Pseudomonas helleri (DSM 29165, LMG 28433, WS 4917)

Name: Pseudomonas helleri (DSM 29165, LMG 28433, WS 4917)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 132436

Type strain

Strain Designation WS 4917

Culture col. no. DSM 29165 LMG 28433 WS 4917

NCBI tax ID(s) 1608996

Special Collections DSMZ Literature strains

History DSMZ <- M. Wenning, (ZIEL), TU Munich, Microbiology, Germany; WS4917 <- M. Freiherr von Neubeck, ZIEL

Links

Pseudomonas helleri WS 4917 is an aerobe, Gram-negative, motile bacterium that forms circular colonies and was isolated from raw cow’s milk.

Gram-negative motile rod-shaped colony-forming aerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 396467

LMG 28433,DSM 29165

@ref 20215

Last LPSN update 2026-05-29 08:37:51

Domain Bacteria

Phylum Pseudomonadota

Class Gammaproteobacteria

Order Pseudomonadales

Family Pseudomonadaceae

Genus Pseudomonas

Species Pseudomonas helleri

Full scientific name Pseudomonas helleri von Neubeck et al. 2016

BacDive ID	Other strains from **Pseudomonas helleri** (1)	Type strain
132435	P. helleri WS 4995, DSM 29141, LMG 28434

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Confidence
43746	negative	3.1 µm	0.75 µm	rod-shaped
125438					90.178
125438	negative				99.5
125439	negative				97.937

Colony morphology

@ref	Type of hemolysis	Hemolysis ability	Colony size	Colony color	Colony shape	Incubation period	Medium used
43746	gamma	0	0.5-1 mm	Beige	circular	1 day	TSG

Multimedia

@ref:	24225
multimedia content:	DSM_29165.jpg
multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_29165.jpg
intellectual property rights:	© Leibniz-Institut DSMZ
manual_annotation:	1

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
43746	Cetrimide agar
43746	Reasoner's 2A agar (R2A)
43746	TSG
24225	REACTIVATION WITH LIQUID MEDIUM 1 (DSMZ Medium 1a)	Medium recipe at MediaDive	Name: REACTIVATION WITH LIQUID MEDIUM 1 (DSMZ Medium 1a) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
24225	positive	growth	28
43746	positive	growth	4-31
43746	positive	optimum	25-29

Culture pH

@ref	Ability	Type	PH
43746	positive	growth	5-8

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
43746	aerobe
125438	aerobe	90.033

Spore formation

@ref	Spore formation	Confidence
43746
125439		99.843

Halophily

@ref	Salt	Growth	Tested relation	Concentration
43746	NaCl	positive	growth	6 %(w/v)

Observation

@ref	Observation
43746	Fluorescent on King B agar
43746	No pyocyanin is formed on King A agar

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
43746	27613 ChEBI	amygdalin	-	assimilation
43746	18305 ChEBI	arbutin	-	assimilation
43746	17057 ChEBI	cellobiose	-	assimilation
43746	17108 ChEBI	D-arabinose	+	assimilation
43746	18333 ChEBI	D-arabitol	+/-	assimilation
43746	15824 ChEBI	D-fructose	+	assimilation
43746	28847 ChEBI	D-fucose	-	assimilation
43746	12936 ChEBI	D-galactose	+	assimilation
43746	17634 ChEBI	D-glucose	+	assimilation
43746	62318 ChEBI	D-lyxose	-	assimilation
43746	16899 ChEBI	D-mannitol	+/-	assimilation
43746	16024 ChEBI	D-mannose	+	assimilation
43746	16988 ChEBI	D-ribose	+	assimilation
43746	17924 ChEBI	D-sorbitol	-	assimilation
43746	16443 ChEBI	D-tagatose	-	assimilation
43746	65327 ChEBI	D-xylose	+	assimilation
43746	17113 ChEBI	erythritol	-	assimilation
43746	16813 ChEBI	galactitol	-	assimilation
43746	5291 ChEBI	gelatin	-	hydrolysis
43746	28066 ChEBI	gentiobiose	-	assimilation
43746	17754 ChEBI	glycerol	+	assimilation
43746	28087 ChEBI	glycogen	-	assimilation
43746	30849 ChEBI	L-arabinose	-	assimilation
43746	18403 ChEBI	L-arabitol	-	assimilation
43746	18287 ChEBI	L-fucose	+	assimilation
43746	62345 ChEBI	L-rhamnose	-	assimilation
43746	17266 ChEBI	L-sorbose	-	assimilation
43746	65328 ChEBI	L-xylose	-	assimilation
43746	17716 ChEBI	lactose	-	assimilation
43746	17306 ChEBI	maltose	-	assimilation
43746	6731 ChEBI	melezitose	-	assimilation
43746	28053 ChEBI	melibiose	-	assimilation
43746	43943 ChEBI	methyl alpha-D-mannoside	-	assimilation
43746	74863 ChEBI	methyl beta-D-xylopyranoside	-	assimilation
43746	37657 ChEBI	methyl D-glucoside	-	assimilation
43746	17268 ChEBI	myo-inositol	-	assimilation
43746	506227 ChEBI	N-acetylglucosamine	-	assimilation
43746	17632 ChEBI	nitrate	-	reduction
43746	16301 ChEBI	nitrite	-	reduction
43746	32032 ChEBI	potassium gluconate	+	assimilation
43746	16634 ChEBI	raffinose	-	assimilation
43746	15963 ChEBI	ribitol	-	assimilation
43746	17814 ChEBI	salicin	-	assimilation
43746	28017 ChEBI	starch	-	hydrolysis
43746	17992 ChEBI	sucrose	-	assimilation
43746	27082 ChEBI	trehalose	+/-	assimilation
43746	32528 ChEBI	turanose	-	assimilation
43746	17151 ChEBI	xylitol	-	assimilation

