Name: Lactobacillus helsingborgensis Bma5N
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 130378

Type strain:

Species: Lactobacillus helsingborgensis

Strain Designation: Bma5N

Culture col. no.: DSM 26265, CCUG 63301, JCM 30766

Strain history: CCUG 63301 <-- T. Olofsson; Lund Univ., Sweden; Bma5N.

NCBI tax ID(s): 1218494 (species)

SI-ID 399143

DSM 26265, CCUG 63301

Other strains from Lactobacillus helsingborgensis

L. helsingborgensis H4bb18N, DSM 26317, CCUG 63635

Section

Lactobacillus helsingborgensis Bma5N is an anaerobe, mesophilic bacterium that was isolated from honey stomach of honey bee .

anaerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Lactobacillales*
	Family	*Lactobacillaceae*
	Genus	*Lactobacillus*
	Species	**Lactobacillus helsingborgensis**
	Full Scientific Name (LPSN)	Lactobacillus helsingborgensis Olofsson et al. 2014

Information on morphological and physiological properties Morphology

[Ref.: #62968]

Incubation period

3 days

Information on culture and growth conditions Culture and growth conditions

[Ref.: #21914]

Culture medium

MRS MEDIUM (DSMZ Medium 11)

[Ref.: #21914]

Culture medium growth

[Ref.: #21914]

Culture medium link

Medium recipe at MediaDive

[Ref.: #21914]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #21914]	growth	35 °C
[Ref.: #62968]	growth	37 °C
[Ref.: #67770]	growth	37 °C

[Ref.: #21914]	Temperature range	mesophilic
[Ref.: #62968]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #21914]	Oxygen tolerance	anaerobe
[Ref.: #62968]	Oxygen tolerance	anaerobe

[Ref.: #21914]	Murein short key	A11.31
[Ref.: #21914]	Murein types	A4alpha L-Lys-D-Asp

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68371]	amygdalin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	arbutin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fructose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-glucose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-lyxose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannitol ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-ribose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-sorbitol ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	esculin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	gentiobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycerol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycogen ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	inulin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-rhamnose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-sorbose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	melezitose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	melibiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-mannoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	myo-inositol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	N-acetylglucosamine ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	Potassium 2-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	Potassium 5-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	raffinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	salicin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	starch ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	sucrose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	trehalose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	turanose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	xylitol ChEBI	-	builds acid from	* from API 50CH acid
	Only first 10 entries are displayed. Click here to see all.

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #21914]	14801	-	-	-	-	-	-	-	-	-	-	+/-	+	+	+	+	-	+/-	-	+	+	-	-	+	-	+	+	+	+/-	+/-	+/-	-	+	+	-	-	-	-	-	-	-	-	-	+/-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #21914]	Sample type/isolated from	honey stomach of honey bee (Apis mellifera mellifera)
[Ref.: #21914]	Host species	Apis mellifera mellifera
[Ref.: #21914]	Geographic location (country and/or sea, region)	Lund
[Ref.: #21914]	Country	Sweden
[Ref.: #21914]	Country ISO 3 Code	SWE
[Ref.: #21914]	Continent	Europe

[Ref.: #62968]	Sample type/isolated from	Honey bee,honey stomach
[Ref.: #62968]	Sampling date	2011-07-06
[Ref.: #62968]	Geographic location (country and/or sea, region)	Helsingborg
[Ref.: #62968]	Country	Sweden
[Ref.: #62968]	Country ISO 3 Code	SWE
[Ref.: #62968]	Continent	Europe

[Ref.: #67770]	Sample type/isolated from	Honey stomach of the honeybee Apis mellifera mellifera
[Ref.: #67770]	Host species	Apis mellifera mellifera
* marker position based on {}

Isolation sources categories

#Host	#Arthropoda	#Insecta
#Host Body-Site	#Gastrointestinal tract	#Stomach

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence JX099553 (>99% sequence identity) for Lactobacillus from Microbeatlas

Aquatic Samples	89
Soil Samples	22
Animal Samples	3293
Plant Samples	66
Total Samples	3470

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #21914]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #21914]	Lactobacillus helsingborgensis strain Bma5N 16S ribosomal RNA gene, partial sequence	JX099553	1438	ENA	1218494 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Lactobacillus helsingborgensis Bma5	GCA_000970855	scaffold	GenBank	1218494 tax ID

[Ref.: #21914]	GC-content	36.3 mol%	sequence analysis
[Ref.: #67770]	GC-content	36.4 mol%	genome sequence analysis
[Ref.: #67770]	GC-content	36.3 mol%	genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Lactobacillus helsingborgensis Bma5 NCBI: GCA_000970855.1
[Ref.: #69480]	motile	no	96.481	no
[Ref.: #69480]	flagellated	no	98.662	no
[Ref.: #69480]	gram-positive	yes	93.034	no
[Ref.: #69480]	anaerobic	yes	93.217	yes
[Ref.: #69480]	aerobic	no	98.275	yes
[Ref.: #69480]	halophile	yes	78.135	no
[Ref.: #69480]	spore-forming	no	97.843	no
[Ref.: #69480]	thermophile	no	96.64	yes
[Ref.: #69480]	glucose-util	yes	91.587	no
[Ref.: #69480]	glucose-ferment	yes	92.784	no

Availability in culture collections External links

[Ref.: #21914]

Culture collection no.

DSM 26265, CCUG 63301, JCM 30766

[Ref.: #88910]

SI-ID 399143

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Lactobacillus apinorum sp. nov., Lactobacillus mellifer sp. nov., Lactobacillus mellis sp. nov., Lactobacillus melliventris sp. nov., Lactobacillus kimbladii sp. nov., Lactobacillus helsingborgensis sp. nov. and Lactobacillus kullabergensis sp. nov., isolated from the honey stomach of the honeybee Apis mellifera.	Olofsson TC, Alsterfjord M, Nilson B, Butler E, Vasquez A	Int J Syst Evol Microbiol	10.1099/ijs.0.059600-0	2014	*
Phylogeny	Lactobacillus panisapium sp. nov., from honeybee Apis cerana bee bread.	Wang C, Huang Y, Li L, Guo J, Wu Z, Deng Y, Dai L, Ma S	Int J Syst Evol Microbiol	10.1099/ijsem.0.002538	2018	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#21914

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 26265

#62968 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 63301
#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68371

Automatically annotated from API 50CH acid .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#88910

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID399143.1 )

* These data were automatically processed and therefore are not curated

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