Name: Pseudomonas weihenstephanensis WS 4993
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 132438

Type strain:

Species: Pseudomonas weihenstephanensis

Strain Designation: WS 4993

Culture col. no.: DSM 29166, LMG 28437

Strain history: <- M. Wenning, (ZIEL) TU Munich, Microbiology, Freising-Weihenstephan, Germany; WS4993 <- M. Freiherr von Neubeck, ZIEL

NCBI tax ID(s): 1608994 (species)

SI-ID 396473

LMG 28437, DSM 29166

Other strains from Pseudomonas weihenstephanensis

P. weihenstephanensis WS 4994, DSM 29140, LMG 28438

Section

Pseudomonas weihenstephanensis WS 4993 is an aerobe, Gram-negative, motile bacterium that forms circular colonies and was isolated from raw cow’s milk.

colony-forming
Gram-negative
motile
rod-shaped
aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Pseudomonadales*
	Family	*Pseudomonadaceae*
	Genus	*Pseudomonas*
	Species	**Pseudomonas weihenstephanensis**
	Full Scientific Name (LPSN)	Pseudomonas weihenstephanensis von Neubeck et al. 2016

Information on morphological and physiological properties Morphology

[Ref.: #43746]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %99.996

[Ref.: #43746]	Cell length	2.1 µm

[Ref.: #43746]	Cell width	0.75 µm

[Ref.: #43746]	Cell shape	rod-shaped

[Ref.: #43746]	Motility	yes
[Ref.: #69480]	Motility	yes Confidence in %96.741

[Ref.: #43746]	Type of hemolysis	gamma
[Ref.: #43746]	Colony size	0.5-1 mm
[Ref.: #43746]	Colony color	Beige
[Ref.: #43746]	Colony shape	circular
[Ref.: #43746]	Incubation period	1 day
[Ref.: #43746]	Cultivation medium used	TSG

Multimedia content

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Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

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Culture medium

Cetrimide agar

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Culture medium growth

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Culture medium

Reasoner's 2A agar (R2A)

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Culture medium growth

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Culture medium

TSG

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Culture medium growth

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Culture medium

REACTIVATION WITH LIQUID MEDIUM 1 (DSMZ Medium 1a)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

expand / minimize

		Temperature
[Ref.: #24227]	growth	28 °C
[Ref.: #43746]	growth	4-33 °C
[Ref.: #43746]	optimum	23-27 °C

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Temperature range

mesophilic

	pH	Kind of pH		pH
[Ref.: #43746]			growth	5-8

Information on physiology and metabolism Physiology and metabolism

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Oxygen tolerance

aerobe

[Ref.: #43746]	Ability of spore formation	no
[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.953

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43746]		NaCl		growth	5	%(w/v)

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Observation

Fluorescent on King B agar

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43746]	amygdalin ChEBI	-	assimilation
[Ref.: #43746]	arbutin ChEBI	-	assimilation
[Ref.: #43746]	cellobiose ChEBI	-	assimilation
[Ref.: #43746]	D-arabinose ChEBI	-	assimilation
[Ref.: #43746]	D-arabitol ChEBI	-	assimilation
[Ref.: #43746]	D-fructose ChEBI	+	assimilation
[Ref.: #43746]	D-fucose ChEBI	-	assimilation
[Ref.: #43746]	D-galactose ChEBI	-	assimilation
[Ref.: #43746]	D-glucose ChEBI	+	assimilation
[Ref.: #43746]	D-lyxose ChEBI	-	assimilation
[Ref.: #43746]	D-mannitol ChEBI	-	assimilation
[Ref.: #43746]	D-mannose ChEBI	+	assimilation
[Ref.: #43746]	D-ribose ChEBI	+	assimilation
[Ref.: #43746]	D-sorbitol ChEBI	-	assimilation
[Ref.: #43746]	D-tagatose ChEBI	-	assimilation
[Ref.: #43746]	D-xylose ChEBI	+	assimilation
[Ref.: #43746]	erythritol ChEBI	-	assimilation
[Ref.: #43746]	galactitol ChEBI	-	assimilation
[Ref.: #43746]	gelatin ChEBI	-	hydrolysis
[Ref.: #43746]	gentiobiose ChEBI	-	assimilation
[Ref.: #43746]	glycerol ChEBI	+	assimilation
[Ref.: #43746]	glycogen ChEBI	-	assimilation
[Ref.: #43746]	L-arabinose ChEBI	-	assimilation
[Ref.: #43746]	L-arabitol ChEBI	-	assimilation
[Ref.: #43746]	L-fucose ChEBI	-	assimilation
[Ref.: #43746]	L-rhamnose ChEBI	-	assimilation
[Ref.: #43746]	L-sorbose ChEBI	-	assimilation
[Ref.: #43746]	L-xylose ChEBI	-	assimilation
[Ref.: #43746]	lactose ChEBI	-	assimilation
[Ref.: #43746]	maltose ChEBI	-	assimilation
[Ref.: #43746]	melezitose ChEBI	-	assimilation
[Ref.: #43746]	melibiose ChEBI	-	assimilation
[Ref.: #43746]	methyl alpha-D-mannoside ChEBI	-	assimilation
[Ref.: #43746]	methyl beta-D-xylopyranoside ChEBI	-	assimilation
[Ref.: #43746]	methyl D-glucoside ChEBI	-	assimilation
[Ref.: #43746]	myo-inositol ChEBI	+	assimilation
[Ref.: #43746]	N-acetylglucosamine ChEBI	-	assimilation
[Ref.: #43746]	nitrate ChEBI	-	reduction
[Ref.: #43746]	nitrite ChEBI	-	reduction
[Ref.: #43746]	potassium gluconate ChEBI	+	assimilation
[Ref.: #43746]	raffinose ChEBI	-	assimilation
[Ref.: #43746]	ribitol ChEBI	-	assimilation
[Ref.: #43746]	salicin ChEBI	-	assimilation
[Ref.: #43746]	starch ChEBI	-	hydrolysis
[Ref.: #43746]	sucrose ChEBI	-	assimilation
[Ref.: #43746]	trehalose ChEBI	+	assimilation
[Ref.: #43746]	turanose ChEBI	-	assimilation
[Ref.: #43746]	xylitol ChEBI	-	assimilation
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #43746]		hydrogen sulfide ChEBI	no
[Ref.: #43746]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #43746]	beta-galactosidase	-	3.2.1.23
[Ref.: #43746]	catalase	+	1.11.1.6
[Ref.: #43746]	cytochrome oxidase	+	1.9.3.1

