Evol Microbiol | 10.1099/ijsem.0.006608 | 2025 | |
| Phylogeny | Pseudonocardia nematodicida sp. nov., isolated from mangrove sediment in Hainan, China. | Liu M, Xing SS, Yuan WD, Wei H, Sun QG, Lin XZ, Huang HQ, Bao SX. | Antonie Van Leeuwenhoek | 10.1007/s10482-015-0512-5 | 2015 | |
| Enzymology | Pseudonocardia parietis sp. nov., from the indoor environment. | Schafer J, Busse HJ, Kampfer P. | Int J Syst Evol Microbiol | 10.1099/ijs.0.009993-0 | 2009 | |
| Phylogeny | Pseudonocardia carboxydivorans sp. nov., a carbon monoxide-oxidizing actinomycete, and an emended description of the genus Pseudonocardia. | Park SW, Park ST, Lee JE, Kim YM. | Int J Syst Evol Microbiol | 10.1099/ijs.0.65765-0 | 2008 | |
| Enzymology | Pseudonocardia antarctica sp. nov. an Actinomycetes from McMurdo Dry Valleys, Antarctica. | Prabahar V, Dube S, Reddy GS, Shivaji S. | Syst Appl Microbiol | 10.1078/0723-2020-00249 | 2004 | |
| Phylogeny | Pseudonocardia tropica sp. nov., an endophytic actinomycete isolated from the stem of Maytenus austroyunnanensis. | Qin S, Zhu WY, Jiang JH, Klenk HP, Li J, Zhao GZ, Xu LH, Li WJ | Int J Syst Evol Microbiol | 10.1099/ijs.0.020099-0 | 2009 | |
How to citeIf you want to cite this particular strain cite the following doi:
https://doi.org/10.13145/bacdive13368.20260601.11
When using BacDive for research please cite the following paper
BacDive in 2025: the core database for prokaryotic strain data
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