Name: Pyrococcus kukulkanii Souchothèque de Bretagne BG1337
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 132578

Type strain:

Species: Pyrococcus kukulkanii

Strain Designation: Souchothèque de Bretagne BG1337

Culture col. no.: DSM 101590, NCB 100

Strain history: <- A. Godfroy, IFREMER; NCB100T <- C. Nolwenn {2010}

NCBI tax ID(s): 1609559 (species)

SI-ID 406939

DSM 101590

Section

Pyrococcus kukulkanii Souchothèque de Bretagne BG1337 is an obligate anaerobe, spore-forming, hyperthermophilic archaeon that was isolated from flange of an active deep-sea hydrothermal vent chimney at the depth of 1997 m.

coccus-shaped
motile
spore-forming
hyperthermophilic
obligate anaerobe
Archaea
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Archaea"
	Phylum	*Methanobacteriota*
	Class	*Thermococci*
	Order	*Thermococcales*
	Family	*Thermococcaceae*
	Genus	*Pyrococcus*
	Species	**Pyrococcus kukulkanii**
	Full Scientific Name (LPSN)	Pyrococcus kukulkanii Callac et al. 2016

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	negative Confidence in %99.974

[Ref.: #43080]	Cell width	1 µm

[Ref.: #43080]	Cell shape	coccus-shaped

[Ref.: #43080]	Motility	yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #24367]

Culture medium

PYROCOCCUS MEDIUM (DSMZ Medium 377)

[Ref.: #24367]

Culture medium growth

[Ref.: #24367]

Culture medium link

Medium recipe at MediaDive

[Ref.: #24367]

Culture medium composition

expand / minimize

	Kind of temperature		Temperature
[Ref.: #24367]		growth	100 °C
[Ref.: #43080]	no	growth	70-112 °C
[Ref.: #43080]		optimum	105 °C

[Ref.: #24367]	Temperature range	hyperthermophilic
[Ref.: #43080]	Temperature range	hyperthermophilic
[Ref.: #69480]	Temperature range	thermophilic Confidence in %100

	pH	Kind of pH		pH
[Ref.: #43080]			optimum	7
[Ref.: #43080]			growth	3.5-8.5

Information on physiology and metabolism Physiology and metabolism

[Ref.: #43080]	Oxygen tolerance	obligate anaerobe
[Ref.: #69480]	Oxygen tolerance	anaerobe Confidence in %99.507

[Ref.: #69481]	Ability of spore formation	yes Confidence in %99
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.792

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43080]		NaCl		growth	1.5-7	%(w/v)
[Ref.: #43080]		NaCl		optimum	2.5-3	%(w/v)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43080]	acetate ChEBI	-	carbon source
[Ref.: #43080]	casamino acids	-	carbon source
[Ref.: #43080]	casein	-	carbon source
[Ref.: #43080]	cellobiose ChEBI	-	carbon source
[Ref.: #43080]	chitin ChEBI	-	carbon source
[Ref.: #43080]	elemental sulfur ChEBI	+	required for growth
[Ref.: #43080]	elemental sulfur ChEBI	+	reduction
[Ref.: #43080]	ethanol ChEBI	-	carbon source
[Ref.: #43080]	ferric iron ChEBI	-	electron acceptor
[Ref.: #43080]	formate ChEBI	-	carbon source
[Ref.: #43080]	galactose ChEBI	-	carbon source
[Ref.: #43080]	gelatin ChEBI	+	nitrogen source
[Ref.: #43080]	glucose ChEBI	-	carbon source
[Ref.: #43080]	glycerol ChEBI	-	carbon source
[Ref.: #43080]	glycogen ChEBI	+	carbon source
[Ref.: #43080]	L-cystine ChEBI	-	electron acceptor
[Ref.: #43080]	maltose ChEBI	-	carbon source
[Ref.: #43080]	nitrate ChEBI	-	electron acceptor
[Ref.: #43080]	nitrate ChEBI	+	nitrogen source
[Ref.: #43080]	peptone	+	carbon source
[Ref.: #43080]	peptone	+	nitrogen source
[Ref.: #43080]	pyruvate ChEBI	-	carbon source
[Ref.: #43080]	starch ChEBI	+	carbon source
[Ref.: #43080]	succinate ChEBI	-	carbon source
[Ref.: #43080]	sucrose ChEBI	-	carbon source
[Ref.: #43080]	sulfate ChEBI	-	electron acceptor
[Ref.: #43080]	sulfite ChEBI	-	electron acceptor
[Ref.: #43080]	thiosulfate ChEBI	-	electron acceptor
[Ref.: #43080]	tryptone	+	carbon source
[Ref.: #43080]	tryptone	+	nitrogen source
[Ref.: #43080]	urea ChEBI	+	nitrogen source
[Ref.: #43080]	yeast extract	+	carbon source
[Ref.: #43080]	yeast extract	+	nitrogen source
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #43080]	carbon dioxide ChEBI	yes
[Ref.: #43080]	dihydrogen ChEBI	yes
[Ref.: #43080]	hydrogen sulfide ChEBI	yes

