Name: Lentzea waywayandensis DSM 44232
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 13308

Type strain:

Species: Lentzea waywayandensis

Culture col. no.: DSM 44232, AS 4.1646, ATCC 51594, IFO 14970, JCM 9114, NBRC 14970, NCIMB 13164, NRRL B-16159, CGMCC 4.1646, IMSNU 21344, KCTC 9400, VKM Ac-1970

Strain history: IFO 14970 <-- NRRL B-16159 <-- M. P. Lechevalier LLR-37z-15.

NCBI tax ID(s): 84724 (species)

SI-ID 43069

ATCC 51594, NRRL B-16159, DSM 44232, IFO 14970, JCM 9114, NCIMB 13164, VKM Ac-1970, AS 4.1646, KCTC 9400, NBRC 14970, CGMCC 4.1646

Section

Lentzea waywayandensis DSM 44232 is a spore-forming, mesophilic, Gram-positive bacterium that was isolated from soil.

Gram-positive
spore-forming
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Pseudonocardiales*
	Family	*Pseudonocardiaceae*
	Genus	*Lentzea*
	Species	**Lentzea waywayandensis**
	Full Scientific Name (LPSN)	Lentzea waywayandensis (Labeda and Lyons 1989) Labeda et al. 2001

Synonym

Saccharothrix waywayandensis

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #20026]	Colony color	Yellow orange (1037)
[Ref.: #20026]	Incubation period	10-14 days
[Ref.: #20026]	Cultivation medium used	ISP 2

[Ref.: #20026]	Colony color	Yellow orange (1037)
[Ref.: #20026]	Incubation period	10-14 days
[Ref.: #20026]	Cultivation medium used	ISP 3

[Ref.: #20026]	Colony color	Beige (1001)
[Ref.: #20026]	Incubation period	10-14 days
[Ref.: #20026]	Cultivation medium used	ISP 4

[Ref.: #20026]	Colony color	Yellow orange (1037)
[Ref.: #20026]	Incubation period	10-14 days
[Ref.: #20026]	Cultivation medium used	ISP 5

[Ref.: #20026]	Colony color	Beige (1001)
[Ref.: #20026]	Incubation period	10-14 days
[Ref.: #20026]	Cultivation medium used	ISP 6

[Ref.: #20026]	Colony color	Yellow orange (1037)
[Ref.: #20026]	Incubation period	10-14 days
[Ref.: #20026]	Cultivation medium used	ISP 7

[Ref.: #20026]	Multicellular complex forming ability	no
[Ref.: #20026]	Medium Name (multicellularity)	ISP 2

[Ref.: #20026]	Multicellular complex forming ability	no
[Ref.: #20026]	Medium Name (multicellularity)	ISP 3

[Ref.: #20026]	Multicellular complex forming ability	no
[Ref.: #20026]	Medium Name (multicellularity)	ISP 4

[Ref.: #20026]	Multicellular complex forming ability	no
[Ref.: #20026]	Medium Name (multicellularity)	ISP 5

[Ref.: #20026]	Multicellular complex forming ability	no
[Ref.: #20026]	Medium Name (multicellularity)	ISP 6

[Ref.: #20026]	Multicellular complex forming ability	no
[Ref.: #20026]	Medium Name (multicellularity)	ISP 7

Information on culture and growth conditions Culture and growth conditions

[Ref.: #11494]

Culture medium

GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

[Ref.: #11494]

Culture medium composition

expand / minimize

[Ref.: #20026]

Culture medium

ISP 2

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Culture medium growth

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Culture medium composition

expand / minimize

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Culture medium

ISP 3

[Ref.: #20026]

Culture medium growth

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Culture medium composition

expand / minimize

[Ref.: #20026]

Culture medium

ISP 5

[Ref.: #20026]

Culture medium growth

[Ref.: #20026]

Culture medium composition

expand / minimize

[Ref.: #20026]

Culture medium

ISP 7

[Ref.: #20026]

Culture medium growth

[Ref.: #20026]

Culture medium composition

expand / minimize

[Ref.: #20026]

Culture medium

ISP 6

[Ref.: #20026]

Culture medium growth

[Ref.: #20026]

Culture medium composition

expand / minimize

[Ref.: #20026]

Culture medium

ISP 4

[Ref.: #20026]

