Name: Methylophaga lonarensis MPL
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 11908

Type strain:

Species: Methylophaga lonarensis

Strain Designation: MPL

Culture col. no.: DSM 24656, MCC 1002, VKM B-2684

Strain history: <- C. P. Antony, NCCS; MPL

NCBI tax ID(s): 999151 (species)

Section

Methylophaga lonarensis MPL is an aerobe, Gram-negative, motile bacterium that was isolated from soda lake sediments of a meteorite impact lake.

Gram-negative
motile
rod-shaped
aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Beggiatoales*
	Family	*Piscirickettsiaceae*
	Genus	*Methylophaga*
	Species	**Methylophaga lonarensis**
	Full Scientific Name (LPSN)	Methylophaga lonarensis Antony et al. 2012

Information on morphological and physiological properties Morphology

[Ref.: #30396]	Gram stain	negative

[Ref.: #30396]	Cell length	1.6 µm

[Ref.: #30396]	Cell width	0.2 µm

[Ref.: #30396]	Cell shape	rod-shaped

[Ref.: #30396]	Motility	yes

[Ref.: #17721]

Incubation period

2-3 days

[Ref.: #30396]

Pigment production

yes

Information on culture and growth conditions Culture and growth conditions

	Temperatures	Kind of temperature		Temperature
[Ref.: #30396]			growth	20-37 °C
[Ref.: #30396]			optimum	29 °C

[Ref.: #30396]

Temperature range

mesophilic

	pH	Kind of pH		pH
[Ref.: #30396]			optimum	9.5
[Ref.: #30396]			growth	07-10

Information on physiology and metabolism Physiology and metabolism

[Ref.: #30396]

Oxygen tolerance

aerobe

[Ref.: #30396]

Ability of spore formation

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #30396]		NaCl		growth	0.05-10	%
[Ref.: #30396]		NaCl		optimum	1.50	%

	Metabolite utilization	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #30396]		nitrate ChEBI	+	reduction

	Enzyme	Enzyme activity	EC number
[Ref.: #30396]	catalase	+	1.11.1.6
[Ref.: #30396]	cytochrome oxidase	+	1.9.3.1
[Ref.: #30396]	urease	+	3.5.1.5

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #17721]	Sample type/isolated from	soda lake sediments of a meteorite impact lake
[Ref.: #17721]	Geographic location (country and/or sea, region)	Maharashtra state, Buldhana district, Lonar Lake
[Ref.: #17721]	Country	India
[Ref.: #17721]	Country ISO 3 Code	IND
[Ref.: #17721]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Lake (large)
#Environmental	#Aquatic	#Sediment
#Condition	#Alkaline	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence JF330773 (>99% sequence identity) for Methylophaga lonarensis subclade from Microbeatlas

Aquatic Samples	723
Soil Samples	192
Animal Samples	366
Plant Samples	36
Total Samples	1317

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #17721]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #17721]	Methylophaga lonarensis strain MPL 16S ribosomal RNA gene, partial sequence	JF330773	1491	ENA	1286106 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Methylophaga lonarensis MPL	GCA_000349205	contig	GenBank	1286106 tax ID	*
[Ref.: #66792]	Methylophaga lonarensis MPL	1286106.3	wgs	PATRIC	1286106 tax ID	*
[Ref.: #66792]	Methylophaga lonarensis MPL	2527291626	draft	JGI IMG/M	1286106 tax ID	*

[Ref.: #17721]	GC-content	50.0 mol%	thermal denaturation, midpoint method (Tm)
[Ref.: #30396]	GC-content	50 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Methylophaga lonarensis MPL PATRIC: 1286106.3
[Ref.: #69480]	motile	yes	84.689	no
[Ref.: #69480]	gram-positive	no	98.87	yes
[Ref.: #69480]	anaerobic	no	96.937	yes
[Ref.: #69480]	aerobic	yes	76.159	yes
[Ref.: #69480]	halophile	no	86.236	yes
[Ref.: #69480]	spore-forming	no	97.659	yes
[Ref.: #69480]	thermophile	no	98.392	yes
[Ref.: #69480]	glucose-util	yes	58.471	no
[Ref.: #69480]	flagellated	yes	68.094	no
[Ref.: #69480]	glucose-ferment	no	85.412	no

Availability in culture collections External links

[Ref.: #17721]

Culture collection no.

DSM 24656, MCC 1002, VKM B-2684

Literature:

Topic	Title	Authors	Journal	DOI	Year
Genetics	Draft Genome Sequence of Methylophaga lonarensis MPLT, a Haloalkaliphilic (Non-Methane-Utilizing) Methylotroph.	Shetty SA, Marathe NP, Munot H, Antony CP, Dhotre DP, Murrell JC, Shouche YS	Genome Announc	10.1128/genomeA.00202-13	2013	*
Phylogeny	Methylophaga lonarensis sp. nov., a moderately haloalkaliphilic methylotroph isolated from the soda lake sediments of a meteorite impact crater.	Antony CP, Doronina NV, Boden R, Trotsenko YA, Shouche YS, Murrell JC	Int J Syst Evol Microbiol	10.1099/ijs.0.035089-0	2011	*

References

#17721

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 24656

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#30396

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26733 (see below)

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#26733

IJSEM 1613 2012 ( DOI 10.1099/ijs.0.035089-0 , PubMed 21890731 )

* These data were automatically processed and therefore are not curated

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