Name: Pseudomonas helleri WS 4917
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 132436

Type strain:

Species: Pseudomonas helleri

Strain Designation: WS 4917

Culture col. no.: DSM 29165, LMG 28433

Strain history: <- M. Wenning, (ZIEL), TU Munich, Microbiology, Germany; WS4917 <- M. Freiherr von Neubeck, ZIEL

NCBI tax ID(s): 1608996 (species)

SI-ID 396467

LMG 28433, DSM 29165

Special Collections

Other strains from Pseudomonas helleri

Section

Pseudomonas helleri WS 4917 is an aerobe, Gram-negative, motile bacterium that forms circular colonies and was isolated from raw cow’s milk.

colony-forming
Gram-negative
motile
rod-shaped
aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Pseudomonadales*
	Family	*Pseudomonadaceae*
	Genus	*Pseudomonas*
	Species	**Pseudomonas helleri**
	Full Scientific Name (LPSN)	Pseudomonas helleri von Neubeck et al. 2016

Information on morphological and physiological properties Morphology

[Ref.: #43746]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %99.5

[Ref.: #43746]	Cell length	3.1 µm

[Ref.: #43746]	Cell width	0.75 µm

[Ref.: #43746]	Cell shape	rod-shaped

[Ref.: #43746]	Motility	yes
[Ref.: #69480]	Motility	yes Confidence in %90.178

[Ref.: #43746]	Type of hemolysis	gamma
[Ref.: #43746]	Colony size	0.5-1 mm
[Ref.: #43746]	Colony color	Beige
[Ref.: #43746]	Colony shape	circular
[Ref.: #43746]	Incubation period	1 day
[Ref.: #43746]	Cultivation medium used	TSG

Multimedia content

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Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

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Culture medium

Cetrimide agar

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Culture medium growth

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Culture medium

Reasoner's 2A agar (R2A)

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Culture medium growth

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Culture medium

TSG

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Culture medium growth

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Culture medium

REACTIVATION WITH LIQUID MEDIUM 1 (DSMZ Medium 1a)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

expand / minimize

		Temperature
[Ref.: #24225]	growth	28 °C
[Ref.: #43746]	growth	4-31 °C
[Ref.: #43746]	optimum	25-29 °C

	pH	Kind of pH		pH
[Ref.: #43746]			growth	5-8

Information on physiology and metabolism Physiology and metabolism

[Ref.: #43746]	Oxygen tolerance	aerobe
[Ref.: #69480]	Oxygen tolerance	aerobe Confidence in %90.033

[Ref.: #43746]	Ability of spore formation	no
[Ref.: #69481]	Ability of spore formation	no Confidence in %100

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43746]		NaCl		growth	6	%(w/v)

[Ref.: #43746]	Observation	Fluorescent on King B agar
[Ref.: #43746]	Observation	No pyocyanin is formed on King A agar

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43746]	amygdalin ChEBI	-	assimilation
[Ref.: #43746]	arbutin ChEBI	-	assimilation
[Ref.: #43746]	cellobiose ChEBI	-	assimilation
[Ref.: #43746]	D-arabinose ChEBI	+	assimilation
[Ref.: #43746]	D-arabitol ChEBI	+/-	assimilation
[Ref.: #43746]	D-fructose ChEBI	+	assimilation
[Ref.: #43746]	D-fucose ChEBI	-	assimilation
[Ref.: #43746]	D-galactose ChEBI	+	assimilation
[Ref.: #43746]	D-glucose ChEBI	+	assimilation
[Ref.: #43746]	D-lyxose ChEBI	-	assimilation
[Ref.: #43746]	D-mannitol ChEBI	+/-	assimilation
[Ref.: #43746]	D-mannose ChEBI	+	assimilation
[Ref.: #43746]	D-ribose ChEBI	+	assimilation
[Ref.: #43746]	D-sorbitol ChEBI	-	assimilation
[Ref.: #43746]	D-tagatose ChEBI	-	assimilation
[Ref.: #43746]	D-xylose ChEBI	+	assimilation
[Ref.: #43746]	erythritol ChEBI	-	assimilation
[Ref.: #43746]	galactitol ChEBI	-	assimilation
[Ref.: #43746]	gelatin ChEBI	-	hydrolysis
[Ref.: #43746]	gentiobiose ChEBI	-	assimilation
[Ref.: #43746]	glycerol ChEBI	+	assimilation
[Ref.: #43746]	glycogen ChEBI	-	assimilation
[Ref.: #43746]	L-arabinose ChEBI	-	assimilation
[Ref.: #43746]	L-arabitol ChEBI	-	assimilation
[Ref.: #43746]	L-fucose ChEBI	+	assimilation
[Ref.: #43746]	L-rhamnose ChEBI	-	assimilation
[Ref.: #43746]	L-sorbose ChEBI	-	assimilation
[Ref.: #43746]	L-xylose ChEBI	-	assimilation
[Ref.: #43746]	lactose ChEBI	-	assimilation
[Ref.: #43746]	maltose ChEBI	-	assimilation
[Ref.: #43746]	melezitose ChEBI	-	assimilation
[Ref.: #43746]	melibiose ChEBI	-	assimilation
[Ref.: #43746]	methyl alpha-D-mannoside ChEBI	-	assimilation
[Ref.: #43746]	methyl beta-D-xylopyranoside ChEBI	-	assimilation
[Ref.: #43746]	methyl D-glucoside ChEBI	-	assimilation
[Ref.: #43746]	myo-inositol ChEBI	-	assimilation
[Ref.: #43746]	N-acetylglucosamine ChEBI	-	assimilation
[Ref.: #43746]	nitrate ChEBI	-	reduction
[Ref.: #43746]	nitrite ChEBI	-	reduction
[Ref.: #43746]	potassium gluconate ChEBI	+	assimilation
[Ref.: #43746]	raffinose ChEBI	-	assimilation
[Ref.: #43746]	ribitol ChEBI	-	assimilation
[Ref.: #43746]	salicin ChEBI	-	assimilation
[Ref.: #43746]	starch ChEBI	-	hydrolysis
[Ref.: #43746]	sucrose ChEBI	-	assimilation
[Ref.: #43746]	trehalose ChEBI	+/-	assimilation
[Ref.: #43746]	turanose ChEBI	-	assimilation
[Ref.: #43746]	xylitol ChEBI	-	assimilation
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #43746]		hydrogen sulfide ChEBI	no
[Ref.: #43746]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #43746]	beta-galactosidase	-	3.2.1.23
[Ref.: #43746]	catalase	+	1.11.1.6
[Ref.: #43746]	cytochrome oxidase	+	1.9.3.1

