Pseudomonas furukawaii (DSM 10086, NBRC 110670, KF707)

Name: Pseudomonas furukawaii (DSM 10086, NBRC 110670, KF707)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 12966

Type strain

Strain Designation KF707

Culture col. no. DSM 10086 NBRC 110670 KF707

NCBI tax ID(s) 1149133

Special Collections DSMZ Literature strains

Links

Pseudomonas furukawaii KF707 is an aerobe, mesophilic, Gram-negative prokaryote that forms circular colonies and was isolated from soil near a biphenyl manufacturing plant.

Gram-negative motile rod-shaped colony-forming aerobe mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 48745

NBRC 110670,DSM 10086,KCTC 12118,NCIMB 13494,CCUG 75672T

@ref 20215

Last LPSN update 2025-12-16 09:49:59

Domain Pseudomonadati

Phylum Pseudomonadota

Class Gammaproteobacteria

Order Pseudomonadales

Family Pseudomonadaceae

Genus Pseudomonas

Species Pseudomonas furukawaii

Full scientific name Pseudomonas furukawaii Kimura et al. 2018

Synonyms (1)

Metapseudomonas furukawaii

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Confidence
65464	negative	1.5-2.0 µm	0.7-0.9 µm	rod-shaped
125438					92.298
125438	negative				99.5
125439	negative				97.6

Colony morphology

@ref	Colony color	Colony shape
65464	white	circular

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
3795	REACTIVATION WITH LIQUID MEDIUM (DSMZ Medium 535b)	Medium recipe at MediaDive	Name: REACTIVATION WITH LIQUID MEDIUM (DSMZ Medium 535b) Composition: Trypticase soy broth 30.0 g/l Casein peptone 17.0 g/l Agar 15.0 g/l NaCl 5.0 g/l Soy peptone 3.0 g/l K2HPO4 2.5 g/l D(+)-Glucose 2.5 g/l Distilled water
3795	MEDIUM WITH BIPHENYL (DSMZ Medium 457d)	Medium recipe at MediaDive	Name: MEDIUM WITH BIPHENYL (DSMZ Medium 457d) Composition: Na2HPO4 2.44 g/l KH2PO4 1.52 g/l (NH4)2SO4 0.5 g/l Biphenyl 0.25 g/l MgSO4 x 7 H2O 0.2 g/l CaCl2 x 2 H2O 0.05 g/l EDTA 0.005 g/l FeSO4 x 7 H2O 0.002 g/l H3BO3 0.0003 g/l CoCl2 x 6 H2O 0.0002 g/l ZnSO4 x 7 H2O 0.0001 g/l Na2MoO4 x 2 H2O 3e-05 g/l MnCl2 x 4 H2O 3e-05 g/l NiCl2 x 6 H2O 2e-05 g/l CuCl2 x 2 H2O 1e-05 g/l Distilled water
3795	R2A MEDIUM (DSMZ Medium 830)	Medium recipe at MediaDive	Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
3795	positive	growth	30	mesophilic
65464	positive	growth	30-37	mesophilic
65464	negative	growth	42
65464	negative	growth	45

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
65464	aerobe
125438	aerobe	93.392

Spore formation

@ref	Spore formation	Confidence
65464
125439		98.4

Compound production

3795

Compound2,3 dihydroxybiphenyl dioxygenase

Halophily

@ref	Salt	Growth	Tested relation	Concentration
65464	NaCl		growth	8 %

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68369	17128 ChEBI	adipate	-	assimilation	from API 20NE
68369	29016 ChEBI	arginine	+	hydrolysis	from API 20NE
68369	17634 ChEBI	D-glucose	+	assimilation	from API 20NE
68369	17634 ChEBI	D-glucose	-	fermentation	from API 20NE
65464	16899 ChEBI	D-mannitol	-	carbon source
68369	16899 ChEBI	D-mannitol	-	assimilation	from API 20NE
65464	16024 ChEBI	D-mannose	+	assimilation
68369	16024 ChEBI	D-mannose	-	assimilation	from API 20NE
65464	27689 ChEBI	decanoate	-	carbon source
68369	27689 ChEBI	decanoate	+	assimilation	from API 20NE
68369	4853 ChEBI	esculin	-	hydrolysis	from API 20NE
65464	5291 ChEBI	gelatin	+	assimilation
68369	5291 ChEBI	gelatin	-	hydrolysis	from API 20NE
68369	24265 ChEBI	gluconate	+	assimilation	from API 20NE
65464	17234 ChEBI	glucose	-	builds acid from
65464	17234 ChEBI	glucose	+	assimilation
65464	30849 ChEBI	L-arabinose	-	carbon source
68369	30849 ChEBI	L-arabinose	-	assimilation	from API 20NE
65464	25115 ChEBI	malate	-	carbon source
68369	25115 ChEBI	malate	+	assimilation	from API 20NE
65464	17306 ChEBI	maltose	+	assimilation
68369	17306 ChEBI	maltose	-	assimilation	from API 20NE
65464	506227 ChEBI	N-acetylglucosamine	+	assimilation
68369	59640 ChEBI	N-acetylglucosamine	-	assimilation	from API 20NE
65464	17632 ChEBI	nitrate	+	reduction
68369	17632 ChEBI	nitrate	+	reduction	from API 20NE
65464	32032 ChEBI	potassium gluconate	-	carbon source
65464	53258 ChEBI	sodium citrate	+	assimilation
68369	27897 ChEBI	tryptophan	-	energy source	from API 20NE
68369	16199 ChEBI	urea	-	hydrolysis	from API 20NE

