Name: Methanoculleus hydrogenitrophicus HC
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 132893

Type strain:

Species: Methanoculleus hydrogenitrophicus

Strain Designation: HC

Culture col. no.: DSM 25996, CGMCC 1.5146, JCM 16311

Strain history: X. Dong strain HC.

NCBI tax ID(s): 648796 (species)

SI-ID 401531

DSM 25996

Section

Methanoculleus hydrogenitrophicus HC is an anaerobe, mesophilic, Gram-negative archaeon that was isolated from soil.

coccus-shaped
Gram-negative
anaerobe
mesophilic
16S sequence
Archaea
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Archaea"
	Phylum	*Methanobacteriota*
	Class	"Methanomicrobia"
	Order	*Methanomicrobiales*
	Family	*Methanomicrobiaceae*
	Genus	*Methanoculleus*
	Species	**Methanoculleus hydrogenitrophicus**
	Full Scientific Name (LPSN)	Methanoculleus hydrogenitrophicus Tian et al. 2010

Information on morphological and physiological properties Morphology

[Ref.: #29709]	Gram stain	negative

[Ref.: #29709]	Cell length	0.8-2 µm

[Ref.: #29709]	Cell width	0.8-2 µm

[Ref.: #29709]	Cell shape	coccus-shaped

[Ref.: #29709]	Motility	no

[Ref.: #29709]

Pigment production

Information on culture and growth conditions Culture and growth conditions

[Ref.: #24681]

Culture medium

METHANOCULLEUS SP. MEDIUM (DSMZ Medium 141b)

[Ref.: #24681]

Culture medium growth

[Ref.: #24681]

Culture medium link

Medium recipe at MediaDive

[Ref.: #24681]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #24681]	growth	37 °C
[Ref.: #29709]	growth	18-45 °C
[Ref.: #29709]	optimum	37 °C
[Ref.: #67770]	growth	37 °C

[Ref.: #24681]	Temperature range	mesophilic
[Ref.: #29709]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #29709]			optimum	6.6
[Ref.: #29709]			growth	5-8.5

Information on physiology and metabolism Physiology and metabolism

[Ref.: #29709]

Oxygen tolerance

anaerobe

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #29709]		NaCl		growth	0.29-3	%
[Ref.: #29709]		NaCl		optimum	1.16	%

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #24681]	Sample type/isolated from	soil
[Ref.: #24681]	Geographic location (country and/or sea, region)	Tibetan plateau, Zoige wetland
[Ref.: #24681]	Country	China
[Ref.: #24681]	Country ISO 3 Code	CHN
[Ref.: #24681]	Continent	Asia
[Ref.: #24681]	Geographic location	33.9333°/102.8670°

[Ref.: #67770]	Sample type/isolated from	Soil of the Zoige wetland on the Tibetan plateau
[Ref.: #67770]	Country	China
[Ref.: #67770]	Country ISO 3 Code	CHN
[Ref.: #67770]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental

#Terrestrial

#Soil

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence FJ977567 (>99% sequence identity) for Methanoculleus hydrogenitrophicus subclade from Microbeatlas

Aquatic Samples	1
Soil Samples
Animal Samples
Plant Samples
Total Samples	1

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #24681]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #24681]	Methanoculleus hydrogenitrophicus strain HC 16S ribosomal RNA gene, partial sequence	FJ977567	1434	ENA	648796 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Methanoculleus hydrogenitrophicus DSM 25996	2913463320	draft	JGI IMG/M	648796 tax ID	*

[Ref.: #24681]	GC-content	60.2 mol%	thermal denaturation, midpoint method (Tm)
[Ref.: #29709]	GC-content	60.2 mol%

Availability in culture collections External links

[Ref.: #24681]

Culture collection no.

DSM 25996, CGMCC 1.5146, JCM 16311

[Ref.: #91173]

SI-ID 401531

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Methanoculleus hydrogenitrophicus sp. nov., a methanogenic archaeon isolated from wetland soil.	Tian J, Wang Y, Dong X	Int J Syst Evol Microbiol	10.1099/ijs.0.019273-0	2009	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#24681

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 25996

#29709

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26092 (see below)

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#91173

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID401531.1 )

#26092

IJSEM 2165 2010 ( DOI 10.1099/ijs.0.019273-0 , PubMed 19897615 )

* These data were automatically processed and therefore are not curated

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