Name: Williamsia spongiae B138
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 133111

Type strain:

Species: Williamsia spongiae

Strain Designation: B138

Culture col. no.: CBMAI 1094, DSM 46676

NCBI tax ID(s): 1087277 (species)

Section

Williamsia spongiae B138 is an aerobe, Gram-positive, rod-shaped bacterium that forms circular colonies and was isolated from Amphimedon viridis, a; marine sponge.

colony-forming
Gram-positive
rod-shaped
aerobe
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Mycobacteriales*
	Family	*Gordoniaceae*
	Genus	*Williamsia*
	Species	**Williamsia spongiae**
	Full Scientific Name (LPSN)	Williamsia spongiae Afonso de Menezes et al. 2017

Information on morphological and physiological properties Morphology

[Ref.: #25308]	Gram stain	positive

[Ref.: #25308]	Cell length	2.2-2.6 µm

[Ref.: #25308]	Cell width	0.6-0.8 µm

[Ref.: #25308]	Cell shape	rod-shaped

[Ref.: #25308]	Motility	no

[Ref.: #25308]	Colony color	orange
[Ref.: #25308]	Colony shape	circular
[Ref.: #25308]	Cultivation medium used	ISP 2 agar

Information on culture and growth conditions Culture and growth conditions

[Ref.: #25308]

Culture medium

ISP 2 agar

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Culture medium growth

	Temperatures	Kind of temperature		Temperature
[Ref.: #25308]			growth	10-45 °C
[Ref.: #25308]			optimum	30 °C

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Temperature range

mesophilic

	pH	Kind of pH		pH
[Ref.: #25308]			growth	4-10

Information on physiology and metabolism Physiology and metabolism

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Oxygen tolerance

aerobe

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Ability of spore formation

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #25308]		NaCl		growth	1-7	%(w/v)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #25308]	casein	+	hydrolysis
[Ref.: #25308]	cellobiose ChEBI	-	growth
[Ref.: #25308]	cellulose ChEBI	+	hydrolysis
[Ref.: #25308]	D-fructose ChEBI	+	growth
[Ref.: #25308]	D-galactose ChEBI	-	growth
[Ref.: #25308]	D-mannose ChEBI	-	growth
[Ref.: #25308]	D-ribose ChEBI	+	growth
[Ref.: #25308]	D-sorbitol ChEBI	-	growth
[Ref.: #25308]	erythritol ChEBI	-	growth
[Ref.: #25308]	gelatin ChEBI	-	hydrolysis
[Ref.: #25308]	glycerol ChEBI	-	growth
[Ref.: #25308]	guanine ChEBI	+	hydrolysis
[Ref.: #25308]	hypoxanthine ChEBI	+	hydrolysis
[Ref.: #25308]	L-arabinose ChEBI	-	growth
[Ref.: #25308]	lactate ChEBI	-	growth
[Ref.: #25308]	lactose ChEBI	-	growth
[Ref.: #25308]	malate ChEBI	+	growth
[Ref.: #25308]	mannitol ChEBI	-	growth
[Ref.: #25308]	myo-inositol ChEBI	-	growth
[Ref.: #25308]	oxalate ChEBI	-	growth
[Ref.: #25308]	raffinose ChEBI	-	growth
[Ref.: #25308]	rhamnose ChEBI	-	growth
[Ref.: #25308]	ribitol ChEBI	-	growth
[Ref.: #25308]	starch ChEBI	+	hydrolysis
[Ref.: #25308]	tween 20 ChEBI	+	hydrolysis
[Ref.: #25308]	tween 40 ChEBI	+	hydrolysis
[Ref.: #25308]	tween 60 ChEBI	+	hydrolysis
[Ref.: #25308]	tween 80 ChEBI	-	hydrolysis
[Ref.: #25308]	uric acid ChEBI	-	growth
[Ref.: #25308]	xanthine ChEBI	+	hydrolysis
[Ref.: #25308]	xylan ChEBI	+	hydrolysis
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic resistance	Concentration
[Ref.: #25308]	nalidixic acid ChEBI	yes	10	µg/mL
[Ref.: #25308]	novobiocin ChEBI	yes	10	µg/mL
[Ref.: #25308]	penicillin g ChEBI	yes	10	µg/mL