Culture medium growth

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Culture medium composition

expand / minimize

		Temperature
[Ref.: #11494]	growth	28 °C
[Ref.: #20026]	optimum	28 °C
[Ref.: #67770]	growth	28 °C

[Ref.: #11494]	Temperature range	mesophilic
[Ref.: #20026]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #69481]	Ability of spore formation	yes Confidence in %96
[Ref.: #69480]	Ability of spore formation	yes Confidence in %100

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #20026]		NaCl		maximum	2.5	%

[Ref.: #67770]

Observation

quinones: MK-9(H₄)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68368]	arginine ChEBI	+	hydrolysis	* from API 20E
[Ref.: #68369]	arginine ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68368]	citrate ChEBI	+	assimilation	* from API 20E
[Ref.: #68369]	D-glucose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	fermentation	* from API 20NE
[Ref.: #68369]	D-mannitol ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	D-mannose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	decanoate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	esculin ChEBI	+	hydrolysis	* from API 20NE
[Ref.: #68369]	gelatin ChEBI	+	hydrolysis	* from API 20NE, API 20E
[Ref.: #68369]	gluconate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	L-arabinose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68368]	lysine ChEBI	+	degradation	* from API 20E
[Ref.: #68369]	malate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	maltose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	N-acetylglucosamine ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	nitrate ChEBI	-	reduction	* from API 20NE
[Ref.: #68368]	ornithine ChEBI	+	degradation	* from API 20E
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE, API 20E
[Ref.: #68368]	urea ChEBI	+	hydrolysis	* from API 20E
[Ref.: #68369]	urea ChEBI	-	hydrolysis	* from API 20NE
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #68368]	acetoin ChEBI	yes	* from API 20E
[Ref.: #68368]	hydrogen sulfide ChEBI	no	* from API 20E
[Ref.: #68369]	indole ChEBI	no	* from API 20NE, API 20E

	Physiological tests	Metabolite	Voges-Proskauer-test	Indole test
[Ref.: #68368]		acetoin ChEBI	+		* from API 20E
[Ref.: #68369]		indole ChEBI		-	* from API 20NE, API 20E

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68368]	arginine dihydrolase	+	3.5.3.6	* from API 20E
[Ref.: #68369]	arginine dihydrolase	-	3.5.3.6	* from API 20NE
[Ref.: #68368]	beta-galactosidase	+	3.2.1.23	* from API 20E
[Ref.: #68369]	beta-glucosidase	+	3.2.1.21	* from API 20NE
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #68369]	gelatinase	+		* from API 20NE, API 20E
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68368]	lysine decarboxylase	+	4.1.1.18	* from API 20E
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API zym
[Ref.: #68368]	ornithine decarboxylase	+	4.1.1.17	* from API 20E
[Ref.: #68368]	tryptophan deaminase	-	4.1.99.1	* from API 20E
[Ref.: #68368]	urease	+	3.5.1.5	* from API 20E
[Ref.: #68369]	urease	-	3.5.1.5	* from API 20NE
	Only first 10 entries are displayed. Click here to see all.

API® 20E:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	beta-GalactosidaseONPG	Arginine dihydrolaseADH (Arg)	Lysine decarboxylaseLDC (Lys)	Ornithine decarboxylaseODC	Citrate utilizationCIT	H2S productionH2S	Urease/urea hydrolysisURE	Tryptophan deaminaseTDA (Trp)	Indole productionIND	Acetoin production (Voges Proskauer test)VP	Gelatinase/gelatin hydrolysisGEL	Fermentation/oxidation of D-glucoseGLU	Fermentation/oxidation of D-mannitolMAN	Fermentation/oxidation of inositolINO	Fermentation/oxidation of sorbitolSor	Fermentation/oxidation of L-rhamnoseRHA	Fermentation/oxidation of sucroseSAC	Fermentation/oxidation of melibioseMEL	Fermentation/oxidation of amygdalinAMY	Fermentation/oxidation of L-arabinoseARA	Cytochrome oxidaseOX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	Oxidative catabolisation of D-glucoseOF-O	Fermentation of D-glucoseOF-F
[Ref.: #20026]	611	+	+	+	+	+	-	+	-	-	+	+	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #11494]	24961	-	-	-	-	-	+	+	+	+	+	+	+	+	+	+	-	+/-	+	+/-	+/-	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #20026]	815	not determinedn.d.	+	-	+	-	-	-	-	+	-	+	+	-	-	-	-	+	+	-	-
[Ref.: #11494]	14227	-	+	+/-	-	-	+	+/-	+/-	+/-	+	-	-	-	+/-	-	+	-	+	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #11494]	Sample type/isolated from	soil
[Ref.: #11494]	Geographic location (country and/or sea, region)	New Jersey, Lake Waywayanda
[Ref.: #11494]	Country	USA
[Ref.: #11494]	Country ISO 3 Code	USA
[Ref.: #11494]	Continent	North America

