Name: Tepidibacillus decaturensis Z9
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 132643

Type strain:

Species: Tepidibacillus decaturensis

Strain Designation: Z9

Culture col. no.: DSM 103037, ATCC BAA 2644, JCM 31418

Strain history: B. W. Fouke; Univ. of Illinois Urbana-Champaign, USA; Z9.

NCBI tax ID(s): 1413211 (species)

Section

Tepidibacillus decaturensis Z9 is a microaerophile, spore-forming, Gram-negative bacterium that forms circular colonies and was isolated from deep subsurface groundwater.

colony-forming
Gram-negative
motile
rod-shaped
spore-forming
microaerophile
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	07-12-2022 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Tepidibacillus*
	Species	**Tepidibacillus decaturensis**
	Full Scientific Name (PNU)	Tepidibacillus decaturensis Dong et al. 2016

Information on morphological and physiological properties Morphology

[Ref.: #24926]	Gram stain	negative
[Ref.: #24926]	Cell length	1.0-10.0 µm
[Ref.: #24926]	Cell width	0.3 µm
[Ref.: #24926]	Cell shape	rod-shaped
[Ref.: #24926]	Motility	yes
[Ref.: #24926]	Flagellum arrangement	monotrichous, polar

[Ref.: #24926]	Colony size	0.5-1.0 mm
[Ref.: #24926]	Colony color	beige
[Ref.: #24926]	Colony shape	circular
[Ref.: #24926]	Incubation period	7 days

Information on culture and growth conditions Culture and growth conditions

[Ref.: #24432]

Culture medium

DEFERRISOMA MEDIUM (DSMZ Medium 1451)

[Ref.: #24432]

Culture medium growth

[Ref.: #24432]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #24432]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #24432]	growth	37 °C
[Ref.: #24926]	growth	20.0-60.0 °C
[Ref.: #24926]	optimum	30.0-40.0 °C
[Ref.: #67770]	growth	37 °C

[Ref.: #24432]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH	pH range
[Ref.: #24926]			optimum	5.2-5.8
[Ref.: #24926]			growth	5.0-8.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #24926]

Oxygen tolerance

microaerophile

[Ref.: #24926]	Type of spore	endospore
[Ref.: #24926]	Ability of spore formation	yes

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #24926]		NaCl		growth	1.0-5.0	%(w/v)
[Ref.: #24926]		NaCl		optimum	2.5	%(w/v)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #24926]	betaine ChEBI	-	fermentation
[Ref.: #24926]	elemental sulfur ChEBI	-	electron acceptor
[Ref.: #24926]	ferric citrate ChEBI	+	electron acceptor
[Ref.: #24926]	ferrihydrite	+	electron acceptor
[Ref.: #24926]	fructose ChEBI	+	fermentation
[Ref.: #24926]	galactose ChEBI	+	fermentation
[Ref.: #24926]	glucosamine ChEBI	+	fermentation
[Ref.: #24926]	glucose ChEBI	+	fermentation
[Ref.: #24926]	glycerol ChEBI	+	fermentation
[Ref.: #24926]	glycine ChEBI	+	fermentation
[Ref.: #24926]	lactose ChEBI	+	fermentation
[Ref.: #24926]	maltose ChEBI	+	fermentation
[Ref.: #24926]	mannitol ChEBI	+	fermentation
[Ref.: #24926]	mannose ChEBI	+	fermentation
[Ref.: #24926]	nitrate ChEBI	+	electron acceptor
[Ref.: #24926]	nitrite ChEBI	-	electron acceptor
[Ref.: #24926]	peptone	+	fermentation
[Ref.: #24926]	starch ChEBI	+	fermentation
[Ref.: #24926]	sucrose ChEBI	+	fermentation
[Ref.: #24926]	sulfate ChEBI	-	electron acceptor
[Ref.: #24926]	thiosulfate ChEBI	-	electron acceptor
[Ref.: #24926]	trehalose ChEBI	+	fermentation
[Ref.: #24926]	trimethylamine ChEBI	+	fermentation
	Only first 10 entries are displayed. Click here to see all.

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #24926]		catalase	-	1.11.1.6
[Ref.: #24926]		cytochrome oxidase	-	1.9.3.1

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #24432]	Sample type/isolated from	deep subsurface groundwater
[Ref.: #24432]	Geographic location (country and/or sea, region)	Illinois, Decatur
[Ref.: #24432]	Country	USA
[Ref.: #24432]	Country ISO 3 Code	USA
[Ref.: #24432]	Continent	North America

[Ref.: #24926]	Sample type/isolated from	1.7 km depth groundwater
[Ref.: #24926]	Enrichment culture	iron-reducing enrichment culture
[Ref.: #24926]	Enrichment culture composition	10 mM Fe(III)-citrate as the electron acceptor and a mixture of fatty acids (5 mM each of acetate, lactate, pyruvate and formate) plus H2 (5 ml per 25 ml serum tube) as electron donors
[Ref.: #24926]	Enrichment culture duration	>180 days

[Ref.: #67770]	Sample type/isolated from	Groundwater at a depth of 1.7 km from the Mt. Simon Sandstone of the Illinois Basin
[Ref.: #67770]	Geographic location (country and/or sea, region)	IL
[Ref.: #67770]	Country	USA
[Ref.: #67770]	Country ISO 3 Code	USA
[Ref.: #67770]	Continent	North America
* marker position based on {}

Isolation sources categories

#Environmental

#Aquatic

#Groundwater

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #24432]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #24926]	Orenia metallireducens strain Z6 16S ribosomal RNA gene, partial sequence	KP898732	1495	GenBank	1413210 tax ID
[Ref.: #24432]	Tepidibacillus decaturensis strain Z9 16S ribosomal RNA gene, partial sequence	KP898733	1512	ENA	1413211 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Tepidibacillus decaturensis Z9	2675903671	draft	JGI IMG/M	1413211 tax ID	*
[Ref.: #67770]	Tepidibacillus decaturensis Z9	GCA_001561915	scaffold	GenBank	1413211 tax ID

[Ref.: #24926]	GC-content	36.1 mol%
[Ref.: #24432]	GC-content	36.1 mol%	sequence analysis
[Ref.: #67770]	GC-content	36.1 mol%	genome sequence analysis

Availability in culture collections External links

[Ref.: #24432]

Culture collection no.

DSM 103037, ATCC BAA 2644, JCM 31418

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Tepidibacillus decaturensis sp. nov., a microaerophilic, moderately thermophilic iron-reducing bacterium isolated from 1.7 km depth groundwater.	Dong Y, Sanford RA, Boyanov MI, Kemner KM, Flynn TM, O'Loughlin EJ, Locke RA, Weber JR, Egan SM, Fouke BW	Int J Syst Evol Microbiol	10.1099/ijsem.0.001295	2016	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#24432

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 103037

#24926

Y. S. Dong, Robert A.,Boyanov, Maxim I.,Kemner, Kenneth M.,Flynn, Theodore M.,O'Loughlin, Edward J.,Locke, Randall A.,Weber, Joseph R.,Egan, Sheila M.,Fouke, Bruce W.: Tepidibacillus decaturensis sp. nov., a microaerophilic, moderately thermophilic iron-reducing bacterium isolated from 1.7 km depth groundwater. IJSEM 66: 3964 - 3971 2016 ( DOI 10.1099/ijsem.0.001295 , PubMed 27406851 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

* These data were automatically processed and therefore are not curated

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