Metabolite production

@ref	Chebi-ID	Metabolite	Production
43746	16136 ChEBI	hydrogen sulfide
43746	35581 ChEBI	indole

Enzymes

@ref	Value	Activity	Ec
43746	beta-galactosidase	-	3.2.1.23
43746	catalase	+	1.11.1.6
43746	cytochrome oxidase	+	1.9.3.1

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host	#Mammals	#Bovinae (Cow, Cattle)
#Host Body Product	#Fluids	#Milk

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
24225	raw cow’s milk	Southern Germany	Germany	DEU	Europe
43746	Raw cow's milk

Taxonmaps

69479

Global distribution of 16S sequence KP738715 (>99% sequence identity) for Pseudomonas from Microbeatlas

Aquatic Samples	830
Soil Samples	460
Animal Samples	1081
Plant Samples	571
Total Samples	2942

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
24225	1	Risk group (German classification)

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	MG221 assembly for Pseudomonas helleri DSM 29165	contig	GCA_001043025	1608996.3	2784132037	1608996	71.55

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
24225	Pseudomonas helleri strain DSM 29165 16S ribosomal RNA gene, partial sequence	KP738715	1525		1608996
124043	Pseudomonas helleri strain DSM 29165 16S ribosomal RNA gene, partial sequence.	OP164740	1436		1608996

GC content

@ref	GC-content (mol%)	Method
43746	58.1	genome sequence analysis
24225	58.1	sequence analysis

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: MG221 assembly for Pseudomonas helleri DSM 29165
NCBI: GCA_001043025

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	aerobe	80.94	no
125439	gram_stain	BacteriaNetⓘ	negative	97.94	no
125439	spore_formation	BacteriaNetⓘ	no	99.84	no
125439	motility	BacteriaNetⓘ	yes	80.50	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Pseudomonas sp. DSM 29165
PATRIC: 1608996.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	99.50	yes
125438	anaerobic	anaerobicⓘ	no	99.05	yes
125438	spore-forming	spore-formingⓘ	no	81.17	no
125438	aerobic	aerobicⓘ	yes	90.03	yes
125438	thermophilic	thermophileⓘ	no	97.50	yes
125438	flagellated	motile2+ⓘ	yes	90.18	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Pseudomonas helleri sp. nov. and Pseudomonas weihenstephanensis sp. nov., isolated from raw cow's milk.	von Neubeck M, Huptas C, Gluck C, Krewinkel M, Stoeckel M, Stressler T, Fischer L, Hinrichs J, Scherer S, Wenning M	Int J Syst Evol Microbiol	10.1099/ijsem.0.000852	2015

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#24225	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 29165
#43746	M. von Neubeck, C. Huptas, C. Glück, M. Krewinkel, M. Stoeckel, T. Stressler, L. Fischer, J. Hinrichs, S. Scherer, M. Wenning: Pseudomonas helleri sp. nov. and Pseudomonas weihenstephanensis sp. nov., isolated from raw cow's milk. IJSEM 66: 1163 - 1173 2016 ( DOI 10.1099/ijsem.0.000852 , PubMed 26675012 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Pseudomonas helleri (DSM 29165, LMG 28433, WS 4917)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Multimedia

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Observation

Metabolite utilization

Metabolite production

Enzymes

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_001043025

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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