Secundilactobacillus mixtipabuli (DSM 28580, JCM 19805, IWT30)

Name: Secundilactobacillus mixtipabuli (DSM 28580, JCM 19805, IWT30)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 130383

Type strain

Strain Designation IWT30

Culture col. no. DSM 28580 JCM 19805 IWT30 LMG 30915 BCRC 81166

NCBI tax ID(s) 1435342

Special Collections DSMZ JCM

History DSMZ <- M. Tohno, National Agriculture and Food Res. Org. (NARO), Nat. Inst. of Livestock and Grassland Science, Tochigi, Japan; IWT30 JCM <-- M. Tohno IWT30.

Links

Secundilactobacillus mixtipabuli IWT30 is a microaerophile bacterium that was isolated from total mixed ration silage formulated with with grass silage, corn silage, soybean curd residue, hay, compound feed and and mixed feed.

microaerophile genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 405997

LMG 30915,JCM 19805,DSM 28580

@ref 20215

Last LPSN update 2026-05-29 08:37:22

Domain Bacteria

Phylum Bacillota

Class Bacilli

Order Lactobacillales

Family Lactobacillaceae

Genus Secundilactobacillus

Species Secundilactobacillus mixtipabuli

Full scientific name Secundilactobacillus mixtipabuli (Tohno et al. 2015) Zheng et al. 2020

Synonyms (1)

Lactobacillus mixtipabuli

BacDive ID	Other strains from **Secundilactobacillus mixtipabuli** (2)	Type strain
162616	S. mixtipabuli JCM 19804
162617	S. mixtipabuli JCM 19806

Morphology

Cell morphology

@ref	Motility	Confidence	Gram stain
125438		90
125438		93.503	positive

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
21919	MRS MEDIUM (DSMZ Medium 11)		Medium recipe at MediaDive	Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
21919	positive	growth	30
67770	positive	growth	30

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
21919	microaerophile
125439	facultative anaerobe	92.408

