Amycolatopsis sacchari A1714, K24, K24T | Type strain | DSM 44468, JCM 11272, CIP 107029, KCTC 9863, NBRC 100339, NCIMB 13786 | BacDiveID:13235

Strain identifier

BacDive ID: 13235

Type strain:

Species: Amycolatopsis sacchari

Strain Designation: A1714, K24, K24T

Culture col. no.: DSM 44468, JCM 11272, CIP 107029, KCTC 9863, NBRC 100339, NCIMB 13786

Strain history: CIP <- 2001, M. Goodfellow, Newcastle Univ., Newcastle, UK: strain K24T <- J. Lacey: strain A1714

NCBI tax ID(s): 115433 (species)

SI-ID 50638

NCIMB 13786, DSM 44468, KCTC 9863, CIP 107029, JCM 11272, NBRC 100339

Special Collections

Other strains from Amycolatopsis sacchari

Section

Amycolatopsis sacchari A1714 is an obligate aerobe, mesophilic, Gram-positive bacterium that builds an aerial mycelium and was isolated from floor dust of a hemp factory.

Gram-positive
rod-shaped
mesophilic
obligate aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	09-12-2024 (DD-MM-YYYY)
	Domain	*Bacteria*
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Pseudonocardiales*
	Family	*Pseudonocardiaceae*
	Genus	*Amycolatopsis*
	Species	**Amycolatopsis sacchari**
	Full Scientific Name (LPSN)	Amycolatopsis sacchari Goodfellow et al. 2001

Information on morphological and physiological properties Morphology

[Ref.: #122329]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %91.666

[Ref.: #122329]	Cell shape	rod-shaped

[Ref.: #122329]	Motility	no

[Ref.: #19670]	Colony color	Pastel yellow
[Ref.: #19670]	Incubation period	10-14 days
[Ref.: #19670]	Cultivation medium used	ISP 2

[Ref.: #19670]	Colony color	Pastel yellow
[Ref.: #19670]	Incubation period	10-14 days
[Ref.: #19670]	Cultivation medium used	ISP 3

[Ref.: #19670]	Incubation period	10-14 days
[Ref.: #19670]	Cultivation medium used	ISP 4

[Ref.: #19670]	Incubation period	10-14 days
[Ref.: #19670]	Cultivation medium used	ISP 5

[Ref.: #19670]	Incubation period	10-14 days
[Ref.: #19670]	Cultivation medium used	ISP 6

[Ref.: #19670]	Incubation period	10-14 days
[Ref.: #19670]	Cultivation medium used	ISP 7

[Ref.: #19670]	Multicellular complex forming ability	yes
[Ref.: #19670]	Multicellular complex name	Aerial Mycelium
[Ref.: #19670]	Multicellular complex color	White
[Ref.: #19670]	Medium Name (multicellularity)	ISP 3

[Ref.: #19670]	Multicellular complex forming ability	no
[Ref.: #19670]	Medium Name (multicellularity)	ISP 4

[Ref.: #19670]	Multicellular complex forming ability	no
[Ref.: #19670]	Medium Name (multicellularity)	ISP 5

[Ref.: #19670]	Multicellular complex forming ability	no
[Ref.: #19670]	Medium Name (multicellularity)	ISP 6

[Ref.: #19670]	Multicellular complex forming ability	no
[Ref.: #19670]	Medium Name (multicellularity)	ISP 7

[Ref.: #19670]	Medium Name (multicellularity)	ISP 2

Multimedia content

Caption:

Medium 83 28°C

Intellectual property rights:

© Leibniz-Institut DSMZ

Information on culture and growth conditions Culture and growth conditions

Culture medium

CZAPEK PEPTONE AGAR (DSMZ Medium 83)

Culture medium growth

Culture medium link

Medium recipe at MediaDive

Culture medium composition

expand / minimize

Culture medium

GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)

