Testudinibacter aquarius (DSM 28140, CCUG 65146, ELNT2x)

Name: Testudinibacter aquarius (DSM 28140, CCUG 65146, ELNT2x)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 132402

Type strain

Strain Designation ELNT2x

Culture col. no. DSM 28140 CCUG 65146 ELNT2x

NCBI tax ID(s) 1524974

Special Collections DSMZ CCUG Literature strains

History <- M.J. Hansen, Fac. of Health and Med. Scs., Univ. Copenhagen, Frederiksberg, Denmark; ELNT2x

Links

Testudinibacter aquarius ELNT2x is a microaerophile, Gram-negative, rod-shaped bacterium that forms circular colonies and was isolated from oral cavity of a healthy captive Eastern long-necked turtle Chelodina longicollis.

Gram-negative rod-shaped colony-forming microaerophile genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 396474

DSM 29140,LMG 28438,DSM 28140,CCUG 65146T

@ref 20215

Last LPSN update 2026-02-09 11:31:42

Domain Bacteria

Phylum Pseudomonadota

Class Gammaproteobacteria

Order Pasteurellales

Family Pasteurellaceae

Genus Testudinibacter

Species Testudinibacter aquarius

Full scientific name Testudinibacter aquarius Hansen et al. 2016

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility	Confidence
43824	negative	rod-shaped
125438	negative			97.854

Colony morphology

@ref	Type of hemolysis	Hemolysis ability	Colony size	Colony color	Colony shape	Incubation period	Medium used
43824	beta	1	0.5-1 mm	Grey	circular	1 day	BA
63204						1 day

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
43824	BA
24191	COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)	Medium recipe at MediaDive	Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
24191	BHI MEDIUM (DSMZ Medium 215)	Medium recipe at MediaDive	Name: BHI MEDIUM (DSMZ Medium 215) Composition: Brain heart infusion 37.0 g/l Distilled water
43824	MacConkey agar

Culture temp

@ref	Growth	Type	Temperature (°C)
24191	positive	growth	37
63204	positive	growth	37

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
63204	microaerophile
125439	obligate aerobe	94.5

Spore formation

@ref	Spore formation	Confidence
125439		92.6

Observation

@ref	Observation
43824	No requirement for X- or V-factors
43824	Growth occurs in the presence of 10 % carbon dioxide, under anaerobic conditions.