Spore formation

@ref	Spore formation	Confidence
125439		93.585

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68371	27613 ChEBI	amygdalin	-	builds acid from	from API 50CH acid
68371	18305 ChEBI	arbutin	-	builds acid from	from API 50CH acid
68371	17057 ChEBI	cellobiose	-	builds acid from	from API 50CH acid
68371	17108 ChEBI	D-arabinose	-	builds acid from	from API 50CH acid
68371	18333 ChEBI	D-arabitol	-	builds acid from	from API 50CH acid
68371	15824 ChEBI	D-fructose	-	builds acid from	from API 50CH acid
68371	28847 ChEBI	D-fucose	-	builds acid from	from API 50CH acid
68371	12936 ChEBI	D-galactose	-	builds acid from	from API 50CH acid
68371	17634 ChEBI	D-glucose	+	builds acid from	from API 50CH acid
68371	62318 ChEBI	D-lyxose	-	builds acid from	from API 50CH acid
68371	16899 ChEBI	D-mannitol	-	builds acid from	from API 50CH acid
68371	16024 ChEBI	D-mannose	-	builds acid from	from API 50CH acid
68371	16988 ChEBI	D-ribose	+	builds acid from	from API 50CH acid
68371	17924 ChEBI	D-sorbitol	-	builds acid from	from API 50CH acid
68371	16443 ChEBI	D-tagatose	-	builds acid from	from API 50CH acid
68371	65327 ChEBI	D-xylose	+	builds acid from	from API 50CH acid
68371	17113 ChEBI	erythritol	-	builds acid from	from API 50CH acid
68371	4853 ChEBI	esculin	-	builds acid from	from API 50CH acid
68371	16813 ChEBI	galactitol	-	builds acid from	from API 50CH acid
68371	28066 ChEBI	gentiobiose	-	builds acid from	from API 50CH acid
68371	24265 ChEBI	gluconate	-	builds acid from	from API 50CH acid
68371	17754 ChEBI	glycerol	-	builds acid from	from API 50CH acid
68371	28087 ChEBI	glycogen	-	builds acid from	from API 50CH acid
68371	15443 ChEBI	inulin	-	builds acid from	from API 50CH acid
68371	30849 ChEBI	L-arabinose	+	builds acid from	from API 50CH acid
68371	18403 ChEBI	L-arabitol	-	builds acid from	from API 50CH acid
68371	18287 ChEBI	L-fucose	-	builds acid from	from API 50CH acid
68371	62345 ChEBI	L-rhamnose	-	builds acid from	from API 50CH acid
68371	17266 ChEBI	L-sorbose	-	builds acid from	from API 50CH acid
68371	65328 ChEBI	L-xylose	-	builds acid from	from API 50CH acid
68371	17716 ChEBI	lactose	-	builds acid from	from API 50CH acid
68371	17306 ChEBI	maltose	+	builds acid from	from API 50CH acid
68371	6731 ChEBI	melezitose	-	builds acid from	from API 50CH acid
68371	28053 ChEBI	melibiose	-	builds acid from	from API 50CH acid
68371	320061 ChEBI	methyl alpha-D-glucopyranoside	-	builds acid from	from API 50CH acid
68371	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from	from API 50CH acid
68371	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from	from API 50CH acid
68371	17268 ChEBI	myo-inositol	-	builds acid from	from API 50CH acid
68371	59640 ChEBI	N-acetylglucosamine	+	builds acid from	from API 50CH acid
68371	0 ChEBI	Potassium 2-ketogluconate	-	builds acid from	from API 50CH acid
68371	0 ChEBI	Potassium 5-ketogluconate	-	builds acid from	from API 50CH acid
68371	16634 ChEBI	raffinose	-	builds acid from	from API 50CH acid
68371	15963 ChEBI	ribitol	-	builds acid from	from API 50CH acid
68371	17814 ChEBI	salicin	-	builds acid from	from API 50CH acid
68371	28017 ChEBI	starch	-	builds acid from	from API 50CH acid
68371	17992 ChEBI	sucrose	-	builds acid from	from API 50CH acid
68371	27082 ChEBI	trehalose	-	builds acid from	from API 50CH acid
68371	32528 ChEBI	turanose	-	builds acid from	from API 50CH acid
68371	17151 ChEBI	xylitol	-	builds acid from	from API 50CH acid

