Ruoffia tabacinasalis (DSM 104272, CCUG 30090, CIP 106117)

Name: Ruoffia tabacinasalis (DSM 104272, CCUG 30090, CIP 106117)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 132844

Type strain

Strain Designation GF112B

Culture col. no. DSM 104272 CCUG 30090 CIP 106117 ATCC 700838 CCM 4934 1 more

NCBI tax ID(s) 87458

Special Collections DSMZ CCUG CIP

Links

Ruoffia tabacinasalis GF112B is a facultative anaerobe, mesophilic, Gram-positive prokaryote that was isolated from Industrial tobacco, snuff, contaminant.

Gram-positive coccus-shaped facultative anaerobe mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 13444

LMG 19533,ATCC 700838,CCUG 30090,CIP 106117,CCM 4934,NCIMB 13890,DSM 104272

@ref 20215

Last LPSN update 2025-12-16 10:01:37

Domain Bacillati

Phylum Bacillota

Class Bacilli

Order Lactobacillales

Family Aerococcaceae

Genus Ruoffia

Species Ruoffia tabacinasalis

Full scientific name Ruoffia tabacinasalis (Collins et al. 1999) Fotedar et al. 2021

Synonyms (1)

Facklamia tabacinasalis

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility
123406	positive	coccus-shaped

Colony morphology

123406

Hemolysis ability1

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
24632	COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)	Medium recipe at MediaDive	Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
39476	MEDIUM 6 - Columbia agar with 10 % horse blood		Distilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)
123406	CIP Medium 6	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
24632	positive	growth	30	mesophilic
39476	positive	growth	37	mesophilic
123406	positive	growth	25-37	mesophilic
123406	negative	growth	5
123406	negative	growth	41
123406	negative	growth	45

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance
123406	facultative anaerobe
125439	obligate aerobe

Halophily

@ref	Salt	Growth	Tested relation	Concentration
123406	NaCl	positive	growth	6.5 %

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
123406	4853 ChEBI	esculin	-	hydrolysis
123406	606565 ChEBI	hippurate	-	hydrolysis
123406	17632 ChEBI	nitrate	-	reduction
123406	16301 ChEBI	nitrite	-	reduction

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test
123406	15688 ChEBI	acetoin	-
123406	17234 ChEBI	glucose

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	-	3.1.3.2	from API zym
123406	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	-	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	-	3.2.1.23	from API zym
123406	beta-galactosidase	-	3.2.1.23
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
123406	catalase	-	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
68382	esterase (C 4)	-		from API zym
68382	esterase lipase (C 8)	+		from API zym
123406	gamma-glutamyltransferase	-	2.3.2.2
68382	leucine arylamidase	-	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
123406	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
123406	ornithine decarboxylase	-	4.1.1.17
123406	oxidase	-
68382	trypsin	-	3.4.21.4	from API zym
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
123406	-	-	-	+	-	-	-	-	-	-	-	+	-	-	-	+	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2
#Engineered	#Contamination
#Engineered	#Industrial

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
24632	Industrial tobacco, snuff, contaminant	Stockholm	Sweden	SWE	Europe
49894	Industrial tobacco,snuff,contaminant		Sweden	SWE	Europe
123406	Environment, Industrial tobacco, snuff, contaminant	Stockholm	Sweden	SWE	Europe

Taxonmaps

69479

Global distribution of 16S sequence Y17820 (>99% sequence identity) for Facklamia tabacinasalis subclade from Microbeatlas

Aquatic Samples	3694
Soil Samples	4365
Animal Samples	33973
Plant Samples	1110
Total Samples	43142

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
24632	1	Risk group (German classification)
123406	1	Risk group (French classification)

Sequence information

Genome sequences

@ref	Description	Assembly level	IMG accession	NCBI tax ID	Score
66792	Facklamia tabacinasalis DSM 104272	complete	2913588957	87458	82.35
66792	Facklamia tabacinasalis strain DSM 104272	contig		87458	60.03
66792	ASM1586328v1 assembly for Ruoffia tabacinasalis DSM 104272	contig		87458	57.64
124043	ASM4265298v1 assembly for Ruoffia tabacinasalis CCUG 30090	scaffold		87458	56.85

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
24632	Facklamia sp. CCUG30090 16S rRNA gene	Y17820	1502		87458

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Ruoffia tabacinasalis DSM 104272
NCBI: GCA_015863285

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	yes	76.50	no
125439	motility	BacteriaNetⓘ	yes	65.80	no
125439	gram_stain	BacteriaNetⓘ	variable	72.00	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	97.70	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Facklamia tabacinasalis strain DSM 104272
PATRIC: 87458.4

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	85.43	no
125438	anaerobic	anaerobicⓘ	no	90.01	yes
125438	aerobic	aerobicⓘ	no	88.63	yes
125438	spore-forming	spore-formingⓘ	no	75.42	no
125438	thermophilic	thermophileⓘ	no	97.50	yes
125438	flagellated	motile2+ⓘ	no	84.00	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Isolation, Physiological Characterization, and Antibiotic Susceptibility Testing of Fast-Growing Bacteria from the Sea-Affected Temporary Meltwater Ponds in the Thala Hills Oasis (Enderby Land, East Antarctica).	Akulava V, Miamin U, Akhremchuk K, Valentovich L, Dolgikh A, Shapaval V.	Biology (Basel)	10.3390/biology11081143	2022
Phylogeny	A 16S rRNA Gene and Draft Genome Database for the Murine Oral Bacterial Community.	Joseph S, Aduse-Opoku J, Hashim A, Hanski E, Streich R, Knowles SCL, Pedersen AB, Wade WG, Curtis MA.	mSystems	10.1128/msystems.01222-20	2021
Phylogeny	Reclassification of Facklamia ignava, Facklamia sourekii and Facklamia tabacinasalis as Falseniella ignava gen. nov., comb. nov., Hutsoniella sourekii gen. nov., comb. nov., and Ruoffia tabacinasalis gen. nov., comb. nov., and description of Ruoffia halotolerans sp. nov., isolated from hypersaline Inland Sea of Qatar.	Fotedar R, Sankaranarayanan K, Caldwell ME, Zeyara A, Al Malki A, Kaul R, Al Shamari H, Ali M, Al Marri M, Lawson PA	Antonie Van Leeuwenhoek	10.1007/s10482-021-01587-7	2021
Phylogeny	Facklamia tabacinasalis sp. nov., from powdered tobacco.	Collins MD, Hutson RA, Falsen E, Sjoden B	Int J Syst Bacteriol	10.1099/00207713-49-3-1247	1999

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#24632	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 104272
#39476	; Curators of the CIP;
#49894	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 30090
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#123406	Collection of Institut Pasteur ; Curators of the CIP; CIP 106117
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Ruoffia tabacinasalis (DSM 104272, CCUG 30090, CIP 106117)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Halophily

Metabolite utilization

Metabolite tests

Enzymes

API zym

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

16S sequences

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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