Name: Corynebacterium pollutisoli VDS11
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 132609

Type strain:

Species: Corynebacterium pollutisoli

Strain Designation: VDS11

Culture col. no.: DSM 100104, KCTC 39687, MCC 2722

Strain history: <- L. Rup, Univ. Delhi, Dept. Zoology, India; VDS11 <- V. Negi

NCBI tax ID(s): 1610489 (species)

Section

Corynebacterium pollutisoli VDS11 is an aerobe, mesophilic, Gram-positive bacterium that forms circular colonies and was isolated from soil from a hexachlorocyclohexane dumpsite.

colony-forming
Gram-positive
ovoid-shaped
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Mycobacteriales*
	Family	*Corynebacteriaceae*
	Genus	*Corynebacterium*
	Species	**Corynebacterium pollutisoli**
	Full Scientific Name (LPSN)	Corynebacterium pollutisoli Negi et al. 2016

Information on morphological and physiological properties Morphology

[Ref.: #43107]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #43107]	Cell length	0.75 µm

[Ref.: #43107]	Cell width	0.51 µm

[Ref.: #43107]	Cell shape	ovoid-shaped

[Ref.: #43107]	Motility	no
[Ref.: #69480]	Motility	no Confidence in %94.495

[Ref.: #43107]	Colony color	pale yellow
[Ref.: #43107]	Colony shape	circular
[Ref.: #43107]	Cultivation medium used	LB agar

Information on culture and growth conditions Culture and growth conditions

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Culture medium

LB (Luria-Bertani) MEDIUM

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Culture medium growth

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Culture medium

trypticase soy broth agar (TSBA)

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Culture medium growth

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Culture medium

nutritient agar (NA)

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Culture medium growth

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Culture medium

Reasoner's 2A agar (R2A)

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Culture medium growth

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Culture medium

tryptone soya yeast extract agar

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Culture medium growth

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Culture medium

xanthine agar

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Culture medium growth

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Culture medium

hypoxanthine agar

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Culture medium growth

	Temperatures	Kind of temperature		Temperature
[Ref.: #43107]			optimum	28 °C
[Ref.: #43107]			growth	10-45 °C

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Temperature range

mesophilic

	pH	Kind of pH		pH
[Ref.: #43107]			optimum	8
[Ref.: #43107]			growth	6-10

Information on physiology and metabolism Physiology and metabolism

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Oxygen tolerance

aerobe

[Ref.: #43107]	Ability of spore formation	no
[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.989

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43107]		NaCl		growth	0-10	%(w/v)
[Ref.: #43107]		NaCl		optimum	6	%(w/v)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43107]	gelatin ChEBI	+	hydrolysis
[Ref.: #43107]	glucose ChEBI	+	fermentation
[Ref.: #43107]	glycogen ChEBI	-	fermentation
[Ref.: #43107]	lactose ChEBI	-	fermentation
[Ref.: #43107]	maltose ChEBI	-	fermentation
[Ref.: #43107]	mannitol ChEBI	-	fermentation
[Ref.: #43107]	nitrate ChEBI	+	reduction
[Ref.: #43107]	ribose ChEBI	+	fermentation
[Ref.: #43107]	sucrose ChEBI	-	fermentation
[Ref.: #43107]	xylose ChEBI	-	fermentation

	Metabolite	Antibiotic sensitivity	Concentration		Antibiotic resistance	Concentration
[Ref.: #43107]	amikacin ChEBI	yes	30	µg (disc)
[Ref.: #43107]	ampicillin ChEBI				yes	10	µg (disc)
[Ref.: #43107]	chloramphenicol ChEBI				yes	30	µg (disc)
[Ref.: #43107]	ciprofloxacin ChEBI	yes	5	µg (disc)
[Ref.: #43107]	gentamicin ChEBI	yes	10	µg (disc)
[Ref.: #43107]	kanamycin ChEBI	yes	30	µg (disc)
[Ref.: #43107]	nalidixic acid ChEBI				yes	30	µg (disc)
[Ref.: #43107]	oxytetracycline ChEBI	yes	30	µg (disc)
[Ref.: #43107]	penicillin g ChEBI	yes	10	µg (disc)
[Ref.: #43107]	polymyxin b ChEBI				yes	300	µg (disc)
[Ref.: #43107]	rifampicin ChEBI	yes	5	µg (disc)
[Ref.: #43107]	tetracycline ChEBI	yes	30	µg (disc)
[Ref.: #43107]	vancomycin ChEBI	yes	30	µg (disc)
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #43107]	alkaline phosphatase	+	3.1.3.1
[Ref.: #43107]	alpha-glucosidase	-	3.2.1.20
[Ref.: #43107]	beta-galactosidase	-	3.2.1.23
[Ref.: #43107]	beta-glucosidase	-	3.2.1.21
[Ref.: #43107]	beta-glucuronidase	+	3.2.1.31
[Ref.: #43107]	catalase	-	1.11.1.6
[Ref.: #43107]	cytochrome oxidase	-	1.9.3.1
[Ref.: #43107]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #43107]	pyrazinamidase	+	3.5.1.B15
[Ref.: #43107]	pyrrolidonyl arylamidase	-	3.4.19.3
[Ref.: #43107]	urease	-	3.5.1.5
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #43107]