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions
66794	CDP-diacylglycerol biosynthesis	100	2 of 2
66794	L-lactaldehyde degradation	100	3 of 3
66794	acetate fermentation	100	4 of 4
66794	coenzyme A metabolism	100	4 of 4
66794	teichoic acid biosynthesis	100	1 of 1
66794	ppGpp biosynthesis	100	4 of 4
66794	formaldehyde oxidation	100	3 of 3
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	cis-vaccenate biosynthesis	100	2 of 2
66794	methylglyoxal degradation	100	5 of 5
66794	palmitate biosynthesis	100	22 of 22
66794	folate polyglutamylation	100	1 of 1
66794	anapleurotic synthesis of oxalacetate	100	1 of 1
66794	suberin monomers biosynthesis	100	2 of 2
66794	vitamin B1 metabolism	92.31	12 of 13
66794	threonine metabolism	90	9 of 10
66794	aspartate and asparagine metabolism	88.89	8 of 9
66794	chorismate metabolism	88.89	8 of 9
66794	pentose phosphate pathway	81.82	9 of 11
66794	peptidoglycan biosynthesis	80	12 of 15
66794	lipoate biosynthesis	80	4 of 5
66794	molybdenum cofactor biosynthesis	77.78	7 of 9
66794	CO2 fixation in Crenarchaeota	77.78	7 of 9
66794	purine metabolism	77.66	73 of 94
66794	pyrimidine metabolism	75.56	34 of 45
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	75	6 of 8
66794	sulfopterin metabolism	75	3 of 4
66794	flavin biosynthesis	73.33	11 of 15
66794	cardiolipin biosynthesis	71.43	5 of 7
66794	Entner Doudoroff pathway	70	7 of 10
66794	phenylalanine metabolism	69.23	9 of 13
66794	glutamate and glutamine metabolism	67.86	19 of 28
66794	acetoin degradation	66.67	2 of 3
66794	valine metabolism	66.67	6 of 9
66794	serine metabolism	66.67	6 of 9
66794	methane metabolism	66.67	2 of 3
66794	NAD metabolism	66.67	12 of 18
66794	glycolate and glyoxylate degradation	66.67	4 of 6
66794	glycolysis	64.71	11 of 17
66794	photosynthesis	64.29	9 of 14
66794	d-xylose degradation	63.64	7 of 11
66794	proline metabolism	63.64	7 of 11
66794	non-pathway related	63.16	24 of 38
66794	isoleucine metabolism	62.5	5 of 8
66794	ketogluconate metabolism	62.5	5 of 8
66794	degradation of pentoses	60.71	17 of 28
66794	glycine betaine biosynthesis	60	3 of 5
66794	3-chlorocatechol degradation	60	3 of 5
66794	glycogen metabolism	60	3 of 5
66794	degradation of aromatic, nitrogen containing compounds	58.33	7 of 12
66794	propanol degradation	57.14	4 of 7
66794	ubiquinone biosynthesis	57.14	4 of 7
66794	tetrahydrofolate metabolism	57.14	8 of 14
66794	arginine metabolism	54.17	13 of 24
66794	oxidative phosphorylation	52.75	48 of 91
66794	histidine metabolism	51.72	15 of 29
66794	tryptophan metabolism	50	19 of 38
66794	ethanol fermentation	50	1 of 2
66794	CMP-KDO biosynthesis	50	2 of 4
66794	adipate degradation	50	1 of 2
66794	degradation of sugar alcohols	50	8 of 16
66794	C4 and CAM-carbon fixation	50	4 of 8
66794	citric acid cycle	50	7 of 14
66794	butanoate fermentation	50	2 of 4
66794	lipid metabolism	48.39	15 of 31
66794	alanine metabolism	48.28	14 of 29
66794	degradation of sugar acids	48	12 of 25
66794	lysine metabolism	47.62	20 of 42
66794	methionine metabolism	46.15	12 of 26
66794	urea cycle	46.15	6 of 13
66794	isoprenoid biosynthesis	46.15	12 of 26
66794	d-mannose degradation	44.44	4 of 9
66794	allantoin degradation	44.44	4 of 9
66794	reductive acetyl coenzyme A pathway	42.86	3 of 7
66794	glutathione metabolism	42.86	6 of 14
66794	mevalonate metabolism	42.86	3 of 7
66794	metabolism of amino sugars and derivatives	40	2 of 5
66794	gallate degradation	40	2 of 5
66794	glycine metabolism	40	4 of 10
66794	arachidonate biosynthesis	40	2 of 5
66794	propionate fermentation	40	4 of 10
66794	degradation of hexoses	38.89	7 of 18
66794	cysteine metabolism	38.89	7 of 18
66794	gluconeogenesis	37.5	3 of 8
66794	dTDPLrhamnose biosynthesis	37.5	3 of 8
66794	vitamin B6 metabolism	36.36	4 of 11
66794	ascorbate metabolism	36.36	8 of 22
66794	acetyl CoA biosynthesis	33.33	1 of 3
66794	lipid A biosynthesis	33.33	3 of 9
66794	selenocysteine biosynthesis	33.33	2 of 6
66794	enterobactin biosynthesis	33.33	1 of 3
66794	IAA biosynthesis	33.33	1 of 3
66794	sphingosine metabolism	33.33	2 of 6
66794	octane oxidation	33.33	1 of 3
66794	phenol degradation	30	6 of 20
66794	starch degradation	30	3 of 10
66794	tyrosine metabolism	28.57	4 of 14
66794	benzoyl-CoA degradation	28.57	2 of 7
66794	arachidonic acid metabolism	27.78	5 of 18
66794	carnitine metabolism	25	2 of 8
66794	lactate fermentation	25	1 of 4
66794	cyclohexanol degradation	25	1 of 4
66794	glycogen biosynthesis	25	1 of 4
66794	androgen and estrogen metabolism	25	4 of 16
66794	toluene degradation	25	1 of 4
66794	phenylpropanoid biosynthesis	23.08	3 of 13
66794	leucine metabolism	23.08	3 of 13
66794	nitrate assimilation	22.22	2 of 9
66794	4-hydroxymandelate degradation	22.22	2 of 9