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #24225]	Sample type/isolated from	raw cow’s milk
[Ref.: #24225]	Geographic location (country and/or sea, region)	Southern Germany
[Ref.: #24225]	Country	Germany
[Ref.: #24225]	Country ISO 3 Code	DEU
[Ref.: #24225]	Continent	Europe

[Ref.: #43746]	Sample type/isolated from	Raw cow's milk
* marker position based on {}

Isolation sources categories

#Host	#Mammals	#Bovinae (Cow, Cattle)
#Host Body Product	#Fluids	#Milk

What are isolation sources categories?

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Global distribution of 16S sequence KP738715 (>99% sequence identity) for Pseudomonas from Microbeatlas

Aquatic Samples	830
Soil Samples	460
Animal Samples	1081
Plant Samples	571
Total Samples	2942

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

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Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #24225]	Pseudomonas helleri strain DSM 29165 16S ribosomal RNA gene, partial sequence	KP738715	1525	GenBank ENA	1608996 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Pseudomonas helleri DSM 29165	GCA_001043025	contig	GenBank ENA	1608996 tax ID	*
[Ref.: #66792]	Pseudomonas sp. DSM 29165	1608996.3	wgs	PATRIC	1608996 tax ID	*
[Ref.: #66792]	Pseudomonas helleri DSM 29165	2784132037	draft	JGI IMG/M	1608996 tax ID	*

[Ref.: #43746]	GC-content	58.1 mol%	genome sequence analysis
[Ref.: #24225]	GC-content	58.1 mol%	sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Pseudomonas sp. DSM 29165 PATRIC: 1608996.3
[Ref.: #69481]	spore-forming	spore-formingⓘ	no	100	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Pseudomonas sp. DSM 29165 PATRIC: 1608996.3
[Ref.: #69480]	gram-positive	gram-positiveⓘ	no	99.5	yes
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	99.051	yes
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	81.167	no
[Ref.: #69480]	aerobic	aerobicⓘ	yes	90.033	yes
[Ref.: #69480]	thermophilic	thermophileⓘ	no	97.5	yes
[Ref.: #69480]	flagellated	motile2+ⓘ	yes	90.178	no

Availability in culture collections External links

[Ref.: #24225]

Culture collection no.

DSM 29165, LMG 28433

[Ref.: #90775]

SI-ID 396467

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Pseudomonas helleri sp. nov. and Pseudomonas weihenstephanensis sp. nov., isolated from raw cow's milk.	von Neubeck M, Huptas C, Gluck C, Krewinkel M, Stoeckel M, Stressler T, Fischer L, Hinrichs J, Scherer S, Wenning M	Int J Syst Evol Microbiol	10.1099/ijsem.0.000852	2015	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#24225

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 29165

#43746

M. von Neubeck, C. Huptas, C. Glück, M. Krewinkel, M. Stoeckel, T. Stressler, L. Fischer, J. Hinrichs, S. Scherer, M. Wenning: Pseudomonas helleri sp. nov. and Pseudomonas weihenstephanensis sp. nov., isolated from raw cow's milk. IJSEM 66: 1163 - 1173 2016 ( DOI 10.1099/ijsem.0.000852 , PubMed 26675012 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#90775

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID396467.1 )

* These data were automatically processed and therefore are not curated

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Pseudomonas sp. DSM 29165

Pseudomonas sp. DSM 29165

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