Culture medium growth

Culture medium link

Medium recipe at MediaDive

Culture medium composition

expand / minimize

Culture medium

ISP 2

Culture medium growth

Culture medium composition

expand / minimize

Culture medium

ISP 3

Culture medium growth

Culture medium composition

expand / minimize

Culture medium

ISP 4

Culture medium growth

Culture medium composition

expand / minimize

Culture medium

ISP 5

Culture medium growth

Culture medium composition

expand / minimize

Culture medium

ISP 6

Culture medium growth

Culture medium composition

expand / minimize

Culture medium

ISP 7

Culture medium growth

Culture medium composition

expand / minimize

Culture medium

MEDIUM 57 - for Streptomyces, Nocardioides, Lentzea albidocapillata and Streptoverticillium reticulum

Culture medium growth

Culture medium composition

expand / minimize

[Ref.: #122329]

Culture medium

CIP Medium 57

[Ref.: #122329]

Culture medium growth

[Ref.: #122329]

Culture medium link

Medium recipe provided by DSMZ

	Temperatures	Kind of temperature		Temperature
[Ref.: #11794]			growth	28 °C
[Ref.: #19670]			optimum	28 °C
[Ref.: #39634]			growth	30 °C
[Ref.: #67770]			growth	28 °C
[Ref.: #122329]			growth	25-45 °C
[Ref.: #122329]		no	growth	10 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #122329]

Oxygen tolerance

obligate aerobe

[Ref.: #19670]	Ability of spore formation	no
[Ref.: #69481]	Ability of spore formation	yes Confidence in %99

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #122329]		NaCl		growth	0-8	%
[Ref.: #122329]		NaCl	no	growth	10	%

Observation

quinones: MK-9(H₄)

	Metabolite utilization	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68371]		amygdalin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #19670]		arabinose ChEBI	+
[Ref.: #68371]		arbutin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68368]		arginine ChEBI	+	hydrolysis	* from API 20E
[Ref.: #68371]		cellobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #19670]		cellulose ChEBI	-
[Ref.: #122329]		citrate ChEBI	-	carbon source
[Ref.: #68368]		citrate ChEBI	+	assimilation	* from API 20E
[Ref.: #68371]		D-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]		D-fructose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68379]		D-glucose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68371]		D-lyxose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68379]		D-mannitol ChEBI	-	fermentation	* from API Coryne
[Ref.: #68371]		D-mannose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]		D-ribose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68379]		D-ribose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68371]		D-sorbitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]		D-tagatose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]		D-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68379]		D-xylose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68371]		erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #122329]		esculin ChEBI	+	hydrolysis
[Ref.: #68371]		esculin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68379]		esculin ChEBI	+	hydrolysis	* from API Coryne
[Ref.: #19670]		fructose ChEBI	+
[Ref.: #68371]		galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68379]		gelatin ChEBI	+	hydrolysis	* from API Coryne, API 20E
[Ref.: #68371]		gentiobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]		gluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #19670]		glucose ChEBI	+
[Ref.: #68371]		glycerol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]		glycogen ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68379]		glycogen ChEBI	-	fermentation	* from API Coryne
[Ref.: #68371]		inulin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]		L-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]		L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]		L-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]		L-sorbose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]		L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]		lactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68379]		lactose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68368]		lysine ChEBI	+	degradation	* from API 20E
[Ref.: #68371]		maltose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68379]		maltose ChEBI	-	fermentation	* from API Coryne
[Ref.: #19670]		mannitol ChEBI	+
[Ref.: #68371]		melezitose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]		melibiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]		methyl alpha-D-glucopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]		methyl alpha-D-mannoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]		methyl beta-D-xylopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #19670]		myo-inositol ChEBI	+
[Ref.: #68371]		N-acetylglucosamine ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #122329]		nitrate ChEBI	-	reduction
[Ref.: #122329]		nitrate ChEBI	-	respiration
[Ref.: #68379]		nitrate ChEBI	-	reduction	* from API Coryne
[Ref.: #122329]		nitrite ChEBI	-	reduction
[Ref.: #68368]		ornithine ChEBI	+	degradation	* from API 20E
[Ref.: #68371]		Potassium 2-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]		Potassium 5-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #19670]		raffinose ChEBI	-
[Ref.: #68371]		raffinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #19670]		rhamnose ChEBI	+/-
[Ref.: #68371]		ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]		salicin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]		starch ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #19670]		sucrose ChEBI	+
[Ref.: #68371]		sucrose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68379]		sucrose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68371]		trehalose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68368]		tryptophan ChEBI	-	energy source	* from API 20E
[Ref.: #68371]		turanose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68379]		urea ChEBI	+	hydrolysis	* from API Coryne, API 20E
[Ref.: #19670]		xylose ChEBI	-
		Only first 10 entries are displayed. Click here to see all.

