Name: Paenibacillus qinlingensis TEGT-2
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 133371

Type strain:

Species: Paenibacillus qinlingensis

Strain Designation: TEGT-2

Culture col. no.: CCTCC AB 2015258, KCTC 33806

Strain history: <- Lei Zhang, Northwest A&F Univ., Yangling, Shannxi

NCBI tax ID(s): 1837343 (species)

Section

Paenibacillus qinlingensis TEGT-2 is an obligate aerobe, spore-forming, psychrophilic bacterium that forms circular colonies and was isolated from surface-sterilized roots of Sinopodophyllum hexandrum.

colony-forming
Gram-positive
motile
rod-shaped
spore-forming
obligate aerobe
psychrophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Paenibacillaceae*
	Genus	*Paenibacillus*
	Species	**Paenibacillus qinlingensis**
	Full Scientific Name (LPSN)	Paenibacillus qinlingensis Xin et al. 2017

Information on morphological and physiological properties Morphology

[Ref.: #25229]	Gram stain	positive
[Ref.: #67771]	Gram stain	positive

[Ref.: #25229]	Cell length	2.0-3.0 µm

[Ref.: #25229]	Cell width	0.8-1.0 µm

[Ref.: #25229]	Cell shape	rod-shaped
[Ref.: #67771]	Cell shape	rod-shaped

[Ref.: #25229]	Motility	yes
[Ref.: #67771]	Motility	yes

[Ref.: #25229]	Flagellum arrangement	peritrichous
[Ref.: #67771]	Flagellum arrangement	peritrichous

[Ref.: #25229]	Colony size	1.0-2.0 mm
[Ref.: #25229]	Colony color	cream-white
[Ref.: #25229]	Colony shape	circular
[Ref.: #25229]	Incubation period	2 days
[Ref.: #25229]	Cultivation medium used	R2A agar

Information on culture and growth conditions Culture and growth conditions

[Ref.: #25229]

Culture medium

nutrient agar (NA)

[Ref.: #25229]

Culture medium growth

[Ref.: #25229]

Culture medium

Reasoner's 2A agar (R2A)

[Ref.: #25229]

Culture medium growth

[Ref.: #25229]

Culture medium

MacConkey agar

[Ref.: #25229]

Culture medium growth

	Kind of temperature		Temperature
[Ref.: #25229]	no	growth	4 °C
[Ref.: #25229]	no	growth	37 °C
[Ref.: #67771]		growth	25 °C

[Ref.: #25229]	Temperature range	psychrophilic
[Ref.: #25229]	Temperature range	mesophilic
[Ref.: #67771]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #25229]	Oxygen tolerance	obligate aerobe
[Ref.: #67771]	Oxygen tolerance	aerobe

[Ref.: #25229]	Spore description	subterminal or paracentral position in swollen sporangia
[Ref.: #25229]	Type of spore	endospore
[Ref.: #25229]	Ability of spore formation	yes
[Ref.: #67771]	Type of spore	spore
[Ref.: #67771]	Ability of spore formation	yes

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #25229]		NaCl		growth	1	%

[Ref.: #67771]

