Rouxiella chamberiensis (DSM 28324, CIP 110714, CCUG 70919)

Name: Rouxiella chamberiensis (DSM 28324, CIP 110714, CCUG 70919)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 130319

Type strain

Strain Designation 130333

Culture col. no. DSM 28324 CIP 110714 CCUG 70919 130333

NCBI tax ID(s) 1513468

Special Collections DSMZ CCUG CIP

Links

Rouxiella chamberiensis 130333 is an aerobe, mesophilic, rod-shaped prokaryote that was isolated from parenteral nutrition bag for premature newborns.

rod-shaped aerobe mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 403855

DSM 28324,CIP 110714,CCUG 70919T

@ref 20215

Last LPSN update 2025-12-16 10:00:58

Domain Pseudomonadati

Phylum Pseudomonadota

Class Gammaproteobacteria

Order Enterobacterales

Family Yersiniaceae

Genus Rouxiella

Species Rouxiella chamberiensis

Full scientific name Rouxiella chamberiensis Le Flèche-Matéos et al. 2015

BacDive ID	Other strains from **Rouxiella chamberiensis** (8)	Type strain
134949	R. chamberiensis 140002, CIP 110766
134950	R. chamberiensis 140004, CIP 110768
134951	R. chamberiensis 140005, CIP 110769
136735	R. chamberiensis 140001, CIP 110765
139033	R. chamberiensis 140003, CIP 110767
157040	R. chamberiensis CCUG 69063
157051	R. chamberiensis CCUG 69149
157059	R. chamberiensis CCUG 69255

Morphology

Cell morphology

@ref	Gram stain	Confidence
125438	negative	99.5
125439	negative	99.4
118223

Colony morphology

63765

Incubation period1 day

Multimedia

@ref:	22156
multimedia content:	DSM_28324.jpg
multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_28324.jpg
intellectual property rights:	© Leibniz-Institut DSMZ
manual_annotation:	1

Culture and growth conditions

Culture medium

@ref	Name	Composition
36240	MEDIUM 72- for trypto casein soja agar	Distilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
22156	TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)	Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
118223	CIP Medium 72

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
22156	positive	growth	28	mesophilic
36240	positive	growth	30	mesophilic
63765	positive	growth	30	mesophilic

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance
63765	aerobe
125439	obligate aerobe

Spore formation

@ref	Spore formation	Confidence
125439		98.1

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68368	27613 ChEBI	amygdalin	+	fermentation	from API 20E
68368	29016 ChEBI	arginine	-	hydrolysis	from API 20E
68368	16947 ChEBI	citrate	+	assimilation	from API 20E
68368	17634 ChEBI	D-glucose	+	fermentation	from API 20E
68368	16899 ChEBI	D-mannitol	+	fermentation	from API 20E
68368	5291 ChEBI	gelatin	-	hydrolysis	from API 20E
68368	30849 ChEBI	L-arabinose	+	fermentation	from API 20E
68368	62345 ChEBI	L-rhamnose	+	fermentation	from API 20E
68368	25094 ChEBI	lysine	-	degradation	from API 20E
68368	17268 ChEBI	myo-inositol	-	fermentation	from API 20E
68368	18257 ChEBI	ornithine	-	degradation	from API 20E
68368	30911 ChEBI	sorbitol	-	fermentation	from API 20E
68368	17992 ChEBI	sucrose	-	fermentation	from API 20E
68368	27897 ChEBI	tryptophan	-	energy source	from API 20E
68368	16199 ChEBI	urea	-	hydrolysis	from API 20E

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68368	16136 ChEBI	hydrogen sulfide		from API 20E
68368	35581 ChEBI	indole		from API 20E

Metabolite tests

@ref	Chebi-ID	Metabolite	Indole test
68368	35581 ChEBI	indole	-	from API 20E

Enzymes

@ref	Value	Activity	Ec
68368	arginine dihydrolase	-	3.5.3.6	from API 20E
68368	beta-galactosidase	+	3.2.1.23	from API 20E
68368	cytochrome oxidase	-	1.9.3.1	from API 20E
68368	gelatinase	-		from API 20E
68368	lysine decarboxylase	-	4.1.1.18	from API 20E
68368	ornithine decarboxylase	-	4.1.1.17	from API 20E
68368	tryptophan deaminase	-	4.1.99.1	from API 20E
68368	urease	-	3.5.1.5	from API 20E

