Name: Holdemania massiliensis AP2
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 132982

Type strain:

Species: Holdemania massiliensis

Strain Designation: AP2

Culture col. no.: DSM 26143, CSUR P195

Strain history: <- J.-C. Lagier, Timone Hospital, Marseille, France; AP2 <- A. Pfleiderer

NCBI tax ID(s): 1468449 (species)

SI-ID 399487

DSM 26143

Section

Holdemania massiliensis AP2 is a mesophilic bacterium that was isolated from human feces, 21-year-old female suffering from severe restrictive anorexia nervosa.

mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Erysipelotrichia*
	Order	*Erysipelotrichales*
	Family	*Erysipelotrichaceae*
	Genus	*Holdemania*
	Species	**Holdemania massiliensis**
	Full Scientific Name (LPSN)	Holdemania massiliensis Mishra et al. 2016

Synonym

"Candidatus Holdemania massiliensis"

Information on culture and growth conditions Culture and growth conditions

[Ref.: #24770]

Culture medium

PYG MEDIUM (MODIFIED) (DSMZ Medium 104)

[Ref.: #24770]

Culture medium growth

[Ref.: #24770]

Culture medium link

Medium recipe at MediaDive

[Ref.: #24770]

Culture medium composition

expand / minimize

[Ref.: #24770]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #24770]

Culture medium growth

[Ref.: #24770]

Culture medium link

Medium recipe at MediaDive

[Ref.: #24770]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #24770]			growth	37 °C

[Ref.: #24770]

Temperature range

mesophilic

Information on physiology and metabolism Physiology and metabolism

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68380]	arginine ChEBI	-	hydrolysis	* from API rID32A
[Ref.: #68380]	D-mannose ChEBI	-	fermentation	* from API rID32A
[Ref.: #68380]	L-glutamate ChEBI	-	degradation	* from API rID32A
[Ref.: #68380]	nitrate ChEBI	-	reduction	* from API rID32A
[Ref.: #68380]	raffinose ChEBI	-	fermentation	* from API rID32A
[Ref.: #68380]	tryptophan ChEBI	-	energy source	* from API rID32A
[Ref.: #68380]	urea ChEBI	-	hydrolysis	* from API rID32A

	Metabolite production	Metabolite	Production
[Ref.: #68380]		indole ChEBI	no	* from API rID32A

	Physiological tests	Metabolite	Indole test
[Ref.: #68380]		indole ChEBI	-	* from API rID32A

	Enzyme	Enzyme activity	EC number
[Ref.: #68380]	alanine arylamidase	-	3.4.11.2	* from API rID32A
[Ref.: #68380]	alkaline phosphatase	-	3.1.3.1	* from API rID32A
[Ref.: #68380]	alpha-arabinosidase	-	3.2.1.55	* from API rID32A
[Ref.: #68380]	alpha-fucosidase	+	3.2.1.51	* from API rID32A
[Ref.: #68380]	alpha-galactosidase	-	3.2.1.22	* from API rID32A
[Ref.: #68380]	alpha-glucosidase	+	3.2.1.20	* from API rID32A
[Ref.: #68380]	arginine dihydrolase	-	3.5.3.6	* from API rID32A
[Ref.: #68380]	beta-galactosidase	+	3.2.1.23	* from API rID32A
[Ref.: #68380]	beta-Galactosidase 6-phosphate	-		* from API rID32A
[Ref.: #68380]	beta-glucosidase	-	3.2.1.21	* from API rID32A
[Ref.: #68380]	beta-glucuronidase	-	3.2.1.31	* from API rID32A
[Ref.: #68380]	glutamate decarboxylase	-	4.1.1.15	* from API rID32A
[Ref.: #68380]	glutamyl-glutamate arylamidase	-		* from API rID32A
[Ref.: #68380]	glycin arylamidase	-		* from API rID32A
[Ref.: #68380]	histidine arylamidase	-		* from API rID32A
[Ref.: #68380]	L-arginine arylamidase	-		* from API rID32A
[Ref.: #68380]	leucine arylamidase	-	3.4.11.1	* from API rID32A
[Ref.: #68380]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API rID32A
[Ref.: #68380]	proline-arylamidase	-	3.4.11.5	* from API rID32A
[Ref.: #68380]	pyrrolidonyl arylamidase	+	3.4.19.3	* from API rID32A
[Ref.: #68380]	serine arylamidase	-		* from API rID32A
[Ref.: #68380]	tryptophan deaminase	-	4.1.99.1	* from API rID32A
[Ref.: #68380]	tyrosine arylamidase	+		* from API rID32A
[Ref.: #68380]	urease	-	3.5.1.5	* from API rID32A
	Only first 10 entries are displayed. Click here to see all.

