Name: Enterococcus rotai strain 1971
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 132142

Type strain:

Species: Enterococcus rotai

Strain Designation: strain 1971

Culture col. no.: DSM 102982, CCM 4630, CCUG 61593, LMG 26678

Strain history: <- I. Sedlácek, CCM; CCM 4630 <- B. Otipka; strain 1971

NCBI tax ID(s): 118060 (species)

SI-ID 66358

CCM 4630, LMG 26678, CCUG 61593, DSM 102982

Section

Enterococcus rotai strain 1971 is a facultative anaerobe, mesophilic, Gram-positive bacterium that was isolated from drinking water.

Gram-positive
ovoid-shaped
facultative anaerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Lactobacillales*
	Family	*Enterococcaceae*
	Genus	*Enterococcus*
	Species	**Enterococcus rotai**
	Full Scientific Name (LPSN)	Enterococcus rotai Sedlá?ek et al. 2013

Information on morphological and physiological properties Morphology

[Ref.: #30651]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #30651]	Cell shape	ovoid-shaped

[Ref.: #30651]	Motility	no
[Ref.: #69480]	Motility	no Confidence in %95.272

[Ref.: #30651]

Pigment production

yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #23932]

Culture medium

TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)

[Ref.: #23932]

Culture medium growth

[Ref.: #23932]

Culture medium link

Medium recipe at MediaDive

[Ref.: #23932]

Culture medium composition

expand / minimize

[Ref.: #23932]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #23932]

Culture medium growth

[Ref.: #23932]

Culture medium link

Medium recipe at MediaDive

[Ref.: #23932]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #23932]	growth	37 °C
[Ref.: #30651]	growth	10-42 °C
[Ref.: #30651]	optimum	26 °C
[Ref.: #62665]	growth	37 °C

[Ref.: #23932]	Temperature range	mesophilic
[Ref.: #30651]	Temperature range	mesophilic
[Ref.: #62665]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #30651]			optimum	9.6

Information on physiology and metabolism Physiology and metabolism

[Ref.: #30651]	Oxygen tolerance	facultative anaerobe
[Ref.: #62665]	Oxygen tolerance	microaerophile

[Ref.: #30651]	Ability of spore formation	no
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.979

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #30651]		NaCl		optimum	6.5	%

[Ref.: #30651]

Observation

aggregates in chains

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68381]	alpha-cyclodextrin ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	arginine ChEBI	+	hydrolysis	* from API rID32STR
[Ref.: #30651]	cellobiose ChEBI	+	carbon source
[Ref.: #68381]	D-arabitol ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	D-mannitol ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	D-ribose ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #68381]	D-tagatose ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #30651]	esculin ChEBI	+	hydrolysis
[Ref.: #30651]	fructose ChEBI	+	carbon source
[Ref.: #30651]	glucose ChEBI	+	carbon source
[Ref.: #30651]	glycerol ChEBI	+	carbon source
[Ref.: #68381]	glycogen ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	hippurate ChEBI	+	hydrolysis	* from API rID32STR
[Ref.: #30651]	inosine ChEBI	+	carbon source
[Ref.: #68381]	L-arabinose ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #30651]	maltose ChEBI	+	carbon source
[Ref.: #68381]	maltose ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #30651]	mannose ChEBI	+	carbon source
[Ref.: #68381]	melezitose ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #68381]	melibiose ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	methyl beta-D-glucopyranoside ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #30651]	methyl D-glucoside ChEBI	+	carbon source
[Ref.: #30651]	methyl pyruvate ChEBI	+	carbon source
[Ref.: #30651]	N-acetylglucosamine ChEBI	+	carbon source
[Ref.: #68381]	pullulan ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #30651]	salicin ChEBI	+	carbon source
[Ref.: #68381]	sorbitol ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #30651]	sucrose ChEBI	+	carbon source
[Ref.: #68381]	sucrose ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #30651]	thymidine ChEBI	+	carbon source
[Ref.: #30651]	trehalose ChEBI	+	carbon source
[Ref.: #68381]	trehalose ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #68381]	urea ChEBI	+	hydrolysis	* from API rID32STR
[Ref.: #30651]	uridine ChEBI	+	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #68381]		acetoin ChEBI	yes	* from API rID32STR

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #68381]		acetoin ChEBI	+	* from API rID32STR

	Enzyme	Enzyme activity	EC number
[Ref.: #30651]	acid phosphatase	+	3.1.3.2
[Ref.: #68381]	Alanyl-Phenylalanyl-Proline arylamidase	-		* from API rID32STR
[Ref.: #68381]	alpha-galactosidase	+	3.2.1.22	* from API rID32STR
[Ref.: #68381]	arginine dihydrolase	+	3.5.3.6	* from API rID32STR
[Ref.: #68381]	beta-galactosidase	-	3.2.1.23	* from API rID32STR
[Ref.: #68381]	beta-glucosidase	+	3.2.1.21	* from API rID32STR
[Ref.: #68381]	beta-glucuronidase	-	3.2.1.31	* from API rID32STR
[Ref.: #68381]	beta-mannosidase	+	3.2.1.25	* from API rID32STR
[Ref.: #68381]	glycyl tryptophan arylamidase	+		* from API rID32STR
[Ref.: #68381]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API rID32STR
[Ref.: #68381]	pyrrolidonyl arylamidase	+	3.4.19.3	* from API rID32STR
[Ref.: #68381]	urease	+	3.5.1.5	* from API rID32STR
	Only first 10 entries are displayed. Click here to see all.

