Desulfotomaculum nigrificans CO-1-SRB is an anaerobe, spore-forming, thermophilic prokaryote that was isolated from sludge from an anaerobic bioreactor treating paper mill wastewater.
spore-forming Gram-positive motile rod-shaped anaerobe thermophilic genome sequence 16S sequence| @ref 20215 |
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Last LPSN update
2025-12-16 09:49:42 |
| Domain Bacillati |
| Phylum Bacillota |
| Class Clostridia |
| Order Eubacteriales |
| Family Peptococcaceae |
| Genus Desulfotomaculum |
| Species Desulfotomaculum nigrificans |
| Full scientific name Desulfotomaculum nigrificans (Werkman and Weaver 1927) Campbell and Postgate 1965 (Approved Lists 1980) |
| Synonyms (2) |
| @ref | Name | Growth | Medium link | Composition | |
|---|---|---|---|---|---|
| 5596 | DESULFOBULBUS SP. MEDIUM (FRESHWATER) (DSMZ Medium 194) | Medium recipe at MediaDive  | Name: DESULFOBULBUS SP. MEDIUM (FRESHWATER) (DSMZ Medium 194; with strain-specific modifications) Composition: Na2SO4 2.99103 g/l Na-pyruvate 2.19342 g/l Na2CO3 1.49551 g/l NaCl 0.997009 g/l Yeast extract 0.5 g/l KCl 0.498504 g/l Na2S x 9 H2O 0.398804 g/l MgCl2 x 6 H2O 0.398804 g/l NH4Cl 0.299103 g/l KH2PO4 0.199402 g/l CaCl2 x 2 H2O 0.149551 g/l HCl 0.00249252 g/l FeCl2 x 4 H2O 0.00149551 g/l NaOH 0.000498504 g/l Sodium resazurin 0.000498504 g/l CoCl2 x 6 H2O 0.000189432 g/l MnCl2 x 4 H2O 9.97009e-05 g/l Pyridoxine hydrochloride 9.97009e-05 g/l ZnCl2 6.97906e-05 g/l (DL)-alpha-Lipoic acid 4.98504e-05 g/l Nicotinic acid 4.98504e-05 g/l p-Aminobenzoic acid 4.98504e-05 g/l Riboflavin 4.98504e-05 g/l Calcium D-(+)-pantothenate 4.98504e-05 g/l Thiamine HCl 4.98504e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l NiCl2 x 6 H2O 2.39282e-05 g/l Biotin 1.99402e-05 g/l Folic acid 1.99402e-05 g/l H3BO3 5.98205e-06 g/l Na2WO4 x 2 H2O 3.98804e-06 g/l Na2SeO3 x 5 H2O 2.99103e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l Vitamin B12 9.97009e-07 g/l Distilled water |
| Cat1 | Cat2 | |
|---|---|---|
| #Engineered | #Bioreactor | |
| #Engineered | #Waste | |
| #Environmental | #Terrestrial | |
| #Condition | #Anoxic (anaerobic) |
| @ref | Sample type | Geographic location | Country | Country ISO 3 Code | Continent | |
|---|---|---|---|---|---|---|
| 5596 | sludge from an anaerobic bioreactor treating paper mill wastewater | Eerbeek, Industriewater Eerbeek BV | Netherlands | NLD | Europe |
Global distribution of 16S sequence AY961415 (>99% sequence identity) for Desulfotomaculum nigrificans subclade from Microbeatlas 
 Aquatic Samples |
688 |
 Soil Samples |
516 |
 Animal Samples |
5582 |
 Plant Samples |
145 |
| Total Samples | 6931 |
| @ref | Description | Assembly level | INSDC accession | BV-BRC accession | IMG accession | NCBI tax ID | Score | |
|---|---|---|---|---|---|---|---|---|
| 66792 | ASM21443v1 assembly for Desulfotomaculum nigrificans CO-1-SRB | complete | GCA_000214435   | 868595.4  | 2505679104  | 868595 | 97.61 |  |
| 5596 | GC-content (mol%)45.6 |
| Title | Authors | Journal | DOI | Year | |
|---|---|---|---|---|---|
| Genome analyses of the carboxydotrophic sulfate-reducers Desulfotomaculum nigrificans and Desulfotomaculum carboxydivorans and reclassification of Desulfotomaculum caboxydivorans as a later synonym of Desulfotomaculum nigrificans. | Visser M, Parshina SN, Alves JI, Sousa DZ, Pereira IA, Muyzer G, Kuever J, Lebedinsky AV, Koehorst JJ, Worm P, Plugge CM, Schaap PJ, Goodwin LA, Lapidus A, Kyrpides NC, Detter JC, Woyke T, Chain P, Davenport KW, Spring S, Rohde M, Klenk HP, Stams AJ. | Stand Genomic Sci | 10.4056/sigs.4718645 | 2014 | |
