Name: Paenibacillus purispatii ES_MS17
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 11653

Type strain:

Species: Paenibacillus purispatii

Strain Designation: ES_MS17, ES-MS17

Culture col. no.: DSM 22991, CIP 110057

Strain history: CIP <- 2009 <- R. Pukall, DSMZ, Germany <- C. Moisse-Eichinger: strain ES-MS17

NCBI tax ID(s): 556177 (species)

SI-ID 376136

CIP 110057, DSM 22991

Special Collections

DSMZ
CIP

Section

Paenibacillus purispatii ES_MS17 is a facultative anaerobe, Gram-positive, motile bacterium that was isolated from spacecraft assembly clean room at the European Space Research and Technology Centre .

Gram-positive
motile
rod-shaped
facultative anaerobe
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Paenibacillaceae*
	Genus	*Paenibacillus*
	Species	**Paenibacillus purispatii**
	Full Scientific Name (LPSN)	Paenibacillus purispatii Behrendt et al. 2011

Information on morphological and physiological properties Morphology

[Ref.: #122063]	Gram stain	positive

[Ref.: #122063]	Cell shape	rod-shaped

[Ref.: #122063]	Motility	yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #16640]

Culture medium

CASO AGAR (MERCK 105458) (DSMZ Medium 220)

[Ref.: #16640]

Culture medium growth

[Ref.: #16640]

Culture medium link

Medium recipe at MediaDive

[Ref.: #16640]

Culture medium composition

expand / minimize

[Ref.: #36040]

Culture medium

MEDIUM 72- for trypto casein soja agar

[Ref.: #36040]

Culture medium growth

[Ref.: #36040]

Culture medium composition

expand / minimize

[Ref.: #122063]

Culture medium

CIP Medium 72

[Ref.: #122063]

Culture medium growth

[Ref.: #122063]

Culture medium link

Medium recipe provided by DSMZ

	Temperatures	Kind of temperature		Temperature
[Ref.: #16640]			growth	30 °C
[Ref.: #36040]			growth	30 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #122063]

Oxygen tolerance

facultative anaerobe

	Metabolite utilization	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #122063]		nitrate ChEBI	-	reduction
[Ref.: #122063]		nitrite ChEBI	-	reduction

	Metabolite production	Metabolite	Production
[Ref.: #122063]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	+	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	+	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	+	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #122063]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #122063]	oxidase	+
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #122063]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #122063]	12331	-	-	+	+	-	+	-	-	-	+	-	+	-	+	-	+	+	-	-	+

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #16640]	Sample type/isolated from	spacecraft assembly clean room at the European Space Research and Technology Centre (ESTEC)
[Ref.: #16640]	Geographic location (country and/or sea, region)	Noordwijk
[Ref.: #16640]	Country	Netherlands
[Ref.: #16640]	Country ISO 3 Code	NLD
[Ref.: #16640]	Continent	Europe

[Ref.: #122063]	Sample type/isolated from	Room, clean
[Ref.: #122063]	Geographic location (country and/or sea, region)	Noorwijk
[Ref.: #122063]	Country	Netherlands
[Ref.: #122063]	Country ISO 3 Code	NLD
[Ref.: #122063]	Continent	Europe
[Ref.: #122063]	Isolation date	2007
* marker position based on {}

Isolation sources categories

#Engineered

#Built environment

#Clean room

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence EU888513 (>99% sequence identity) for Paenibacillus from Microbeatlas

Aquatic Samples
Soil Samples	4
Animal Samples	1
Plant Samples	5
Total Samples	10

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #16640]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #122063]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #16640]	Paenibacillus purispatii strain ES_MS17 16S ribosomal RNA gene, partial sequence	EU888513	1318	GenBank ENA	556177 tax ID

Availability in culture collections External links

[Ref.: #16640]

Culture collection no.

DSM 22991, CIP 110057

[Ref.: #80877]

SI-ID 376136

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Characterization of heterotrophic nitrifying bacteria with respiratory ammonification and denitrification activity--description of Paenibacillus uliginis sp. nov., an inhabitant of fen peat soil and Paenibacillus purispatii sp. nov., isolated from a spacecraft assembly clean room.	Behrendt U, Schumann P, Stieglmeier M, Pukall R, Augustin J, Sproer C, Schwendner P, Moissl-Eichinger C, Ulrich A	Syst Appl Microbiol	10.1016/j.syapm.2010.07.004	2010	*

References

#16640

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 22991

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#36040 ; Curators of the CIP;

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#80877

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID376136.1 )

#122063 Collection of Institut Pasteur ; Curators of the CIP; CIP 110057
* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Paenibacillus purispatii in external resources:
SILVA
BRENDA
PANGAEA
GBIF