Name: Testudinibacter aquarius ELNT2x
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 132402

Type strain:

Species: Testudinibacter aquarius

Strain Designation: ELNT2x

Culture col. no.: DSM 28140, CCUG 65146

Strain history: <- M.J. Hansen, Fac. of Health and Med. Scs., Univ. Copenhagen, Frederiksberg, Denmark; ELNT2x

NCBI tax ID(s): 1524974 (species)

Section

Testudinibacter aquarius ELNT2x is a microaerophile, mesophilic, Gram-negative bacterium that forms circular colonies and was isolated from oral cavity of a healthy captive Eastern long-necked turtle Chelodina longicollis.

colony-forming
Gram-negative
rod-shaped
mesophilic
microaerophile
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Pasteurellales*
	Family	*Pasteurellaceae*
	Genus	*Testudinibacter*
	Species	**Testudinibacter aquarius**
	Full Scientific Name (LPSN)	Testudinibacter aquarius Hansen et al. 2016

Information on morphological and physiological properties Morphology

[Ref.: #43824]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %99.983

[Ref.: #43824]	Cell shape	rod-shaped

[Ref.: #43824]	Motility	no

[Ref.: #43824]	Type of hemolysis	beta
[Ref.: #43824]	Hemolysis ability	1
[Ref.: #43824]	Colony size	0.5-1 mm
[Ref.: #43824]	Colony color	Grey
[Ref.: #43824]	Colony shape	circular
[Ref.: #43824]	Incubation period	1 day
[Ref.: #43824]	Cultivation medium used	BA

[Ref.: #63204]	Incubation period	1 day

Information on culture and growth conditions Culture and growth conditions

[Ref.: #43824]

Culture medium

[Ref.: #43824]

Culture medium growth

[Ref.: #24191]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #24191]

Culture medium growth

[Ref.: #24191]

Culture medium link

Medium recipe at MediaDive

[Ref.: #24191]

Culture medium composition

expand / minimize

[Ref.: #24191]

Culture medium

BHI MEDIUM (DSMZ Medium 215)

[Ref.: #24191]

Culture medium growth

[Ref.: #24191]

Culture medium link

Medium recipe at MediaDive

[Ref.: #24191]

Culture medium composition

expand / minimize

[Ref.: #43824]

Culture medium

MacConkey agar

[Ref.: #43824]

Culture medium growth

	Temperatures	Kind of temperature		Temperature
[Ref.: #24191]			growth	37 °C
[Ref.: #63204]			growth	37 °C

[Ref.: #24191]	Temperature range	mesophilic
[Ref.: #63204]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #63204]

Oxygen tolerance

microaerophile

[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.999

[Ref.: #43824]	Observation	No requirement for X- or V-factors
[Ref.: #43824]	Observation	Growth occurs in the presence of 10 % carbon dioxide, under anaerobic conditions.

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68369]	adipate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68381]	alpha-cyclodextrin ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #43824]	amygdalin ChEBI	+	builds acid from
[Ref.: #68369]	arginine ChEBI	-	hydrolysis	* from API 20NE, API rID32STR
[Ref.: #68381]	D-arabitol ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #43824]	D-fructose ChEBI	+	builds acid from
[Ref.: #43824]	D-galactose ChEBI	+	builds acid from
[Ref.: #43824]	D-glucose ChEBI	+	builds acid from
[Ref.: #68369]	D-glucose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	+	fermentation	* from API 20NE
[Ref.: #43824]	D-mannitol ChEBI	+	builds acid from
[Ref.: #68369]	D-mannitol ChEBI	-	assimilation	* from API 20NE
[Ref.: #68381]	D-mannitol ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #43824]	D-mannose ChEBI	+	builds acid from
[Ref.: #68369]	D-mannose ChEBI	+	assimilation	* from API 20NE
[Ref.: #43824]	D-ribose ChEBI	+	builds acid from
[Ref.: #68381]	D-ribose ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #43824]	D-sorbitol ChEBI	+	builds acid from
[Ref.: #68381]	D-tagatose ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #43824]	D-xylose ChEBI	+	builds acid from
[Ref.: #68369]	decanoate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	esculin ChEBI	+	hydrolysis	* from API 20NE
[Ref.: #43824]	galactitol ChEBI	+	builds acid from
[Ref.: #43824]	gelatin ChEBI	-	hydrolysis
[Ref.: #68369]	gelatin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gluconate ChEBI	+	assimilation	* from API 20NE
[Ref.: #43824]	glucose ChEBI	-	fermentation
[Ref.: #68381]	glycogen ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	hippurate ChEBI	-	hydrolysis	* from API rID32STR
[Ref.: #43824]	inulin ChEBI	+	builds acid from
[Ref.: #43824]	L-arabinose ChEBI	+	builds acid from
[Ref.: #68369]	L-arabinose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68381]	L-arabinose ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #43824]	L-fucose ChEBI	+	builds acid from
[Ref.: #43824]	L-rhamnose ChEBI	+	builds acid from
[Ref.: #43824]	lactose ChEBI	+	builds acid from
[Ref.: #68381]	lactose ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68369]	malate ChEBI	+	assimilation	* from API 20NE
[Ref.: #43824]	maltose ChEBI	+	builds acid from
[Ref.: #68381]	maltose ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #68381]	melezitose ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #43824]	melibiose ChEBI	+	builds acid from
[Ref.: #68381]	melibiose ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #68381]	methyl beta-D-glucopyranoside ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #43824]	nitrate ChEBI	+	reduction
[Ref.: #68369]	nitrate ChEBI	+	reduction	* from API 20NE
[Ref.: #43824]	nitrite ChEBI	-	reduction
[Ref.: #68381]	pullulan ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #43824]	raffinose ChEBI	+	builds acid from
[Ref.: #68381]	raffinose ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #43824]	ribitol ChEBI	+	builds acid from
[Ref.: #43824]	salicin ChEBI	+	builds acid from
[Ref.: #68381]	sorbitol ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #43824]	sucrose ChEBI	+	builds acid from
[Ref.: #68381]	sucrose ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #43824]	trehalose ChEBI	+	builds acid from
[Ref.: #68381]	trehalose ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #68369]	tryptophan ChEBI	+	energy source	* from API 20NE
[Ref.: #68381]	urea ChEBI	+	hydrolysis	* from API rID32STR
[Ref.: #68369]	urea ChEBI	-	hydrolysis	* from API 20NE
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #43824]	acetoin ChEBI	yes
[Ref.: #43824]	indole ChEBI	yes
[Ref.: #68381]	acetoin ChEBI	no	* from API rID32STR
[Ref.: #68369]	indole ChEBI	yes	* from API 20NE

