Name: Alcaligenes endophyticus AER 10
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 133384

Type strain:

Species: Alcaligenes endophyticus

Strain Designation: AER 10, AER10

Culture col. no.: DSM 100498, KCTC 42688

Strain history: <- W.-J. Li, YIM; AER10 <- X.-H. Shi

NCBI tax ID(s): 1929088 (species)

SI-ID 403250

DSM 100498

Section

Alcaligenes endophyticus AER 10 is a Gram-negative, motile, rod-shaped bacterium that forms circular colonies and was isolated from roots of Ammodendron bifolium obtained from Takeermohuer desert.

colony-forming
Gram-negative
motile
rod-shaped
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Betaproteobacteria*
	Order	*Burkholderiales*
	Family	*Alcaligenaceae*
	Genus	*Alcaligenes*
	Species	**Alcaligenes endophyticus**
	Full Scientific Name (LPSN)	Alcaligenes endophyticus Lu et al. 2017

Information on morphological and physiological properties Morphology

[Ref.: #25257]	Gram stain	negative

[Ref.: #25257]	Cell shape	rod-shaped

[Ref.: #25257]	Motility	yes

[Ref.: #25257]	Flagellum arrangement	peritrichous

[Ref.: #25257]	Colony color	pale yellow
[Ref.: #25257]	Colony shape	circular
[Ref.: #25257]	Cultivation medium used	LB medium

Information on culture and growth conditions Culture and growth conditions

[Ref.: #64147]

Culture medium

NUTRIENT AGAR (DSMZ Medium 1)

[Ref.: #64147]

Culture medium growth

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Culture medium link

Medium recipe provided by DSMZ

[Ref.: #64147]

Culture medium

TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)

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Culture medium growth

[Ref.: #64147]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #25257]

Culture medium

LB medium

[Ref.: #25257]

Culture medium growth

[Ref.: #64147]

Culture medium

CASO AGAR (MERCK 105458) (DSMZ Medium 220)

[Ref.: #64147]

Culture medium growth

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Culture medium link

Medium recipe at MediaDive

[Ref.: #64147]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #25257]	growth	10-45 °C
[Ref.: #64147]	growth	30 °C
[Ref.: #64147]	growth	28 °C

[Ref.: #64147]

