Name: Rouxiella chamberiensis 130333
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 130319

Type strain:

Species: Rouxiella chamberiensis

Strain Designation: 130333

Culture col. no.: DSM 28324, CIP 110714, CCUG 70919

Strain history: CIP <- 2014, A. Le Flèche, Inst. Pasteur, Paris, France: strain 130333 Louisia chamberiensis <- Chambéry Hosp., Chambéry, France

NCBI tax ID(s): 1513468 (species)

SI-ID 403855

DSM 28324, CIP 110714

Other strains from Rouxiella chamberiensis

R. chamberiensis 140002, CIP 110766
R. chamberiensis 140004, CIP 110768
R. chamberiensis 140005, CIP 110769
R. chamberiensis 140001, CIP 110765
R. chamberiensis 140003, CIP 110767
R. chamberiensis CCUG 69063
R. chamberiensis CCUG 69149
R. chamberiensis CCUG 69255

Section

Rouxiella chamberiensis 130333 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from parenteral nutrition bag for premature newborns.

Gram-negative
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Enterobacterales*
	Family	*Yersiniaceae*
	Genus	*Rouxiella*
	Species	**Rouxiella chamberiensis**
	Full Scientific Name (LPSN)	Rouxiella chamberiensis Le Flèche-Matéos et al. 2015

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	negative Confidence in %99.981

[Ref.: #118223]	Cell shape	rod-shaped

[Ref.: #118223]	Motility	no

[Ref.: #63765]

Incubation period

1 day

Multimedia content

[Ref.: #22156]

Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

[Ref.: #36240]

Culture medium

MEDIUM 72- for trypto casein soja agar

[Ref.: #36240]

Culture medium growth

[Ref.: #36240]

Culture medium composition

expand / minimize

[Ref.: #22156]

Culture medium

TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)

[Ref.: #22156]

Culture medium growth

[Ref.: #22156]

Culture medium link

Medium recipe at MediaDive

[Ref.: #22156]

Culture medium composition

expand / minimize

[Ref.: #118223]

Culture medium

CIP Medium 72

[Ref.: #118223]

Culture medium growth

[Ref.: #118223]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #22156]	growth	28 °C
[Ref.: #36240]	growth	30 °C
[Ref.: #63765]	growth	30 °C

[Ref.: #22156]	Temperature range	mesophilic
[Ref.: #36240]	Temperature range	mesophilic
[Ref.: #63765]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #63765]

Oxygen tolerance

aerobe

[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.875

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68368]	amygdalin ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	arginine ChEBI	-	hydrolysis	* from API 20E
[Ref.: #68368]	citrate ChEBI	+	assimilation	* from API 20E
[Ref.: #68368]	D-glucose ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	D-mannitol ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	gelatin ChEBI	-	hydrolysis	* from API 20E
[Ref.: #68368]	L-arabinose ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	L-rhamnose ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	lysine ChEBI	-	degradation	* from API 20E
[Ref.: #68368]	myo-inositol ChEBI	-	fermentation	* from API 20E
[Ref.: #68368]	ornithine ChEBI	-	degradation	* from API 20E
[Ref.: #68368]	sorbitol ChEBI	-	fermentation	* from API 20E
[Ref.: #68368]	sucrose ChEBI	-	fermentation	* from API 20E
[Ref.: #68368]	tryptophan ChEBI	-	energy source	* from API 20E
[Ref.: #68368]	urea ChEBI	-	hydrolysis	* from API 20E
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #68368]		hydrogen sulfide ChEBI	no	* from API 20E
[Ref.: #68368]		indole ChEBI	no	* from API 20E

	Physiological tests	Metabolite	Indole test
[Ref.: #68368]		indole ChEBI	-	* from API 20E

	Enzyme	Enzyme activity	EC number
[Ref.: #68368]	arginine dihydrolase	-	3.5.3.6	* from API 20E
[Ref.: #68368]	beta-galactosidase	+	3.2.1.23	* from API 20E
[Ref.: #68368]	cytochrome oxidase	-	1.9.3.1	* from API 20E
[Ref.: #68368]	gelatinase	-		* from API 20E
[Ref.: #68368]	lysine decarboxylase	-	4.1.1.18	* from API 20E
[Ref.: #68368]	ornithine decarboxylase	-	4.1.1.17	* from API 20E
[Ref.: #68368]	tryptophan deaminase	-	4.1.99.1	* from API 20E
[Ref.: #68368]	urease	-	3.5.1.5	* from API 20E