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #24367]	Sample type/isolated from	flange of an active deep-sea hydrothermal vent chimney at the depth of 1997 m
[Ref.: #24367]	Geographic location (country and/or sea, region)	Gulf of California, Guaymas Basin at the Rebecca's Roost site
[Ref.: #24367]	Country	Mexico
[Ref.: #24367]	Country ISO 3 Code	MEX
[Ref.: #24367]	Continent	North America
[Ref.: #24367]	Geographic location	27.0106°/-111.4070°

[Ref.: #43080]	Sample type/isolated from	flange of an active deep-sea hydrothermal chimney at the depth of 1997 m
[Ref.: #43080]	Geographic location (country and/or sea, region)	Rebecca's Roost, Guaymas Basin, Gulf of California, Mexico
[Ref.: #43080]	Country	Mexico
[Ref.: #43080]	Country ISO 3 Code	MEX
[Ref.: #43080]	Continent	North America
[Ref.: #43080]	Geographic location	27.0000°/-111.0000°
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Hydrothermal vent
#Environmental	#Aquatic	#Marine
#Condition	#Thermophilic (>45°C)	-

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #24367]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Pyrococcus kukulkanii NCB100	GCA_001577775	complete	GenBank	1609559 tax ID	*
[Ref.: #66792]	Pyrococcus kukulkanii NCB100	2687453501	complete	JGI IMG/M	1609559 tax ID	*
[Ref.: #66792]	Pyrococcus sp. NCB100	1609559.3	complete	PATRIC	1609559 tax ID	*

[Ref.: #24367]

GC-content

41.3 mol%

thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Pyrococcus kukulkanii NCB100 NCBI: GCA_001577775.1
[Ref.: #69481]	spore-forming	yes	99	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Pyrococcus kukulkanii NCB100 NCBI: GCA_001577775.1
[Ref.: #69480]	flagellated	no	93.825	no
[Ref.: #69480]	gram-positive	no	95.437	no
[Ref.: #69480]	anaerobic	yes	99.342	no
[Ref.: #69480]	aerobic	no	97.749	yes
[Ref.: #69480]	halophile	no	68.871	no
[Ref.: #69480]	spore-forming	no	95.274	no
[Ref.: #69480]	thermophile	yes	100	no
[Ref.: #69480]	glucose-util	yes	79.378	no
[Ref.: #69480]	motile	no	70.343	no
[Ref.: #69480]	glucose-ferment	no	56.599	no

Availability in culture collections External links

[Ref.: #24367]

Culture collection no.

DSM 101590, NCB 100

[Ref.: #90895]

SI-ID 406939

Literature:

Topic	Title	Authors	Journal	DOI	Year
Genetics	Complete Genome Sequence of the Hyperthermophilic Piezophilic Archaeon Pyrococcus kukulkanii NCB100 Isolated from the Rebecca's Roost Hydrothermal Vent in the Guaymas Basin.	Oger PM, Callac N, Oger-Desfeux C, Hughes S, Gillet B, Jebbar M, Godfroy A	Genome Announc	10.1128/genomeA.01667-16	2017	*
Phylogeny	Pyrococcus kukulkanii sp. nov., a hyperthermophilic, piezophilic archaeon isolated from a deep-sea hydrothermal vent.	Callac N, Oger P, Lesongeur F, Rattray JE, Vannier P, Michoud G, Beauverger M, Gayet N, Rouxel O, Jebbar M, Godfroy A	Int J Syst Evol Microbiol	10.1099/ijsem.0.001160	2016	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#24367

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 101590

#43080

Nolwenn Callac, Philippe Oger, Françoise Lesongeur, Jayne E. Rattray, Pauline Vannier, Grégoire Michoud, Mickael Beauverger, Nicolas Gayet, Olivier Rouxel, Mohamed Jebbar and Anne Godfroy: Pyrococcus kukulkanii sp. nov., a hyperthermophilic, piezophilic archaeon isolated from a deep-sea hydrothermal vent. IJSEM 66: 3142 - 3149 2016 ( DOI 10.1099/ijsem.0.001160 , PubMed 27189596 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#90895

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID406939.1 )

* These data were automatically processed and therefore are not curated

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Pyrococcus kukulkanii NCB100

Pyrococcus kukulkanii NCB100

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