API® rID32STR:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Arginine dihydrolaseADH (Arg)	beta-Glucosidasebeta GLU	beta-Galactosidasebeta GAR	beta-Glucuronidasebeta GUR	alpha-Galactosidasealpha GAL	Alkaline PhosphatasePAL	Acid from D-riboseRIB	Acid from D-mannitolMAN	Acid from sorbitolSOR	Acid from lactoseLAC	Acid from trehaloseTRE	Acid from raffinoseRAF	Acid from sucroseSAC	Acid from L-arabinoseLARA	Acid from D-arabitolDARL	Acid from alpha-cyclodextrinCDEX	Acetoin production (Voges Proskauer test)VP	Alanyl-Phenylalanyl-Proline arylamidaseAPPA	beta-Galactosidasebeta GAL	Pyrrolidonyl arylamidasePyrA	N-Acetyl-glucosaminidasebeta NAG	Glycyl-tryptophan arylamidaseGTA	Hydrolysis of hippuric acidHIP	Acid from glycogenGLYG	Acid from pullulanPUL	Acid from maltoseMAL	Acid from melibioseMEL	Acid from melezitoseMLZ	Acidification of methyl beta-D-glucopyranosideMbeta DG	Acid from D-tagatoseTAG	beta-Mannosidasebeta MAN	Urease/urea hydrolysisURE
[Ref.: #23932]	10719	+	+	-	-	+	-	+	-	-	-	+	-	+	-	-	-	+	-	-	+	+	+	+	-	-	+	-	+	+	-	+	+
[Ref.: #23932]	10883	+	+	-	-	+	+/-	+	-	-	+	+	+	+	-	-	-	+	-	-	+	+	+	+	-	-	+	-	+	+	-	+	+

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #23932]	Sample type/isolated from	drinking water
[Ref.: #23932]	Geographic location (country and/or sea, region)	Jablunkov
[Ref.: #23932]	Country	Czech Republic
[Ref.: #23932]	Country ISO 3 Code	CZE
[Ref.: #23932]	Continent	Europe
[Ref.: #23932]	Geographic location	49.5713°/18.7597°

[Ref.: #62665]	Sample type/isolated from	Drinking water
[Ref.: #62665]	Sampling date	1995
[Ref.: #62665]	Geographic location (country and/or sea, region)	Moravia,Jablunkov
[Ref.: #62665]	Country	Czech Republic
[Ref.: #62665]	Country ISO 3 Code	CZE
[Ref.: #62665]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Environmental

#Aquatic

#Freshwater

What are isolation sources categories?

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #23932]	Enterococcus rotai 16S rRNA gene, strain CCM4630	AJ276353	1514	ENA	118060 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Enterococcus rotai LMG 26678	GCA_001465345	chromosome	GenBank	118060 tax ID	*
[Ref.: #66792]	Enterococcus rotai strain LMG 26678	118060.4	complete	PATRIC	118060 tax ID	*
[Ref.: #66792]	Enterococcus rotai DSM 102982	2928218588	draft	JGI IMG/M	118060 tax ID	*
[Ref.: #66792]	Enterococcus rotai LMG 26678	2687453606	complete	JGI IMG/M	118060 tax ID	*

[Ref.: #23932]

GC-content

36.2 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Enterococcus rotai LMG 26678 NCBI: GCA_001465345.1
[Ref.: #69481]	spore-forming	no	50	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Enterococcus rotai strain LMG 26678 PATRIC: 118060.4
[Ref.: #69480]	motile	no	94.247	no
[Ref.: #69480]	gram-positive	yes	91.464	no
[Ref.: #69480]	anaerobic	no	92.914	yes
[Ref.: #69480]	aerobic	no	92.815	no
[Ref.: #69480]	halophile	yes	84.989	yes
[Ref.: #69480]	spore-forming	no	89.328	yes
[Ref.: #69480]	flagellated	no	96.964	yes
[Ref.: #69480]	thermophile	no	99.212	yes
[Ref.: #69480]	glucose-util	yes	87.753	yes
[Ref.: #69480]	glucose-ferment	yes	80.842	no

Availability in culture collections External links

[Ref.: #23932]

Culture collection no.

DSM 102982, CCM 4630, CCUG 61593, LMG 26678

[Ref.: #90498]

SI-ID 66358

Literature:

Topic	Title	Authors	Journal	DOI	Year
Genetics	Complete Genome Sequences of Enterococcus rotai LMG 26678T and Enterococcus silesiacus LMG 23085T.	Lauer AC, Humrighouse BW, Loparev V, Shewmaker PL, Whitney AM, McQuiston JR, McLaughlin RW	Genome Announc	10.1128/genomeA.01387-16	2016	*
Phylogeny	Enterococcus ureilyticus sp. nov. and Enterococcus rotai sp. nov., two urease-producing enterococci from the environment.	Sedlacek I, Holochova P, Maslanova I, Kosina M, Sproer C, Bryndova H, Vandamme P, Rudolf I, Hubalek Z, Svec P	Int J Syst Evol Microbiol	10.1099/ijs.0.041152-0	2012	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#23932

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 102982

#30651

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26982 (see below)

#62665 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 61593

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68381

Automatically annotated from API rID32STR .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#90498

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID66358.1 )

#26982

IJSEM 502 2013 ( DOI 10.1099/ijs.0.041152-0 , PubMed 22523160 )

* These data were automatically processed and therefore are not curated

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Enterococcus rotai LMG 26678

Enterococcus rotai strain LMG 26678

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