Temperature range

mesophilic

	pH	Kind of pH		pH
[Ref.: #25257]			growth	5.0-9.0

Information on physiology and metabolism Physiology and metabolism

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #25257]		NaCl	maximum	10	%(w/v)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #25257]	(S)-lactate ChEBI	+	respiration
[Ref.: #25257]	2-oxobutanoate ChEBI	+/-	respiration
[Ref.: #25257]	2-oxoglutarate ChEBI	+	respiration
[Ref.: #25257]	3-hydroxybutyrate ChEBI	+	respiration
[Ref.: #25257]	3-hydroxybutyrate ChEBI	+/-	respiration
[Ref.: #25257]	4-hydroxyphenylacetic acid ChEBI	+/-	respiration
[Ref.: #25257]	acetate ChEBI	+/-	respiration
[Ref.: #25257]	acetoacetate ChEBI	+/-	respiration
[Ref.: #68369]	adipate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	arginine ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #25257]	aztreonam ChEBI	+	respiration
[Ref.: #25257]	bromosuccinate ChEBI	+/-	respiration
[Ref.: #25257]	citrate ChEBI	+	respiration
[Ref.: #25257]	D-fructose 6-phosphate ChEBI	-	respiration
[Ref.: #25257]	D-fucose ChEBI	-	respiration
[Ref.: #25257]	D-galacturonic acid ChEBI	+	respiration
[Ref.: #25257]	D-glucarate ChEBI	-	respiration
[Ref.: #25257]	D-gluconate ChEBI	-	respiration
[Ref.: #68369]	D-glucose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	fermentation	* from API 20NE
[Ref.: #25257]	D-glucuronate ChEBI	-	respiration
[Ref.: #68369]	D-mannitol ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	D-mannose ChEBI	-	assimilation	* from API 20NE
[Ref.: #25257]	D-serine ChEBI	-	respiration
[Ref.: #68369]	decanoate ChEBI	-	assimilation	* from API 20NE
[Ref.: #25257]	dextrin ChEBI	-	respiration
[Ref.: #68369]	esculin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #25257]	gamma-aminobutyric acid ChEBI	-	respiration
[Ref.: #25257]	gelatin ChEBI	-	hydrolysis
[Ref.: #68369]	gelatin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gluconate ChEBI	-	assimilation	* from API 20NE
[Ref.: #25257]	glucuronamide ChEBI	+/-	respiration
[Ref.: #25257]	L-alanine ChEBI	+/-	respiration
[Ref.: #68369]	L-arabinose ChEBI	-	assimilation	* from API 20NE
[Ref.: #25257]	L-aspartate ChEBI	-	respiration
[Ref.: #25257]	L-fucose ChEBI	+/-	respiration
[Ref.: #25257]	L-galactonate ChEBI	+	respiration
[Ref.: #25257]	L-glutamate ChEBI	+	respiration
[Ref.: #25257]	L-histidine ChEBI	-	respiration
[Ref.: #25257]	L-malate ChEBI	+	respiration
[Ref.: #25257]	L-pyroglutamic acid ChEBI	+	respiration
[Ref.: #25257]	lactone ChEBI	+	respiration
[Ref.: #25257]	lincomycin ChEBI	+	respiration
[Ref.: #25257]	malate ChEBI	+/-	respiration
[Ref.: #68369]	malate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	maltose ChEBI	-	assimilation	* from API 20NE
[Ref.: #25257]	methyl pyruvate ChEBI	+	respiration
[Ref.: #68369]	N-acetylglucosamine ChEBI	-	assimilation	* from API 20NE
[Ref.: #25257]	nitrate ChEBI	+	reduction
[Ref.: #68369]	nitrate ChEBI	-	reduction	* from API 20NE
[Ref.: #25257]	pectin ChEBI	-	respiration
[Ref.: #25257]	propionate ChEBI	+	respiration
[Ref.: #25257]	rifamycin sv ChEBI	+	respiration
[Ref.: #25257]	skimmed milk	+	degradation
[Ref.: #25257]	starch ChEBI	-	hydrolysis
[Ref.: #25257]	tetrazolium blue ChEBI	+	respiration
[Ref.: #25257]	tetrazolium violet ChEBI	+	respiration
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
[Ref.: #25257]	tween 20 ChEBI	-	hydrolysis
[Ref.: #25257]	tween 40 ChEBI	-	hydrolysis
[Ref.: #25257]	tween 60 ChEBI	-	hydrolysis
[Ref.: #25257]	tween 80 ChEBI	-	hydrolysis
[Ref.: #68369]	urea ChEBI	-	hydrolysis	* from API 20NE
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #25257]		hydrogen sulfide ChEBI	yes
[Ref.: #68369]		indole ChEBI	no	* from API 20NE

	Physiological tests	Metabolite	Indole test
[Ref.: #68369]		indole ChEBI	-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #25257]	acid phosphatase	+	3.1.3.2
[Ref.: #25257]	alkaline phosphatase	+	3.1.3.1
[Ref.: #25257]	alpha-chymotrypsin	+	3.4.21.1
[Ref.: #25257]	alpha-fucosidase	-	3.2.1.51
[Ref.: #25257]	alpha-galactosidase	-	3.2.1.22
[Ref.: #25257]	alpha-glucosidase	-	3.2.1.20
[Ref.: #25257]	alpha-mannosidase	-	3.2.1.24
[Ref.: #68369]	arginine dihydrolase	-	3.5.3.6	* from API 20NE
[Ref.: #25257]	beta-galactosidase	-	3.2.1.23
[Ref.: #25257]	beta-glucosidase	-	3.2.1.21
[Ref.: #68369]	beta-glucosidase	-	3.2.1.21	* from API 20NE
[Ref.: #25257]	beta-glucuronidase	-	3.2.1.31
[Ref.: #25257]	catalase	+	1.11.1.6
[Ref.: #25257]	cystine arylamidase	+	3.4.11.3
[Ref.: #25257]	cytochrome oxidase	+	1.9.3.1
[Ref.: #68369]	cytochrome oxidase	+	1.9.3.1	* from API 20NE
[Ref.: #25257]	esterase (C 4)	+
[Ref.: #25257]	esterase Lipase (C 8)	+
[Ref.: #68369]	gelatinase	-		* from API 20NE
[Ref.: #25257]	leucine arylamidase	+	3.4.11.1
[Ref.: #25257]	lipase (C 14)	+
[Ref.: #25257]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #25257]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #25257]	trypsin	+	3.4.21.4
[Ref.: #25257]	urease	-	3.5.1.5
[Ref.: #68369]	urease	-	3.5.1.5	* from API 20NE
[Ref.: #25257]	valine arylamidase	+
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #25257]