API® rID32A:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Urease/urea hydrolysisURE	Arginine dihydrolaseADH (Arg)	alpha-Galactosidasealpha GAL	beta-Galactosidasebeta GAL	beta-Galactosidase 6-phosphatebeta GP	alpha-Glucosidasealpha GLU	beta-Glucosidasebeta GLU	alpha-Arabinosidasealpha ARA	beta-Glucuronidasebeta GUR	beta-N-Acetyl-beta-glucosaminidasebeta NAG	Fermentation of D-mannoseMNE	Fermentation of raffinoseRAF	Glutamate decarboxylaseGDC	alpha-Fucosidasealpha FUC	Reduction of nitrateNIT	Indole productionIND	Alkaline phosphatasePAL	L-arginine arylamidaseArgA	Proline arylamidaseProA	Leucyl glycin arylamidaseLGA	Phenylalanine arylamidasePheA	Leucine arylamidaseLeuA	Pyrrolidonyl arylamidasePyrA	Tyrosine arylamidaseTyrA	Alanine arylamidaseAlaA	Glycin arylamidaseGlyA	Histidine arylamidaseHisA	Glutamyl-glutamate arylamidaseGGA	Serine arylamidaseSerA
[Ref.: #24770]	13194	-	-	-	+	-	+	-	-	-	-	-	-	-	+	-	-	-	-	-	+	+	-	+	+	-	-	-	-	-
[Ref.: #24770]	12807	-	-	-	+	-	+	-	-	-	-	-	-	-	+	-	-	-	-	-	-	+/-	-	+	+	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #24770]	Sample type/isolated from	human feces, 21-year-old female suffering from severe restrictive anorexia nervosa
[Ref.: #24770]	Country	France
[Ref.: #24770]	Country ISO 3 Code	FRA
[Ref.: #24770]	Continent	Europe
[Ref.: #24770]	Geographic location	46.0000°/2.0000°
* marker position based on {}

Isolation sources categories

#Host	#Human	#Female
#Host Body Product	#Gastrointestinal tract	#Feces (Stool)

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence JX101683 (>99% sequence identity) for Holdemania massiliensis subclade from Microbeatlas

Aquatic Samples	185
Soil Samples	28
Animal Samples	12155
Plant Samples	3
Total Samples	12371

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #24770]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #24770]	Holdemania massiliensis AP2 16S ribosomal RNA gene, partial sequence	JX101683	1499	ENA	1211819 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Holdemania massiliensis AP2	GCA_000327285	scaffold	GenBank	1211819 tax ID	*
[Ref.: #66792]	Holdemania massiliensis AP2	2551306369	draft	JGI IMG/M	1211819 tax ID	*

[Ref.: #24770]

GC-content

47.1 mol%

sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Holdemania massiliensis AP2 AP2 NCBI: GCA_000327285.1
[Ref.: #69480]	motile	no	92.555	no
[Ref.: #69480]	flagellated	no	97.467	no
[Ref.: #69480]	gram-positive	yes	95.295	no
[Ref.: #69480]	anaerobic	yes	97.957	no
[Ref.: #69480]	aerobic	no	97.986	no
[Ref.: #69480]	halophile	no	77.527	no
[Ref.: #69480]	spore-forming	no	77.987	no
[Ref.: #69480]	thermophile	no	97.99	yes
[Ref.: #69480]	glucose-util	yes	91.228	no
[Ref.: #69480]	glucose-ferment	yes	64.207	no

Availability in culture collections External links

[Ref.: #24770]

Culture collection no.

DSM 26143, CSUR P195

[Ref.: #91258]

SI-ID 399487

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#24770

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 26143

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68380

Automatically annotated from API rID32A .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#91258

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID399487.1 )

* These data were automatically processed and therefore are not curated

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Holdemania massiliensis AP2 AP2

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