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #24227]	Sample type/isolated from	raw cow’s milk
[Ref.: #24227]	Country	Germany
[Ref.: #24227]	Country ISO 3 Code	DEU
[Ref.: #24227]	Continent	Europe

[Ref.: #43746]	Sample type/isolated from	Raw cow's milk
* marker position based on {}

Isolation sources categories

#Host	#Mammals	#Bovinae (Cow, Cattle)
#Host Body Product	#Fluids	#Milk

What are isolation sources categories?

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Global distribution of 16S sequence KP738720 (>99% sequence identity) for Pseudomonas from Microbeatlas

Aquatic Samples	830
Soil Samples	460
Animal Samples	1081
Plant Samples	571
Total Samples	2942

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

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Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #24227]	Pseudomonas weihenstephanensis strain DSM 29166 16S ribosomal RNA gene, partial sequence	KP738720	1525	ENA	1608994 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Pseudomonas weihenstephanensis DSM 29166	GCA_001043055	contig	GenBank	1608994 tax ID	*
[Ref.: #66792]	Pseudomonas sp. DSM 29166	1608994.3	wgs	PATRIC	1608994 tax ID	*
[Ref.: #66792]	Pseudomonas weihenstephanensis DSM 29166	2806310669	draft	JGI IMG/M	1608994 tax ID	*

[Ref.: #43746]	GC-content	57.3 mol%	genome sequence analysis
[Ref.: #24227]	GC-content	57.3 mol%	sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Pseudomonas sp. DSM 29166 PATRIC: 1608994.5
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Pseudomonas sp. DSM 29166 PATRIC: 1608994.3
[Ref.: #69480]	motile	yes	91.457	yes
[Ref.: #69480]	gram-positive	no	98.632	yes
[Ref.: #69480]	anaerobic	no	97.78	no
[Ref.: #69480]	aerobic	yes	91.671	no
[Ref.: #69480]	halophile	no	87.454	no
[Ref.: #69480]	spore-forming	no	95.799	no
[Ref.: #69480]	thermophile	no	99.743	yes
[Ref.: #69480]	glucose-util	yes	88.241	no
[Ref.: #69480]	flagellated	yes	87.711	no
[Ref.: #69480]	glucose-ferment	no	85.547	no

Availability in culture collections External links

[Ref.: #24227]

Culture collection no.

DSM 29166, LMG 28437

[Ref.: #90777]

SI-ID 396473

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Pseudomonas paraversuta sp. nov. isolated from refrigerated dry-aged beef.	Lick S, Wibberg D, Winkler A, Blom J, Grimmler C, Goesmann A, Kalinowski J, Krockel L	Int J Syst Evol Microbiol	10.1099/ijsem.0.004822	2021	*
Phylogeny	Pseudomonas versuta sp. nov., isolated from Antarctic soil.	See-Too WS, Salazar S, Ee R, Convey P, Chan KG, Peix A	Syst Appl Microbiol	10.1016/j.syapm.2017.03.002	2017	*
Phylogeny	Pseudomonas helleri sp. nov. and Pseudomonas weihenstephanensis sp. nov., isolated from raw cow's milk.	von Neubeck M, Huptas C, Gluck C, Krewinkel M, Stoeckel M, Stressler T, Fischer L, Hinrichs J, Scherer S, Wenning M	Int J Syst Evol Microbiol	10.1099/ijsem.0.000852	2015	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#24227

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 29166

#43746

M. von Neubeck, C. Huptas, C. Glück, M. Krewinkel, M. Stoeckel, T. Stressler, L. Fischer, J. Hinrichs, S. Scherer, M. Wenning: Pseudomonas helleri sp. nov. and Pseudomonas weihenstephanensis sp. nov., isolated from raw cow's milk. IJSEM 66: 1163 - 1173 2016 ( DOI 10.1099/ijsem.0.000852 , PubMed 26675012 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#90777

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID396473.1 )

* These data were automatically processed and therefore are not curated

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Pseudomonas sp. DSM 29166

Pseudomonas sp. DSM 29166

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