Fatty acid	Percentage
C18:1ω7c	25
C16:0	24.2
C16:1ω7c / C16:1ω6c	19.3
cyclo-C17:0	10.5

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #64147]	24220	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	+	-	not determinedn.d.
[Ref.: #64147]	25339	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	+	-	+

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #25257]	Sample type/isolated from	roots of Ammodendron bifolium obtained from Takeermohuer desert
[Ref.: #25257]	Geographic location (country and/or sea, region)	Takeermohuer desert (not found), Xinjiang Uygur Autonomous Region, northwest China
[Ref.: #25257]	Country	China
[Ref.: #25257]	Country ISO 3 Code	CHN
[Ref.: #25257]	Continent	Asia

[Ref.: #64147]	Sample type/isolated from	surface-sterilized roots of Ammodendron bifolium
[Ref.: #64147]	Geographic location (country and/or sea, region)	Xinjiang Uygur Autonomous Region, Takeermohuer desert
[Ref.: #64147]	Country	China
[Ref.: #64147]	Country ISO 3 Code	CHN
[Ref.: #64147]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental	#Terrestrial	#Desert
#Host	#Plants	#Shrub (Scrub)
#Host Body-Site	#Plant	#Root (Rhizome)

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence KR967368 (>99% sequence identity) for Alcaligenes endophyticus subclade from Microbeatlas

Aquatic Samples	341
Soil Samples	134
Animal Samples	1158
Plant Samples	84
Total Samples	1717

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #64147]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #64147]	Alcaligenes endophyticus strain AER10 16S ribosomal RNA gene, partial sequence	KR967368	1560	ENA	1929088 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Alcaligenes endophyticus DSM 100498	GCA_026344035	contig	GenBank	1929088 tax ID	*

[Ref.: #64147]

GC-content

53.3 mol%

high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Alcaligenes endophyticus DSM 100498 NCBI: GCA_026344035.1
[Ref.: #69480]	motile	yes	87.046	yes
[Ref.: #69480]	flagellated	yes	64.461	no
[Ref.: #69480]	gram-positive	no	98.144	no
[Ref.: #69480]	anaerobic	no	98.231	no
[Ref.: #69480]	aerobic	yes	80.75	no
[Ref.: #69480]	halophile	no	83.419	no
[Ref.: #69480]	spore-forming	no	95.873	no
[Ref.: #69480]	glucose-ferment	no	86.947	no
[Ref.: #69480]	thermophile	no	99.032	yes
[Ref.: #69480]	glucose-util	no	76.551	no

Availability in culture collections External links

[Ref.: #64147]

Culture collection no.

DSM 100498, KCTC 42688

[Ref.: #91424]

SI-ID 403250

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Alcaligenes endophyticus sp. nov., isolated from roots of Ammodendron bifolium.	Lu CY, Li YQ, Tian Y, Han MX, Rao MPN, Li YR, Zhu ZN, Wei DQ, An DD, Li WJ	Int J Syst Evol Microbiol	10.1099/ijsem.0.001719	2017	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#25257

C. Y. L. Lu, Y. Q.,Tian, Y.,Han, M. X.,Rao, M. P. N.,Li, Y. R.,Zhu, Z. N.,Wei, D. Q.,An, D. D.,Li, W. J.: Alcaligenes endophyticus sp. nov., isolated from roots of Ammodendron bifolium. IJSEM 67: 939 - 943 2017 ( DOI 10.1099/ijsem.0.001719 , PubMed 27959788 )

#64147

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 100498

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68369

Automatically annotated from API 20NE .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#91424

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID403250.1 )

* These data were automatically processed and therefore are not curated

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Alcaligenes endophyticus DSM 100498

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