API 20E

@ref	ONPG	ADH (Arg)	LDC (Lys)	ODC	CIT	H2S productionH2S	URE	TDA (Trp)	IND	Acetoin production (Voges Proskauer test)VP	GEL	GLU	MAN	INO	Sor	RHA	SAC	MEL	AMY	ARA	OX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	OF-O	OF-F
22156	+	-	-	-	+	-	-	-	-	+	-	+	+	-	-	+	-	+	+	+	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.
22156	+	-	-	-	+	-	-	-	-	-	-	+	+	-	-	+	-	-	+	+	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2
#Infection	#Medical device
#Infection	#Medical product

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
22156	parenteral nutrition bag for premature newborns	Chambéry	France	FRA	Europe
63765	Parenteral nutrition bags for newborns	Chambery Hospital	France	FRA	Europe
118223	Food, Parenteral food bag	Chambéry	France	FRA	Europe

Taxonmaps

69479

Global distribution of 16S sequence KJ526379 (>99% sequence identity) for Yersiniaceae from Microbeatlas

Aquatic Samples	33
Soil Samples	34
Animal Samples	170
Plant Samples	94
Total Samples	331

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
22156	1	Risk group (German classification)
118223	1	Risk group (French classification)

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM2696747v1 assembly for Rouxiella chamberiensis DSM 28324	complete	GCA_026967475	1513468.9	8118339223	1513468	84.95
66792	ASM95113v1 assembly for Rouxiella chamberiensis 130333	scaffold	GCA_000951135	1513468.4	2639763055	1513468	51.77

Genome browser: GCA_026967475

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
22156	Rouxiella chamberiensis strain 130333 16S ribosomal RNA gene, partial sequence	KJ526379	1463		1513468

GC content

@ref	GC-content (mol%)	Method
22156	53	sequence analysis

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Rouxiella chamberiensis 130333
NCBI: GCA_000951135

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	98.10	no
125439	motility	BacteriaNetⓘ	yes	60.90	no
125439	gram_stain	BacteriaNetⓘ	negative	99.40	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	95.20	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Rouxiella chamberiensis DSM 28324
NCBI: GCA_026967475.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	99.50	no
125438	anaerobic	anaerobicⓘ	no	98.66	yes
125438	aerobic	aerobicⓘ	no	62.97	no
125438	spore-forming	spore-formingⓘ	no	87.50	no
125438	thermophilic	thermophileⓘ	no	99.50	yes
125438	flagellated	motile2+ⓘ	yes	58.45	no

Literature

Title	Authors	Journal	DOI	Year
Complete Genome Sequences of the Type Strains Rouxiella badensis DSM 100043 and R. chamberiensis DSM 28324, Resolved Using Nanopore Long-Read Sequencing.	Paul S, Anderson PJ, Maynard GJ, Dyall-Smith M, Kudinha T.	Microbiol Resour Announc	10.1128/mra.00156-23	2023
Detection of Mycobacterium ulcerans with IS2404 loop-mediated isothermal amplification and a fluorescent reporter probe.	Lee JYH, Porter JL, Globan M, Lavender CJ, Zhang Y, Tanner NA, Hobbs EC, Buultjens AH, Stinear TP.	Appl Environ Microbiol	10.1128/aem.00270-25	2025
Rouxiella chamberiensis gen. nov., sp. nov., a member of the family Enterobacteriaceae isolated from parenteral nutrition bags.	Le Fleche-Mateos A, Levast M, Lomprez F, Arnoux Y, Andonian C, Perraud M, Vincent V, Ar Gouilh M, Thiberge JM, Vandenbogaert M, Diancourt L, Caro V, Burguiere AM, Manuguerra JC	Int J Syst Evol Microbiol	10.1099/ijs.0.000179	2015

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#22156	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 28324
#36240	; Curators of the CIP;
#63765	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 70919
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#68368	Automatically annotated from API 20E .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#118223	Collection of Institut Pasteur ; Curators of the CIP; CIP 110714
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Rouxiella chamberiensis (DSM 28324, CIP 110714, CCUG 70919)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Multimedia

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API 20E

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_026967475

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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