	Antibiotica	Metabolite	Antibiotic sensitivity	Antibiotic resistance
[Ref.: #122329]		0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)	yes	no

	Metabolite production	Metabolite	Production
[Ref.: #122329]		indole ChEBI	no
[Ref.: #68368]		acetoin ChEBI	yes	* from API 20E
[Ref.: #68368]		hydrogen sulfide ChEBI	no	* from API 20E
[Ref.: #68368]		indole ChEBI	no	* from API 20E

	Physiological tests	Metabolite	Voges-Proskauer-test	Methylred-test	Indole test
[Ref.: #122329]		acetoin ChEBI	-
[Ref.: #122329]		glucose ChEBI		-
[Ref.: #68368]		acetoin ChEBI	+			* from API 20E
[Ref.: #68368]		indole ChEBI			-	* from API 20E

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #68382]		acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #122329]		alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]		alkaline phosphatase	+	3.1.3.1	* from API zym, API Coryne
[Ref.: #68382]		alpha-chymotrypsin	+	3.4.21.1	* from API zym
[Ref.: #68382]		alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]		alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]		alpha-glucosidase	+	3.2.1.20	* from API zym, API Coryne
[Ref.: #68382]		alpha-mannosidase	+	3.2.1.24	* from API zym
[Ref.: #122329]		amylase	-
[Ref.: #68368]		arginine dihydrolase	+	3.5.3.6	* from API 20E
[Ref.: #68382]		beta-galactosidase	+	3.2.1.23	* from API zym, API 20E
[Ref.: #68379]		beta-galactosidase	-	3.2.1.23	* from API Coryne
[Ref.: #68382]		beta-glucosidase	+	3.2.1.21	* from API zym, API Coryne
[Ref.: #68382]		beta-glucuronidase	-	3.2.1.31	* from API zym, API Coryne
[Ref.: #122329]		caseinase	+	3.4.21.50
[Ref.: #122329]		catalase	+	1.11.1.6
[Ref.: #68382]		cystine arylamidase	+	3.4.11.3	* from API zym
[Ref.: #122329]		DNase	-
[Ref.: #68382]		esterase lipase (C 8)	+		* from API zym
[Ref.: #122329]		gamma-glutamyltransferase	+	2.3.2.2
[Ref.: #122329]		gelatinase	+
[Ref.: #68379]		gelatinase	+		* from API Coryne, API 20E
[Ref.: #122329]		lecithinase	-
[Ref.: #68382]		leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #122329]		lipase	+
[Ref.: #122329]		lysine decarboxylase	-	4.1.1.18
[Ref.: #68368]		lysine decarboxylase	+	4.1.1.18	* from API 20E
[Ref.: #68382]		N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API zym, API Coryne
[Ref.: #68382]		naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #122329]		ornithine decarboxylase	-	4.1.1.17
[Ref.: #68368]		ornithine decarboxylase	+	4.1.1.17	* from API 20E
[Ref.: #122329]		oxidase	-
[Ref.: #122329]		protease	+
[Ref.: #68379]		pyrazinamidase	+	3.5.1.B15	* from API Coryne
[Ref.: #68379]		pyrrolidonyl arylamidase	-	3.4.19.3	* from API Coryne
[Ref.: #68382]		trypsin	+	3.4.21.4	* from API zym
[Ref.: #122329]		tryptophan deaminase	-
[Ref.: #68368]		tryptophan deaminase	-	4.1.99.1	* from API 20E
[Ref.: #122329]		tween esterase	+
[Ref.: #122329]		urease	+	3.5.1.5
[Ref.: #68368]		urease	+	3.5.1.5	* from API 20E, API Coryne
[Ref.: #68382]		valine arylamidase	+		* from API zym
		Only first 10 entries are displayed. Click here to see all.

