Thalassolituus oleivorans (DSM 14913, LMG 21420, Mil-1)

Name: Thalassolituus oleivorans (DSM 14913, LMG 21420, Mil-1)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 11294

Type strain

Strain Designation Mil-1

Culture col. no. DSM 14913 LMG 21420 Mil-1

NCBI tax ID(s) 187493

Special Collections DSMZ Literature strains

History <- P. N. Golyshin, GBF; Mil1 <- M. M. Yakimov and R. Denaro

Links

Thalassolituus oleivorans Mil-1 is an aerobe, Gram-negative, motile bacterium that was isolated from seawater, sediment interface.

Gram-negative motile coccus-shaped aerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 86370

LMG 21420,DSM 14913

@ref 20215

Last LPSN update 2026-02-09 11:20:30

Domain Bacteria

Phylum Pseudomonadota

Class Gammaproteobacteria

Order Oceanospirillales

Family Oceanospirillaceae

Genus Thalassolituus

Species Thalassolituus oleivorans

Full scientific name Thalassolituus oleivorans Yakimov et al. 2004

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Confidence
29887	negative	coccus-shaped
125438	negative		98
125439	negative		99.1

Colony morphology

5457

Incubation period1-2 days

Pigmentation

29887

Productionyes

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
5457	BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)		Medium recipe at MediaDive	Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514; with strain-specific modifications) Composition: NaCl 19.45 g/l Tween 80 10.0 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
5457	ONR7a MEDIUM (DSMZ Medium 950)		Medium recipe at MediaDive	Name: ONR7a MEDIUM (DSMZ Medium 950) Composition: NaCl 22.79 g/l Agar 15.0 g/l Agarose 12.0 g/l MgCl2 x 6 H2O 11.18 g/l Na2SO4 3.98 g/l CaCl2 x 2 H2O 1.46 g/l TAPSO 1.3 g/l KCl 0.72 g/l NH4Cl 0.27 g/l Na2HPO4 x 7 H2O 0.089 g/l NaBr 0.083 g/l NaHCO3 0.031 g/l H3BO3 0.027 g/l SrCl2 x 6 H2O 0.024 g/l NaF 0.0026 g/l FeCl2 x 4 H2O 0.002 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
5457	positive	growth	25
29887	positive	growth	04-30
29887	positive	optimum	20-25

Culture pH

@ref	Ability	Type	PH	PH range
29887	positive	optimum	8
29887	positive	growth	7.5-9	alkaliphile

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
29887	aerobe
125439	obligate aerobe	92.1

Spore formation

@ref	Spore formation	Confidence
125438		95.165
125439		99.8

Halophily

@ref	Salt	Growth	Tested relation	Concentration
29887	NaCl	positive	growth	0.5-5.7 %
29887	NaCl	positive	optimum	02-03 %

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
29887	30089 ChEBI	acetate	+	carbon source
29887	53424 ChEBI	tween 20	+	carbon source
29887	53423 ChEBI	tween 40	+	carbon source
29887	53426 ChEBI	tween 80	+	carbon source

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Marine
#Environmental	#Aquatic	#Sediment

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
5457	seawater, sediment interface	Sicily, Milazzo harbor	Italy	ITA	Europe

Taxonmaps

69479

Global distribution of 16S sequence AJ431699 (>99% sequence identity) for Thalassolituus oleivorans subclade from Microbeatlas

Aquatic Samples	8475
Soil Samples	354
Animal Samples	968
Plant Samples	66
Total Samples	9863

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
5457	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM35567v1 assembly for Thalassolituus oleivorans MIL-1	complete	GCA_000355675	1298593.3	2597489935	1298593	98.59

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
5457	Thalassolituus oleivorans partial 16S rRNA gene, strain MIL-1	AJ431699	1366		1298593

GC content

29887

GC-content (mol%)53.2

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Thalassolituus oleivorans MIL-1
PATRIC: 1298593.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	99.80	no
125439	motility	BacteriaNetⓘ	yes	80.10	no
125439	gram_stain	BacteriaNetⓘ	negative	99.10	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	92.10	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Thalassolituus oleivorans MIL-1 MIL-1
NCBI: GCA_000355675.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	98.00	yes
125438	anaerobic	anaerobicⓘ	no	92.63	yes
125438	aerobic	aerobicⓘ	yes	80.96	no
125438	spore-forming	spore-formingⓘ	no	95.17	no
125438	thermophilic	thermophileⓘ	no	97.38	yes
125438	flagellated	motile2+ⓘ	yes	80.23	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Differential Protein Expression During Growth on Medium Versus Long-Chain Alkanes in the Obligate Marine Hydrocarbon-Degrading Bacterium Thalassolituus oleivorans MIL-1.	Gregson BH, Metodieva G, Metodiev MV, Golyshin PN, McKew BA.	Front Microbiol	10.3389/fmicb.2018.03130	2018
Metabolism	Analysis of storage lipid accumulation in Alcanivorax borkumensis: Evidence for alternative triacylglycerol biosynthesis routes in bacteria.	Kalscheuer R, Stoveken T, Malkus U, Reichelt R, Golyshin PN, Sabirova JS, Ferrer M, Timmis KN, Steinbuchel A.	J Bacteriol	10.1128/jb.01292-06	2007
Genetics	Genome Sequence of Thalassolituus oleivorans MIL-1 (DSM 14913T).	Golyshin PN, Werner J, Chernikova TN, Tran H, Ferrer M, Yakimov MM, Teeling H, Golyshina OV	Genome Announc	10.1128/genomeA.00141-13	2013
Phylogeny	Thalassolituus marinus sp. nov., a hydrocarbon-utilizing marine bacterium.	Choi A, Cho JC	Int J Syst Evol Microbiol	10.1099/ijs.0.046383-0	2012
Phylogeny	Thalassolituus oleivorans gen. nov., sp. nov., a novel marine bacterium that obligately utilizes hydrocarbons.	Yakimov MM, Giuliano L, Denaro R, Crisafi E, Chernikova TN, Abraham WR, Luensdorf H, Timmis KN, Golyshin PN	Int J Syst Evol Microbiol	10.1099/ijs.0.02424-0	2004

References

#5457	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 14913
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#26260	IJSEM 141 2004 ( DOI 10.1099/ijs.0.02424-0 , PubMed 14742471 )
#29887	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26260
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Thalassolituus oleivorans (DSM 14913, LMG 21420, Mil-1)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Pigmentation

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Metabolite utilization

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000355675

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

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