Name: Winogradskyella arenosi R60
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 133478

Type strain:

Species: Winogradskyella arenosi

Strain Designation: R60

Culture col. no.: KMM 3968, NRIC 0748, JCM 15527, KCTC 62506, CIP 110076

Strain history: CIP <- 2009, KMM

NCBI tax ID(s): 533325 (species)

SI-ID 362863

JCM 15527, CIP 110076, CECT 7958

Section

Winogradskyella arenosi R60 is an aerobe, Gram-negative, motile bacterium that was isolated from Marine sediment sample collected from Peter the Great Bay.

Gram-negative
motile
rod-shaped
aerobe
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacteroidota*
	Class	*Flavobacteriia*
	Order	*Flavobacteriales*
	Family	*Flavobacteriaceae*
	Genus	*Winogradskyella*
	Species	**Winogradskyella arenosi**
	Full Scientific Name (LPSN)	Winogradskyella arenosi Romanenko et al. 2009

Information on morphological and physiological properties Morphology

[Ref.: #29124]	Gram stain	negative
[Ref.: #116486]	Gram stain	negative

[Ref.: #29124]	Cell length	2.1 µm

[Ref.: #29124]	Cell width	0.25 µm

[Ref.: #29124]	Cell shape	rod-shaped
[Ref.: #116486]	Cell shape	rod-shaped

[Ref.: #29124]	Motility	yes
[Ref.: #116486]	Motility	no

[Ref.: #29124]	Flagellum arrangement	gliding

[Ref.: #29124]

Pigment production

Information on culture and growth conditions Culture and growth conditions

[Ref.: #37691]

Culture medium

Marine agar (MA)

[Ref.: #37691]

Culture medium growth

[Ref.: #37691]

Culture medium composition

expand / minimize

[Ref.: #116486]

Culture medium

CIP Medium 13

[Ref.: #116486]

Culture medium growth

[Ref.: #116486]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #29124]	growth	04-35 °C
[Ref.: #29124]	optimum	26.5 °C
[Ref.: #37691]	growth	25 °C
[Ref.: #67770]	growth	25 °C

[Ref.: #29124]	Temperature range	mesophilic
[Ref.: #37691]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #29124]

Oxygen tolerance

aerobe

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #29124]		NaCl		growth	0.5-9.0	%
[Ref.: #29124]		NaCl		optimum	4.75	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #29124]	esculin ChEBI	+	hydrolysis
[Ref.: #29124]	glucose ChEBI	+	carbon source
[Ref.: #29124]	maltose ChEBI	+	carbon source
[Ref.: #29124]	mannitol ChEBI	+	carbon source
[Ref.: #29124]	mannose ChEBI	+	carbon source
[Ref.: #116486]	nitrate ChEBI	+	reduction
[Ref.: #116486]	nitrite ChEBI	-	reduction
[Ref.: #29124]	tween 20 ChEBI	+	carbon source
[Ref.: #29124]	tween 40 ChEBI	+	carbon source
[Ref.: #29124]	tween 80 ChEBI	+	carbon source

	Metabolite production	Metabolite	Production
[Ref.: #29124]		hydrogen sulfide ChEBI	yes
[Ref.: #116486]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #29124]	alkaline phosphatase	+	3.1.3.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #116486]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	+	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	+		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #116486]	oxidase	+
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #116486]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #116486]	12345	-	+	+	+	+	+	+	+	-	-	+	+	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #67770]	Sample type/isolated from	Marine sediment sample collected from Peter the Great Bay
[Ref.: #67770]	Geographic location (country and/or sea, region)	Sea of Japan

[Ref.: #116486]	Sample type/isolated from	Environment, Marine bottom sediments
[Ref.: #116486]	Country	Sea of Japan
[Ref.: #116486]	Isolation date	2002
* marker position based on {}

[Ref.: #69479]

Global distribution of 16S sequence AB438962 (>99% sequence identity) for Winogradskyella from Microbeatlas

Aquatic Samples	23
Soil Samples	33
Animal Samples	8
Plant Samples
Total Samples	64

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #116486]

Biosafety level

Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #29124]	Winogradskyella arenosi gene for 16S ribosomal RNA, partial sequence	AB438962	1430	GenBank	533325 tax ID

Availability in culture collections External links

[Ref.: #29124]

Culture collection no.

KMM 3968, NRIC 0748, JCM 15527, KCTC 62506, CIP 110076

[Ref.: #91460]

SI-ID 362863

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Winogradskyella sediminis sp. nov., isolated from marine sediment.	Zhang DC, Liu YX, Huang HJ, Weber K, Margesin R	Int J Syst Evol Microbiol	10.1099/ijsem.0.001157	2016	*
Phylogeny	Winogradskyella arenosi sp. nov., a member of the family Flavobacteriaceae isolated from marine sediments from the Sea of Japan.	Romanenko LA, Tanaka N, Frolova GM, Mikhailov VV	Int J Syst Evol Microbiol	10.1099/ijs.0.006809-0	2009	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#29124

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #25550 (see below)

#37691 ; Curators of the CIP;
#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#91460

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID362863.1 )

#116486 Collection of Institut Pasteur ; Curators of the CIP; CIP 110076

#25550

IJSEM 1443 2009 ( DOI 10.1099/ijs.0.006809-0 , PubMed 19502331 )

* These data were automatically processed and therefore are not curated

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