API® 20E:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	beta-GalactosidaseONPG	Arginine dihydrolaseADH (Arg)	Lysine decarboxylaseLDC (Lys)	Ornithine decarboxylaseODC	Citrate utilizationCIT	H2S productionH2S	Urease/urea hydrolysisURE	Tryptophan deaminaseTDA (Trp)	Indole productionIND	Acetoin production (Voges Proskauer test)VP	Gelatinase/gelatin hydrolysisGEL	Fermentation/oxidation of D-glucoseGLU	Fermentation/oxidation of D-mannitolMAN	Fermentation/oxidation of inositolINO	Fermentation/oxidation of sorbitolSor	Fermentation/oxidation of L-rhamnoseRHA	Fermentation/oxidation of sucroseSAC	Fermentation/oxidation of melibioseMEL	Fermentation/oxidation of amygdalinAMY	Fermentation/oxidation of L-arabinoseARA	Cytochrome oxidaseOX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	Oxidative catabolisation of D-glucoseOF-O	Fermentation of D-glucoseOF-F
[Ref.: #19670]	493	+	+	+	+	+	-	+	-	-	+	+	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #122329]	10808	not determinedn.d.	-	-	-	-	-	-	-	-	-	+/-	+/-	-	-	-	+/-	-	+/-	+/-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	+/-	-	-	-	-	+/-	-	+/-	-	-	-	-

API® Biotype100:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
[Ref.: #122329]	1966	not determinedn.d.	+	+	+	+	+	-	-	+	-	-	-	-	+	-	-	+	+	-	+	+	+	+	-	+	-	-	+	-	+	-	-	+	+	+	-	-	+	-	-	-	-	-	-	+	-	-	-	+	-	+	+	-	-	+	+	-	+	-	-	+	+	+	+	+	+	-	+	-	-	+	-	-	-	-	-	+	-	-	-	+	+	-	+	-	-	-	+	-	+	-	+	+	-	+	+	-	+	+	-

API® Coryne:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitrateNIT	PyrazinamidasePYZ	Pyrrolidonyl arylamidasePYRA	Alkaline phosphatasePAL	beta-Glucuronidasebeta GUR	beta-Galactosidasebeta GAL	alpha-Glucosidasealpha GLU	N-Acetyl-beta-Glucosidasebeta NAG	Esculin hydrolysis/beta-GlucosidaseESC	Urease/urea hydrolysisURE	Gelatinase/gelatin hydrolysisGEL	Control fermentationControl	Fermentation of D-glucoseGLU	Fermentation of D-riboseRIB	Fermentation of D-xyloseXYL	Fermentation of D-mannitolMAN	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of sucroseSAC	Fermentation of glycogenGLYG	CatalaseCAT
[Ref.: #19670]	244	-	+	-	+	-	-	+	+	+	+	+	not determinedn.d.	-	-	-	-	-	-	-	-	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #19670]	495	not determinedn.d.	+	-	+	-	+	+	+	+	+	+	+	-	+	-	+	+	+	+	-
[Ref.: #122329]	10012	-	+	+	+	+	+	+	+	+	+	+	+	-	+	-	+	+	+	+	-