	Metabolite	Voges-Proskauer-test	Methylred-test	Indole test
[Ref.: #43824]	acetoin ChEBI	+
[Ref.: #43824]	glucose ChEBI		-
[Ref.: #68381]	acetoin ChEBI	-			* from API rID32STR
[Ref.: #68369]	indole ChEBI			+	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #68381]	Alanyl-Phenylalanyl-Proline arylamidase	-		* from API rID32STR
[Ref.: #43824]	alkaline phosphatase	-	3.1.3.1
[Ref.: #68381]	alkaline phosphatase	+	3.1.3.1	* from API rID32STR
[Ref.: #68381]	alpha-galactosidase	+	3.2.1.22	* from API rID32STR
[Ref.: #43824]	alpha-glucosidase	-	3.2.1.20
[Ref.: #43824]	arginine dihydrolase	-	3.5.3.6
[Ref.: #68381]	arginine dihydrolase	-	3.5.3.6	* from API rID32STR, API 20NE
[Ref.: #68381]	beta-galactosidase	+	3.2.1.23	* from API rID32STR
[Ref.: #68369]	beta-glucosidase	+	3.2.1.21	* from API 20NE
[Ref.: #68381]	beta-glucosidase	-	3.2.1.21	* from API rID32STR
[Ref.: #68381]	beta-glucuronidase	-	3.2.1.31	* from API rID32STR
[Ref.: #68381]	beta-mannosidase	-	3.2.1.25	* from API rID32STR
[Ref.: #43824]	catalase	+	1.11.1.6
[Ref.: #43824]	cytochrome oxidase	+	1.9.3.1
[Ref.: #68369]	gelatinase	-		* from API 20NE
[Ref.: #68381]	glycyl tryptophan arylamidase	-		* from API rID32STR
[Ref.: #43824]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68381]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API rID32STR
[Ref.: #43824]	ornithine decarboxylase	+	4.1.1.17
[Ref.: #68381]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API rID32STR
[Ref.: #43824]	urease	-	3.5.1.5
[Ref.: #68381]	urease	+	3.5.1.5	* from API rID32STR
[Ref.: #68369]	urease	-	3.5.1.5	* from API 20NE
	Only first 10 entries are displayed. Click here to see all.

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #24191]	24175	+	+	+	-	-	+	-	+	+	-	+	-	+/-	+/-	+	-	-	+	-	-	not determinedn.d.

API® rID32STR:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Arginine dihydrolaseADH (Arg)	beta-Glucosidasebeta GLU	beta-Galactosidasebeta GAR	beta-Glucuronidasebeta GUR	alpha-Galactosidasealpha GAL	Alkaline PhosphatasePAL	Acid from D-riboseRIB	Acid from D-mannitolMAN	Acid from sorbitolSOR	Acid from lactoseLAC	Acid from trehaloseTRE	Acid from raffinoseRAF	Acid from sucroseSAC	Acid from L-arabinoseLARA	Acid from D-arabitolDARL	Acid from alpha-cyclodextrinCDEX	Acetoin production (Voges Proskauer test)VP	Alanyl-Phenylalanyl-Proline arylamidaseAPPA	beta-Galactosidasebeta GAL	Pyrrolidonyl arylamidasePyrA	N-Acetyl-glucosaminidasebeta NAG	Glycyl-tryptophan arylamidaseGTA	Hydrolysis of hippuric acidHIP	Acid from glycogenGLYG	Acid from pullulanPUL	Acid from maltoseMAL	Acid from melibioseMEL	Acid from melezitoseMLZ	Acidification of methyl beta-D-glucopyranosideMbeta DG	Acid from D-tagatoseTAG	beta-Mannosidasebeta MAN	Urease/urea hydrolysisURE
[Ref.: #24191]	10633	-	-	+	-	+	+	+	-	-	-	+	+	+	-	-	-	-	-	+	-	-	-	-	-	-	+	+	-	+	-	-	+