Metabolite production

@ref	Chebi-ID	Metabolite	Production
65464	35581 ChEBI	indole
68369	35581 ChEBI	indole		from API 20NE

Metabolite tests

@ref	Chebi-ID	Metabolite	Indole test
68369	35581 ChEBI	indole	-	from API 20NE

Enzymes

@ref	Value	Activity	Ec
65464	arginine dihydrolase	-	3.5.3.6
68369	arginine dihydrolase	+	3.5.3.6	from API 20NE
68369	beta-glucosidase	-	3.2.1.21	from API 20NE
65464	catalase	+	1.11.1.6
65464	cytochrome oxidase	+	1.9.3.1
68369	cytochrome oxidase	+	1.9.3.1	from API 20NE
68369	gelatinase	-		from API 20NE
68369	urease	-	3.5.1.5	from API 20NE

Fatty acid profile

Metadata FA analysis

@ref

65464

Fatty acid	Percentage
C15:0 anteiso	40.13
C16:1ω7c/C16:1ω6c	11.28
C15:0 anteiso	9.01
C16:0	8.16
C17:0 anteiso	4.52
C13:0 iso 3OH	3.52
C12:0	3.32
C16:1ω7c/C16:1ω6c	3.07
C12:0 3OH	2.17
C16:1ω9c	1.84
C18:1ω9c	1.51
C11:0 iso 3OH	1.4
C18:1ω7c	1.18
C13:0 anteiso	0.67
C11:0 anteiso	0.57
C19:0 iso	0.44
C10:0	0.43
C17:0 anteiso	0.32
C13:0 2OH	0.28
C18:0	0.27
C17:0 cyclo	0.15
C17:1ω8c	0.13
C10:0 3OH	0.12
C12:0 iso 3OH	0.11
C10:0 2OH	0.09
C18:0 iso	0.09
C14:1ω5c	0.07
C13:0 anteiso	0.07
C11:0 anteiso	0.04
C12:0 2OH	0.04

API 20NE

@ref	Reduction of nitratesNO3	TRP	GLU_ Ferm	ADH (Arg)	URE	ESC	GEL	PNPG	GLU_ Assim	ARA	MNE	MAN	NAG	MAL	GNT	CAP	ADI	MLT	CIT	PAC	OX
3795	+	-	-	+	-	-	-	-	+	-	-	-	-	-	+	+	-	+	+	+	+

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Industrial	#Plant (Factory)
#Environmental	#Terrestrial	#Soil

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent	Enrichment culture composition	Enrichment culture duration	Enrichment culture temperature
3795	soil near a biphenyl manufacturing plant		Japan	JPN	Asia
65464	from biphenyl-contaminated sites in a biphenyl-manufacturing factory	Kitakyushu	Japan	JPN	Asia	(in g l^-1): 4.3 K2HPO4 ; 3.4 KH2PO4 ; 2.0 (NH4) SO4; 0.16 MgCl2 ; 0.001 MnCl2 4H2O; 0.0006 FeSO4 7H2O; 0.026 CaCl2 2H2O; and 0.02Na2MoO4 2H2O. Substrate (biphenyl or succinate) was added at a concentration of 1g l^-1	7 days	30

Interaction and safety

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM235547v1 assembly for Metapseudomonas furukawaii KF707	complete	GCA_002355475	1149133.19	2814123110	1149133	93.48
66792	KF707_a1 assembly for Metapseudomonas furukawaii KF707	scaffold	GCA_000262065	1149133.6	2531839224	1149133	23.7

16S sequences

@ref	Description	Accession	Database
65464	Pseudomonas pseudoalcaligenes gene for 16S rRNA, partial sequence, strain: KF707	AB109887

GC content

@ref	GC-content (mol%)	Method
65464	60.5	sequence analysis

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Pseudomonas pseudoalcaligenes KF707 = NBRC 110670
PATRIC: 1149133.19