	Pathway	Enzyme coverage	Annotated reactions	External links
	Pathway annotation	Computationally determined by
[Ref.: #66794]	palmitate biosynthesis	100	22 of 22
[Ref.: #66794]	resorcinol degradation	100	2 of 2
[Ref.: #66794]	mannosylglycerate biosynthesis	100	2 of 2
[Ref.: #66794]	L-lactaldehyde degradation	100	3 of 3
[Ref.: #66794]	propanol degradation	100	7 of 7
[Ref.: #66794]	cardiolipin biosynthesis	100	7 of 7
[Ref.: #66794]	suberin monomers biosynthesis	100	2 of 2
[Ref.: #66794]	Entner Doudoroff pathway	100	10 of 10
[Ref.: #66794]	anapleurotic synthesis of oxalacetate	100	1 of 1
[Ref.: #66794]	adipate degradation	100	2 of 2
[Ref.: #66794]	gallate degradation	100	5 of 5
[Ref.: #66794]	biotin biosynthesis	100	4 of 4
[Ref.: #66794]	starch degradation	100	10 of 10
[Ref.: #66794]	CDP-diacylglycerol biosynthesis	100	2 of 2
[Ref.: #66794]	enterobactin biosynthesis	100	3 of 3
[Ref.: #66794]	ppGpp biosynthesis	100	4 of 4
[Ref.: #66794]	ceramide biosynthesis	100	1 of 1
[Ref.: #66794]	taurine degradation	100	1 of 1
[Ref.: #66794]	methylglyoxal degradation	100	5 of 5
[Ref.: #66794]	butanoate fermentation	100	4 of 4
[Ref.: #66794]	acetate fermentation	100	4 of 4
[Ref.: #66794]	valine metabolism	100	9 of 9
[Ref.: #66794]	4-hydroxymandelate degradation	100	9 of 9
[Ref.: #66794]	ethanol fermentation	100	2 of 2
[Ref.: #66794]	aerobactin biosynthesis	100	1 of 1
[Ref.: #66794]	phenylmercury acetate degradation	100	2 of 2
[Ref.: #66794]	cis-vaccenate biosynthesis	100	2 of 2
[Ref.: #66794]	glycogen metabolism	100	5 of 5
[Ref.: #66794]	coenzyme A metabolism	100	4 of 4
[Ref.: #66794]	sulfopterin metabolism	100	4 of 4
[Ref.: #66794]	formaldehyde oxidation	100	3 of 3
[Ref.: #66794]	reductive acetyl coenzyme A pathway	100	7 of 7
[Ref.: #66794]	vitamin K metabolism	100	5 of 5
[Ref.: #66794]	UDP-GlcNAc biosynthesis	100	3 of 3
[Ref.: #66794]	folate polyglutamylation	100	1 of 1
[Ref.: #66794]	kanosamine biosynthesis II	100	2 of 2
[Ref.: #66794]	phenylacetate degradation (aerobic)	100	5 of 5
[Ref.: #66794]	cellulose degradation	100	5 of 5
[Ref.: #66794]	flavin biosynthesis	93.33	14 of 15
[Ref.: #66794]	leucine metabolism	92.31	12 of 13
[Ref.: #66794]	phenylalanine metabolism	92.31	12 of 13
[Ref.: #66794]	pentose phosphate pathway	90.91	10 of 11
[Ref.: #66794]	propionate fermentation	90	9 of 10
[Ref.: #66794]	alanine metabolism	89.66	26 of 29
[Ref.: #66794]	tryptophan metabolism	89.47	34 of 38
[Ref.: #66794]	serine metabolism	88.89	8 of 9
[Ref.: #66794]	aspartate and asparagine metabolism	88.89	8 of 9
[Ref.: #66794]	pyrimidine metabolism	88.89	40 of 45
[Ref.: #66794]	CO2 fixation in Crenarchaeota	88.89	8 of 9
[Ref.: #66794]	allantoin degradation	88.89	8 of 9
[Ref.: #66794]	chorismate metabolism	88.89	8 of 9
[Ref.: #66794]	molybdenum cofactor biosynthesis	88.89	8 of 9
[Ref.: #66794]	ketogluconate metabolism	87.5	7 of 8