[Ref.: #67770]	Sample type/isolated from	Soil
[Ref.: #67770]	Geographic location (country and/or sea, region)	Lake Waywayanda, NJ
[Ref.: #67770]	Country	USA
[Ref.: #67770]	Country ISO 3 Code	USA
[Ref.: #67770]	Continent	North America
* marker position based on {}

Isolation sources categories

#Environmental

#Terrestrial

#Soil

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #11494]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #20026]	Biosafety level	1	German classification

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Lentzea waywayandensis gene for 16S rRNA, strain: JCM 9114	AB020029	1476	ENA	84724 tax ID	*
[Ref.: #20218]	Lentzea waywayandensis strain NRRL B-16159T 16S ribosomal RNA gene, partial sequence	AF114813	1511	ENA	84724 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Lentzea waywayandensis DSM 44232	GCA_900115955	scaffold	GenBank	84724 tax ID
[Ref.: #66792]	Lentzea waywayandensis strain DSM 44232	84724.3	wgs	PATRIC	84724 tax ID	*
[Ref.: #66792]	Lentzea waywayandensis DSM 44232	2634166311	draft	JGI IMG/M	84724 tax ID	*

[Ref.: #11494]	GC-content	71.2 mol%	thermal denaturation, midpoint method (Tm)
[Ref.: #67770]	GC-content	68.9 mol%	genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Lentzea waywayandensis DSM 44232 NCBI: GCA_900115955.1
[Ref.: #69481]	spore-forming	yes	96	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Lentzea waywayandensis strain DSM 44232 PATRIC: 84724.3
[Ref.: #69480]	motile	no	91.633	no
[Ref.: #69480]	flagellated	no	96.998	no
[Ref.: #69480]	gram-positive	yes	87.336	no
[Ref.: #69480]	anaerobic	no	99.114	no
[Ref.: #69480]	halophile	no	86.16	no
[Ref.: #69480]	spore-forming	yes	93.257	no
[Ref.: #69480]	thermophile	no	98.76	no
[Ref.: #69480]	glucose-util	yes	89.231	yes
[Ref.: #69480]	aerobic	yes	90.821	no
[Ref.: #69480]	glucose-ferment	no	91.279	yes

Availability in culture collections External links

[Ref.: #11494]

Culture collection no.

DSM 44232, AS 4.1646, ATCC 51594, IFO 14970, JCM 9114, NBRC 14970, NCIMB 13164, NRRL B-16159, CGMCC 4.1646, IMSNU 21344, KCTC 9400, VKM Ac-1970

[Ref.: #82508]

SI-ID 43069

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Lentzea pudingi sp. nov., isolated from a weathered limestone sample in a karst area.	Cao C, Yuan B, Qin S, Jiang J, Tao F, Lian B	Int J Syst Evol Microbiol	10.1099/ijsem.0.002400	2017	*
Phylogeny	Lentzea cavernae sp. nov., an actinobacterium isolated from a karst cave sample, and emended description of the genus Lentzea.	Fang BZ, Han MX, Liu L, Zhang ZT, Liu WL, Shen JT, Wang Y, Zhang WQ, Wei DQ, Li WJ	Int J Syst Evol Microbiol	10.1099/ijsem.0.001958	2017	*
Phylogeny	Revival of the genus Lentzea and proposal for Lechevalieria gen. nov.	Labeda DP, Hatano K, Kroppenstedt RM, Tamura T	Int J Syst Evol Microbiol	10.1099/00207713-51-3-1045	2001	*

References

#11494

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 44232

#20026

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68368

Automatically annotated from API 20E .

#68369

Automatically annotated from API 20NE .

#68382

Automatically annotated from API zym .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#82508

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID43069.1 )

* These data were automatically processed and therefore are not curated

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Lentzea waywayandensis DSM 44232

Lentzea waywayandensis strain DSM 44232

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