API® 20E:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	beta-GalactosidaseONPG	Arginine dihydrolaseADH (Arg)	Lysine decarboxylaseLDC (Lys)	Ornithine decarboxylaseODC	Citrate utilizationCIT	H2S productionH2S	Urease/urea hydrolysisURE	Tryptophan deaminaseTDA (Trp)	Indole productionIND	Acetoin production (Voges Proskauer test)VP	Gelatinase/gelatin hydrolysisGEL	Fermentation/oxidation of D-glucoseGLU	Fermentation/oxidation of D-mannitolMAN	Fermentation/oxidation of inositolINO	Fermentation/oxidation of sorbitolSor	Fermentation/oxidation of L-rhamnoseRHA	Fermentation/oxidation of sucroseSAC	Fermentation/oxidation of melibioseMEL	Fermentation/oxidation of amygdalinAMY	Fermentation/oxidation of L-arabinoseARA	Cytochrome oxidaseOX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	Oxidative catabolisation of D-glucoseOF-O	Fermentation of D-glucoseOF-F
[Ref.: #22156]	17863	+	-	-	-	+	-	-	-	-	+	-	+	+	-	-	+	-	+	+	+	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.
[Ref.: #22156]	16690	+	-	-	-	+	-	-	-	-	-	-	+	+	-	-	+	-	-	+	+	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #22156]	Sample type/isolated from	parenteral nutrition bag for premature newborns
[Ref.: #22156]	Geographic location (country and/or sea, region)	Chambéry
[Ref.: #22156]	Country	France
[Ref.: #22156]	Country ISO 3 Code	FRA
[Ref.: #22156]	Continent	Europe

[Ref.: #63765]	Sample type/isolated from	Parenteral nutrition bags for newborns
[Ref.: #63765]	Sampling date	2013-12-01
[Ref.: #63765]	Geographic location (country and/or sea, region)	Chambery Hospital
[Ref.: #63765]	Country	France
[Ref.: #63765]	Country ISO 3 Code	FRA
[Ref.: #63765]	Continent	Europe

[Ref.: #118223]	Sample type/isolated from	Food, Parenteral food bag
[Ref.: #118223]	Geographic location (country and/or sea, region)	Chambéry
[Ref.: #118223]	Country	France
[Ref.: #118223]	Country ISO 3 Code	FRA
[Ref.: #118223]	Continent	Europe
[Ref.: #118223]	Isolation date	2013-12-15
* marker position based on {}

Isolation sources categories

#Infection	#Medical device	-
#Infection	#Medical product	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence KJ526379 (>99% sequence identity) for Yersiniaceae from Microbeatlas

Aquatic Samples	33
Soil Samples	34
Animal Samples	170
Plant Samples	94
Total Samples	331

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #22156]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #118223]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #22156]	Rouxiella chamberiensis strain 130333 16S ribosomal RNA gene, partial sequence	KJ526379	1463	ENA	1513468 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Rouxiella chamberiensis 130333	GCA_000951135	scaffold	GenBank	1513468 tax ID	*
[Ref.: #66792]	Rouxiella chamberiensis 130333	2639763055	draft	JGI IMG/M	1513468 tax ID	*
[Ref.: #66792]	Rouxiella chamberiensis DSM 28324	GCA_026967475	complete	GenBank	1513468 tax ID	*

[Ref.: #22156]

GC-content

53 mol%

sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Rouxiella chamberiensis 130333 NCBI: GCA_000951135.1
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Rouxiella chamberiensis DSM 28324 NCBI: GCA_026967475.1
[Ref.: #69480]	motile	yes	88.174	no
[Ref.: #69480]	flagellated	no	82.101	no
[Ref.: #69480]	gram-positive	no	98.373	no
[Ref.: #69480]	anaerobic	no	99.093	yes
[Ref.: #69480]	aerobic	yes	59.935	yes
[Ref.: #69480]	halophile	no	83.752	no
[Ref.: #69480]	spore-forming	no	96.432	no
[Ref.: #69480]	glucose-ferment	yes	86.183	no
[Ref.: #69480]	thermophile	no	99.357	no
[Ref.: #69480]	glucose-util	yes	95.778	no

Availability in culture collections External links

[Ref.: #22156]

Culture collection no.

DSM 28324, CIP 110714, CCUG 70919

[Ref.: #88852]

SI-ID 403855

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Rouxiella chamberiensis gen. nov., sp. nov., a member of the family Enterobacteriaceae isolated from parenteral nutrition bags.	Le Fleche-Mateos A, Levast M, Lomprez F, Arnoux Y, Andonian C, Perraud M, Vincent V, Ar Gouilh M, Thiberge JM, Vandenbogaert M, Diancourt L, Caro V, Burguiere AM, Manuguerra JC	Int J Syst Evol Microbiol	10.1099/ijs.0.000179	2015	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#22156

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 28324

#36240 ; Curators of the CIP;
#63765 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 70919

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68368

Automatically annotated from API 20E .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#88852

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID403855.1 )

#118223 Collection of Institut Pasteur ; Curators of the CIP; CIP 110714
* These data were automatically processed and therefore are not curated

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Rouxiella chamberiensis 130333

Rouxiella chamberiensis DSM 28324

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