Lentzea waywayandensis (DSM 44232, AS 4.1646, ATCC 51594)

Name: Lentzea waywayandensis (DSM 44232, AS 4.1646, ATCC 51594)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 13308

Type strain

Culture col. no. DSM 44232 AS 4.1646 ATCC 51594 IFO 14970 JCM 9114 7 more

NCBI tax ID(s) 84724

Special Collections DSMZ JCM KCTC Wink compendium

Links

Lentzea waywayandensis DSM 44232 is a mesophilic prokaryote that was isolated from soil.

mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 43069

ATCC 51594,NRRL B-16159,DSM 44232,IFO 14970,JCM 9114,NCIMB 13164,VKM Ac-1970,AS 4.1646,KCTC 9400,NBRC 14970,CGMCC 4.1646

@ref 20215

Last LPSN update 2025-12-16 09:50:03

Domain Bacillati

Phylum Actinomycetota

Class Actinomycetes

Order Pseudonocardiales

Family Pseudonocardiaceae

Genus Lentzea

Species Lentzea waywayandensis

Full scientific name Lentzea waywayandensis (Labeda and Lyons 1989) Labeda et al. 2001

Synonyms (1)

Saccharothrix waywayandensis

Morphology

Cell morphology

@ref	Motility	Confidence	Gram stain
125438		92
125439		98.8	positive

Colony morphology

@ref	Colony color	Incubation period	Medium used
20026	Yellow orange (1037)	10-14 days	ISP 2
20026	Yellow orange (1037)	10-14 days	ISP 3
20026	Beige (1001)	10-14 days	ISP 4
20026	Yellow orange (1037)	10-14 days	ISP 5
20026	Beige (1001)	10-14 days	ISP 6
20026	Yellow orange (1037)	10-14 days	ISP 7

Multicellular morphology

@ref	Forms multicellular complex	Medium name
20026		ISP 2
20026		ISP 3
20026		ISP 4
20026		ISP 5
20026		ISP 6
20026		ISP 7

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
11494	GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)	Medium recipe at MediaDive	Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
20026	ISP 2		Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
20026	ISP 3		Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
20026	ISP 5		Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
20026	ISP 7		Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
20026	ISP 6		Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
20026	ISP 4		Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
11494	positive	growth	28	mesophilic
20026	positive	optimum	28	mesophilic
67770	positive	growth	28	mesophilic

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
125439	obligate aerobe	97.1

Halophily

@ref	Salt	Growth	Tested relation	Concentration
20026	NaCl	positive	maximum	2.5 %

Observation

67770

Observationquinones: MK-9(H₄)

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68369	29016 ChEBI	arginine	-	hydrolysis	from API 20NE
68368	29016 ChEBI	arginine	+	hydrolysis	from API 20E
68368	16947 ChEBI	citrate	+	assimilation	from API 20E
68369	17634 ChEBI	D-glucose	+	assimilation	from API 20NE
68369	17634 ChEBI	D-glucose	-	fermentation	from API 20NE
68369	16899 ChEBI	D-mannitol	+	assimilation	from API 20NE
68369	16024 ChEBI	D-mannose	+	assimilation	from API 20NE
68369	27689 ChEBI	decanoate	-	assimilation	from API 20NE
68369	4853 ChEBI	esculin	+	hydrolysis	from API 20NE
68369	5291 ChEBI	gelatin	+	hydrolysis	from API 20NE
68368	5291 ChEBI	gelatin	+	hydrolysis	from API 20E
68369	24265 ChEBI	gluconate	+	assimilation	from API 20NE
68369	30849 ChEBI	L-arabinose	+	assimilation	from API 20NE
68368	25094 ChEBI	lysine	+	degradation	from API 20E
68369	25115 ChEBI	malate	+	assimilation	from API 20NE
68369	17306 ChEBI	maltose	+	assimilation	from API 20NE
68369	59640 ChEBI	N-acetylglucosamine	+	assimilation	from API 20NE
68369	17632 ChEBI	nitrate	-	reduction	from API 20NE
68368	18257 ChEBI	ornithine	+	degradation	from API 20E
68369	27897 ChEBI	tryptophan	-	energy source	from API 20NE
68368	27897 ChEBI	tryptophan	-	energy source	from API 20E
68369	16199 ChEBI	urea	-	hydrolysis	from API 20NE
68368	16199 ChEBI	urea	+	hydrolysis	from API 20E