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68369	17128 ChEBI	adipate	-	assimilation	from API 20NE
68381	40585 ChEBI	alpha-cyclodextrin	-	builds acid from	from API rID32STR
43824	27613 ChEBI	amygdalin	+	builds acid from
68381	29016 ChEBI	arginine	-	hydrolysis	from API rID32STR
68369	29016 ChEBI	arginine	-	hydrolysis	from API 20NE
68381	18333 ChEBI	D-arabitol	-	builds acid from	from API rID32STR
43824	15824 ChEBI	D-fructose	+	builds acid from
43824	12936 ChEBI	D-galactose	+	builds acid from
43824	17634 ChEBI	D-glucose	+	builds acid from
68369	17634 ChEBI	D-glucose	+	assimilation	from API 20NE
68369	17634 ChEBI	D-glucose	+	fermentation	from API 20NE
43824	16899 ChEBI	D-mannitol	+	builds acid from
68381	16899 ChEBI	D-mannitol	-	builds acid from	from API rID32STR
68369	16899 ChEBI	D-mannitol	-	assimilation	from API 20NE
43824	16024 ChEBI	D-mannose	+	builds acid from
68369	16024 ChEBI	D-mannose	+	assimilation	from API 20NE
43824	16988 ChEBI	D-ribose	+	builds acid from
68381	16988 ChEBI	D-ribose	+	builds acid from	from API rID32STR
43824	17924 ChEBI	D-sorbitol	+	builds acid from
68381	16443 ChEBI	D-tagatose	-	builds acid from	from API rID32STR
43824	65327 ChEBI	D-xylose	+	builds acid from
68369	27689 ChEBI	decanoate	-	assimilation	from API 20NE
68369	4853 ChEBI	esculin	+	hydrolysis	from API 20NE
43824	16813 ChEBI	galactitol	+	builds acid from
43824	5291 ChEBI	gelatin	-	hydrolysis
68369	5291 ChEBI	gelatin	-	hydrolysis	from API 20NE
68369	24265 ChEBI	gluconate	+	assimilation	from API 20NE
43824	17234 ChEBI	glucose	-	fermentation
68381	28087 ChEBI	glycogen	-	builds acid from	from API rID32STR
68381	606565 ChEBI	hippurate	-	hydrolysis	from API rID32STR
43824	15443 ChEBI	inulin	+	builds acid from
43824	30849 ChEBI	L-arabinose	+	builds acid from
68381	30849 ChEBI	L-arabinose	-	builds acid from	from API rID32STR
68369	30849 ChEBI	L-arabinose	-	assimilation	from API 20NE
43824	18287 ChEBI	L-fucose	+	builds acid from
43824	62345 ChEBI	L-rhamnose	+	builds acid from
43824	17716 ChEBI	lactose	+	builds acid from
68381	17716 ChEBI	lactose	-	builds acid from	from API rID32STR
68369	25115 ChEBI	malate	+	assimilation	from API 20NE
43824	17306 ChEBI	maltose	+	builds acid from
68381	17306 ChEBI	maltose	+	builds acid from	from API rID32STR
68381	6731 ChEBI	melezitose	-	builds acid from	from API rID32STR
43824	28053 ChEBI	melibiose	+	builds acid from
68381	28053 ChEBI	melibiose	+	builds acid from	from API rID32STR
68381	320055 ChEBI	methyl beta-D-glucopyranoside	+	builds acid from	from API rID32STR
43824	17632 ChEBI	nitrate	+	reduction
68369	17632 ChEBI	nitrate	+	reduction	from API 20NE
43824	16301 ChEBI	nitrite	-	reduction
68381	27941 ChEBI	pullulan	-	builds acid from	from API rID32STR
43824	16634 ChEBI	raffinose	+	builds acid from
68381	16634 ChEBI	raffinose	+	builds acid from	from API rID32STR
43824	15963 ChEBI	ribitol	+	builds acid from
43824	17814 ChEBI	salicin	+	builds acid from
68381	30911 ChEBI	sorbitol	-	builds acid from	from API rID32STR
43824	17992 ChEBI	sucrose	+	builds acid from
68381	17992 ChEBI	sucrose	+	builds acid from	from API rID32STR
43824	27082 ChEBI	trehalose	+	builds acid from
68381	27082 ChEBI	trehalose	+	builds acid from	from API rID32STR
68369	27897 ChEBI	tryptophan	+	energy source	from API 20NE
68381	16199 ChEBI	urea	+	hydrolysis	from API rID32STR
68369	16199 ChEBI	urea	-	hydrolysis	from API 20NE

Metabolite production

@ref	Chebi-ID	Metabolite
43824	15688 ChEBI	acetoin
68381	15688 ChEBI	acetoin	from API rID32STR
43824	35581 ChEBI	indole
68369	35581 ChEBI	indole	from API 20NE

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Methylred-test	Indole test
43824	15688 ChEBI	acetoin	+
68381	15688 ChEBI	acetoin	-			from API rID32STR
43824	17234 ChEBI	glucose		-
68369	35581 ChEBI	indole			+	from API 20NE

Enzymes

@ref	Value	Activity	Ec
68381	Alanyl-Phenylalanyl-Proline arylamidase	-		from API rID32STR
43824	alkaline phosphatase	-	3.1.3.1
68381	alkaline phosphatase	+	3.1.3.1	from API rID32STR
68381	alpha-galactosidase	+	3.2.1.22	from API rID32STR
43824	alpha-glucosidase	-	3.2.1.20
43824	arginine dihydrolase	-	3.5.3.6
68381	arginine dihydrolase	-	3.5.3.6	from API rID32STR
68369	arginine dihydrolase	-	3.5.3.6	from API 20NE
68381	beta-galactosidase	+	3.2.1.23	from API rID32STR
68381	beta-glucosidase	-	3.2.1.21	from API rID32STR
68369	beta-glucosidase	+	3.2.1.21	from API 20NE
68381	beta-glucuronidase	-	3.2.1.31	from API rID32STR
68381	beta-mannosidase	-	3.2.1.25	from API rID32STR
43824	catalase	+	1.11.1.6
43824	cytochrome oxidase	+	1.9.3.1
68369	gelatinase	-		from API 20NE
68381	glycyl tryptophan arylamidase	-		from API rID32STR
43824	lysine decarboxylase	-	4.1.1.18
68381	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API rID32STR
43824	ornithine decarboxylase	+	4.1.1.17
68381	pyrrolidonyl arylamidase	-	3.4.19.3	from API rID32STR
43824	urease	-	3.5.1.5
68381	urease	+	3.5.1.5	from API rID32STR
68369	urease	-	3.5.1.5	from API 20NE

API 20NE

@ref	Reduction of nitratesNO3	TRP	GLU_ Ferm	ADH (Arg)	URE	ESC	GEL	PNPG	GLU_ Assim	ARA	MNE	MAN	NAG	MAL	GNT	CAP	ADI	MLT	CIT	PAC	OX
24191	+	+	+	-	-	+	-	+	+	-	+	-	+/-	+/-	+	-	-	+	-	-	not determinedn.d.