Fatty acid	Percentage
C18:1ω9c	52.3
C16:0	28.3
C20:0	3
C16:1ω7c / C16:1ω6c	2.9

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #24398]	Sample type/isolated from	soil from a hexachlorocyclohexane dumpsite
[Ref.: #24398]	Geographic location (country and/or sea, region)	Uttar Pradesh, Lucknow, Ummari Village (27° 00' 24.7'' N, 81° 08' 57.8'' E)
[Ref.: #24398]	Country	India
[Ref.: #24398]	Country ISO 3 Code	IND
[Ref.: #24398]	Continent	Asia
[Ref.: #24398]	Geographic location	27.0069°/81.1494°

[Ref.: #43107]	Sample type/isolated from	dumpsite soil contaminated with hexachlorocyclohexane (HCH)
[Ref.: #43107]	Geographic location (country and/or sea, region)	Ummari village, Lucknow
[Ref.: #43107]	Country	India
[Ref.: #43107]	Country ISO 3 Code	IND
[Ref.: #43107]	Continent	Asia
[Ref.: #43107]	Geographic location	27.0000°/81.0000°
[Ref.: #43107]	Enrichment culture	Luria-Bertani (LB) agar
[Ref.: #43107]	Enrichment culture composition	supplemented with nyastin and streptomycin
[Ref.: #43107]	Enrichment culture duration	2 days
[Ref.: #43107]	Enrichment culture temperature	28 ̊C
[Ref.: #43107]	Isolation/enrichment procedure	serial dilution plating method
* marker position based on {}

Isolation sources categories

#Engineered	#Waste	-
#Environmental	#Terrestrial	#Soil

What are isolation sources categories?

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #24398]	Corynebacterium pollutisoli strain VDS11 16S ribosomal RNA gene, partial sequence	KP276149	1403	ENA	1610489 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Corynebacterium pollutisoli VDS11	GCA_900177745	scaffold	GenBank	1610489 tax ID	*

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GC-content

50.6 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Corynebacterium pollutisoli VDS11 NCBI: GCA_900177745.1
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Corynebacterium pollutisoli VDS11 NCBI: GCA_900177745.1
[Ref.: #69480]	motile	no	93.478	yes
[Ref.: #69480]	flagellated	no	97.823	yes
[Ref.: #69480]	gram-positive	yes	92.621	yes
[Ref.: #69480]	anaerobic	no	98.819	no
[Ref.: #69480]	aerobic	yes	70.564	yes
[Ref.: #69480]	halophile	yes	79.947	yes
[Ref.: #69480]	spore-forming	no	91.435	yes
[Ref.: #69480]	thermophile	no	98.622	yes
[Ref.: #69480]	glucose-util	yes	89.308	no
[Ref.: #69480]	glucose-ferment	yes	52.937	no

Availability in culture collections External links

[Ref.: #24398]

Culture collection no.

DSM 100104, KCTC 39687, MCC 2722

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Corynebacteriumpollutisoli sp. nov., isolated from hexachlorocyclohexane-contaminated soil.	Negi V, Singh Y, Schumann P, Lal R	Int J Syst Evol Microbiol	10.1099/ijsem.0.001228	2016	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#24398

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 100104

#43107

Vivek Negi, Yogendra Singh, Peter Schumann, Rup Lal: Corynebacterium pollutisoli sp. nov., isolated from hexachlorocyclohexane-contaminated soil. IJSEM 66: 3531 - 3537 2016 ( DOI 10.1099/ijsem.0.001228 , PubMed 27267453 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

* These data were automatically processed and therefore are not curated

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