Metabolite production

@ref	Chebi-ID	Metabolite
68368	15688 ChEBI	acetoin	from API 20E
68368	16136 ChEBI	hydrogen sulfide	from API 20E
68369	35581 ChEBI	indole	from API 20NE
68368	35581 ChEBI	indole	from API 20E

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Indole test
68368	15688 ChEBI	acetoin	+		from API 20E
68369	35581 ChEBI	indole		-	from API 20NE
68368	35581 ChEBI	indole		-	from API 20E

Enzymes

@ref	Value	Activity	Ec
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68369	arginine dihydrolase	-	3.5.3.6	from API 20NE
68368	arginine dihydrolase	+	3.5.3.6	from API 20E
68368	beta-galactosidase	+	3.2.1.23	from API 20E
68369	beta-glucosidase	+	3.2.1.21	from API 20NE
68382	beta-glucuronidase	-	3.2.1.31	from API zym
68369	gelatinase	+		from API 20NE
68368	gelatinase	+		from API 20E
68382	lipase (C 14)	-		from API zym
68368	lysine decarboxylase	+	4.1.1.18	from API 20E
68382	N-acetyl-beta-glucosaminidase	+	3.2.1.52	from API zym
68368	ornithine decarboxylase	+	4.1.1.17	from API 20E
68368	tryptophan deaminase	-	4.1.99.1	from API 20E
68369	urease	-	3.5.1.5	from API 20NE
68368	urease	+	3.5.1.5	from API 20E

API 20E

@ref	ONPG	ADH (Arg)	LDC (Lys)	ODC	CIT	H2S productionH2S	URE	TDA (Trp)	IND	Acetoin production (Voges Proskauer test)VP	GEL	GLU	MAN	INO	Sor	RHA	SAC	MEL	AMY	ARA	OX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	OF-O	OF-F
20026	+	+	+	+	+	-	+	-	-	+	+	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
20026	not determinedn.d.	+	-	+	-	-	-	-	+	-	+	+	-	-	-	-	+	+	-	-
11494	-	+	+/-	-	-	+	+/-	+/-	+/-	+	-	-	-	+/-	-	+	-	+	-	-

API 20NE

@ref	Reduction of nitratesNO3	TRP	GLU_ Ferm	ADH (Arg)	URE	ESC	GEL	PNPG	GLU_ Assim	ARA	MNE	MAN	NAG	MAL	GNT	CAP	ADI	MLT	CIT	PAC	OX
11494	-	-	-	-	-	+	+	+	+	+	+	+	+	+	+	-	+/-	+	+/-	+/-	not determinedn.d.

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Soil

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
11494	soil	New Jersey, Lake Waywayanda	USA	USA	North America
67770	Soil	Lake Waywayanda, NJ	USA	USA	North America

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
11494	1	Risk group (German classification) According to TRBA 466
20026	1	German classification According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	IMG-taxon 2634166311 annotated assembly for Lentzea waywayandensis DSM 44232	scaffold	GCA_900115955	84724.3	2634166311	84724	70.93

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
20218	Lentzea waywayandensis gene for 16S rRNA, strain: JCM 9114	AB020029	1476		84724
20218	Lentzea waywayandensis strain NRRL B-16159T 16S ribosomal RNA gene, partial sequence	AF114813	1511		84724

GC content

@ref	GC-content (mol%)	Method
11494	71.2	thermal denaturation, midpoint method (Tm)
67770	68.9	genome sequence analysis

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Lentzea waywayandensis DSM 44232
NCBI: GCA_900115955