API 50CHac

@ref	ControlQ	GLY	ERY	DARA	LARA	RIB	DXYL	LXYL	ADO	MDX	GAL	GLU	FRU	MNE	SBE	RHA	DUL	INO	MAN	SOR	MDM	MDG	NAG	AMY	ARB	ESC	SAL	CEL	MAL	LAC	MEL	SAC	TRE	INU	MLZ	RAF	AMD	GLYG	XLT	GEN	TUR	LYX	TAG	DFUC	LFUC	DARL	LARL	GNT	2KG	5KG
21919	-	-	-	-	+	+	+	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Food production	-
#Host	#Plants	#Herbaceous plants (Grass,Crops)

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
21919	total mixed ration silage formulated with with grass silage, corn silage, soybean curd residue, hay, compound feed and and mixed feed	Iwate, Hachimantai	Japan	JPN	Asia
67770	Total mixed ration silage

Taxonmaps

69479

Global distribution of 16S sequence AB894864 (>99% sequence identity) for Secundilactobacillus from Microbeatlas

Aquatic Samples	237
Soil Samples	155
Animal Samples	1277
Plant Samples	156
Total Samples	1825

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
21919	1	Risk group (German classification)

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	ASM221792v1 assembly for Secundilactobacillus mixtipabuli IWT30	scaffold	GCA_002217925	1435342.4	2956993865	1435342	61.51

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
21919	Lactobacillus mixtipabuli genefor 16S ribosomal RNA, partial sequence, strain: IWT30	AB894864	1488		1435342
124043	Secundilactobacillus mixtipabuli JCM 19805 gene for 16S rRNA, partial sequence.	LC638725	1495		1435342

GC content

@ref	GC-content (mol%)	Method
21919	44.6	high performance liquid chromatography (HPLC)
67770	43.7	genome sequence analysis

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM221792v1 assembly for Secundilactobacillus mixtipabuli IWT30
NCBI: GCA_002217925

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	facultative anaerobe	92.41	no
125439	gram_stain	BacteriaNetⓘ	positive	69.11	no
125439	motility	BacteriaNetⓘ	no	74.63	no
125439	spore_formation	BacteriaNetⓘ	no	93.59	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Secundilactobacillus mixtipabuli IWT30
NCBI: GCA_002217925.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	93.50	no
125438	anaerobic	anaerobicⓘ	no	84.08	no
125438	aerobic	aerobicⓘ	no	94.39	yes
125438	spore-forming	spore-formingⓘ	no	80.83	no
125438	thermophilic	thermophileⓘ	no	95.88	no
125438	flagellated	motile2+ⓘ	no	90.00	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Lactobacillus mixtipabuli sp. nov. isolated from total mixed ration silage.	Tohno M, Kitahara M, Irisawa T, Ohmori H, Masuda T, Ohkuma M, Tajima K	Int J Syst Evol Microbiol	10.1099/ijs.0.000204	2015
Phylogeny	Characterization of two novel pentose-fermenting and GABA-producing species: Levilactobacillus tujiorum sp. nov. and Secundilactobacillus angelensis sp. nov. Isolated from a solid-state fermented zha-chili.	Zhang Z, Wang Y, Dong Y, Xiang F, Zhang Y, Zhang H, Sun Y, Guo Z	Syst Appl Microbiol	10.1016/j.syapm.2022.126344	2022

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#21919	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 28580
#66794	Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68371	Automatically annotated from API 50CH acid .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Secundilactobacillus mixtipabuli (DSM 28580, JCM 19805, IWT30)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Metabolite utilization

Pathway annotation

API 50CHac

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_002217925

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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