Name: Actinokineospora diospyrosa DSM 44255
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 13199

Type strain:

Species: Actinokineospora diospyrosa

Culture col. no.: DSM 44255, IFO 15665, JCM 9921, KCTC 9569, MTCC 2687, NBRC 15665, IMSNU 22098, NCIMB 13657, NRRL B-24047, VKM Ac-1984

Strain history: IFO 15665 <-- T. Tamura YU8-1 <-- M. Hayakawa.

NCBI tax ID(s): 103728 (species)

SI-ID 50537

DSM 44255, IFO 15665, JCM 9921, KCTC 9569, NCIMB 13657, VKM Ac-1984, NRRL B-24047, NBRC 15665

Section

Actinokineospora diospyrosa DSM 44255 is an aerobe, mesophilic, Gram-positive bacterium that was isolated from fallen persimmon leaves.

Gram-positive
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Pseudonocardiales*
	Family	*Pseudonocardiaceae*
	Genus	*Actinokineospora*
	Species	**Actinokineospora diospyrosa**
	Full Scientific Name (LPSN)	Actinokineospora diospyrosa Tamura et al. 1995

Information on morphological and physiological properties Morphology

[Ref.: #67771]	Gram stain	positive

[Ref.: #19639]	Colony color	Red brown
[Ref.: #19639]	Incubation period	10-14 days
[Ref.: #19639]	Cultivation medium used	ISP 2

[Ref.: #19639]	Colony color	Ivory
[Ref.: #19639]	Incubation period	10-14 days
[Ref.: #19639]	Cultivation medium used	ISP 3

[Ref.: #19639]	Colony color	Sand yellow
[Ref.: #19639]	Incubation period	10-14 days
[Ref.: #19639]	Cultivation medium used	ISP 4

[Ref.: #19639]	Colony color	Sand yellow
[Ref.: #19639]	Incubation period	10-14 days
[Ref.: #19639]	Cultivation medium used	ISP 5

[Ref.: #19639]	Colony color	Light ivory
[Ref.: #19639]	Incubation period	10-14 days
[Ref.: #19639]	Cultivation medium used	ISP 6

[Ref.: #19639]	Colony color	Ivory
[Ref.: #19639]	Incubation period	10-14 days
[Ref.: #19639]	Cultivation medium used	ISP 7

[Ref.: #19639]	Multicellular complex forming ability	no
[Ref.: #19639]	Medium Name (multicellularity)	ISP 2

[Ref.: #19639]	Multicellular complex forming ability	no
[Ref.: #19639]	Medium Name (multicellularity)	ISP 3

[Ref.: #19639]	Multicellular complex forming ability	no
[Ref.: #19639]	Medium Name (multicellularity)	ISP 4

[Ref.: #19639]	Multicellular complex forming ability	no
[Ref.: #19639]	Medium Name (multicellularity)	ISP 5

[Ref.: #19639]	Multicellular complex forming ability	no
[Ref.: #19639]	Medium Name (multicellularity)	ISP 6

[Ref.: #19639]	Multicellular complex forming ability	no
[Ref.: #19639]	Medium Name (multicellularity)	ISP 7

Information on culture and growth conditions Culture and growth conditions

[Ref.: #11627]

Culture medium

GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)

[Ref.: #11627]

Culture medium growth

[Ref.: #11627]

Culture medium link

Medium recipe at MediaDive

[Ref.: #11627]

Culture medium composition

expand / minimize

[Ref.: #19639]

Culture medium

ISP 2

[Ref.: #19639]

Culture medium growth

[Ref.: #19639]

Culture medium composition

expand / minimize

[Ref.: #19639]

Culture medium

ISP 3

[Ref.: #19639]

Culture medium growth

[Ref.: #19639]

Culture medium composition

expand / minimize

[Ref.: #19639]

Culture medium

ISP 4

[Ref.: #19639]

Culture medium growth

[Ref.: #19639]

Culture medium composition

expand / minimize

[Ref.: #19639]

Culture medium

ISP 5

[Ref.: #19639]

Culture medium growth

[Ref.: #19639]

Culture medium composition

expand / minimize

[Ref.: #19639]

Culture medium

ISP 6

[Ref.: #19639]

Culture medium growth

[Ref.: #19639]

Culture medium composition

expand / minimize

[Ref.: #19639]

Culture medium

ISP 7

[Ref.: #19639]

Culture medium growth

[Ref.: #19639]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #11627]	growth	28 °C
[Ref.: #67770]	growth	28 °C
[Ref.: #67771]	growth	28 °C

[Ref.: #11627]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #67771]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #67771]

Oxygen tolerance

aerobe

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #19639]		NaCl		maximum	2.5	%

