Name: Paenibacillus purispatii ES_MS17
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 11653

Type strain:

Species: Paenibacillus purispatii

Strain Designation: ES_MS17, ES-MS17

Culture col. no.: DSM 22991, CIP 110057

Strain history: CIP <- 2009 <- R. Pukall, DSMZ, Germany <- C. Moisse-Eichinger: strain ES-MS17

NCBI tax ID(s): 556177 (species)

SI-ID 376136

CIP 110057, DSM 22991

Section

Paenibacillus purispatii ES_MS17 is a facultative anaerobe, mesophilic, Gram-positive bacterium that was isolated from spacecraft assembly clean room at the European Space Research and Technology Centre .

Gram-positive
motile
rod-shaped
facultative anaerobe
mesophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Paenibacillaceae*
	Genus	*Paenibacillus*
	Species	**Paenibacillus purispatii**
	Full Scientific Name (LPSN)	Paenibacillus purispatii Behrendt et al. 2011

Information on morphological and physiological properties Morphology

[Ref.: #122063]	Gram stain	positive

[Ref.: #122063]	Cell shape	rod-shaped

[Ref.: #122063]	Motility	yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #16640]

Culture medium

CASO AGAR (MERCK 105458) (DSMZ Medium 220)

[Ref.: #16640]

Culture medium growth

[Ref.: #16640]

Culture medium link

Medium recipe at MediaDive

[Ref.: #16640]

Culture medium composition

expand / minimize

[Ref.: #36040]

Culture medium

MEDIUM 72- for trypto casein soja agar

[Ref.: #36040]

Culture medium growth

[Ref.: #36040]

Culture medium composition

expand / minimize

[Ref.: #122063]

Culture medium

CIP Medium 72

[Ref.: #122063]

Culture medium growth

[Ref.: #122063]

Culture medium link

Medium recipe provided by DSMZ

	Temperatures	Kind of temperature		Temperature
[Ref.: #16640]			growth	30 °C
[Ref.: #36040]			growth	30 °C

[Ref.: #16640]	Temperature range	mesophilic
[Ref.: #36040]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #122063]

Oxygen tolerance

facultative anaerobe

	Metabolite utilization	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #122063]		nitrate ChEBI	-	reduction
[Ref.: #122063]		nitrite ChEBI	-	reduction

	Metabolite production	Metabolite	Production
[Ref.: #122063]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	+	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	+	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	+	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #122063]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #122063]	oxidase	+
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #122063]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #122063]	12331	-	-	+	+	-	+	-	-	-	+	-	+	-	+	-	+	+	-	-	+

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #16640]	Sample type/isolated from	spacecraft assembly clean room at the European Space Research and Technology Centre (ESTEC)
[Ref.: #16640]	Geographic location (country and/or sea, region)	Noordwijk
[Ref.: #16640]	Country	Netherlands
[Ref.: #16640]	Country ISO 3 Code	NLD
[Ref.: #16640]	Continent	Europe

[Ref.: #122063]	Sample type/isolated from	Room, clean
[Ref.: #122063]	Geographic location (country and/or sea, region)	Noorwijk
[Ref.: #122063]	Country	Netherlands
[Ref.: #122063]	Country ISO 3 Code	NLD
[Ref.: #122063]	Continent	Europe
[Ref.: #122063]	Isolation date	2007
* marker position based on {}

Isolation sources categories

#Engineered

#Built environment

#Clean room

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence EU888513 (>99% sequence identity) for Paenibacillus from Microbeatlas

Aquatic Samples
Soil Samples	4
Animal Samples	1
Plant Samples	5
Total Samples	10

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #16640]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #122063]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #16640]	Paenibacillus purispatii strain ES_MS17 16S ribosomal RNA gene, partial sequence	EU888513	1318	ENA	556177 tax ID

Availability in culture collections External links

[Ref.: #16640]

Culture collection no.

DSM 22991, CIP 110057

[Ref.: #80877]

SI-ID 376136

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Characterization of heterotrophic nitrifying bacteria with respiratory ammonification and denitrification activity--description of Paenibacillus uliginis sp. nov., an inhabitant of fen peat soil and Paenibacillus purispatii sp. nov., isolated from a spacecraft assembly clean room.	Behrendt U, Schumann P, Stieglmeier M, Pukall R, Augustin J, Sproer C, Schwendner P, Moissl-Eichinger C, Ulrich A	Syst Appl Microbiol	10.1016/j.syapm.2010.07.004	2010	*

References

#16640

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 22991

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#36040 ; Curators of the CIP;

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#80877

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID376136.1 )

#122063 Collection of Institut Pasteur ; Curators of the CIP; CIP 110057
* These data were automatically processed and therefore are not curated

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