Name: Kroppenstedtia pulmonis 165-08
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 132400

Type strain:

Species: Kroppenstedtia pulmonis

Strain Designation: 165-08, W9323

Culture col. no.: DSM 45752, CCUG 68107

Strain history: <- M. Bell, CDC; W9323 <- New York State Health Department

NCBI tax ID(s): 1380685 (species)

SI-ID 400135

DSM 45752, CCUG 68107

Section

Kroppenstedtia pulmonis 165-08 is an aerobe, spore-forming, thermophilic human pathogen that was isolated from lung biopsy of a 78-year-old male patient.

Gram-positive
spore-forming
aerobe
human pathogen
thermophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Thermoactinomycetaceae*
	Genus	*Kroppenstedtia*
	Species	**Kroppenstedtia pulmonis**
	Full Scientific Name (LPSN)	Kroppenstedtia pulmonis Bell et al. 2016

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #63456]

Incubation period

6-7 days

Information on culture and growth conditions Culture and growth conditions

[Ref.: #24189]

Culture medium

BHI MEDIUM (DSMZ Medium 215)

[Ref.: #24189]

Culture medium growth

[Ref.: #24189]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #24189]

Culture medium

TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)

[Ref.: #24189]

Culture medium growth

[Ref.: #24189]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #24189]

Culture medium

TRYPTICASE SOY BROTH AGAR+BLOOD (DSMZ Medium 535a)

[Ref.: #24189]

Culture medium growth

[Ref.: #24189]

Culture medium link

Medium recipe at MediaDive

[Ref.: #24189]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #24189]			growth	45 °C
[Ref.: #63456]			growth	42 °C

[Ref.: #24189]	Temperature range	thermophilic
[Ref.: #63456]	Temperature range	thermophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #63456]

Oxygen tolerance

aerobe

[Ref.: #69481]	Ability of spore formation	yes Confidence in %100
[Ref.: #69480]	Ability of spore formation	yes Confidence in %100

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #24189]	Sample type/isolated from	lung biopsy of a 78-year-old male patient
[Ref.: #24189]	Geographic location (country and/or sea, region)	New York
[Ref.: #24189]	Country	USA
[Ref.: #24189]	Country ISO 3 Code	USA
[Ref.: #24189]	Continent	North America

[Ref.: #63456]	Sample type/isolated from	Human lung sample
[Ref.: #63456]	Sampling date	2008
[Ref.: #63456]	Geographic location (country and/or sea, region)	New York
[Ref.: #63456]	Country	USA
[Ref.: #63456]	Country ISO 3 Code	USA
[Ref.: #63456]	Continent	North America
* marker position based on {}

Isolation sources categories

#Infection	#Patient	#Biopsy
#Infection	#Patient	-
#Host Body-Site	#Oral cavity and airways	#Lung
#Host	#Human	#Male

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence KC875234 (>99% sequence identity) for Kroppenstedtia pulmonis subclade from Microbeatlas

Aquatic Samples	142
Soil Samples	989
Animal Samples	432
Plant Samples	197
Total Samples	1760

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #24189]	Pathogenicity (human)	yes, in single cases
[Ref.: #24189]	Biosafety level	1	Risk group (German classification) According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #24189]	Kroppenstedtia pulmonis strain W9323 16S ribosomal RNA gene, partial sequence	KC875234	1473	ENA	1380685 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Kroppenstedtia pulmonis W9323	GCA_013265585	complete	GenBank	1380685 tax ID	*
[Ref.: #66792]	Kroppenstedtia pulmonis W9323	2910262097	complete	JGI IMG/M	1380685 tax ID	*

[Ref.: #24189]

GC-content

45.9 mol%

high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Kroppenstedtia pulmonis W9323 NCBI: GCA_013265585.1
[Ref.: #69481]	spore-forming	yes	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Kroppenstedtia pulmonis W9323 NCBI: GCA_013265585.1
[Ref.: #69480]	motile	no	80.768	no
[Ref.: #69480]	flagellated	no	91.437	no
[Ref.: #69480]	gram-positive	yes	88.416	no
[Ref.: #69480]	anaerobic	no	98.239	yes
[Ref.: #69480]	aerobic	yes	93.027	no
[Ref.: #69480]	halophile	no	67.133	no
[Ref.: #69480]	spore-forming	yes	94.31	no
[Ref.: #69480]	glucose-util	yes	89.57	no
[Ref.: #69480]	thermophile	yes	96.848	yes
[Ref.: #69480]	glucose-ferment	no	93.213	no

Availability in culture collections External links

[Ref.: #24189]

Culture collection no.

DSM 45752, CCUG 68107

[Ref.: #90742]

SI-ID 400135

Literature:

	Title	Authors	Journal	DOI	Year
Phylogeny	Kroppenstedtia pulmonis sp. nov. and Kroppenstedtia sanguinis sp. nov., isolated from human patients.	Bell ME, Lasker BA, Klenk HP, Hoyles L, Sproer C, Schumann P, Brown JM	Antonie Van Leeuwenhoek	10.1007/s10482-016-0663-z	2016	*
	Complete and Circularized Genome Assemblies of the Kroppenstedtia eburnea Genus Type Strain and the Kroppenstedtia pulmonis Species Type Strain with MiSeq and MinION Sequence Data.	Gulvik CA, Batra D, Rowe LA, Sheth M, Humrighouse BW, Howard DT, Lee J, McQuiston JR, Lasker BA	Microbiol Resour Announc	10.1128/MRA.00650-20	2020	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#24189

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 45752

#63456 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 68107

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#90742

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID400135.1 )

* These data were automatically processed and therefore are not curated

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Kroppenstedtia pulmonis W9323

Kroppenstedtia pulmonis W9323

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