Observation

quinones: MK-7

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #25229]	2-dehydro-D-gluconate ChEBI	-	builds acid from
[Ref.: #25229]	5-dehydro-D-gluconate ChEBI	-	builds acid from
[Ref.: #25229]	adipate ChEBI	-	assimilation
[Ref.: #25229]	amygdalin ChEBI	+	builds acid from
[Ref.: #25229]	arbutin ChEBI	+	builds acid from
[Ref.: #25229]	carboxymethylcellulose ChEBI	-	hydrolysis
[Ref.: #25229]	casein	-	hydrolysis
[Ref.: #25229]	cellobiose ChEBI	+	builds acid from
[Ref.: #25229]	citrate ChEBI	-	carbon source
[Ref.: #25229]	D-arabinose ChEBI	-	builds acid from
[Ref.: #25229]	D-arabitol ChEBI	-	builds acid from
[Ref.: #25229]	D-fructose ChEBI	+	builds acid from
[Ref.: #25229]	D-fucose ChEBI	-	builds acid from
[Ref.: #25229]	D-galactose ChEBI	+	builds acid from
[Ref.: #25229]	D-glucose ChEBI	-	builds gas from
[Ref.: #25229]	D-glucose ChEBI	+	assimilation
[Ref.: #25229]	D-glucose ChEBI	+	builds acid from
[Ref.: #25229]	D-lyxose ChEBI	-	builds acid from
[Ref.: #25229]	D-mannitol ChEBI	-	assimilation
[Ref.: #25229]	D-mannitol ChEBI	-	builds acid from
[Ref.: #25229]	D-mannose ChEBI	-	assimilation
[Ref.: #25229]	D-mannose ChEBI	+	builds acid from
[Ref.: #25229]	D-ribose ChEBI	-	builds acid from
[Ref.: #25229]	D-sorbitol ChEBI	-	builds acid from
[Ref.: #25229]	D-tagatose ChEBI	-	builds acid from
[Ref.: #25229]	D-trehalose ChEBI	+	builds acid from
[Ref.: #25229]	D-xylose ChEBI	+	builds acid from
[Ref.: #25229]	decanoate ChEBI	-	assimilation
[Ref.: #25229]	erythritol ChEBI	-	builds acid from
[Ref.: #25229]	esculin ChEBI	-	hydrolysis
[Ref.: #25229]	galactitol ChEBI	-	builds acid from
[Ref.: #25229]	gelatin ChEBI	-	hydrolysis
[Ref.: #25229]	gentiobiose ChEBI	+	builds acid from
[Ref.: #25229]	gluconate ChEBI	-	builds acid from
[Ref.: #25229]	glucose ChEBI	-	fermentation
[Ref.: #25229]	glycerol ChEBI	+	builds acid from
[Ref.: #25229]	glycogen ChEBI	+	builds acid from
[Ref.: #25229]	inulin ChEBI	-	builds acid from
[Ref.: #25229]	L-arabinose ChEBI	-	assimilation
[Ref.: #25229]	L-arabinose ChEBI	-	builds acid from
[Ref.: #25229]	L-arabitol ChEBI	-	builds acid from
[Ref.: #25229]	L-fucose ChEBI	+	builds acid from
[Ref.: #25229]	L-rhamnose ChEBI	-	builds acid from
[Ref.: #25229]	L-sorbose ChEBI	-	builds acid from
[Ref.: #25229]	L-xylose ChEBI	-	builds acid from
[Ref.: #25229]	lactose ChEBI	+	builds acid from
[Ref.: #25229]	malate ChEBI	-	assimilation
[Ref.: #25229]	maltose ChEBI	+	builds acid from
[Ref.: #25229]	maltose ChEBI	+	assimilation
[Ref.: #25229]	melezitose ChEBI	-	builds acid from
[Ref.: #25229]	melibiose ChEBI	+	builds acid from
[Ref.: #25229]	methyl alpha-D-glucopyranoside ChEBI	+	builds acid from
[Ref.: #25229]	methyl alpha-D-mannoside ChEBI	+	builds acid from
[Ref.: #25229]	methyl beta-D-xylopyranoside ChEBI	+	builds acid from
[Ref.: #25229]	myo-inositol ChEBI	-	builds acid from
[Ref.: #25229]	N-acetylglucosamine ChEBI	+	assimilation
[Ref.: #25229]	N-acetylglucosamine ChEBI	+	builds acid from
[Ref.: #25229]	nitrate ChEBI	+	reduction
[Ref.: #25229]	O-nitrophenyl-beta-D-galactopyranosid	+	hydrolysis
[Ref.: #25229]	phenylacetate ChEBI	-	assimilation
[Ref.: #25229]	potassium gluconate ChEBI	-	assimilation
[Ref.: #25229]	raffinose ChEBI	+	builds acid from
[Ref.: #25229]	ribitol ChEBI	-	builds acid from
[Ref.: #25229]	salicin ChEBI	+	builds acid from
[Ref.: #25229]	sodium citrate ChEBI	-	assimilation
[Ref.: #25229]	starch ChEBI	-	hydrolysis
[Ref.: #25229]	starch ChEBI	-	builds acid from
[Ref.: #25229]	sucrose ChEBI	+	builds acid from
[Ref.: #25229]	turanose ChEBI	+	builds acid from
[Ref.: #25229]	tyrosine ChEBI	-	hydrolysis
[Ref.: #25229]	xylitol ChEBI	+	builds acid from
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #25229]		acetoin ChEBI	no
[Ref.: #25229]		hydrogen sulfide ChEBI	no

	Metabolite	Voges-Proskauer-test	Methylred-test	Citrate test
[Ref.: #25229]	acetoin ChEBI	-
[Ref.: #25229]	citrate ChEBI			-
[Ref.: #25229]	glucose ChEBI		-