API rID32STR

@ref	ADH (Arg)	beta GLU	beta GAR	beta GUR	alpha GAL	PAL	RIB	MAN	SOR	LAC	TRE	RAF	SAC	LARA	DARL	Acid from alpha-cyclodextrinCDEX	Acetoin production (Voges Proskauer test)VP	Alanyl-Phenylalanyl-Proline arylamidaseAPPA	beta GAL	Pyrrolidonyl arylamidasePyrA	N-Acetyl-glucosaminidasebeta NAG	Glycyl-tryptophan arylamidaseGTA	HIP	GLYG	PUL	MAL	MEL	MLZ	Acidification of methyl beta-D-glucopyranosideMbeta DG	TAG	beta MAN	URE
24191	-	-	+	-	+	+	+	-	-	-	+	+	+	-	-	-	-	-	+	-	-	-	-	-	-	+	+	-	+	-	-	+

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2
#Host	#Reptilia
#Host Body-Site	#Oral cavity and airways

Isolation

@ref	Sample type	Host species	Geographic location	Country	Country ISO 3 Code	Continent	Isolation date	Sampling date
24191	oral cavity of a healthy captive Eastern long-necked turtle Chelodina longicollis	Chelodina longicollis	Randers, Randers Regnskov	Denmark	DNK	Europe
43824	The oral cavity of a captive eatern long-necked turtle		Randers Regnskov	Denmark	DNK	Europe	2012
63204	Oral cavity of Eastern long-necked turtle		Randers Regnskov	Denmark	DNK	Europe		2012-03-01

Taxonmaps

69479

Global distribution of 16S sequence KJ632971 (>99% sequence identity) for Testudinibacter aquarius subclade from Microbeatlas

Aquatic Samples	207
Soil Samples	8
Animal Samples	481
Plant Samples	18
Total Samples	714

Interaction and safety

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM434272v1 assembly for Testudinibacter aquarius DSM 28140	scaffold	GCA_004342725	1524974.3	2788499827	1524974	74.47
66792	ASM976135v1 assembly for Testudinibacter aquarius ELNT2X	contig	GCA_009761355	1524974.5		1524974	62.6
66792	ASM622760v1 assembly for Testudinibacter aquarius ELNT2x	contig	GCA_006227605	1524974.4	8117595250	1524974	32.89

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
24191	Testudinibacter aquarius strain ELNT2x 16S ribosomal RNA gene, partial sequence	KJ632971	1412		1524974

GC content

24191

GC-content (mol%)42.8

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Testudinibacter aquarius DSM 28140
NCBI: GCA_004342725

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	92.60	no
125439	motility	BacteriaNetⓘ	yes	55.50	no
125439	gram_stain	BacteriaNetⓘ	positive	63.10	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	94.50	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Testudinibacter aquarius DSM 28140
NCBI: GCA_004342725.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	97.85	yes
125438	anaerobic	anaerobicⓘ	no	91.64	yes
125438	aerobic	aerobicⓘ	no	72.70	yes
125438	spore-forming	spore-formingⓘ	no	89.90	no
125438	thermophilic	thermophileⓘ	no	97.50	no
125438	flagellated	motile2+ⓘ	no	88.92	yes

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Testudinibacter aquarius gen. nov., sp. nov., a member of the family Pasteurellaceae isolated from the oral cavity of freshwater turtles.	Hansen MJ, Pennanen EAE, Bojesen AM, Christensen H, Bertelsen MF	Int J Syst Evol Microbiol	10.1099/ijsem.0.000759	2015

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#24191	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 28140
#43824	Mie Johanne Hansen, Elin Anna Erica Pennanen, Anders Miki Bojesen, Henrik Christensen, Mads Frost Bertelsen: Testudinibacter aquarius gen. nov., sp. nov., a member of the family Pasteurellaceae isolated from the oral cavity of freshwater turtles. IJSEM 66: 567 - 573 2016 ( DOI 10.1099/ijsem.0.000759 , PubMed 26554864 )
#63204	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 65146
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#68369	Automatically annotated from API 20NE .
#68381	Automatically annotated from API rID32STR .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Testudinibacter aquarius (DSM 28140, CCUG 65146, ELNT2x)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Observation

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API 20NE

API rID32STR

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Sequence information

Genome sequences

Genome browser: GCA_004342725

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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