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	facultative aerobe	63.20	no
125439	gram_stain	BacteriaNetⓘ	negative	97.60	no
125439	motility	BacteriaNetⓘ	yes	89.70	no
125439	spore_formation	BacteriaNetⓘ	no	98.40	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Pseudomonas furukawaii KF707
NCBI: GCA_002355475.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	99.50	yes
125438	anaerobic	anaerobicⓘ	no	98.16	yes
125438	aerobic	aerobicⓘ	yes	93.39	yes
125438	spore-forming	spore-formingⓘ	no	87.97	yes
125438	thermophilic	thermophileⓘ	no	99.49	no
125438	flagellated	motile2+ⓘ	yes	92.30	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Validation List no. 219. Valid publication of new names and new combinations effectively published outside the IJSEM.	Oren A, Goker M.	Int J Syst Evol Microbiol	10.1099/ijsem.0.006452	2024
Phylogeny	Phylogenomics studies and molecular markers reliably demarcate genus Pseudomonas sensu stricto and twelve other Pseudomonadaceae species clades representing novel and emended genera.	Rudra B, Gupta RS.	Front Microbiol	10.3389/fmicb.2023.1273665	2023
	Predominant Biphenyl Dioxygenase From Legacy Polychlorinated Biphenyl (PCB)-Contaminated Soil Is a Part of Unusual Gene Cluster and Transforms Flavone and Flavanone.	Suman J, Strejcek M, Zubrova A, Capek J, Wald J, Michalikova K, Hradilova M, Sredlova K, Semerad J, Cajthaml T, Uhlik O	Front Microbiol	10.3389/fmicb.2021.644708	2021
Enzymology	A poplar short-chain dehydrogenase reductase plays a potential key role in biphenyl detoxification.	Contreras A, Merino I, Alvarez E, Bolonio D, Ortiz JE, Onate-Sanchez L, Gomez L	Proc Natl Acad Sci U S A	10.1073/pnas.2103378118	2021
Metabolism	Biphenyl/PCB Degrading bph Genes of Ten Bacterial Strains Isolated from Biphenyl-Contaminated Soil in Kitakyushu, Japan: Comparative and Dynamic Features as Integrative Conjugative Elements (ICEs).	Hirose J, Fujihara H, Watanabe T, Kimura N, Suenaga H, Futagami T, Goto M, Suyama A, Furukawa K	Genes (Basel)	10.3390/genes10050404	2019
Phylogeny	Pseudomonas lalkuanensis sp. nov., isolated from a bacterial consortia of contaminated soil enriched for the remediation of e-waste.	Thorat V, Kirdat K, Tiwarekar B, DaCosta E, Debbarma P, Shouche Y, Sathe S, Goel R, Lodha T, Yadav A	Int J Syst Evol Microbiol	10.1099/ijsem.0.004559	2020
Phylogeny	Pseudomonas mangiferae sp. nov., isolated from bark of mango tree in Thailand.	Anurat P, Duangmal K, Srisuk N	Int J Syst Evol Microbiol	10.1099/ijsem.0.003657	2019
Phylogeny	Pseudomonas urumqiensis sp. nov., isolated from rhizosphere soil of Alhagi sparsifolia.	Zou Y, He S, Sun Y, Zhang X, Liu Y, Cheng Q	Int J Syst Evol Microbiol	10.1099/ijsem.0.003390	2019
Phylogeny	Some facts about the respiratory enzymes of Pseudomonas pseudoalcaligenes KF707 recently renamed as Pseudomonas furukawaii sp. nov., type strain KF707.	Fedi S, Cappelletti M, Sandri F, Turner RJ, Zannoni D	Int J Syst Evol Microbiol	10.1099/ijsem.0.002923	2018
Phylogeny	Pseudomonas furukawaii sp. nov., a polychlorinated biphenyl-degrading bacterium isolated from biphenyl-contaminated soil in Japan.	Kimura N, Watanabe T, Suenaga H, Fujihara H, Futagami T, Goto M, Hanada S, Hirose J	Int J Syst Evol Microbiol	10.1099/ijsem.0.002670	2018

References

#3795	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 10086
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#65464	Nobutada Kimura, Takahito Watanabe, Hikaru Suenaga, Hidehiko Fujihara, Taiki Futagami, Masatoshi Goto, Satoshi Hanada, Jun Hirose: Pseudomonas furukawaii sp. nov., a polychlorinated biphenyl-degrading bacterium isolated from biphenyl-contaminated soil in Japan. IJSEM 68: 1429 - 1435 2018 ( DOI 10.1099/ijsem.0.002670 , PubMed 29595413 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#68369	Automatically annotated from API 20NE .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

Rate our new design

Content

About us

Pseudomonas furukawaii (DSM 10086, NBRC 110670, KF707)

Name and taxonomic classification