	Enzyme	Enzyme activity	EC number
[Ref.: #25229]	acid phosphatase	-	3.1.3.2
[Ref.: #25229]	alkaline phosphatase	+	3.1.3.1
[Ref.: #25229]	alpha-chymotrypsin	-	3.4.21.1
[Ref.: #25229]	alpha-fucosidase	-	3.2.1.51
[Ref.: #25229]	alpha-galactosidase	-	3.2.1.22
[Ref.: #25229]	alpha-glucosidase	+	3.2.1.20
[Ref.: #25229]	alpha-mannosidase	-	3.2.1.24
[Ref.: #25229]	arginine dihydrolase	-	3.5.3.6
[Ref.: #25229]	beta-galactosidase	+	3.2.1.23
[Ref.: #25229]	beta-glucosidase	+	3.2.1.21
[Ref.: #25229]	beta-glucuronidase	-	3.2.1.31
[Ref.: #25229]	catalase	+	1.11.1.6
[Ref.: #25229]	cystine arylamidase	-	3.4.11.3
[Ref.: #25229]	cytochrome oxidase	+	1.9.3.1
[Ref.: #25229]	esterase (C 4)	+
[Ref.: #25229]	esterase Lipase (C 8)	+
[Ref.: #25229]	leucine arylamidase	+	3.4.11.1
[Ref.: #25229]	lipase (C 14)	-
[Ref.: #25229]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #25229]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #25229]	trypsin	-	3.4.21.4
[Ref.: #25229]	urease	-	3.5.1.5
[Ref.: #25229]	valine arylamidase	-
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #25229]

Fatty acid	Percentage
C15:0 anteiso	62.5
C16:0 iso	9.4
C16:0	8.1
C17:0 anteiso	5.8

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #25229]	Sample type/isolated from	surface-sterilized roots of Sinopodophyllum hexandrum
[Ref.: #25229]	Geographic location (country and/or sea, region)	Qinling Mountains in shaanxi province
[Ref.: #25229]	Country	China
[Ref.: #25229]	Country ISO 3 Code	CHN
[Ref.: #25229]	Continent	Asia
[Ref.: #25229]	Geographic location	34.0000°/107.0000°
[Ref.: #25229]	Enrichment culture	0.1x trypticase soy agar (TSA; Difco)
[Ref.: #25229]	Enrichment culture composition	supplemented with 50 µg cycloheximide ml-1
[Ref.: #25229]	Enrichment culture duration	14 days
[Ref.: #25229]	Enrichment culture temperature	25 ̊C
[Ref.: #25229]	Isolation/enrichment procedure	carefully washed in running water to remove surface soil and subjected to a five-step surface sterilization procedure, cut into small fragments and macerated using a sterile pestle and mortar in sterile distilled water

[Ref.: #67771]	Sample type/isolated from	From the surface-sterilized root of `Sinopodophyllum hexandrum`
[Ref.: #67771]	Geographic location (country and/or sea, region)	Qinling Mountains, shaanxi province
[Ref.: #67771]	Country	China
[Ref.: #67771]	Country ISO 3 Code	CHN
[Ref.: #67771]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Host	#Plants	#Herbaceous plants (Grass,Crops)
#Host Body-Site	#Plant	#Root (Rhizome)
#Host Body-Site	#Plant	#Sterilized plant part

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence KX009025 (>99% sequence identity) for Paenibacillus from Microbeatlas

Aquatic Samples	9
Soil Samples	94
Animal Samples	8
Plant Samples	12
Total Samples	123

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #25229]	Paenibacillus sp. TEGT-2 16S ribosomal RNA gene, partial sequence	KX009025	1465	GenBank	1837343 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Paenibacillus qinlingensis TEGT-2	GCA_028746185	scaffold	GenBank	1837343 tax ID	*

[Ref.: #67771]

GC-content

46.6 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Paenibacillus qinlingensis TEGT-2 NCBI: GCA_028746185.1
[Ref.: #69480]	motile	yes	88.33	yes
[Ref.: #69480]	flagellated	yes	84.3	no
[Ref.: #69480]	gram-positive	yes	86.767	yes
[Ref.: #69480]	anaerobic	no	99.27	yes
[Ref.: #69480]	aerobic	yes	88.068	yes
[Ref.: #69480]	halophile	no	96.703	no
[Ref.: #69480]	spore-forming	yes	94.196	yes
[Ref.: #69480]	glucose-ferment	no	92.813	yes
[Ref.: #69480]	thermophile	no	99.79	no
[Ref.: #69480]	glucose-util	yes	89.559	yes

Availability in culture collections External links

[Ref.: #25229]

Culture collection no.

CCTCC AB 2015258, KCTC 33806

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Paenibacillus qinlingensis sp. nov., an indole-3-acetic acid-producing bacterium isolated from roots of Sinopodophyllum hexandrum (Royle) Ying.	Xin K, Li M, Chen C, Yang X, Li Q, Cheng J, Zhang L, Shen X	Int J Syst Evol Microbiol	10.1099/ijsem.0.001666	2017	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#25229

K. L. Xin, M.,Chen, C.,Yang, X.,Li, Q.,Cheng, J.,Zhang, L.,Shen, X.: Paenibacillus qinlingensis sp. nov., an indole-3-acetic acid-producing bacterium isolated from roots of Sinopodophyllum hexandrum (Royle) Ying. IJSEM 67: 589 - 595 2017 ( DOI 10.1099/ijsem.0.001666 , PubMed 27902261 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Paenibacillus qinlingensis in external resources:
SILVA
BRENDA
PANGAEA
StrainInfo
GBIF