	Enzyme	Enzyme activity	EC number
[Ref.: #25308]	acid phosphatase	+	3.1.3.2
[Ref.: #25308]	alkaline phosphatase	+	3.1.3.1
[Ref.: #25308]	alpha-chymotrypsin	+	3.4.21.1
[Ref.: #25308]	alpha-fucosidase	-	3.2.1.51
[Ref.: #25308]	alpha-galactosidase	-	3.2.1.22
[Ref.: #25308]	alpha-glucosidase	+	3.2.1.20
[Ref.: #25308]	alpha-glucuronidase	-	3.2.1.139
[Ref.: #25308]	alpha-mannosidase	-	3.2.1.24
[Ref.: #25308]	beta-galactosidase	-	3.2.1.23
[Ref.: #25308]	beta-glucosidase	+	3.2.1.21
[Ref.: #25308]	catalase	+	1.11.1.6
[Ref.: #25308]	cystine arylamidase	+	3.4.11.3
[Ref.: #25308]	cytochrome oxidase	-	1.9.3.1
[Ref.: #25308]	esterase (C 4)	+
[Ref.: #25308]	esterase Lipase (C 8)	+
[Ref.: #25308]	leucine arylamidase	+	3.4.11.1
[Ref.: #25308]	lipase (C 14)	+
[Ref.: #25308]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #25308]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #25308]	trypsin	-	3.4.21.4
[Ref.: #25308]	valine arylamidase	-
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #25308]

Fatty acid	Percentage
10-methyl C18:0	24.8
C16:0	17.1
C18:1ω9c	14.3
C16:1ω7c / iso-C16:1ω7c 2-OH	11.9
C17:1ω8c	7
C18:0	6.8
C14:0	6
C17:0	5.9
10-Methyl C17:0	4.7
C15:1ω5c	1.5

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #25308]	Sample type/isolated from	Amphimedon viridis, a marine sponge
[Ref.: #25308]	Geographic location (country and/or sea, region)	beach areas Praia Guaeca, Sao Sebastiao region, Sao Paulo State
[Ref.: #25308]	Country	Brazil
[Ref.: #25308]	Country ISO 3 Code	BRA
[Ref.: #25308]	Continent	Middle and South America
[Ref.: #25308]	Geographic location	-25.0000°/-47.0000°
[Ref.: #25308]	Enrichment culture	tryptic soy agar (TSA, Oxoid)
[Ref.: #25308]	Enrichment culture composition	prepared with 80 % (v/v) artificial seawater
[Ref.: #25308]	Enrichment culture duration	4 weeks
[Ref.: #25308]	Enrichment culture temperature	25 ̊C
[Ref.: #25308]	Isolation/enrichment procedure	serial dilution method
* marker position based on {}

Isolation sources categories

#Host

#Invertebrates (Other)

#Porifera (Sponges)

What are isolation sources categories?

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #25308]	Williamsia spongiae strain B138 16S ribosomal RNA gene, partial sequence	JN615440	1477	GenBank	1087277 tax ID

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GC-content

73.3 mol%

Thermal denaturation, fluorometry

Availability in culture collections External links

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Culture collection no.

CBMAI 1094, DSM 46676

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Williamsia aurantiacus sp. nov. a novel actinobacterium producer of antimicrobial compounds isolated from the marine sponge.	de Menezes CBA, Afonso RS, de Souza WR, Parma MM, de Melo IS, Fugita FLS, Moraes LAB, Zucchi TD, Fantinatti-Garboggini F	Arch Microbiol	10.1007/s00203-019-01633-z	2019	*
Phylogeny	Williamsia spongiae sp. nov., an actinomycete isolated from the marine sponge Amphimedon viridis.	Afonso de Menezes CB, Afonso RS, Souza WR, Parma M, Melo IS, Zucchi TD, Fantinatti-Garboggini F	Int J Syst Evol Microbiol	10.1099/ijsem.0.001796	2017	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#25308

C. B. A. Afonso de Menezes, R. S.,Souza, W. R.,Parma, M.,Melo, I. S.,Zucchi, T. D.,Fantinatti-Garboggini, F.: Williamsia spongiae sp. nov., an actinomycete isolated from the marine sponge Amphimedon viridis. IJSEM 67: 1260 - 1265 2017 ( DOI 10.1099/ijsem.0.001796 , PubMed 28100308 )

* These data were automatically processed and therefore are not curated

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