Name: Leuconostoc rapi R-50029
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 131396

Type strain:

Species: Leuconostoc rapi

Strain Designation: R-50029

Culture col. no.: DSM 27776, LMG 27676, MHB 277, CIP 110986

Strain history: CIP <- 2015,BCCM LMG <- 2013, I. Snauwaert, Ghent Univ., Ghent, Belgium: strain R-50029 <- S. Pihlajaviita, Seinajoki Univ., Seinajoki, Finland: strain MHB 277

NCBI tax ID(s): 1406906 (species)

SI-ID 390521

LMG 27676, DSM 27776

Section

Leuconostoc rapi R-50029 is a microaerophile, mesophilic, Gram-positive bacterium that was isolated from spoiled sous-vide-cooked rutabaga .

Gram-positive
mesophilic
microaerophile
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Lactobacillales*
	Family	*Lactobacillaceae*
	Genus	*Leuconostoc*
	Species	**Leuconostoc rapi**
	Full Scientific Name (LPSN)	Leuconostoc rapi Lyhs et al. 2015

Information on morphological and physiological properties Morphology

[Ref.: #122138]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #122138]	Cell shape	oval-shaped

[Ref.: #122138]	Motility	no
[Ref.: #69480]	Motility	no Confidence in %96.998

Information on culture and growth conditions Culture and growth conditions

[Ref.: #22623]

Culture medium

MRS MEDIUM (DSMZ Medium 11)

[Ref.: #22623]

Culture medium growth

[Ref.: #22623]

Culture medium link

Medium recipe at MediaDive

[Ref.: #22623]

Culture medium composition

expand / minimize

[Ref.: #122138]

Culture medium

CIP Medium 40

[Ref.: #122138]

Culture medium growth

[Ref.: #122138]

Culture medium link

Medium recipe provided by DSMZ

	Temperatures	Kind of temperature		Temperature
[Ref.: #22623]			growth	30 °C

[Ref.: #22623]

Temperature range

mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #22623]

Oxygen tolerance

microaerophile

[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %100

[Ref.: #22623]	Murein short key	A11.05
[Ref.: #22623]	Murein types	A3alpha L-Lys-L-Ala₂

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68371]	amygdalin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	arbutin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	cellobiose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fructose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-galactose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-glucose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-lyxose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-ribose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-sorbitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-tagatose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	esculin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gentiobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycerol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycogen ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	inulin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-rhamnose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-sorbose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	lactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	maltose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	melezitose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	melibiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-glucopyranoside ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-mannoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	myo-inositol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	N-acetylglucosamine ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	Potassium 2-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	Potassium 5-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	raffinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	salicin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	starch ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	sucrose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	trehalose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	turanose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	xylitol ChEBI	-	builds acid from	* from API 50CH acid
	Only first 10 entries are displayed. Click here to see all.

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #22623]	14833	-	-	-	-	-	-	-	-	-	-	+	+	+	-	-	-	-	-	-	-	-	+	+	+	-	+	+	+	-	-	-	+	+	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #22623]	Sample type/isolated from	spoiled sous-vide-cooked rutabaga (Brassica napobrassica)
[Ref.: #22623]	Host species	Brassica napobrassica
[Ref.: #22623]	Country	Finland
[Ref.: #22623]	Country ISO 3 Code	FIN
[Ref.: #22623]	Continent	Europe

[Ref.: #122138]	Sample type/isolated from	Spoiled packed sous-vide cooked rutabaga, Brassica napobrassica
[Ref.: #122138]	Country	Finland
[Ref.: #122138]	Country ISO 3 Code	FIN
[Ref.: #122138]	Continent	Europe
[Ref.: #122138]	Isolation date	2012-01-02
* marker position based on {}

Isolation sources categories

#Host	#Plants	#Decomposing plant
#Host	#Plants	#Herbaceous plants (Grass,Crops)

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #22623]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #122138]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #22623]	Leuconostoc rapi partial 16S rRNA gene, type strain LMG 27676T	HG515542	1502	ENA	1406906 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Leuconostoc rapi DSM 27776	GCA_016908715	contig	GenBank	1406906 tax ID	*
[Ref.: #66792]	Leuconostoc rapi DSM 27776	GCA_019656095	contig	GenBank	1406906 tax ID	*
[Ref.: #66792]	Leuconostoc rapi strain DSM 27776	1406906.5	wgs	PATRIC	1406906 tax ID	*
[Ref.: #66792]	Leuconostoc rapi strain DSM 27776	1406906.3	wgs	PATRIC	1406906 tax ID	*
[Ref.: #66792]	Leuconostoc rapi DSM 27776	2901184053	draft	JGI IMG/M	1406906 tax ID	*

[Ref.: #22623]

GC-content

38 mol%

high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Leuconostoc rapi strain DSM 27776 PATRIC: 1406906.3
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Leuconostoc rapi strain DSM 27776 PATRIC: 1406906.5
[Ref.: #69480]	motile	no	95.328	no
[Ref.: #69480]	gram-positive	yes	96.991	no
[Ref.: #69480]	anaerobic	no	93.64	yes
[Ref.: #69480]	halophile	yes	95.838	no
[Ref.: #69480]	spore-forming	no	94.872	no
[Ref.: #69480]	thermophile	no	99.754	yes
[Ref.: #69480]	glucose-util	yes	88.927	no
[Ref.: #69480]	aerobic	no	97.095	no
[Ref.: #69480]	flagellated	no	97.785	no
[Ref.: #69480]	glucose-ferment	yes	91.22	no

Availability in culture collections External links

[Ref.: #22623]

Culture collection no.

DSM 27776, LMG 27676, MHB 277, CIP 110986

[Ref.: #89829]

SI-ID 390521

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Leuconostoc rapi sp. nov., isolated from sous-vide-cooked rutabaga.	Lyhs U, Snauwaert I, Pihlajaviita S, Vuyst L, Vandamme P	Int J Syst Evol Microbiol	10.1099/ijs.0.000305	2015	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#22623

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 27776

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68371

Automatically annotated from API 50CH acid .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#89829

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID390521.1 )

#122138 Collection of Institut Pasteur ; Curators of the CIP; CIP 110986
* These data were automatically processed and therefore are not curated

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Leuconostoc rapi strain DSM 27776

Leuconostoc rapi strain DSM 27776

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