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #24191]	Sample type/isolated from	oral cavity of a healthy captive Eastern long-necked turtle Chelodina longicollis
[Ref.: #24191]	Host species	Chelodina longicollis
[Ref.: #24191]	Geographic location (country and/or sea, region)	Randers, Randers Regnskov
[Ref.: #24191]	Country	Denmark
[Ref.: #24191]	Country ISO 3 Code	DNK
[Ref.: #24191]	Continent	Europe

[Ref.: #43824]	Sample type/isolated from	The oral cavity of a captive eatern long-necked turtle
[Ref.: #43824]	Geographic location (country and/or sea, region)	Randers Regnskov
[Ref.: #43824]	Country	Denmark
[Ref.: #43824]	Country ISO 3 Code	DNK
[Ref.: #43824]	Continent	Europe
[Ref.: #43824]	Isolation date	2012

[Ref.: #63204]	Sample type/isolated from	Oral cavity of Eastern long-necked turtle
[Ref.: #63204]	Sampling date	2012-03-01
[Ref.: #63204]	Geographic location (country and/or sea, region)	Randers Regnskov
[Ref.: #63204]	Country	Denmark
[Ref.: #63204]	Country ISO 3 Code	DNK
[Ref.: #63204]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Host	#Reptilia	-
#Host Body-Site	#Oral cavity and airways	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence KJ632971 (>99% sequence identity) for Testudinibacter aquarius subclade from Microbeatlas

Aquatic Samples	207
Soil Samples	8
Animal Samples	481
Plant Samples	18
Total Samples	714

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #24191]	Testudinibacter aquarius strain ELNT2x 16S ribosomal RNA gene, partial sequence	KJ632971	1412	ENA	1524974 tax ID

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Testudinibacter aquarius DSM 28140	GCA_004342725	scaffold	GenBank	1524974 tax ID	*
[Ref.: #66792]	Testudinibacter aquarius ELNT2X	GCA_009761355	contig	GenBank	1524974 tax ID	*
[Ref.: #66792]	Testudinibacter aquarius ELNT2x	GCA_006227605	contig	GenBank	1524974 tax ID	*
[Ref.: #66792]	Pseudomonas sp. DSM 28140	1608993.3	wgs	PATRIC	1524974 tax ID	*
[Ref.: #66792]	Testudinibacter aquarius strain DSM 28140	1524974.3	wgs	PATRIC	1524974 tax ID	*
[Ref.: #66792]	Testudinibacter aquarius DSM 28140	2788499827	draft	JGI IMG/M	1524974 tax ID	*

[Ref.: #24191]

GC-content

42.8 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Testudinibacter aquarius DSM 28140 NCBI: GCA_004342725.1
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Testudinibacter aquarius DSM 28140 NCBI: GCA_004342725.1
[Ref.: #69480]	motile	no	88.461	yes
[Ref.: #69480]	flagellated	no	93.609	yes
[Ref.: #69480]	gram-positive	no	98.584	yes
[Ref.: #69480]	anaerobic	no	93.51	yes
[Ref.: #69480]	aerobic	no	80.72	yes
[Ref.: #69480]	halophile	no	64.883	no
[Ref.: #69480]	spore-forming	no	98.725	no
[Ref.: #69480]	glucose-util	yes	69.393	yes
[Ref.: #69480]	thermophile	no	98.75	yes
[Ref.: #69480]	glucose-ferment	yes	59.956	no

Availability in culture collections External links

[Ref.: #24191]

Culture collection no.

DSM 28140, CCUG 65146

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Testudinibacter aquarius gen. nov., sp. nov., a member of the family Pasteurellaceae isolated from the oral cavity of freshwater turtles.	Hansen MJ, Pennanen EAE, Bojesen AM, Christensen H, Bertelsen MF	Int J Syst Evol Microbiol	10.1099/ijsem.0.000759	2015	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#24191

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 28140

#43824

Mie Johanne Hansen, Elin Anna Erica Pennanen, Anders Miki Bojesen, Henrik Christensen, Mads Frost Bertelsen: Testudinibacter aquarius gen. nov., sp. nov., a member of the family Pasteurellaceae isolated from the oral cavity of freshwater turtles. IJSEM 66: 567 - 573 2016 ( DOI 10.1099/ijsem.0.000759 , PubMed 26554864 )

#63204 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 65146

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68369

Automatically annotated from API 20NE .

#68381

Automatically annotated from API rID32STR .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

* These data were automatically processed and therefore are not curated

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Testudinibacter aquarius DSM 28140

Testudinibacter aquarius DSM 28140

Change proposal

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