[Ref.: #66794]	peptidoglycan biosynthesis	86.67	13 of 15
[Ref.: #66794]	heme metabolism	85.71	12 of 14
[Ref.: #66794]	glutamate and glutamine metabolism	85.71	24 of 28
[Ref.: #66794]	ubiquinone biosynthesis	85.71	6 of 7
[Ref.: #66794]	citric acid cycle	85.71	12 of 14
[Ref.: #66794]	aclacinomycin biosynthesis	85.71	6 of 7
[Ref.: #66794]	vitamin B12 metabolism	85.29	29 of 34
[Ref.: #66794]	phenol degradation	85	17 of 20
[Ref.: #66794]	purine metabolism	84.04	79 of 94
[Ref.: #66794]	degradation of sugar acids	84	21 of 25
[Ref.: #66794]	lipid metabolism	83.87	26 of 31
[Ref.: #66794]	glycolate and glyoxylate degradation	83.33	5 of 6
[Ref.: #66794]	proline metabolism	81.82	9 of 11
[Ref.: #66794]	metabolism of disaccharids	81.82	9 of 11
[Ref.: #66794]	3-chlorocatechol degradation	80	4 of 5
[Ref.: #66794]	ethylmalonyl-CoA pathway	80	4 of 5
[Ref.: #66794]	threonine metabolism	80	8 of 10
[Ref.: #66794]	4-hydroxyphenylacetate degradation	80	8 of 10
[Ref.: #66794]	factor 420 biosynthesis	80	4 of 5
[Ref.: #66794]	hydrogen production	80	4 of 5
[Ref.: #66794]	tetrahydrofolate metabolism	78.57	11 of 14
[Ref.: #66794]	photosynthesis	78.57	11 of 14
[Ref.: #66794]	NAD metabolism	77.78	14 of 18
[Ref.: #66794]	degradation of hexoses	77.78	14 of 18
[Ref.: #66794]	urea cycle	76.92	10 of 13
[Ref.: #66794]	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	75	6 of 8
[Ref.: #66794]	cyclohexanol degradation	75	3 of 4
[Ref.: #66794]	glycogen biosynthesis	75	3 of 4
[Ref.: #66794]	degradation of aromatic, nitrogen containing compounds	75	9 of 12
[Ref.: #66794]	androgen and estrogen metabolism	75	12 of 16
[Ref.: #66794]	gluconeogenesis	75	6 of 8
[Ref.: #66794]	isoleucine metabolism	75	6 of 8
[Ref.: #66794]	3-phenylpropionate degradation	73.33	11 of 15
[Ref.: #66794]	vitamin B6 metabolism	72.73	8 of 11
[Ref.: #66794]	histidine metabolism	72.41	21 of 29
[Ref.: #66794]	tyrosine metabolism	71.43	10 of 14
[Ref.: #66794]	degradation of pentoses	71.43	20 of 28
[Ref.: #66794]	glutathione metabolism	71.43	10 of 14
[Ref.: #66794]	non-pathway related	71.05	27 of 38
[Ref.: #66794]	glycolysis	70.59	12 of 17
[Ref.: #66794]	oxidative phosphorylation	70.33	64 of 91
[Ref.: #66794]	myo-inositol biosynthesis	70	7 of 10
[Ref.: #66794]	methionine metabolism	69.23	18 of 26
[Ref.: #66794]	isoprenoid biosynthesis	69.23	18 of 26
[Ref.: #66794]	vitamin B1 metabolism	69.23	9 of 13
[Ref.: #66794]	degradation of sugar alcohols	68.75	11 of 16
[Ref.: #66794]	d-mannose degradation	66.67	6 of 9
[Ref.: #66794]	selenocysteine biosynthesis	66.67	4 of 6
[Ref.: #66794]	acetoin degradation	66.67	2 of 3
[Ref.: #66794]	cysteine metabolism	66.67	12 of 18
[Ref.: #66794]	(5R)-carbapenem carboxylate biosynthesis	66.67	2 of 3
[Ref.: #66794]	cyanate degradation	66.67	2 of 3
[Ref.: #66794]	octane oxidation	66.67	2 of 3