[Ref.: #67770]	Observation	quinones: MK-9(H₄)
[Ref.: #67771]	Observation	quinones: MK-9(H_4_)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #19639]	arabinose ChEBI	-
[Ref.: #68368]	arginine ChEBI	+	hydrolysis	* from API 20E
[Ref.: #19639]	cellulose ChEBI	-
[Ref.: #68368]	citrate ChEBI	+	assimilation	* from API 20E
[Ref.: #19639]	fructose ChEBI	-
[Ref.: #68368]	gelatin ChEBI	+	hydrolysis	* from API 20E
[Ref.: #19639]	glucose ChEBI	+
[Ref.: #68368]	lysine ChEBI	+	degradation	* from API 20E
[Ref.: #19639]	mannitol ChEBI	-
[Ref.: #19639]	myo-inositol ChEBI	-
[Ref.: #68368]	ornithine ChEBI	+	degradation	* from API 20E
[Ref.: #19639]	raffinose ChEBI	-
[Ref.: #19639]	rhamnose ChEBI	-
[Ref.: #19639]	sucrose ChEBI	+
[Ref.: #68368]	tryptophan ChEBI	-	energy source	* from API 20E
[Ref.: #68368]	urea ChEBI	+	hydrolysis	* from API 20E
[Ref.: #19639]	xylose ChEBI	-
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #68368]	acetoin ChEBI	yes	* from API 20E
[Ref.: #68368]	hydrogen sulfide ChEBI	no	* from API 20E
[Ref.: #68368]	indole ChEBI	no	* from API 20E

	Physiological tests	Metabolite	Voges-Proskauer-test	Indole test
[Ref.: #68368]		acetoin ChEBI	+		* from API 20E
[Ref.: #68368]		indole ChEBI		-	* from API 20E

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68368]	arginine dihydrolase	+	3.5.3.6	* from API 20E
[Ref.: #68368]	beta-galactosidase	-	3.2.1.23	* from API 20E, API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68368]	gelatinase	+		* from API 20E
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	+		* from API zym
[Ref.: #68368]	lysine decarboxylase	+	4.1.1.18	* from API 20E
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	-		* from API zym
[Ref.: #68368]	ornithine decarboxylase	+	4.1.1.17	* from API 20E
[Ref.: #68382]	trypsin	+	3.4.21.4	* from API zym
[Ref.: #68368]	tryptophan deaminase	-	4.1.99.1	* from API 20E
[Ref.: #68368]	urease	+	3.5.1.5	* from API 20E
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 20E:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	beta-GalactosidaseONPG	Arginine dihydrolaseADH (Arg)	Lysine decarboxylaseLDC (Lys)	Ornithine decarboxylaseODC	Citrate utilizationCIT	H2S productionH2S	Urease/urea hydrolysisURE	Tryptophan deaminaseTDA (Trp)	Indole productionIND	Acetoin production (Voges Proskauer test)VP	Gelatinase/gelatin hydrolysisGEL	Fermentation/oxidation of D-glucoseGLU	Fermentation/oxidation of D-mannitolMAN	Fermentation/oxidation of inositolINO	Fermentation/oxidation of sorbitolSor	Fermentation/oxidation of L-rhamnoseRHA	Fermentation/oxidation of sucroseSAC	Fermentation/oxidation of melibioseMEL	Fermentation/oxidation of amygdalinAMY	Fermentation/oxidation of L-arabinoseARA	Cytochrome oxidaseOX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	Oxidative catabolisation of D-glucoseOF-O	Fermentation of D-glucoseOF-F
[Ref.: #19639]	478	-	+	+	+	+	-	+	-	-	+	+	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #19639]	466	not determinedn.d.	+	+	+	+	+	+	-	+	-	+	-	-	-	-	+	-	+	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #11627]	Sample type/isolated from	fallen persimmon leaves
[Ref.: #11627]	Geographic location (country and/or sea, region)	Yamanashi prefecture
[Ref.: #11627]	Country	Japan
[Ref.: #11627]	Country ISO 3 Code	JPN
[Ref.: #11627]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Fallen persimmon leaves
[Ref.: #67770]	Geographic location (country and/or sea, region)	Yamanashi Pref.
[Ref.: #67770]	Country	Japan
[Ref.: #67770]	Country ISO 3 Code	JPN
[Ref.: #67770]	Continent	Asia

[Ref.: #67771]	Sample type/isolated from	From fallen persimmon leaves
[Ref.: #67771]	Geographic location (country and/or sea, region)	Yamanashi Pref.
[Ref.: #67771]	Country	Japan
[Ref.: #67771]	Country ISO 3 Code	JPN
[Ref.: #67771]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Host	#Plants	#Tree
#Host Body-Site	#Plant	#Leaf (Phyllosphere)

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #11627]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #19639]	Biosafety level	1	Risk group (German classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Actinokineospora diospyrosa strain NRRL B-24047T 16S ribosomal RNA gene, partial sequence	AF114797	1512	ENA	103728 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Actinokineospora diospyrosa DSM 44255	2596583538	draft	JGI IMG/M	103728 tax ID	*
[Ref.: #66792]	Actinokineospora diospyrosa DSM 44255	GCA_024171925	scaffold	GenBank	103728 tax ID	*

[Ref.: #11627]	GC-content	69.3 mol%
[Ref.: #67770]	GC-content	69.3 mol%	high performance liquid chromatography (HPLC)
[Ref.: #67770]	GC-content	70.3 mol%	genome sequence analysis

Availability in culture collections External links

[Ref.: #11627]

Culture collection no.

DSM 44255, IFO 15665, JCM 9921, KCTC 9569, MTCC 2687, NBRC 15665, IMSNU 22098, NCIMB 13657, NRRL B-24047, VKM Ac-1984

[Ref.: #82407]

SI-ID 50537

References

#11627

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 44255

#19639

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#68368

Automatically annotated from API 20E .

#68382

Automatically annotated from API zym .

#82407

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID50537.1 )

* These data were automatically processed and therefore are not curated

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