| Evaluation of a fluidised catalytic cracking co-processing method for the production of renewable fuels using Category 3 animal fat and used cooking oils. | EFSA Panel on Biological Hazards (BIOHAZ), Allende A, Bortolaia V, Bover-Cid S, Dohmen W, Guillier L, Herman L, Jacxsens L, Nauta M, Mughini-Gras L, Ottoson J, Peixe L, Perez-Rodriguez F, Skandamis P, Suffredini E, De Cesare A, Escamez PF, Griffin J, Kryemadhi K, Ortiz-Pelaez A, Alvarez-Ordonez A. | EFSA J | 10.2903/j.efsa.2025.9337 | 2025 | |
| Screening of a New Kosakonia Species for Polyethylene Biodegradation. | Cho JH, Yun SD, Kim HW, Seo MJ, Sung BH, Yeom SJ. | J Microbiol Biotechnol | 10.4014/jmb.2411.11058 | 2025 | |
| Review of Desulfotomaculum species and proposal of the genera Desulfallas gen. nov., Desulfofundulus gen. nov., Desulfofarcimen gen. nov. and Desulfohalotomaculum gen. nov. | Watanabe M, Kojima H, Fukui M. | Int J Syst Evol Microbiol | 10.1099/ijsem.0.002915 | 2018 | |
| Biocorrosion of dental alloys due to Desulfotomaculum nigrificans bacteria. | Mystkowska J. | Acta Bioeng Biomech | 2016 | | |
| Biocorrosion of 316LV steel used in oral cavity due to Desulfotomaculum nigrificans bacteria. | Mystkowska J, Ferreira JA, Leszczynska K, Chmielewska S, Dabrowski JR, Wiecinski P, Kurzydlowski KJ. | J Biomed Mater Res B Appl Biomater | 10.1002/jbm.b.33518 | 2017 | |
| Study of Anticorrosion and Antifouling Properties of a Cu-Doped TiO2 Coating Fabricated via Micro-Arc Oxidation. | Hu P, Zhu L, Tian C, Xu G, Zhang X, Cai G. | Materials (Basel) | 10.3390/ma17010217 | 2023 | |
| Microbiologically Influenced Corrosion of Carbon Steel Beneath a Deposit in CO2-Saturated Formation Water Containing Desulfotomaculum nigrificans. | Liu H, Meng G, Li W, Gu T, Liu H. | Front Microbiol | 10.3389/fmicb.2019.01298 | 2019 | |
| Expansion segments in bacterial and archaeal 5S ribosomal RNAs. | Stepanov VG, Fox GE. | RNA | 10.1261/rna.077123.120 | 2021 | |
| Recoding of the selenocysteine UGA codon by cysteine in the presence of a non-canonical tRNACys and elongation factor SelB. | Vargas-Rodriguez O, Englert M, Merkuryev A, Mukai T, Soll D. | RNA Biol | 10.1080/15476286.2018.1474074 | 2018 | |
| Dramatic loss of microbial viability in bentonite exposed to heat and gamma radiation: implications for deep geological repository. | Bartak D, Sachlova S, Kaspar V, Riha J, Dobrev D, Vecernik P, Hlavackova V, Matulova M, Cerna K. | World J Microbiol Biotechnol | 10.1007/s11274-024-04069-w | 2024 | |
| Utility of Eucalyptus tereticornis (Smith) bark and Desulfotomaculum nigrificans for the remediation of acid mine drainage. | Chockalingam E, Subramanian S. | Bioresour Technol | 10.1016/j.biortech.2008.07.004 | 2009 | |
| Effect of deposit chemistry on microbial community structure and activity: Implications for under-deposit microbial corrosion. | Diaz-Mateus MA, Salgar-Chaparro SJ, Machuca LL, Farhat H. | Front Microbiol | 10.3389/fmicb.2023.1089649 | 2023 | |
| Bioremediation of zinc using Desulfotomaculum nigrificans: bioprecipitation and characterization studies. | Radhika V, Subramanian S, Natarajan KA. | Water Res | 10.1016/j.watres.2006.06.013 | 2006 | |