[Ref.: #66794]	d-xylose degradation	63.64	7 of 11
[Ref.: #66794]	cholesterol biosynthesis	63.64	7 of 11
[Ref.: #66794]	C4 and CAM-carbon fixation	62.5	5 of 8
[Ref.: #66794]	arginine metabolism	62.5	15 of 24
[Ref.: #66794]	dTDPLrhamnose biosynthesis	62.5	5 of 8
[Ref.: #66794]	phosphatidylethanolamine bioynthesis	61.54	8 of 13
[Ref.: #66794]	sulfate reduction	61.54	8 of 13
[Ref.: #66794]	polyamine pathway	60.87	14 of 23
[Ref.: #66794]	metabolism of amino sugars and derivatives	60	3 of 5
[Ref.: #66794]	creatinine degradation	60	3 of 5
[Ref.: #66794]	lysine metabolism	59.52	25 of 42
[Ref.: #66794]	carotenoid biosynthesis	59.09	13 of 22
[Ref.: #66794]	daunorubicin biosynthesis	55.56	5 of 9
[Ref.: #66794]	phenylpropanoid biosynthesis	53.85	7 of 13
[Ref.: #66794]	CMP-KDO biosynthesis	50	2 of 4
[Ref.: #66794]	toluene degradation	50	2 of 4
[Ref.: #66794]	ribulose monophosphate pathway	50	1 of 2
[Ref.: #66794]	glycine metabolism	50	5 of 10
[Ref.: #66794]	dolichol and dolichyl phosphate biosynthesis	50	1 of 2
[Ref.: #66794]	aminopropanol phosphate biosynthesis	50	1 of 2
[Ref.: #66794]	pantothenate biosynthesis	50	3 of 6
[Ref.: #66794]	lactate fermentation	50	2 of 4
[Ref.: #66794]	coenzyme M biosynthesis	50	5 of 10
[Ref.: #66794]	quinate degradation	50	1 of 2
[Ref.: #66794]	bile acid biosynthesis, neutral pathway	47.06	8 of 17
[Ref.: #66794]	arachidonic acid metabolism	44.44	8 of 18
[Ref.: #66794]	lipid A biosynthesis	44.44	4 of 9
[Ref.: #66794]	nitrate assimilation	44.44	4 of 9
[Ref.: #66794]	benzoyl-CoA degradation	42.86	3 of 7
[Ref.: #66794]	ascorbate metabolism	40.91	9 of 22
[Ref.: #66794]	glycine betaine biosynthesis	40	2 of 5
[Ref.: #66794]	D-cycloserine biosynthesis	40	2 of 5
[Ref.: #66794]	bacilysin biosynthesis	40	2 of 5
[Ref.: #66794]	lipoate biosynthesis	40	2 of 5
[Ref.: #66794]	arachidonate biosynthesis	40	2 of 5
[Ref.: #66794]	chlorophyll metabolism	38.89	7 of 18
[Ref.: #66794]	carnitine metabolism	37.5	3 of 8
[Ref.: #66794]	sphingosine metabolism	33.33	2 of 6
[Ref.: #66794]	acetyl CoA biosynthesis	33.33	1 of 3
[Ref.: #66794]	IAA biosynthesis	33.33	1 of 3
[Ref.: #66794]	mevalonate metabolism	28.57	2 of 7
[Ref.: #66794]	dolichyl-diphosphooligosaccharide biosynthesis	27.27	3 of 11
[Ref.: #66794]	methanogenesis from CO2	25	3 of 12
[Ref.: #66794]	catecholamine biosynthesis	25	1 of 4
		Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #11794]	Sample type/isolated from	floor dust of a hemp factory
[Ref.: #11794]	Geographic location (country and/or sea, region)	Lucknow
[Ref.: #11794]	Country	India
[Ref.: #11794]	Country ISO 3 Code	IND
[Ref.: #11794]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Floor dust of a hemp factory
[Ref.: #67770]	Geographic location (country and/or sea, region)	Lucknow
[Ref.: #67770]	Country	India
[Ref.: #67770]	Country ISO 3 Code	IND
[Ref.: #67770]	Continent	Asia

