Name: Thalassolituus oleivorans Mil-1
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 11294

Type strain:

Species: Thalassolituus oleivorans

Strain Designation: Mil-1

Culture col. no.: DSM 14913, LMG 21420

Strain history: <- P. N. Golyshin, GBF; Mil1 <- M. M. Yakimov and R. Denaro

NCBI tax ID(s): 187493 (species)

SI-ID 86370

LMG 21420, DSM 14913

Section

Thalassolituus oleivorans Mil-1 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from seawater, sediment interface.

coccus-shaped
Gram-negative
motile
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Oceanospirillales*
	Family	*Oceanospirillaceae*
	Genus	*Thalassolituus*
	Species	**Thalassolituus oleivorans**
	Full Scientific Name (LPSN)	Thalassolituus oleivorans Yakimov et al. 2004

Information on morphological and physiological properties Morphology

[Ref.: #29887]	Gram stain	negative

[Ref.: #29887]	Cell shape	coccus-shaped

[Ref.: #29887]	Motility	yes

[Ref.: #5457]

Incubation period

1-2 days

[Ref.: #29887]

Pigment production

yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #5457]

Culture medium

BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)

[Ref.: #5457]

Culture medium growth

[Ref.: #5457]

Culture medium link

Medium recipe at MediaDive

[Ref.: #5457]

Culture medium composition

expand / minimize

[Ref.: #5457]

Culture medium

ONR7a MEDIUM (DSMZ Medium 950)

[Ref.: #5457]

Culture medium growth

[Ref.: #5457]

Culture medium link

Medium recipe at MediaDive

[Ref.: #5457]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #5457]	growth	25 °C
[Ref.: #29887]	growth	04-30 °C
[Ref.: #29887]	optimum	20-25 °C

[Ref.: #5457]

Temperature range

mesophilic

	pH	Kind of pH		pH
[Ref.: #29887]			optimum	8
[Ref.: #29887]			growth	7.5-9

Information on physiology and metabolism Physiology and metabolism

[Ref.: #29887]

Oxygen tolerance

aerobe

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #29887]		NaCl		growth	0.5-5.7	%
[Ref.: #29887]		NaCl		optimum	02-03	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #29887]	acetate ChEBI	+	carbon source
[Ref.: #29887]	tween 20 ChEBI	+	carbon source
[Ref.: #29887]	tween 40 ChEBI	+	carbon source
[Ref.: #29887]	tween 80 ChEBI	+	carbon source

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #5457]	Sample type/isolated from	seawater, sediment interface
[Ref.: #5457]	Geographic location (country and/or sea, region)	Sicily, Milazzo harbor
[Ref.: #5457]	Country	Italy
[Ref.: #5457]	Country ISO 3 Code	ITA
[Ref.: #5457]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Marine
#Environmental	#Aquatic	#Sediment

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AJ431699 (>99% sequence identity) for Thalassolituus oleivorans subclade from Microbeatlas

Aquatic Samples	8475
Soil Samples	354
Animal Samples	968
Plant Samples	66
Total Samples	9863

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #5457]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #5457]	Thalassolituus oleivorans partial 16S rRNA gene, strain MIL-1	AJ431699	1366	ENA	1298593 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Thalassolituus oleivorans MIL-1	GCA_000355675	complete	GenBank	1298593 tax ID	*
[Ref.: #66792]	Thalassolituus oleivorans MIL-1	1298593.3	complete	PATRIC	1298593 tax ID	*
[Ref.: #66792]	Thalassolituus oleivorans MIL-1	2597489935	complete	JGI IMG/M	1298593 tax ID	*

[Ref.: #29887]

GC-content

53.2 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Thalassolituus oleivorans MIL-1 MIL-1 NCBI: GCA_000355675.1
[Ref.: #69480]	motile	yes	90.998	yes
[Ref.: #69480]	flagellated	yes	90.182	no
[Ref.: #69480]	gram-positive	no	98.898	yes
[Ref.: #69480]	anaerobic	no	98.354	yes
[Ref.: #69480]	aerobic	yes	85.974	yes
[Ref.: #69480]	halophile	yes	71.796	no
[Ref.: #69480]	spore-forming	no	96.561	no
[Ref.: #69480]	glucose-util	no	51.996	no
[Ref.: #69480]	thermophile	no	98.973	yes
[Ref.: #69480]	glucose-ferment	no	90.442	no

Availability in culture collections External links

[Ref.: #5457]

Culture collection no.

DSM 14913, LMG 21420

[Ref.: #80527]

SI-ID 86370

Literature:

Topic	Title	Authors	Journal	DOI	Year
Genetics	Genome Sequence of Thalassolituus oleivorans MIL-1 (DSM 14913T).	Golyshin PN, Werner J, Chernikova TN, Tran H, Ferrer M, Yakimov MM, Teeling H, Golyshina OV	Genome Announc	10.1128/genomeA.00141-13	2013	*
Phylogeny	Thalassolituus marinus sp. nov., a hydrocarbon-utilizing marine bacterium.	Choi A, Cho JC	Int J Syst Evol Microbiol	10.1099/ijs.0.046383-0	2012	*
Phylogeny	Thalassolituus oleivorans gen. nov., sp. nov., a novel marine bacterium that obligately utilizes hydrocarbons.	Yakimov MM, Giuliano L, Denaro R, Crisafi E, Chernikova TN, Abraham WR, Luensdorf H, Timmis KN, Golyshin PN	Int J Syst Evol Microbiol	10.1099/ijs.0.02424-0	2004	*

References

#5457

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 14913

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#29887

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26260 (see below)

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#80527

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID86370.1 )

#26260

IJSEM 141 2004 ( DOI 10.1099/ijs.0.02424-0 , PubMed 14742471 )

* These data were automatically processed and therefore are not curated

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Thalassolituus oleivorans MIL-1 MIL-1

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