| Preparation of nickel-iron hydroxides by microorganism corrosion for efficient oxygen evolution. | Yang H, Gong L, Wang H, Dong C, Wang J, Qi K, Liu H, Guo X, Xia BY. | Nat Commun | 10.1038/s41467-020-18891-x | 2020 | |
| Rice husk filtrate as a nutrient medium for the growth of Desulfotomaculum nigrificans: characterisation and sulfate reduction studies. | Chockalingam E, Sivapriya K, Subramanian S, Chandrasekaran S. | Bioresour Technol | 10.1016/j.biortech.2005.01.032 | 2005 | |
| Studies on removal of metal ions and sulphate reduction using rice husk and Desulfotomaculum nigrificans with reference to remediation of acid mine drainage. | Chockalingam E, Subramanian S. | Chemosphere | 10.1016/j.chemosphere.2005.05.013 | 2006 | |
| ATP sulfurylase activity of sulfate-reducing bacteria from various ecotopes. | Abdulina D, Kovac J, Iutynska G, Kushkevych I. | 3 Biotech | 10.1007/s13205-019-2041-9 | 2020 | |
| Recent advances in emerging integrated anticorrosion and antifouling nanomaterial-based coating solutions. | Thomas P, Sahoo BN, Thomas PJ, Greve MM. | Environ Sci Pollut Res Int | 10.1007/s11356-024-33825-6 | 2024 | |
| Enrichment of syngas-converting communities from a multi-orifice baffled bioreactor. | Arantes AL, Alves JI, Stams AJM, Alves MM, Sousa DZ. | Microb Biotechnol | 10.1111/1751-7915.12864 | 2018 | |
| Positive Effect of Lactobacillus acidophilus EG004 on Cognitive Ability of Healthy Mice by Fecal Microbiome Analysis Using Full-Length 16S-23S rRNA Metagenome Sequencing. | Jeon S, Kim H, Kim J, Seol D, Jo J, Choi Y, Cho S, Kim H. | Microbiol Spectr | 10.1128/spectrum.01815-21 | 2022 | |
| A d-Phenylalanine-Benzoxazole Derivative Reveals the Role of the Essential Enzyme Rv3603c in the Pantothenate Biosynthetic Pathway of Mycobacterium tuberculosis. | Pepi MJ, Chacko S, Marqus GM, Singh V, Wang Z, Planck K, Cullinane RT, Meka PN, Gollapalli DR, Ioerger TR, Rhee KY, Cuny GD, Boshoff HIM, Hedstrom L. | ACS Infect Dis | 10.1021/acsinfecdis.1c00461 | 2022 | |
| Control of bacterial spores. | Brown KL. | Br Med Bull | 10.1258/0007142001902860 | 2000 | |
| Corrosion Behavior of AISI 1045 Steel in Seawater in the Presence of Flavobacterium sp. | Wu J, Zhang W, Chai K, Yu A. | Front Microbiol | 10.3389/fmicb.2020.00303 | 2020 | |
| Fractionation of sulfur isotopes during dissimilatory reduction of sulfate by a thermophilic gram-negative bacterium at 60 degrees C | Bottcher ME, Sievert SM, Kuever J. | Arch Microbiol | 10.1007/s002030050749 | 1999 | |
| Corrosion of dental alloys in artificial saliva with Streptococcus mutans. | Lu C, Zheng Y, Zhong Q. | PLoS One | 10.1371/journal.pone.0174440 | 2017 | |
| Phylogenetic analysis of the genus Desulfotomaculum: evidence for the misclassification of Desulfotomaculum guttoideum and description of Desulfotomaculum orientis as Desulfosporosinus orientis gen. nov., comb. nov. | Stackebrandt E, Sproer C, Rainey FA, Burghardt J, Pauker O, Hippe H. | Int J Syst Bacteriol | 10.1099/00207713-47-4-1134 | 1997 | |
| Structural and chemical characterization of S-layers of selected strains of Bacillus stearothermophilus and Desulfotomaculum nigrificans. | Sleytr UB, Sara M, Kupcu Z, Messner P. | Arch Microbiol | 10.1007/bf00690152 | 1986 | |