[Ref.: #122329]	Sample type/isolated from	Environment, Floor dust of a hemp factory
[Ref.: #122329]	Geographic location (country and/or sea, region)	Lucknow
[Ref.: #122329]	Country	India
[Ref.: #122329]	Country ISO 3 Code	IND
[Ref.: #122329]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Engineered	#Industrial	#Plant (Factory)
#Engineered	#Waste	#Dust (Ash)

What are isolation sources categories?

Global distribution of 16S sequence AF223354 (>99% sequence identity) for Amycolatopsis from Microbeatlas

Aquatic Samples	14
Soil Samples	48
Animal Samples	26
Plant Samples	3
Total Samples	91

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #11794]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #19670]	Biosafety level	1	Risk group (German classification)
[Ref.: #122329]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Amycolatopsis sacchari gene for 16S rRNA, partial sequence, strain: NBRC 100339	AB327252	1445	GenBank ENA	115433 tax ID
[Ref.: #11794]	Amycolatopsis sacchari 16S ribosomal RNA gene, partial sequence	AF223354	1484	GenBank ENA	115433 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Amycolatopsis sacchari DSM 44468	GCA_900114035	contig	GenBank ENA	115433 tax ID
[Ref.: #66792]	Amycolatopsis sacchari strain DSM 44468	115433.3	wgs	PATRIC	115433 tax ID	*
[Ref.: #66792]	Amycolatopsis sacchari DSM 44468	2675903226	draft	JGI IMG/M	115433 tax ID	*

GC-content

71.3 mol%

genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Amycolatopsis sacchari strain DSM 44468 PATRIC: 115433.3
[Ref.: #69481]	spore-forming	spore-formingⓘ	yes	99	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Amycolatopsis sacchari strain DSM 44468 PATRIC: 115433.3
[Ref.: #69480]	gram-positive	gram-positiveⓘ	yes	91.666	no
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	96.203	yes
[Ref.: #69480]	spore-forming	spore-formingⓘ	yes	70.481	yes
[Ref.: #69480]	aerobic	aerobicⓘ	yes	88.753	no
[Ref.: #69480]	thermophilic	thermophileⓘ	no	94.994	no
[Ref.: #69480]	flagellated	motile2+ⓘ	no	86.6	no

Availability in culture collections External links

Culture collection no.

DSM 44468, JCM 11272, CIP 107029, KCTC 9863, NBRC 100339, NCIMB 13786

SI-ID 50638

*

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Amycolatopsis anabasis sp. nov., a novel endophytic actinobacterium isolated from roots of Anabasis elatior.	Wang HF, Li XY, Gao R, Xie YG, Xiao M, Li QL, Li WJ	Int J Syst Evol Microbiol	10.1099/ijsem.0.004184	2020	*
Phylogeny	Amycolatopsis rhizosphaerae sp. nov., isolated from rice rhizosphere soil.	Thawai C	Int J Syst Evol Microbiol	10.1099/ijsem.0.002704	2018	*
Phylogeny	Amycolatopsis cihanbeyliensis sp. nov., a halotolerant actinomycete isolated from a salt mine.	Tatar D, Sazak A, Guven K, Cetin D, Sahin N	Int J Syst Evol Microbiol	10.1099/ijs.0.050963-0	2013	*
Phylogeny	Amycolatopsis magusensis sp. nov., isolated from soil.	Camas M, Sahin N, Sazak A, Sproer C, Klenk HP	Int J Syst Evol Microbiol	10.1099/ijs.0.042770-0	2012	*
Phylogeny	Amycolatopsis bartoniae sp. nov. and Amycolatopsis bullii sp. nov., mesophilic actinomycetes isolated from arid Australian soils.	Zucchi TD, Bonda AN, Frank S, Kim BY, Kshetrimayum JD, Goodfellow M	Antonie Van Leeuwenhoek	10.1007/s10482-012-9716-0	2012	*
Phylogeny	Sciscionella marina gen. nov., sp. nov., a marine actinomycete isolated from a sediment in the northern South China Sea.	Tian XP, Zhi XY, Qiu YQ, Zhang YQ, Tang SK, Xu LH, Zhang S, Li WJ	Int J Syst Evol Microbiol	10.1099/ijs.0.001982-0	2009	*
Phylogeny	Amycolatopsis sacchari sp. nov., a moderately thermophilic actinomycete isolated from vegetable matter.	Goodfellow M, Kim SB, Minnikin DE, Whitehead D, Zhou ZH, Mattinson-Rose AD	Int J Syst Evol Microbiol	10.1099/00207713-51-1-187	2001	*

References

#11794

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 44468

#19670

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#39634 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#66794

Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68368

Automatically annotated from API 20E .

#68371

Automatically annotated from API 50CH acid .

#68379

Automatically annotated from API Coryne .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#82440

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID50638.1 )

#122329 Collection of Institut Pasteur ; Curators of the CIP; CIP 107029
* These data were automatically processed and therefore are not curated

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