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	yes	60.10	no
125439	motility	BacteriaNetⓘ	no	80.10	no
125439	gram_stain	BacteriaNetⓘ	positive	98.80	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	97.10	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Lentzea waywayandensis strain DSM 44232
PATRIC: 84724.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	87.74	no
125438	anaerobic	anaerobicⓘ	no	96.80	no
125438	aerobic	aerobicⓘ	yes	84.11	no
125438	spore-forming	spore-formingⓘ	yes	86.08	no
125438	thermophilic	thermophileⓘ	no	97.00	yes
125438	flagellated	motile2+ⓘ	no	92.00	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Characterization of a Novel Lentzea Species Isolated from the Kumtagh Desert and Genomic Insights into the Secondary Metabolite Potential of the Genus.	Wen Y, Li J, Qiao F, Luo W, Chen T, Liu G, Zhang W.	Microorganisms	10.3390/microorganisms13071628	2025
Enzymology	Nanocomposite Materials with Poly(l-lactic Acid) and Transition-Metal Dichalcogenide Nanosheets 2D-TMDCs WS₂.	Naffakh M, Fernandez M, Shuttleworth PS, Garcia AM, Moreno DA.	Polymers (Basel)	10.3390/polym12112699	2020
	The Degradative Capabilities of New Amycolatopsis Isolates on Polylactic Acid.	Decorosi F, Exana ML, Pini F, Adessi A, Messini A, Giovannetti L, Viti C.	Microorganisms	10.3390/microorganisms7120590	2019
Genetics	Biosynthetic novelty index reveals the metabolic potential of rare actinobacteria isolated from highly oligotrophic sediments.	Gonzalez-Salazar LA, Quezada M, Rodriguez-Orduna L, Ramos-Aboites H, Capon RJ, Souza-Saldivar V, Barona-Gomez F, Licona-Cassani C.	Microb Genom	10.1099/mgen.0.000921	2023
	Actinobacteria as Promising Candidate for Polylactic Acid Type Bioplastic Degradation.	Butbunchu N, Pathom-Aree W.	Front Microbiol	10.3389/fmicb.2019.02834	2019
	Preparation of poly(L-lactide) blends and biodegradation by Lentzea waywayandensis	Nair NR, Nampoothiri KM, Pandey A.	Biotechnol Lett		2012
Metabolism	Preparation of poly(L-lactide) blends and biodegradation by Lentzea waywayandensis.	Nair NR, Nampoothiri KM, Pandey A.	Biotechnol Lett	10.1007/s10529-012-1005-5	2012
	[Microecological mechanisms of red-leaf disease occurrence in Salvia miltiorrhiza Bge].	Duan JL, Shu ZM, Wei LZ, Fu LL, Xue QH.	Ying Yong Sheng Tai Xue Bao		2013
	The Effect of WS₂ Nanosheets on the Non-Isothermal Cold- and Melt-Crystallization Kinetics of Poly(l-lactic acid) Nanocomposites.	Naffakh M, Rica P, Moya-Lopez C, Castro-Osma JA, Alonso-Moreno C, Moreno DA.	Polymers (Basel)	10.3390/polym13132214	2021
	Assessing the Risk of Transfer of Microorganisms at the International Space Station Due to Cargo Delivery by Commercial Resupply Vehicles.	Mhatre S, Wood JM, Sielaff AC, Mora M, Duller S, Singh NK, Karouia F, Moissl-Eichinger C, Venkateswaran K.	Front Microbiol	10.3389/fmicb.2020.566412	2020
Genetics	Genome-Based Taxonomic Classification of the Phylum Actinobacteria.	Nouioui I, Carro L, Garcia-Lopez M, Meier-Kolthoff JP, Woyke T, Kyrpides NC, Pukall R, Klenk HP, Goodfellow M, Goker M.	Front Microbiol	10.3389/fmicb.2018.02007	2018
Phylogeny	Revival of the genus Lentzea and proposal for Lechevalieria gen. nov.	Labeda DP, Hatano K, Kroppenstedt RM, Tamura T	Int J Syst Evol Microbiol	10.1099/00207713-51-3-1045	2001
	Lentzea sokolovensis sp. nov., Lentzea kristufekii sp. nov. and Lentzea miocenica sp. nov., rare actinobacteria from Miocene lacustrine sediment of the Sokolov Coal Basin, Czech Republic.	Lara AC, Kotrbova L, Keller M, Nouioui I, Neumann-Schaal M, Mast Y, Chronakova A.	Int J Syst Evol Microbiol	10.1099/ijsem.0.006335	2024
Phylogeny	Lentzea pudingi sp. nov., isolated from a weathered limestone sample in a karst area.	Cao C, Yuan B, Qin S, Jiang J, Tao F, Lian B	Int J Syst Evol Microbiol	10.1099/ijsem.0.002400	2017
Phylogeny	Lentzea cavernae sp. nov., an actinobacterium isolated from a karst cave sample, and emended description of the genus Lentzea.	Fang BZ, Han MX, Liu L, Zhang ZT, Liu WL, Shen JT, Wang Y, Zhang WQ, Wei DQ, Li WJ	Int J Syst Evol Microbiol	10.1099/ijsem.0.001958	2017

References

#11494	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 44232
#20026	Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68368	Automatically annotated from API 20E .
#68369	Automatically annotated from API 20NE .
#68382	Automatically annotated from API zym .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Lentzea waywayandensis (DSM 44232, AS 4.1646, ATCC 51594)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Multicellular morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Halophily

Observation

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API 20E

API zym

API 20NE

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_900115955

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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