| Reduction of bisulfite by the trithionate pathway by cell extracts from Desulfotomaculum nigrificans. | Akagi JM. | Biochem Biophys Res Commun | 10.1016/0006-291x(83)91232-9 | 1983 | |
| Diversity and distribution of thermophilic hydrogenogenic carboxydotrophs revealed by microbial community analysis in sediments from multiple hydrothermal environments in Japan. | Omae K, Fukuyama Y, Yasuda H, Mise K, Yoshida T, Sako Y. | Arch Microbiol | 10.1007/s00203-019-01661-9 | 2019 | |
| Nested PCR-denaturing gradient gel electrophoresis approach to determine the diversity of sulfate-reducing bacteria in complex microbial communities. | Dar SA, Kuenen JG, Muyzer G. | Appl Environ Microbiol | 10.1128/aem.71.5.2325-2330.2005 | 2005 | |
| Thiosulfate disproportionation by Desulfotomaculum thermobenzoicum. | Jackson BE, McInerney MJ. | Appl Environ Microbiol | 10.1128/aem.66.8.3650-3653.2000 | 2000 | |
| Characterization of Germination of Desulfotomaculum nigrificans Spores 1. | Donnelly LS, Busta FF. | J Food Prot | 10.4315/0362-028x-45.8.721 | 1982 | |
| [Submicroscopic organization and spore formation of Desulfotomaculum nigrificans]. | Nazina TN, Pivovarova TA. | Mikrobiologiia | 1979 | | |
| Thermophilic Organisms in Food Spoilage: Sulfide Spoilage Anaerobes | Speck RV. | J Food Prot | 1981 | | |
| Thermophilic Organisms in Food Spoilage: Sulfide Spoilage Anaerobes. | Speck RV. | J Food Prot | 10.4315/0362-028x-44.2.149 | 1981 | |
| Monoclonal antibodies for use in detection of Bacillus and Clostridium spores. | Quinlan JJ, Foegeding PM. | Appl Environ Microbiol | 10.1128/aem.63.2.482-487.1997 | 1997 | |
| Growth of sulfate-reducing bacteria with sulfur as electron acceptor. | Biebl H, Pfennig. | Arch Microbiol | 10.1007/bf00446664 | 1977 | |
| Heat resistance of Desulfotomaculum nigrificans spores in soy protein infant formula preparations. | Donnelly LS, Busta FF. | Appl Environ Microbiol | 10.1128/aem.40.4.721-725.1980 | 1980 | |
| Biochemical and immunological study of cell envelope proteins in sulfate-reducing bacteria. | Norqvist A, Roffey R. | Appl Environ Microbiol | 10.1128/aem.50.1.31-37.1985 | 1985 | |
| Deoxyribonucleic Acid Base Composition of Desulfotomaculum nigrificans. | Saunders GF, Campbell LL. | J Bacteriol | 10.1128/jb.92.2.515-515.1966 | 1966 | |
| Observations on the rhodanese activity of Desulfotomaculum nigrificans. | Burton CP, Akagi JM. | J Bacteriol | 10.1128/jb.107.1.375-376.1971 | 1971 | |
| Isolation of tocopherol from Desulfotomaculum nigrificans. | Cinquina CL. | J Bacteriol | 10.1128/jb.95.6.2436-2438.1968 | 1968 | |
| Observations on the bisulfite reductase (P582) isolated from Desulfotomaculum nigrificans. | Akagi JM, Chan M, Adams V. | J Bacteriol | 10.1128/jb.120.1.240-244.1974 | 1974 | |
| Isolation of a bisulfite reductase activity from Desulfotomaculum nigrificans and its identification as the carbon monoxide-binding pigment P582. | Akagi JM, Adams V. | J Bacteriol | 10.1128/jb.116.1.392-396.1973 | 1973 | |
| Comparative bioenergetics of sulfate reduction in Desulfovibrio and Desulfotomaculum spp. | Liu CL, Peck HD. | J Bacteriol | 10.1128/jb.145.2.966-973.1981 | 1981 | |
| Suspension array analysis of 16S rRNA from Fe- and SO(4)2- reducing bacteria in uranium-contaminated sediments undergoing bioremediation. | Chandler DP, Jarrell AE, Roden ER, Golova J, Chernov B, Schipma MJ, Peacock AD, Long PE. | Appl Environ Microbiol | 10.1128/aem.02858-05 | 2006 | |
| Evidence for aceticlastic methanogenesis in the presence of sulfate in a gas condensate-contaminated aquifer. | Struchtemeyer CG, Elshahed MS, Duncan KE, McInerney MJ. | Appl Environ Microbiol | 10.1128/aem.71.9.5348-5353.2005 | 2005 | |
| Homology of ribosomal ribonucleic acid of Desulfovibrio species with Desulfovibrio vulgaris. | Pace B, Campbell LL. | J Bacteriol | 10.1128/jb.106.3.717-719.1971 | 1971 | |
| Degradation of Glucose by Proliferating Cells of Desulfotomaculum nigrificans. | Akagi JM, Jackson G. | Appl Microbiol | 10.1128/am.15.6.1427-1430.1967 | 1967 | |
| Thiosulfate reductase isolated from Desulfotomaculum nigrificans. | Nakatsukasa W, Akagi JM. | J Bacteriol | 10.1128/jb.98.2.429-433.1969 | 1969 | |
| Identification and isolation of anaerobic, syntrophic phthalate isomer-degrading microbes from methanogenic sludges treating wastewater from terephthalate manufacturing. | Qiu YL, Sekiguchi Y, Imachi H, Kamagata Y, Tseng IC, Cheng SS, Ohashi A, Harada H. | Appl Environ Microbiol | 10.1128/aem.70.3.1617-1626.2004 | 2004 | |
| Cultivation and in situ detection of a thermophilic bacterium capable of oxidizing propionate in syntrophic association with hydrogenotrophic methanogens in a thermophilic methanogenic granular sludge. | Imachi H, Sekiguchi Y, Kamagata Y, Ohashi A, Harada H. | Appl Environ Microbiol | 10.1128/aem.66.8.3608-3615.2000 | 2000 | |
| Carbon monoxide-reacting pigment from Desulfotomaculum nigrificans and its possible relevance to sulfite reduction. | Trudinger PA. | J Bacteriol | 10.1128/jb.104.1.158-170.1970 | 1970 | |
| Filter materials for metal removal from mine drainage--a review. | Westholm LJ, Repo E, Sillanpaa M. | Environ Sci Pollut Res Int | 10.1007/s11356-014-2903-y | 2014 | |
| Effect of Sulfate on Carbon Monoxide Conversion by a Thermophilic Syngas-Fermenting Culture Dominated by a Desulfofundulus Species. | Alves JI, Visser M, Arantes AL, Nijsse B, Plugge CM, Alves MM, Stams AJM, Sousa DZ | Front Microbiol | 10.3389/fmicb.2020.588468 | 2020 | |
| Engineering of vitamin prototrophy in Clostridium ljungdahlii and Clostridium autoethanogenum. | Annan FJ, Al-Sinawi B, Humphreys CM, Norman R, Winzer K, Kopke M, Simpson SD, Minton NP, Henstra AM | Appl Microbiol Biotechnol | 10.1007/s00253-019-09763-6 | 2019 | |
| Physiological and comparative proteomic characterization of Desulfolithobacter dissulfuricans gen. nov., sp. nov., a novel mesophilic, sulfur-disproportionating chemolithoautotroph from a deep-sea hydrothermal vent. | Hashimoto Y, Shimamura S, Tame A, Sawayama S, Miyazaki J, Takai K, Nakagawa S. | Front Microbiol | 10.3389/fmicb.2022.1042116 | 2022 | |
| Desulfotomaculum carboxydivorans sp. nov., a novel sulfate-reducing bacterium capable of growth at 100% CO. | Parshina SN, Sipma J, Nakashimada Y, Henstra AM, Smidt H, Lysenko AM, Lens PNL, Lettinga G, Stams AJM | Int J Syst Evol Microbiol | 10.1099/ijs.0.63780-0 | 2005 | |
How to citeIf you want to cite this particular strain cite the following doi:
https://doi.org/10.13145/bacdive11828.20251217.10
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BacDive in 2025: the core database for prokaryotic strain data
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